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Pradeep Uchil, PhD

Research Scientist

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Selected Publications

Architecture of the Flaviviral Replication Complex PROTEASE, NUCLEASE, AND DETERGENTS REVEAL ENCASEMENT WITHIN DOUBLE-LAYERED MEMBRANE COMPARTMENTS*Uchil P, Satchidanandam V. Architecture of the Flaviviral Replication Complex PROTEASE, NUCLEASE, AND DETERGENTS REVEAL ENCASEMENT WITHIN DOUBLE-LAYERED MEMBRANE COMPARTMENTS*. Journal Of Biological Chemistry 2003, 278: 24388-24398. PMID: 12700232, DOI: 10.1074/jbc.m301717200.
Phylogenetic analysis of Japanese encephalitis virus: envelope gene based analysis reveals a fifth genotype, geographic clustering, and multiple introductions of the virus into the Indian subcontinent.Uchil P, Satchidanandam V. Phylogenetic analysis of Japanese encephalitis virus: envelope gene based analysis reveals a fifth genotype, geographic clustering, and multiple introductions of the virus into the Indian subcontinent. American Journal Of Tropical Medicine And Hygiene 2001, 65: 242-51. PMID: 11561712, DOI: 10.4269/ajtmh.2001.65.242.
The Fc-effector function of COVID-19 convalescent plasma contributes to SARS-CoV-2 treatment efficacy in miceUllah I, Beaudoin-Bussières G, Symmes K, Cloutier M, Ducas E, Tauzin A, Laumaea A, Grunst M, Dionne K, Richard J, Bégin P, Mothes W, Kumar P, Bazin R, Finzi A, Uchil P. The Fc-effector function of COVID-19 convalescent plasma contributes to SARS-CoV-2 treatment efficacy in mice. Cell Reports Medicine 2022, 4: 100893. PMID: 36584683, PMCID: PMC9799175, DOI: 10.1016/j.xcrm.2022.100893.
In vivo imaging of retrovirus infection reveals a role for Siglec-1/CD169 in multiple routes of transmissionHaugh KA, Ladinsky MS, Ullah I, Stone HM, Pi R, Gilardet A, Grunst MW, Kumar P, Bjorkman PJ, Mothes W, Uchil PD. In vivo imaging of retrovirus infection reveals a role for Siglec-1/CD169 in multiple routes of transmission. ELife 2021, 10: e64179. PMID: 34223819, PMCID: PMC8298093, DOI: 10.7554/elife.64179.
Live imaging of SARS-CoV-2 infection in mice reveals that neutralizing antibodies require Fc function for optimal efficacyUllah I, Prévost J, Ladinsky MS, Stone H, Lu M, Anand SP, Beaudoin-Bussières G, Symmes K, Benlarbi M, Ding S, Gasser R, Fink C, Chen Y, Tauzin A, Goyette G, Bourassa C, Medjahed H, Mack M, Chung K, Wilen CB, Dekaban GA, Dikeakos JD, Bruce EA, Kaufmann DE, Stamatatos L, McGuire AT, Richard J, Pazgier M, Bjorkman PJ, Mothes W, Finzi A, Kumar P, Uchil PD. Live imaging of SARS-CoV-2 infection in mice reveals that neutralizing antibodies require Fc function for optimal efficacy. Immunity 2021, 54: 2143-2158.e15. PMID: 34453881, PMCID: PMC8372518, DOI: 10.1016/j.immuni.2021.08.015.
A Protective Role for the Lectin CD169/Siglec-1 against a Pathogenic Murine RetrovirusUchil PD, Pi R, Haugh KA, Ladinsky MS, Ventura JD, Barrett BS, Santiago ML, Bjorkman PJ, Kassiotis G, Sewald X, Mothes W. A Protective Role for the Lectin CD169/Siglec-1 against a Pathogenic Murine Retrovirus. Cell Host & Microbe 2018, 25: 87-100.e10. PMID: 30595553, PMCID: PMC6331384, DOI: 10.1016/j.chom.2018.11.011.
Retroviruses use CD169-mediated trans-infection of permissive lymphocytes to establish infectionSewald X, Ladinsky MS, Uchil PD, Beloor J, Pi R, Herrmann C, Motamedi N, Murooka TT, Brehm MA, Greiner DL, Shultz LD, Mempel TR, Bjorkman PJ, Kumar P, Mothes W. Retroviruses use CD169-mediated trans-infection of permissive lymphocytes to establish infection. Science 2015, 350: 563-567. PMID: 26429886, PMCID: PMC4651917, DOI: 10.1126/science.aab2749.
