As part of Yale Cancer Center (YCC) efforts to enhance membership access to metabolomics and metabolism technologies, the Kibbey laboratory will provide at cost steady state measurements of mitochondrial metabolism using the MIMOSA (Mass Isotopomer MultiOrdinate Spectral Analysis) platform (1). Consultative/collaborative design, application and interpretation of 13C labeled MIMOSA fluxomic measurements in cultured cells are key features of the service.
MIMOSA is a mass spectroscopy technique using stable isotopes to quantitatively measure the discrete rates of sequential individual reactions in glycolytic and mitochondrial metabolism. Such measurements can be used to identify differences in fuel usage (e.g., glycolysis vs. beta-oxidation), non-oxidative (reductive carboxylation, anaplerosis and cataplerosis) contributions to the TCA cycle. Additional development of the resource for the analysis of other metabolic fluxes may be possible.
Alves TC, Pongratz RL, Zhao X, Yarborough O, Sereda S, Shirihai O, Cline GW, Mason G, Kibbey R, (2015) Integrated, step-wise, mass-isotopomeric flux analysis of the TCA cycle. Cell Metab. 2015 Nov 3;22(5):936-47. doi: 10.1016/j.cmet.2015.08.021. Epub 2015 Sep 24. PMID: 26411341
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- Internal - $2056.03/plate
- External - $3951.63/plate
- Method development - $330.22/day
- Full service labeling study for MIMOSA - $552/study
- Study assistance -
- $66.79/hour internal
- $132.74/hour external
Plate cost includes LC/MS/MS plate preparation, plate run, peak integration and data processing/analysis/interpretation. Costs help cover a small fraction of the annual service contract for the LC/MS/MS, gas generator and HPLC maintenance. An additional fee for method development will be applied if required. Study assistance fees will be applied in addition to the LC/MS/MS plate cost for performing the stable isotopic labeling study in cells or additional sample preparation if necessary. It is strongly recommended that a pilot experiment be performed prior to the full MIMOSA in order to determine the study specific protocol, as well as perform a time course to determine steady state. This particular service will be charged as half of the standard plate cost.
For sample submission please fill in this form. The protocols for the labeling studies can be found here.