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Past Workshops

Yale Center for Cellular and Molecular Imaging: Innovations in Visualization and Optical Imaging of Fixed and Living Cells and Tissues

Tuesday, March 12, 2019, 1:00–5:00 p.m. 
The Anlyan Center Auditorium, N143 
300 Cedar St., New Haven

Confocal fluorescence microscopy is the most widely used method for advanced light microscopic imaging of biological specimens. However, several new technologies now permit fluorescence microscopic imaging that is either faster than standard (pointscanning) confocal microscopy, has better spatial resolution, or both. Rapid image collection techniques, which are ideal for examining live cells, tissues, and organisms, including swept field, multiphoton, and Light Sheet microscopy will be highlighted at the workshop. Super-resolution techniques, which can result in 2- to 10- fold improvement in spatial resolution relative to standard confocal microscopy, including gated STED, singlemolecule localization, and Airyscan microscopy, will also be discussed. The workshop will illustrate the use of these new imaging techniques, each of which is now available in the medical school’s Center for Cell and Molecular Imaging.


1:00 p.m.  Welcoming remarks

Introduction to Workshop

Michael C. Crair, PhD Deputy Dean for Scientific Affairs (Basic Science Departments); William Ziegler III Professor of Neuroscience; and Professor of Ophthalmology & Visual Science

1:15–1:45 pm Overview of Center for Cellular and Molecular Imaging (CCMI)

Michael Nathanson, MD, PhD Gladys Phillips Crofoot Professor of Medicine and Professor of Cell Biology; Director, Yale Liver Center; Director, Yale Center for Cellular and Molecular Imaging

1:45–2:45 pm Short Talks on Live Cell Imaging by Yale CCMI Users

  • Swept Field Confocal Microscopy: Analyzing Genes and Mechanisms Leading to Human Birth Defects 
    Engin Deniz, MD Assistant Professor of Pediatrics, Khokha Lab 
  • Multiphoton Microscopy: Two-Photon Microscopy for Structural and Functional Imaging in Deep Tissue 
    Daniel Barson MD-PhD student in Neuroscience, Higley & Crair Labs 
  • Light Sheet Microscopy: Imaging of Intertissue Adhesion Using Light Sheet and Confocal Microscopy 
    Emilie Guillon, PhD Postdoctoral Associate in Molecular, Cellular, and Developmental Biology, Holley Lab

2:45 pm Coffee, Tea, and Cookies

3:00–4:00 pm Short Talks on Super Resolution Imaging by Yale CCMI Users

  • Gated STED Microscopy: Investigating Nanoscale Structures of the ER with Live STED 
    Lena Schroeder, PhD Associate Research Scientist Cell Bio, Bewersdorf Lab 
  • Single-Molecule Localization Imaging: Using Super Resolution Microscopy to Resolve Hair Cell Physiology and Structure 
    Joe Santos Sacchi, PhD Professor of Surgery (Otolaryngology), of Cellular and Molecular Physiology, and of Neuroscience 
  • Airyscan-Based Imaging: Examining Cytokinetic Nodes in Fission Yeast 
    Wasim Sayyad, PhD Postdoctoral Associate in Molecular, Cellular, and Developmental Biology, Pollard Lab

4:00–5:00 pm Keynote Speaker

Walking the Genome at Super-resolution

Ting (Chao-ting) Wu, PhD Professor of Genetics, Harvard Medical School

5:00 pm Closing Remarks

Michael H. Nathanson, MD, PhD Gladys Phillips Crofoot Professor of Medicine and Professor of Cell Biology; Director, Yale Liver Center; Director, Yale Center for Cellular and Molecular Imaging