TRIM5 is an innate immune sensor for the retrovirus capsid latticePertel T, Hausmann S, Morger D, Züger S, Guerra J, Lascano J, Reinhard C, Santoni FA, Uchil PD, Chatel L, Bisiaux A, Albert ML, Strambio-De-Castillia C, Mothes W, Pizzato M, Grütter MG, Luban J. TRIM5 is an innate immune sensor for the retrovirus capsid lattice. Nature 2011, 472: 361-365. PMID: 21512573, PMCID: PMC3081621, DOI: 10.1038/nature09976.
TRIM E3 Ligases Interfere with Early and Late Stages of the Retroviral Life CycleUchil PD, Quinlan BD, Chan WT, Luna JM, Mothes W. TRIM E3 Ligases Interfere with Early and Late Stages of the Retroviral Life Cycle. PLOS Pathogens 2008, 4: e16. PMID: 18248090, PMCID: PMC2222954, DOI: 10.1371/journal.ppat.0040016.
TRIM15 is a focal adhesion protein that regulates focal adhesion disassemblyUchil PD, Pawliczek T, Reynolds TD, Ding S, Hinz A, Munro JB, Huang F, Floyd RW, Yang H, Hamilton WL, Bewersdorf J, Xiong Y, Calderwood DA, Mothes W. TRIM15 is a focal adhesion protein that regulates focal adhesion disassembly. Journal Of Cell Science 2014, 127: 3928-3942. PMID: 25015296, PMCID: PMC4163643, DOI: 10.1242/jcs.143537.
HIV-1 Env trimers asymmetrically engage CD4 receptors in membranesLi W, Qin Z, Nand E, Grunst M, Grover J, Bess J, Lifson J, Zwick M, Tagare H, Uchil P, Mothes W. HIV-1 Env trimers asymmetrically engage CD4 receptors in membranes. Nature 2023, 623: 1026-1033. PMID: 37993716, PMCID: PMC10686830, DOI: 10.1038/s41586-023-06762-6.
Immunogenicity and Pre-Clinical Efficacy of an OMV-Based SARS-CoV-2 VaccineGrandi A, Tomasi M, Ullah I, Bertelli C, Vanzo T, Accordini S, Gagliardi A, Zanella I, Benedet M, Corbellari R, Di Lascio G, Tamburini S, Caproni E, Croia L, Ravà M, Fumagalli V, Di Lucia P, Marotta D, Sala E, Iannacone M, Kumar P, Mothes W, Uchil P, Cherepanov P, Bolognesi M, Pizzato M, Grandi G. Immunogenicity and Pre-Clinical Efficacy of an OMV-Based SARS-CoV-2 Vaccine. Vaccines 2023, 11: 1546. PMID: 37896949, PMCID: PMC10610814, DOI: 10.3390/vaccines11101546.
Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetryLeonhardt S, Purdy M, Grover J, Yang Z, Poulos S, McIntire W, Tatham E, Erramilli S, Nosol K, Lai K, Ding S, Lu M, Uchil P, Finzi A, Rein A, Kossiakoff A, Mothes W, Yeager M. Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry. Nature Communications 2023, 14: 4368. PMID: 37474505, PMCID: PMC10359404, DOI: 10.1038/s41467-023-39262-2.
PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infectionXu D, Jiang W, Wu L, Gaudet R, Park E, Su M, Cheppali S, Cheemarla N, Kumar P, Uchil P, Grover J, Foxman E, Brown C, Stansfeld P, Bewersdorf J, Mothes W, Karatekin E, Wilen C, MacMicking J. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. Nature 2023, 619: 819-827. PMID: 37438530, PMCID: PMC10371867, DOI: 10.1038/s41586-023-06322-y.
Vaccinia Virus Strain MVA Expressing a Prefusion-Stabilized SARS-CoV-2 Spike Glycoprotein Induces Robust Protection and Prevents Brain Infection in Mouse and Hamster ModelsLorenzo M, Marín-López A, Chiem K, Jimenez-Cabello L, Ullah I, Utrilla-Trigo S, Calvo-Pinilla E, Lorenzo G, Moreno S, Ye C, Park J, Matía A, Brun A, Sánchez-Puig J, Nogales A, Mothes W, Uchil P, Kumar P, Ortego J, Fikrig E, Martinez-Sobrido L, Blasco R. Vaccinia Virus Strain MVA Expressing a Prefusion-Stabilized SARS-CoV-2 Spike Glycoprotein Induces Robust Protection and Prevents Brain Infection in Mouse and Hamster Models. Vaccines 2023, 11: 1006. PMID: 37243110, PMCID: PMC10220993, DOI: 10.3390/vaccines11051006.
Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBDChen Y, Prévost J, Ullah I, Romero H, Lisi V, Tolbert W, Grover J, Ding S, Gong S, Beaudoin-Bussières G, Gasser R, Benlarbi M, Vézina D, Anand S, Chatterjee D, Goyette G, Grunst M, Yang Z, Bo Y, Zhou F, Béland K, Bai X, Zeher A, Huang R, Nguyen D, Sherburn R, Wu D, Piszczek G, Paré B, Matthies D, Xia D, Richard J, Kumar P, Mothes W, Côté M, Uchil P, Lavallée V, Smith M, Pazgier M, Haddad E, Finzi A. Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD. IScience 2022, 26: 105783. PMID: 36514310, PMCID: PMC9733284, DOI: 10.1016/j.isci.2022.105783.
OP 6.2 – 00195 Targeted genome engineering of human t cells in vivo for HIV cureKumar P, Beloor J, Krishnaswamy J, Ullah I, Uchil P. OP 6.2 – 00195 Targeted genome engineering of human t cells in vivo for HIV cure. Journal Of Virus Eradication 2022, 8: 100247. DOI: 10.1016/j.jve.2022.100247.
Engineered ACE2-Fc counters murine lethal SARS-CoV-2 infection through direct neutralization and Fc-effector activitiesChen Y, Sun L, Ullah I, Beaudoin-Bussières G, Anand SP, Hederman AP, Tolbert WD, Sherburn R, Nguyen DN, Marchitto L, Ding S, Wu D, Luo Y, Gottumukkala S, Moran S, Kumar P, Piszczek G, Mothes W, Ackerman ME, Finzi A, Uchil PD, Gonzalez FJ, Pazgier M. Engineered ACE2-Fc counters murine lethal SARS-CoV-2 infection through direct neutralization and Fc-effector activities. Science Advances 2022, 8: eabn4188. PMID: 35857504, PMCID: PMC9278865, DOI: 10.1126/sciadv.abn4188.
VE607 stabilizes SARS-CoV-2 Spike in the “RBD-up” conformation and inhibits viral entryDing S, Ullah I, Gong SY, Grover J, Mohammadi M, Chen Y, Vézina D, Beaudoin-Bussières G, Verma VT, Goyette G, Gaudette F, Richard J, Yang D, Smith AB, Pazgier M, Côté M, Abrams C, Kumar P, Mothes W, Uchil P, Finzi A, Baron C. VE607 stabilizes SARS-CoV-2 Spike in the “RBD-up” conformation and inhibits viral entry. IScience 2022, 25: 104528. PMID: 35677392, PMCID: PMC9164512, DOI: 10.1016/j.isci.2022.104528.
Fc effector cross-reactivity: A hidden arsenal against SARS-CoV-2’s evasive maneuveringGrunst MW, Uchil PD. Fc effector cross-reactivity: A hidden arsenal against SARS-CoV-2’s evasive maneuvering. Cell Reports Medicine 2022, 3: 100540. PMID: 35233551, PMCID: PMC8823971, DOI: 10.1016/j.xcrm.2022.100540.
A Fc-enhanced NTD-binding non-neutralizing antibody delays virus spread and synergizes with a nAb to protect mice from lethal SARS-CoV-2 infectionBeaudoin-Bussières G, Chen Y, Ullah I, Prévost J, Tolbert WD, Symmes K, Ding S, Benlarbi M, Gong SY, Tauzin A, Gasser R, Chatterjee D, Vézina D, Goyette G, Richard J, Zhou F, Stamatatos L, McGuire AT, Charest H, Roger M, Pozharski E, Kumar P, Mothes W, Uchil PD, Pazgier M, Finzi A. A Fc-enhanced NTD-binding non-neutralizing antibody delays virus spread and synergizes with a nAb to protect mice from lethal SARS-CoV-2 infection. Cell Reports 2022, 38: 110368. PMID: 35123652, PMCID: PMC8786652, DOI: 10.1016/j.celrep.2022.110368.
Structural basis and mode of action for two broadly neutralizing antibodies against SARS-CoV-2 emerging variants of concernLi W, Chen Y, Prévost J, Ullah I, Lu M, Gong SY, Tauzin A, Gasser R, Vézina D, Anand SP, Goyette G, Chaterjee D, Ding S, Tolbert WD, Grunst MW, Bo Y, Zhang S, Richard J, Zhou F, Huang RK, Esser L, Zeher A, Côté M, Kumar P, Sodroski J, Xia D, Uchil PD, Pazgier M, Finzi A, Mothes W. Structural basis and mode of action for two broadly neutralizing antibodies against SARS-CoV-2 emerging variants of concern. Cell Reports 2021, 38: 110210. PMID: 34971573, PMCID: PMC8673750, DOI: 10.1016/j.celrep.2021.110210.
