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Yale Flow Cytometry Facility

About Flow Cytometry

Our mission is to provide comprehensive flow cytometric analysis and sorting including instrumentation, technical support, training and consultation.

Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. Any suspended particle or cell from 0.5–150 micrometers in size is suitable for analysis. Cells from solid tissue must be disaggregated before analysis. Sorting allows us to capture and collect cells of interest for further analysis. Once collected, the cells can be analyzed microscopically, biochemically, or functionally.

To learn more, view Invitrogen's Introduction to Flow Cytometry tutorial.

Publication Acknowledgment

If research supported by this Research Core results in publication, please acknowledge this support by including the following in your publication(s):

"We thank Yale Flow Cytometry for their assistance with _________ service. The Core is supported in part by an NCI Cancer Center Support Grant # NIH P30 CA016359. The BD Symphony was funded by shared instrument grant # NIH S10 OD026996.”

Several improvements to the facility that are now available. 

  • Administration of a FlowJo site license for Yale.
  • Remote data access via Box@Yale. 
  • The Amistad FACSCalibur room 105 has been renovated in order to house both a Calibur and the Amistad LSRII. 
  • The Amistad Aria has been outfitted with a Baker BioProtect IV Safety cabinet.
  • The Amistad and LEPH sorters have both been upgraded to create equivalent laser and detector configurations.