IOMIC

The Islet, Oxygen consumption, Mass Isotopomer flux Core (IOMIC) measures islet function, oxygen consumption, and cellular metabolic flux.

Contact Info:
Rebecca Cardone, M.S.
Co-director of the Yale IOMIC Flux Core
rebecca.cardone@yale.edu
Phone: (203) 785-7228

The islet cell biology component of the core offers isolation of islets, high-throughput parallel perfusion of islets, and dynamic studies of hormone secretion.

Click here to learn more about the Diabetes Research Center (DRC) Core.

Cost per plate:

Rodent Islet Isolation(s):

Single isolation -

• Internal - $631
• External (non-profit) - $1,102
• External - Please request quote

Price includes one isolation condition (1-2 control rats or 4 mice of same genotype) for one pooled batch of islets.

Multiple isolations -

• Internal - $831
• External (non-profit) - $1,462
• External - Please request quote

Price includes two maximum isolations per day (2-4 rats or 8 mice) for two separate batches of pooled islets from each of the two isolations.

Human or Rodent islet study:

• Internal - $1,705
• External (non-profit) - $2,958
• External - Please request quote

Price includes either an intact islet perfusion study or islet reaggregate dynamic static incubation study for hormone secretion, as well as ½ Insulin ELISA plate for islet study preliminary data and quality assurance.

Additional hormone ELISAs will be charged at cost depending on the type of hormone assay and number of ELISA plates required to run all desired samples. A typical glucose stimulated insulin secretion perifusion study requires 2 ELISA plates per study condition if all critical time points are assayed for first phase and second phase insulin secretion. A typical static glucose stimulated insulin secretion study requires ½ ELISA plate per study condition if a full glucose dose response curve is performed.

Costs associated with animal ordering & housing or human islet procurement & culture are not included in the islet isolation or islet study costs and will be charged separately to cover the costs associated with each add-on.

For sample submission please fill in this form.

As part of Yale Cancer Center (YCC) efforts to enhance membership access to metabolomics and metabolism technologies, the Kibbey laboratory will provide at cost steady state measurements of mitochondrial metabolism using the MIMOSA (Mass Isotopomer MultiOrdinate Spectral Analysis) platform (1). Consultative/collaborative design, application and interpretation of 13C labeled MIMOSA fluxomic measurements in cultured cells are key features of the service.

MIMOSA is a mass spectroscopy technique using stable isotopes to quantitatively measure the discrete rates of sequential individual reactions in glycolytic and mitochondrial metabolism. Such measurements can be used to identify differences in fuel usage (e.g., glycolysis vs. beta-oxidation), non-oxidative (reductive carboxylation, anaplerosis and cataplerosis) contributions to the TCA cycle. Additional development of the resource for the analysis of other metabolic fluxes may be possible.

Please Note: The typical turnaround time for data from these services is on average 2-3 months for the initial analysis but can vary depending on laboratory work load and the complexity of the study. For advanced metabolomics analysis or integrative -omics analysis, please allow for an additional 1-3 months depending on the number of comparisons.

Alves TC, Pongratz RL, Zhao X, Yarborough O, Sereda S, Shirihai O, Cline GW, Mason G, Kibbey R, (2015) Integrated, step-wise, mass-isotopomeric flux analysis of the TCA cycle. Cell Metab. 2015 Nov 3;22(5):936-47. doi: 10.1016/j.cmet.2015.08.021. Epub 2015 Sep 24. PMID: 26411341

Click here to learn more about the Yale Cancer Center.

LC/MS/MS MIMOSA Study:

• Internal - $92/sample
• External (non-profit) - $169/sample

Sample cost includes LC/MS/MS sample plate preparation, plate run, peak integration and data processing/analysis/interpretation including isotopologue enrichment with appropriate phi calculations and metabolite concentration data.

Additional costs:

Full-service labeling study -

• Internal: $846
• External (non-profit): $1488
• External - Please request quote

The full-service labeling study is a one-time fee per study day including the stable isotopes [U13C-glucose, 1,2-13C-glutamine or U13C-lactate], the dynamic or steady state labeling experiment and cell quenching in preparation for the LC/MS/MS MIMOSA run. This service does not include but is in addition to the LC/MS/MS study run as a cost per sample described above.

For dynamic metabolic flux studies, it is strongly recommended that a pilot time course experiment be performed prior to the full LC/MS/MS study in order to determine sample sensitivity on the instrument and to determine metabolic and isotopic steady state for the particular cell samples. Please inquire for discounted pricing on the pilot experiment.

Method development-

• Internal: $385/day
• External (non-profit): $740/day
• External: Please request quote

An additional fee for method development of new metabolites not in our MIMOSA central carbon metabolite panel will be applied if required. Generally, 3 new metabolite standards can be optimized per day. Standards are to be provided by the customer for this service.