A single dose of the SARS-CoV-2 vaccine BNT162b2 elicits Fc-mediated antibody effector functions and T cell responsesTauzin A, Nayrac M, Benlarbi M, Gong SY, Gasser R, Beaudoin-Bussières G, Brassard N, Laumaea A, Vézina D, Prévost J, Anand SP, Bourassa C, Gendron-Lepage G, Medjahed H, Goyette G, Niessl J, Tastet O, Gokool L, Morrisseau C, Arlotto P, Stamatatos L, McGuire AT, Larochelle C, Uchil P, Lu M, Mothes W, De Serres G, Moreira S, Roger M, Richard J, Martel-Laferrière V, Duerr R, Tremblay C, Kaufmann DE, Finzi A. A single dose of the SARS-CoV-2 vaccine BNT162b2 elicits Fc-mediated antibody effector functions and T cell responses. Cell Host & Microbe 2021, 29: 1137-1150.e6. PMID: 34133950, PMCID: PMC8175625, DOI: 10.1016/j.chom.2021.06.001.
Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus ParticlesLu M, Uchil P, Li W, Terry D, Gorman J, Zhang B, Zhou T, Ding S, Liu L, Ho D, Mascola J, Finzi A, Kwong P, Blanchard S, Mothes W. Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles. Biophysical Journal 2021, 120: 276a. PMCID: PMC7879775, DOI: 10.1016/j.bpj.2020.11.1757.
Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus ParticlesLu M, Uchil PD, Li W, Zheng D, Terry DS, Gorman J, Shi W, Zhang B, Zhou T, Ding S, Gasser R, Prévost J, Beaudoin-Bussières G, Anand SP, Laumaea A, Grover JR, Liu L, Ho DD, Mascola JR, Finzi A, Kwong PD, Blanchard SC, Mothes W. Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles. Cell Host & Microbe 2020, 28: 880-891.e8. PMID: 33242391, PMCID: PMC7664471, DOI: 10.1016/j.chom.2020.11.001.
Longitudinal bioluminescent imaging of HIV-1 infection during antiretroviral therapy and treatment interruption in humanized miceVentura JD, Beloor J, Allen E, Zhang T, Haugh KA, Uchil PD, Ochsenbauer C, Kieffer C, Kumar P, Hope TJ, Mothes W. Longitudinal bioluminescent imaging of HIV-1 infection during antiretroviral therapy and treatment interruption in humanized mice. PLOS Pathogens 2019, 15: e1008161. PMID: 31805155, PMCID: PMC6917343, DOI: 10.1371/journal.ppat.1008161.
Murine Leukemia Virus Exploits Innate Sensing by Toll-Like Receptor 7 in B-1 Cells To Establish Infection and Locally Spread in Mice.Pi R, Iwasaki A, Sewald X, Mothes W, Uchil PD. Murine Leukemia Virus Exploits Innate Sensing by Toll-Like Receptor 7 in B-1 Cells To Establish Infection and Locally Spread in Mice. Journal Of Virology 2019, 93 PMID: 31434732, PMCID: PMC6803250, DOI: 10.1128/jvi.00930-19.
Calcium Phosphate-Mediated Transfection of Adherent Cells or Cells Growing in Suspension: Variations on the Basic MethodKumar P, Nagarajan A, Uchil PD. Calcium Phosphate-Mediated Transfection of Adherent Cells or Cells Growing in Suspension: Variations on the Basic Method. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095455. PMID: 31575795, DOI: 10.1101/pdb.prot095455.
Transfection of Mammalian Cells with Calcium Phosphate-DNA Coprecipitates.Kumar P, Nagarajan A, Uchil PD. Transfection of Mammalian Cells with Calcium Phosphate-DNA Coprecipitates. Cold Spring Harbor Protocols 2019, 2019: pdb.top096255. PMID: 31575800, DOI: 10.1101/pdb.top096255.
Calcium Phosphate-Mediated Transfection of Cells with High-Molecular-Weight Genomic DNA.Kumar P, Nagarajan A, Uchil PD. Calcium Phosphate-Mediated Transfection of Cells with High-Molecular-Weight Genomic DNA. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095448. PMID: 31575794, DOI: 10.1101/pdb.prot095448.
