Yale’s Core Research Facilities provide Yale researchers access to state of the art scientific instrumentation, highly trained staff, and extraordinary consultants and specialists with the intent to keep Yale’s scientific research at the cutting edge. Within the School of Medicine, there are a number of services that make available research resources for internal investigators. Many of these resources are available to external investigators on a core by core basis.
Technology: Multiple confocal microscopy systems
Operational Model: Training and for user access to seven confocal systems including Airyscan, STED, Swept Field, Light Sheet and Super Resolution Microscopes. Software programs from multiple vendors are available for post-acquisition analysis including fluorescence deconvolution, 3D rendering, measurements of time lapse data and fluorescence co-localization.
More information about CCMI Confocal Microscopy
Contact: Al Mennone
(203) 785-3793
Technology:
Operational Model: staff-provided sample preparation and imaging, or training for independent imaging
More information about CCMI Bio-Electron Microscopy
Contact: Xinran Liu, PhD
(203) 785-4050
Services: Microscope imaging center: Imaging technologies (microscopes)-TIRF, Spinning Disk, STED & SIM
Services: PET scans and related services (radiosynthesis, metabolite analysis; ligand development, PK studies).
Services: All of the resources necessary for integrated studies of human brain function including research magnets and personnel for electrophysiological (1 dedicated EEG room) and psychological testing (3 behavioral testing rooms), computing, and image and data analysis. Dedicated to research activity.
Services: Cryo Microscopy & Imaging Core includes a cryomicrotome, and ultramicrotome, a Zeiss electron microscope, a Zeiss Axiophot epifluorescent photomicroscope and a Zeiss laser scanning confocal microscope. Technical staff provide support for users of this facility. Cryo Microscopy - Freeze a tissue sample, slice, mount, stain and return to customer. Usage of cryostat, cut cryostat sections, slided that are used for the cryostat
Technologies: Next-Gen Sequencing with multiple platforms (Illumina, Pacific Biosciences, 10X Genomics), Bioinformatics and microarrays for expression analysis, genotyping, miRNA and methylation analysis
Operational Model: Staff-provided services for Next-Generation Sequencing, Bioinformatics and Microarrays
More information about Yale Center for Genome Analysis
Contact: ycga@yale.edu
(203) 737-3031
Technology:
Operational Model: Staff-provided and Open Access
More information about Mass Spectrometry and Proteomics
Contact: TuKiet Lam
(203) 785-5086
Technology: Sanger sequencing services including individual samples, plated samples, PCR purification, fragment analysis, RT-PCR, and DNA and RNA QC Analysis
Operational Model: Staff-provided service
More information about DNA Sequencing
Contact: Amy Blanchard
dnasequencing@yale.edu
(203) 737-2566
Technology: Oligos including modified oligo and plate-based formats, custom cloning, RNA and methylated RNA, mixed bases, and purification
Operational Model: Staff-provided custom synthesis of oligonucleotides
More information about Oligo Synthesis Resource
Contact: Joe DeLuca
oligos@yale.edu
(203) 737-2541
Services: ShRNA and CRISPR/Cas9 screens in human and mouse cells. Resources include pooled libraries for screening, consultation and guidance in performing screens, and bioinformatics support for analysis of screening data. The core houses genome-wide collections of shRNA constructs and full length human ORF cDNAs that are available as individual constructs.
The YSCC Genomics Core Service provides DNA sequencing services, Transcriptome analysis (RNA-Seq), Small RNA discovery (smRNA-Seq), Gene regulation & epigenetic analysis (ChIP-Seq), Customized sequencing per user design and single cell genomics with Fluidigm C1 IFCs
Technology: Resources to study oligomeric state of biomolecular assemblies, as well as thermodynamics and kinetics of macromolecular interactions using:
Operational Model: Training and instrument access
More information about Keck Biophysical Resource
Contact: Ewa Folta-Stogniew
(203) 737-4387
Technologies: Protein characterization (MALS detector, Nano isothermal titration calorimeter), robotic crystallization preparation, crystallization imaging and growth monitoring, X-ray diffraction data collection, data processing and structure determination software.
Operational Model: Training and instrument access and staff-provided service
More information about Macromolecular X-ray Crystallography
Contact: Jim Murphy
(203) 906-5759
Technologies: YGEC creates genetically engineered mice via CRISPR/Cas genome editing, gene targeting via homologous recombination in ES cells, and traditional transgenic mouse technology. YGEC also cryopreserves sperm or embryos of inherently valuable genetically altered mouse strains. Assisted reproduction technologies include strain reanimation or rescue via in vitro fertilization and/or embryo transfer.
Operational Model: Staff-provided services
More information about Yale Genome Editing Center
Contact:
Technology: Expert resources to create transgenic zebrafish and provide assistance in phenotyping them.
Operational Model: Staff-provided service
More information about Zebrafish Phenotyping Core for Precision Medicine
Contact: Andrew Prendergast
(203) 843-0870
Animal housing husbandry complete veterinary services on the care and use of animals.
In vivo pharmacological evaluation of anti-cancer therapeutics. Services include efficacy, toxicology, pharmacokinetics, pharmacodynamics and imaging studies
Resources including staff support for a two-photon laser scanning microscope and surgical preparation area to perform time-resolved intravital imaging of rodents.
