Great News!!  Two new electron microscopes are coming to CCMI EM Facility in 2018

1)   Thanks to a NSF Major Research Instrument grant made to Yale University we are implementing a state-of-the-art Focused Ion Beam Scanning Electron Microscope (FIB-SEM) system for cellular tomography. The Zeiss Crossbeam 550 is a powerful and efficient tool to study the 3D structure and function of cells and their organelles - especially those that require high-resolution, yet large volumetric views. It will also allow new correlative studies that bridge the gap between light microscopy (including super-resolution) and electron microscopy. The installation of the instrument is expected to be completed in summer 2018.

2)   To meet growing demand for cryo electron microscopy on campus, the University has purchased a Thermo Scientific Glacios Cryo Transmission Electron Microscope. This newly released microscope features 200 kV XFEG optics, an autoloader and the same automation workflow as on the Titan Krios, it is also equipped with a K2 Summit Direct Electron Detector and Volta Phase Plate. The Glacios Cryo-TEM can used in a single particle analysis workflow for screening of sample quality before transferring grids to the Krios for ultimate data acquisition, or to collect high-quality image data directly. The instrument is scheduled to be delivered in August.

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Biological electron microscopy

The Biological EM facility is a full-service core facility that is administrated and supported by the Yale University School of Medicine. Our mission is to provide high-quality electron microscopy services with state-of-the-art techniques and equipment. We are open to all faculty, staff and students on entire Yale campus. The facility is located in the Sterling Hall of Medicine, Room IE26.

We offer a wide range of services from conventional and immuno- electron microscopy to electron tomography. Our experienced staff routinely perform sample processing, sectioning and image acquisition for a set fee. Alternatively, our facility is open to users who have been trained in electron microscopy. The training on use of electron microscope and sample preparation is provided on a one-on-one basis throughout the year.

People who wish to start an EM project and/or access to the facility equipment should contact facility director Xinran Liu at (203) 785-4050 or by email to xinran.liu@yale.edu.

Cryo electron microscopy

The Cryo EM Facility started in Departments of Molecular Biophysics and Biochemistry and Cellular and Molecular Physiology many years ago. To better support growing cryoEM demands, it has been integrated into CCMI EM facility as a YSM Central Core and service entirely cryo EM community at Yale.

Because of the complex nature of cryo EM projects and limitation of capacity, we do not offer direct services. Instead, interested investigators are encouraged to meet with facility staff to discuss feasibility of the project and training. In general, new users are trained by facility staff and expected to perform cryo EM project independently in the facility. Where necessary, additional training and assistance are available. 

For further information please contact Marc Llaguno at (203) 785-5902 or by email to marc.llaguno@yale.edu, or facility director Xinran Liu at (203) 785-4050 or by email to xinran.liu@yale.edu.

* In 2017 Yale University has implemented a Krios cryo electron microscope at West Campus, this high-end microscope is equipped with a Gatan GIF Quantum LS imaging filter and Volta phase plates. It has two direct electron detectors, a Falcon 3EC (pre-GIF) and a K2 summit camera (post-GIF) suitable for single particle or cryo electron tomography projects. For further information, please go to: https://cryoem.yale.edu or contact the facility director Shenping Wu at shenping.wu@yale.edu directly.

Acknowledgement

The CCMI EM Core Facility is administrated and financially supported by the dean's office of the Medical School. Your appropriate recognition will help us  continuously providing EM support to your research, please acknowledge our staff effort and/or use of the facility equipment in your publications. Thank you!