Calcium Phosphate-Mediated Transfection of Eukaryotic Cells with Plasmid DNAs.Kumar P, Nagarajan A, Uchil PD. Calcium Phosphate-Mediated Transfection of Eukaryotic Cells with Plasmid DNAs. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095430. PMID: 31575793, DOI: 10.1101/pdb.prot095430.
Introducing Genes into Cultured Mammalian CellsKumar P, Nagarajan A, Uchil PD. Introducing Genes into Cultured Mammalian Cells. Cold Spring Harbor Protocols 2019, 2019: pdb.top095406. PMID: 31285274, DOI: 10.1101/pdb.top095406.
In vivo Imaging-Driven Approaches to Study Virus Dissemination and PathogenesisUchil PD, Haugh KA, Pi R, Mothes W. In vivo Imaging-Driven Approaches to Study Virus Dissemination and Pathogenesis. Annual Review Of Virology 2019, 6: 1-24. PMID: 31283440, PMCID: PMC7217087, DOI: 10.1146/annurev-virology-101416-041429.
DNA Transfection by Electroporation.Kumar P, Nagarajan A, Uchil PD. DNA Transfection by Electroporation. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095471. PMID: 31262956, DOI: 10.1101/pdb.prot095471.
ElectroporationKumar P, Nagarajan A, Uchil PD. Electroporation. Cold Spring Harbor Protocols 2019, 2019: pdb.top096271. PMID: 31262965, DOI: 10.1101/pdb.top096271.
Lipofection.Kumar P, Nagarajan A, Uchil PD. Lipofection. Cold Spring Harbor Protocols 2019, 2019: pdb.top096248. PMID: 30824627, DOI: 10.1101/pdb.top096248.
Histochemical Staining of Cell Monolayers for β-Galactosidase.Kumar P, Nagarajan A, Uchil PD. Histochemical Staining of Cell Monolayers for β-Galactosidase. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095422. PMID: 30824618, DOI: 10.1101/pdb.prot095422.
DNA Transfection Mediated by Cationic Lipid ReagentsKumar P, Nagarajan A, Uchil PD. DNA Transfection Mediated by Cationic Lipid Reagents. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095414. PMID: 30824617, DOI: 10.1101/pdb.prot095414.
Optical Transfection.Kumar P, Nagarajan A, Uchil PD. Optical Transfection. Cold Spring Harbor Protocols 2018, 2018: pdb.top096222. PMID: 30510131, DOI: 10.1101/pdb.top096222.
Selective Agents for Stable Transfection.Kumar P, Nagarajan A, Uchil PD. Selective Agents for Stable Transfection. Cold Spring Harbor Protocols 2018, 2018: pdb.top096230. PMID: 30181228, DOI: 10.1101/pdb.top096230.
A Biocontainment Procedure for Intravital Microscopy of High-Risk PathogensHaugh K, Pi R, Fontes B, Mothes W, Uchil P. A Biocontainment Procedure for Intravital Microscopy of High-Risk Pathogens. Applied Biosafety 2018, 23: 211-222. DOI: 10.1177/1535676018785177.
DEAE-Dextran Transfection.Kumar P, Nagarajan A, Uchil PD. DEAE-Dextran Transfection. Cold Spring Harbor Protocols 2018, 2018: pdb.top096263. PMID: 29967279, DOI: 10.1101/pdb.top096263.
Transfection Mediated by DEAE-Dextran.Kumar P, Nagarajan A, Uchil PD. Transfection Mediated by DEAE-Dextran. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095463. PMID: 29967275, DOI: 10.1101/pdb.prot095463.
Analysis of Cell Viability by the Lactate Dehydrogenase Assay.Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the Lactate Dehydrogenase Assay. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095497. PMID: 29858337, DOI: 10.1101/pdb.prot095497.
Analysis of Cell Viability by the MTT Assay.Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the MTT Assay. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095505. PMID: 29858338, DOI: 10.1101/pdb.prot095505.
Analysis of Cell Viability by the alamarBlue Assay.Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the alamarBlue Assay. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095489. PMID: 29858336, DOI: 10.1101/pdb.prot095489.
Small Interfering RNA-Mediated Control of Virus Replication in the CNS Is Therapeutic and Enables Natural Immunity to West Nile VirusBeloor J, Maes N, Ullah I, Uchil P, Jackson A, Fikrig E, Lee SK, Kumar P. Small Interfering RNA-Mediated Control of Virus Replication in the CNS Is Therapeutic and Enables Natural Immunity to West Nile Virus. Cell Host & Microbe 2018, 23: 549-556.e3. PMID: 29606496, PMCID: PMC6074029, DOI: 10.1016/j.chom.2018.03.001.
