IOMIC Flux Core

The Islet, Oxygen consumption, Mass Isotopomer flux Core (IOMIC) measures islet function, oxygen consumption, and cellular metabolic flux.

Contact Info:
Rebecca Cardone, M.S.
Co-director of the Yale IOMIC Flux Core
rebecca.cardone@yale.edu
Phone: (203) 785-7228

The islet cell biology component of the core offers isolation of islets, high-throughput parallel perfusion of islets, and dynamic studies of hormone secretion. 

Click here to learn more about the Diabetes Research Center (DRC) Core.

Cost per plate: 

Intact islet perfusion study: 

• Internal - $1,299/plate 
• External (non-profit) - $2,239/plate
• External - $2,518/plate 

Price includes islet isolation, islet glucose stimulated insulin secretion perfusion study and hormone secretion assay. A typical glucose stimulated insulin secretion study requires 2 assay plates if all critical time points are assayed for first phase and second phase insulin secretion. Costs associated with animal ordering & housing, human islet procurement & culture or additional study substrates/secretagogues are not included in the cost per plate and will be charged separately to cover the costs associated with each add-on.

Islet re-aggregate (pseudo-islet) study: 

• Internal - $1,850/plate 
• External (non-profit) - $3,187/plate
• External - $3,719/plate 
  

Price includes islet isolation, islet dissociation/re-aggregation, dynamic glucose stimulated insulin secretion study and insulin assay. A typical dynamic glucose stimulated insulin secretion study in control/vehicle versus 1-2 study conditions/drug treatments is equivalent to 1 islet re-aggregate study plate. Costs associated with animal ordering & housing, human islet procurement & culture or additional study substrates/secretagogues are not included in the cost per plate and will be charged separately to cover the costs associated with each add-on.

As part of Yale Cancer Center (YCC) efforts to enhance membership access to metabolomics and metabolism technologies, the Kibbey laboratory will provide at cost steady state measurements of mitochondrial metabolism using the MIMOSA (Mass Isotopomer MultiOrdinate Spectral Analysis) platform (1). Consultative/collaborative design, application and interpretation of 13C labeled MIMOSA fluxomic measurements in cultured cells are key features of the service. 

MIMOSA is a mass spectroscopy technique using stable isotopes to quantitatively measure the discrete rates of sequential individual reactions in glycolytic and mitochondrial metabolism. Such measurements can be used to identify differences in fuel usage (e.g., glycolysis vs. beta-oxidation), non-oxidative (reductive carboxylation, anaplerosis and cataplerosis) contributions to the TCA cycle. Additional development of the resource for the analysis of other metabolic fluxes may be possible.

Alves TC, Pongratz RL, Zhao X, Yarborough O, Sereda S, Shirihai O, Cline GW, Mason G, Kibbey R, (2015) Integrated, step-wise, mass-isotopomeric flux analysis of the TCA cycle. Cell Metab. 2015 Nov 3;22(5):936-47. doi: 10.1016/j.cmet.2015.08.021. Epub 2015 Sep 24. PMID: 26411341

Click here to learn more about the Yale Cancer Center.

LC/MS/MS study:

• Internal - $2,085/plate 
• External (non-profit) - $3,591/plate
  
• External - $4,144/plate 

  Plate cost includes LC/MS/MS plate preparation, plate run, peak integration and data processing/analysis/interpretation.

Additional costs:

• Method development-
    Internal: $189/metabolite 
    External (non-profit): $327/metabolite
    External: $372/metabolite 
  

• Study assistance -
    Internal: $79/hour
    External (non-profit): $137/hour
    External - $157/hour
  

• Full service labeling study - 
    Internal: $544/day
    External (non-profit): $939/day
    External - $1,060/day     
  

It is strongly recommended that a pilot time course experiment be performed prior to the full LC/MS/MS study in order to determine sample sensitivity on the instrument and to determine metabolic and isotopic steady state for the particular test samples. The pilot study will be charged as a half study plate.

For sample submission please fill in this form.  The protocols for the labeling studies can be found here.

The XF Analyzer is the first and only commercially available instrument for scientific research that simultaneously measures in real-time and in live cells the two major energy pathways of the cell: mitochondrial respiration and glycolysis. Since 2007 when its patent was issued, the XF Analyzer has been used in laboratories world-wide to more rapidly and more accurately obtain real-time measurements of cellular respiration, oxidative phosphorylation, motility, and transport pathways. Such measurements would be invaluable in gaining insights into the mechanisms of many diseases, including cancer, that are linked to metabolic dysfunction or reprogramming. Please follow the links for more information about Seahorse XF Analyzer and measuring mitochondrial respiration.

Using Seahorse XF Analyzers, we can readily obtain novel and important data from live cells in real time, thereby providing a greater understanding of cell metabolism, and enabling new advancements in life science research.

Learn more about the supporting centers here: Yale Cancer Center and Diabetes Research Center.

Seahorse Study:

• Internal (YCC or DRC members): $150/4 hour block
• Internal : $300/4 hour block
  
  
• External (non-profit): $521/4 hour block
• External : $607/4 hour block

All consumables are provided by the user or will be charged at cost in addition to the block charge. For pilot study guidelines and experimental design click here.

• For instrument use please fill in this form. 
• After submitting the previous form, time slots can be reserved through this link.