Small and large animal CT, SPECT-CT and ultrasound. Small animal laser Dopler flow. Large animal fluoroscopy. Pre- and post-operative animal care assistance associated with animal surgery.
More information about Yale Translational Research Imaging Center
Provides a variety of services that phenotype kidney function including assessing renal function in small animals at the level of single tubules in vitro and in vivo, the whole kidney, perfusion fixation for histology studies, and the intact organism.
More information about O'Brien Kidney Center-Renal Physiology Core
The Islet, Oxygen consumption, Mass Isotopomer flux core (IOMIC) measures islet function, oxygen consumption, and cellular metabolic flux
The Core uses a Hemavet veterinary hematology analyzer that provides accurate CBC counts from whole blood of mice, rats, dogs, and humans (research use only).
Enables irradiations of molecular compounds, cell culture, animal and human tumor cells, and whole animal experiments.
More information about Cesium irradiator and x-ray irradiators
Pathology support for animal-based studies to better understand disease processes and their treatment including comparative pathology, histology, necropsy and virology.
Testing of tissues, cells, feces and environmental samples for veterinary viruses, bacteria, parasites and fungi by PCR and RT-PCR. Biological contaminant testing of samples to be inoculated into rodents is required by IACUC and is free of charge to Yale principal investigators. Testing of mouse and rat sera for rodent viruses and Mycoplasma pulmonis.
More information about Molecular and Serological Diagnostics
This lab assesses the biomechanical properties of musculoskeletal tissues, providing services and technical assistance.
Services include chromosome karyotyping, cell and tissue culture (most organs, both human and non-human sources), fluorescence in situ hybridization, banding techniques (HRB, CBG, GTW, replication, SCE, and others), and advanced array comparative genomic hybridization (aCGH) diagnostics. Types of specimens processed include blood, amniotic fluid, chorionic villus, products of conception, bone marrow, skin and solid tumor samples from clinicians as well as various cell lines and tissues from researchers.
College of American Pathologists and CLIA certified laboratory with decades of experience in the molecular diagnosis of genetic disease. We provide testing for cancer predisposition, metabolic and developmental disorders. Provide GeneTests and custom re-sequencing assays.
Molecular Diagnostics by PCR and FISH tests including tests for clonal rearrangement, microsatellite instability, chromosomal segment deletion or translocation
Pathology support for animal-based studies to better understand disease processes and their treatment including comparative pathology, histology, necropsy and virology.
Process and Embed Tissue; Cut and Stain, 1st H&E Slide; Additional Cut Unstained Slide; Additional Cut and Stained H&E Slide; Special Stain; Immunohistochemistry; Immunofluorescence; 20X Slide Scan (Delivered on flash drive); 40X Slide Scan (Delivered on flash drive); 20X Slide Scan (PathPresenter-3mo. Max); 40X Slide Scan (PathPresenter-3mo. Max); In-Situ Hybridization (HPV High + Low); Frozen tissue mounting, cutting and staining
Operational Model: Staff-provided service
Contact: Dilgash Mekael, office #: 203-737-4363
The primary service provided is specialized processing, plastic embedding and cutting required for undecalcified tissues and biomaterial implants. Other services provided by this lab include routine paraffin embedding and sectioning, frozen sectioning, routine and special stains and immunohistochemical as well as enzyme histochemical stains. Also, histomorphometrical analyses of bone and other tissues are performed by this lab.
More information about Orthopædic Histology and Histomorphometry Laboratory
Embedding/Sectioning of Tissue, Stain. Tissue Microarray: Construction of Core Master Block, Slides from Tissue Microarray master blocks, Test Array. Antibody Validation, Antibody Titration, Quantification of Protein Expression in Tissue, In Situ Quantification of m-RNA Levels, Index Array Development
Technology: Resources for assay development and high throughput screening with small molecules in biochemical, cell-based and image-based assays (primarily 384-well format). Human CRISPR whole genome library available. Reformatting of samples in 96-, 384- and 1536-well plates. Platereading with a variety of modalities including FLIPR. High throughput image analysis.
Operational Model: Staff-provided service and Self Service Laboratory (subset of instruments)
More information about Yale Center for Molecular Discovery
Contact: Yulia Surovtseva
(203) 737-3271
Technology: Expert, custom services for custom design and fabrication, and repair, as well as tools for loan.
Operational Model: Staff-provided service including on-site and emergency service
More information about the Machine Shop
Contact: Tony DeSimone
(203) 785-4677
Genome editing service:
iPSC generation service
hESC/iPSC characterization service:
hESC/iPSC culture training service:
Reagents and cell line repository service:
CD34+ Cells from healthy human donors for use in research
Provide well characterized cultures of HUVEC, HECFC Cells, Media, placenta or cord blood, and media component for culturing endothelial cells.
More information about Tissue Culture Vascular Biology and Therapeutics
Provide the ethical collection, storage, annotation, and distribution of tissue and bodily fluids to support translational research for the field of Obstetrical and Gynecologic pathology.
More information about Yale University Reproductive Sciences Biobank