β-Galactosidase.Uchil PD, Nagarajan A, Kumar P. β-Galactosidase. Cold Spring Harbor Protocols 2017, 2017: pdb.top096198. PMID: 28974659, DOI: 10.1101/pdb.top096198.
Assay for β-Galactosidase in Extracts of Mammalian Cells.Uchil PD, Nagarajan A, Kumar P. Assay for β-Galactosidase in Extracts of Mammalian Cells. Cold Spring Harbor Protocols 2017, 2017: pdb.prot095778. PMID: 28974657, DOI: 10.1101/pdb.prot095778.
Proceedings of the Frontiers of Retrovirology Conference 2016Zurnic I, Hütter S, Lehmann U, Stanke N, Reh J, Kern T, Lindel F, Gerresheim G, Hamann M, Müllers E, Lesbats P, Cherepanov P, Serrao E, Engelman A, Lindemann D, Da Silva Santos C, Tartour K, Cimarelli A, Burdick R, Chen J, Sastri J, Hu W, Pathak V, Keppler O, Pradeau K, Eiler S, Levy N, Lennon S, Cianferani S, Emiliani S, Ruff M, Parissi V, Rato S, Rausell A, Munoz M, Telenti A, Ciuffi A, Zhyvoloup A, Melamed A, Anderson I, Planas D, Kriston-Vizi J, Ketteler R, Lee C, Merritt A, Ancuta P, Bangham C, Fassati A, Rodari A, Van Driessche B, Galais M, Delacourt N, Fauquenoy S, Vanhulle C, Kula A, Burny A, Rohr O, Van Lint C, van Montfort T, van der Sluis R, Speijer D, Berkhout B, Meng B, Rutkowski A, Berry N, Dölken L, Lever A, Schuster T, Asbach B, Wagner R, Gross C, Wiesmann V, Kalmer M, Wittenberg T, Gettemans J, Thoma-Kress A, Li M, Freed E, Liu S, Müller J, Münch J, Sewald X, Uchil P, Ladinsky M, Beloor J, Pi R, Herrmann C, Motamedi N, Murooka T, Brehm M, Greiner D, Mempel T, Bjorkman P, Kumar P, Mothes W, Joas S, Parrish E, Gnanadurai C, Lump E, Stürzel C, Parrish N, Sauermann U, Töpfer K, Schultheiss T, Bosinger S, Silvestri G, Apetrei C, Huot N, Müller-Trutwin M, Sauter D, Hahn B, Stahl-Hennig C, Kirchhoff F, Schumann G, Jung-Klawitter S, Fuchs N, Upton K, Muñoz-Lopez M, Shukla R, Wang J, Garcia-Canadas M, Lopez-Ruiz C, Gerhardt D, Sebe A, Grabundzija I, Gerdes P, Merkert S, Pulgarin A, Bock A, Held U, Witthuhn A, Haase A, Wolvetang E, Martin U, Ivics Z, Izsvák Z, Garcia-Perez J, Faulkner G, Hurst T, Katzourakis A, Magiorkinis G, Schott K, Derua R, Seifried J, Reuter A, Schmitz H, Tondera C, Brandariz-Nuñez A, Diaz-Griffero F, Janssens V, König R, Baldauf H, Stegmann L, Schwarz S, Trotard M, Martin M, Lenzi G, Burggraf M, Pan X, Fregoso O, Lim E, Abraham L, Erikson E, Nguyen L, Ambiel I, Rutsch F, Kim B, Emerman M, Fackler O, Wittmann S, Behrendt R, Volkmann B, Eissmann K, Gramberg T, Bolduan S, Koppensteiner H, Regensburg S, Brack-Werner R, Draenert R, Schindler M, Ducroux A, Xu S, Ponnurangam A, Franz S, Malassa A, Ewald E, Goffinet C, Fung S, Chan C, Yuen C, Kok K, Chan C, Jin D, Dittmer U, Kmiec D, Iyer S, Stürzel C, Hahn B, Ariumi Y, Yasuda-Inoue M, Kawano K, Tateishi S, Turelli P, Compton A, Roy N, Porrot F, Billet A, Casartelli N, Yount J, Liang C, Schwartz O, Magnus C, Reh L, Moore P, Uhr T, Weber J, Morris L, Trkola A, Grindberg R, Schlaepfer E, Schreiber G, Simon V, Speck R, Debyser Z, Vranckx L, Demeulemeester J, Saleh S, Verdin E, Cereseto A, Christ F, Gijsbers R, Wang G, Zhao N, Das A, Köstler J, Perdiguero B, Esteban M, Jacobs B, Montefiori D, LaBranche C, Yates N, Tomaras G, Ferrari G, Foulds K, Roederer