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Enhanced FIB-SEM

At the forefront of volume Electron Microscopy (vEM), we welcome your collaboration to take discoveries in biology, physiology, and pathology to a whole new level using our proprietary enhanced Focused Ion Beam Scanning Electron Microscopy (eFIB-SEM) technology and its unparalleled pipeline.

Compared to conventional FIB-SEM, eFIB-SEM has expanded the maximum imageable volume by orders of magnitude and enabled much faster throughput, while maintaining the advantages of fine isotropic resolution (< 10 nm in x, y, and z) and robust image alignment (Xu et al., eLife 2017; US patent 10,600,615; Xu et al., Neuromethods 2020).


The enhanced FIB-SEM pipeline (Pang & Xu, Methods in Cell Biology 2023) has enabled transformational discoveries, featured in 3 Nature, 3 Science, 3 Cell, and 20 other publications from 2019 to 2023. This pipeline represents the beginning of a new field of high-resolution large volume electron microscopy to reveal the structure-function relationships in biology.

Explicitly, we advanced the technology and created the first open-access, 3D atlas of whole cells and tissues at the finest isotropic resolution of 4-nm voxels (Xu et al., Nature 2021), while Janelia scientists developed the first automatic reconstructions of 35 different cellular organelle classes (Heinrich et al., Nature 2021). Using these high resolution large volume FIB-SEM data, Hotamışlıgil Lab at Harvard linked liver subcellular architecture to metabolic function for the first time (Parlakgül et al., Nature 2022), and Mellman Lab at Genentech revealed the very first comprehensive 3D ultrastructure of a T cell attacking a cancer cell (Ritter et al., Science 2022). Explore more seminal discoveries through the Discovery Highlights and delve into the extensive details via our Publications.

Mouse liver from Lean to Obese with 8-nm voxels

Drosophila optical lobe with 4-nm voxels