Study assistance -

• Internal: $100/hour
• External (non-profit): $182/hour
• External - Please request quote

For additional sample preparation assistance that does not fall under the MIMOSA services described above is based on a per hour fee. Additional study assistance is required for samples other than cells submitted in the standard 6-well plate that require extra time-consuming steps such as rodent plasma/tissue processing from stable isotope label infusion studies or unlabeled tissues/plasma for metabolite concentrations.

For sample submission please fill in this form . The protocols for the labeling studies can be found here.

LC/MS/MS Global Metabolomics and IntOmics studies:

4-mode metabolomics -

• Internal - $162/sample
• External (non-profit) - $315/sample
• External - Please request quote

This service is our standard targeted and untargeted global metabolomics platform. Each sample is run on 2 different liquid chromatography columns (Hypercarb and Reverse Phase) in both positive and negative modes for a total of 4 sample runs per study sample providing our most complete metabolomic coverage. Sample costs include LC/MS/MS supplies, LC/MS/MS sample plate preparation, sample plate run, peak integration and data processing with basic data analysis and data interpretation. The basic analysis included in the LC/MS/MS cost per sample includes PCA and PLSDA plots, heatmaps and 50 top metabolites based on a p value of <0.05 when 2 cohorts are compared (1x1 comparison).

2-mode metabolomics -

• Internal - $108/sample
• External (non-profit) - $204/sample
• External - Please request quote

This option offers a cost savings for targeted and untargeted global metabolomics but does not provide full coverage. Each sample is run in 2 modes only, typically Hypercarb negative and Reverse Phase positive with only 2 sample runs per study sample. This service is recommended if only certain metabolites are of interest and can also be measured in just 2 modes (please inquire). Sample costs include LC/MS/MS supplies, LC/MS/MS sample plate preparation, sample plate run, peak integration and data processing with basic data analysis and data interpretation. The basic analysis included in the LC/MS/MS cost per sample includes PCA and PLSDA plots, heatmaps and 50 top metabolites based on a p value of <0.05 when 2 cohorts are compared (1x1 comparison).

Additional costs:

Advanced data analysis (1 comparison)

• Internal: $300/analysis
• External (non-profit): $542/analysis
• External - Please request quote

Advanced in-depth data analysis beyond what is offered in the basic analysis includes volcano plots, pathway enrichment and KEGG network optimization data for each 1x1 comparison of study cohorts (i.e. Ctrl vs drug, WT vs Mut, etc). The price for this analysis is based on the number of comparisons desired, where each comparison takes approximately 3-4 hours.

Advanced data analysis with IntOmics -

• Internal: $600/analysis
• External (non-profit): $1057/analysis
• External - Please request quote

Advanced data analysis with IntOmics provides everything that is offered in the metabolomics advanced data analysis described above in addition to in-depth Intomics data analysis merging metabolomics data with RNAseq data (provided by user). This additional service provides upstream processing of raw RNAseq data for quality assurance, alignment, and quantification. These data are then merged with the metabolomics data on an IntOmix platform providing the additional transcript information to the metabolomics network optimization.

Method development-

• Internal: $385/day
• External (non-profit): $740/day
• External: Please request quote

An additional fee for method development of new metabolites not currently in our standard targeted metabolite library will be applied if required. Generally, 3 new metabolite standards can be optimized per day. Standards are to be provided by the customer for this service.

Study assistance -

• Internal: $100/hour
• External (non-profit): $182/hour
• External - Please request quote

For additional sample preparation assistance that does not fall under the global metabolomics services described above is based on a per hour fee. Additional study assistance is required for samples other than cells submitted in the standard 6-well plate that require extra time-consuming steps such as rodent plasma/tissue processing.

For sample submission please fill in this form.

The XF Analyzer is the first and only commercially available instrument for scientific research that simultaneously measures in real-time and in live cells the two major energy pathways of the cell: mitochondrial respiration and glycolysis. Since 2007 when its patent was issued, the XF Analyzer has been used in laboratories world-wide to more rapidly and more accurately obtain real-time measurements of cellular respiration, oxidative phosphorylation, motility, and transport pathways. Such measurements would be invaluable in gaining insights into the mechanisms of many diseases, including cancer, that are linked to metabolic dysfunction or reprogramming. Please follow the links for more information about Seahorse XF Analyzer and measuring mitochondrial respiration.

Using Seahorse XF Analyzers, we can readily obtain novel and important data from live cells in real time, thereby providing a greater understanding of cell metabolism, and enabling new advancements in life science research.

Learn more about the supporting centers here: Yale Cancer Center and Diabetes Research Center.

Seahorse Study:

• Internal : $235/4 hour block
  
• External (non-profit): $299/4 hour block
• External : Please request quote

Study Assistance:

• Internal: $100/hour
• External (non-profit): $182/hour
• External - Please request quote

Additional study assistance is required for full-service oxygen consumption and extracellular acidification studies, DNA data normalization and data analysis based on a per hour fee.

All consumables are provided by the user or will be charged at cost in addition to the block charge and any additional study assistance fees. For pilot study guidelines and experimental design click here.

• For instrument use please fill in this form.
• After submitting the previous form, time slots can be reserved through this link.