M, Landucci G, Forthal D, Seaman M, Hawkins N, Self S, Phogat S, Tartaglia J, Barnett S, Burke B, Cristillo A, Ding S, Heeney J, Pantaleo G, Stab V, Ensser A, Tippler B, Burton D, Tenbusch M, Überla K, Alter G, Lofano G, Dugast A, Kulkarni V, Suscovich T, Opazo T, Barraza F, Herrera D, Garces A, Schwenke T, Tapia D, Cancino J, Arriagada G, Haußner C, Damm D, Rohrhofer A, Schmidt B, Eichler J, Midgley R, Wheeldon J, Piguet V, Khopkar P, Rohamare M, Kulkarni S, Godinho-Santos A, Hance A, Goncalves J, Mammano F, Gasser R, Hamoudi M, Pellicciotta M, Zhou Z, Visdeloup C, Colin P, Braibant M, Lagane B, Negroni M, Wamara J, Bannert N, Mesplede T, Osman N, Anstett K, Liang J, Pham H, Wainberg M, Shao W, Shan J, Kearney M, Wu X, Maldarelli F, Mellors J, Luke B, Coffin J, Hughes S, Fricke T, Opp S, Shepard C, Ivanov D, Valle-Casuso J, Kanja M, Cappy P, Negroni M, Lener D, Knyazhanskaya E, Anisenko A, Zatsepin T, Gottikh M, Komkov A, Minervina A, Nugmanov G, Nazarov V, Khodosevich K, Mamedov I, Lebedev Y, Colomer-Lluch M, Serra-Moreno R, Sarracino A, Gharu L, Pasternak A, Marcello A, McCartin A, Kulkarni A, Le Douce V, Gautier V, Baeyens A, Naessens E, Van Nuffel A, Weening K, Reilly A, Claeys E, Trypsteen W, Vandekerckhove L, Eyckerman S, Gevaert K, Verhasselt B, Mok H, Norton N, Fun A, Hirst J, Wills M, Miklik D, Senigl F, Hejnar J, Sakuragi J, Sakuragi S, Yokoyama M, Shioda T, Sato H, Bodem J, Moschall R, Denk S, Erkelenz S, Schenk C, Schaal H, Donhauser N, Socher E, Millen S, Sticht H, Gross C, Mann M, Wei G, Betts M, Liu Y, Kehl T, Russell R, Löchelt M, Hohn O, Mostafa S, Hanke K, Norley S, Chen C, Shingai M, Borrego P, Taveira N, Strebel K, Hellmund C, Meng B, Friedrich M, Hahn F, Setz C, Rauch P, Fraedrich K, Matthaei A, Henklein P, Traxdorf M, Fossen T, Schubert U, Khwaja A, Galilee M, Alian A, Schwalbe B, Hauser H, Schreiber M, Scherpenisse M, Cho Y, Kim J, Jeong D, Trejbalova K, Benesova M, Kucerova D, Vernerova Z, Amouroux R, Hajkova P, Elleder D, Hron T, Farkasova H, Padhi A, Paces J, Zhu H, Gifford R, Murcia P, Carrozza M, Niewiadomska A, Mazzei M, Abi-Said M, Hughes J, Hué S, Gifford R, Obasa A, Jacobs G, Engelbrecht S, Mack K, Starz K, Geyer M, Bibollet-Ruche F, Stürzel C, Leoz M, Plantier J, Argaw-Denboba A, Balestrieri E, Serafino A, Bucci I, Cipriani C, Spadafora C, Sinibaldi-Vallebona P, Matteucci C, Jayashree S, Neogi U, Chhangani A, Rathore S, Mathur B, Abati A, Koç B, Oğuzoğlu T, Shimauchi T, Caucheteux S, Turpin J, Finsterbusch K, Tokura Y, Souriant S, Balboa L, Pingris K, Kviatcowsky D, Raynaud-Messina B, Cougoule C, Mercier I, Kuroda M, González-Montaner P, Inwentarz S, Moraña E, del Carmen Sasiain M, Neyrolles O, Maridonneau-Parini I, Lugo-Villarino G, Vérollet C, Herrmann A, Thomas D, Bouzas N, Lahaye X, Bhargava A, Satoh T, Gentili M, Cerboni S, Silvin A, Conrad C, Ahmed-Belkacem H, Rodriguez E, Guichou J, Bosquet N, Piel M, Le Grand R, King M, Pawlotsky J, Manel N, Hofmann H, Vanwalscappel B, Bloch N, Landau N, Indik S, Hagen B, Valle-Casuso J, Allouch A, David A, Barré-Sinoussi F, Benkirane M, Pancino G, Saez-Cirion A, Lee W, Sloan R, Schulte B, Opp S, Blomberg J, Vargiu