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Molecular and Serological Diagnostics

Molecular Diagnostics

Testing of tissues, cells, feces and environmental samples for veterinary viruses, bacteria, parasites and fungi by polymerase chain reaction (PCR) and reverse transcriptase-PCR (RT-PCR). Biological contaminant testing (BCT) of samples to be inoculated into rodents is required by IACUC and is free of charge to Yale principal investigators.

Sample submission: Samples should be collected using aseptic technique and stored at -70C until delivery to BML B33. The following sample types are accepted:

  • Cultured cells: 10^6 cells in a plastic tube with 1 ml of used media
  • Tumors or tissues: 100 mg
  • Feces: up to 6 pellets (mouse) per 1.5 ml tube
  • Environmental samples: up to 4 synthetic swabs in a 1.5 ml tube
Include a submission form containing a COA or PO # and contact information with your sample(s). Email Susan Compton for a submission form or to inquire about available assays.

Serological Diagnostics

Testing of mouse and rat sera for rodent viruses and Mycoplasma pulmonis

Sample submission: Store samples at -20C until delivery to BML B33. The following sample quantities are requested:

  • For 1-5 tests: submit 50 ul of undiluted serum or 500 ul of serum diluted 1:10 in saline
  • For 6-12 tess: submit 100 ul of undiluted serum or 1 ml of serum diluted 1:10 in saline
Do not submit whole blood.

Include a submission form containing a COA or PO # and contact information with your sample(s). Email Susan Compton for a submission form or to inquire about available assays.

Fees FY19

Service Internal Rate External for Profit External Non-Profit
PCR Test $62.08 $117.25 $106.91
Mouse BCT PCR Panel $346.89 $565.52 $597.40
Serology IFA Test $13.69 $25.91 $23.58