L, Rodriguez-Tomé P, Tramontano E, Sperber G, Kumari N, Ammosova T, Diaz S, Oneal P, Nekhai S, Fahrny A, Gers-Huber G, Audigé A, Jayaprakash A, Sachidanandam R, Hernandez M, Dillon-White M, Souriant S, Pingris K, Raynaud-Messina B, Cougoule C, Mercier I, Neyrolles O, Maridonneau-Parini I, Lugo-Villarino G, Maze E, Ham C, Almond N, Towers G, Belshaw R, de Sousa-Pereira P, Abrantes J, Pizzato M, Esteves P, Kahle T, Schmitt S, Merkel L, Reuter N, Stamminger T, Rosa I, Bishop K, Spinazzola A, Groom H, Vieyres G, Müsken M, Zillinger T, Hornung V, Barchet W, Häussler S, Pietschmann T, Javed A, Leuchte N, Salinas G, Opitz L, Sopper S, Mummert C, Hofmann C, Hückelhoven A, Bergmann S, Müller-Schmucker S, Harrer E, Dörrie J, Schaft N, Harrer T, Cardinaux L, Zahno M, Vogt H, Zanoni R, Bertoni G, Muenchhoff M, Goulder P, Keppler O, Rebensburg S, Helfer M, Zhang Y, Chen H, Bernier A, Gosselin A, Routy J, Wöhrl B, Schneider A, Corona A, Spöring I, Jordan M, Buchholz B, Maccioni E, Di Santo R, Schweimer K, Schölz C, Weinert B, Wagner S, Beli P, Miyake Y, Qi J, Jensen L, Streicher W, McCarthy A, Westwood N, Lain S, Cox J, Matthias P, Mann M, Bradner J, Choudhary C, Stern M, Valletta E, Frezza C, Marino-Merlo F, Grelli S, Serafino A, Mastino A, Macchi B, Kaulfuß M, Windmann S, Bayer W, Mikasi S, Jacobs G, Heß R, Bonsmann M, Kirschning C, Lepenies B, Kolenbrander A, Temchura V, Iijima K, Kobayashi J, Ishizaka Y. Proceedings of the Frontiers of Retrovirology Conference 2016. Retrovirology 2016, 13: 68. PMCID: PMC5046194, DOI: 10.1186/s12977-016-0294-5.
TRIM5 Retroviral Restriction Activity Correlates with the Ability To Induce Innate Immune SignalingLascano J, Uchil PD, Mothes W, Luban J. TRIM5 Retroviral Restriction Activity Correlates with the Ability To Induce Innate Immune Signaling. Journal Of Virology 2015, 90: 308-316. PMID: 26468522, PMCID: PMC4702541, DOI: 10.1128/jvi.02496-15.
HIV cell-to-cell transmission: effects on pathogenesis and antiretroviral therapyAgosto LM, Uchil PD, Mothes W. HIV cell-to-cell transmission: effects on pathogenesis and antiretroviral therapy. Trends In Microbiology 2015, 23: 289-295. PMID: 25766144, PMCID: PMC4417442, DOI: 10.1016/j.tim.2015.02.003.
Attachment of Cell-Binding Ligands to Arginine-Rich Cell-Penetrating Peptides Enables Cytosolic Translocation of Complexed siRNAZeller S, Choi CS, Uchil PD, Ban HS, Siefert A, Fahmy TM, Mothes W, Lee SK, Kumar P. Attachment of Cell-Binding Ligands to Arginine-Rich Cell-Penetrating Peptides Enables Cytosolic Translocation of Complexed siRNA. Cell Chemical Biology 2014, 22: 50-62. PMID: 25544044, PMCID: PMC4320807, DOI: 10.1016/j.chembiol.2014.11.009.
TRIM15 is a focal adhesion protein that regulates focal adhesion disassemblyUchil P, Pawliczek T, Reynolds T, Ding S, Hinz A, Munro J, Huang F, Floyd R, Yang H, Hamilton W, Bewersdorf J, Xiong Y, Calderwood D, Mothes W. TRIM15 is a focal adhesion protein that regulates focal adhesion disassembly. Development 2014, 141: e1906-e1906. DOI: 10.1242/dev.117242.
Human Genome-Wide RNAi Screen Identifies an Essential Role for Inositol Pyrophosphates in Type-I Interferon ResponsePulloor NK, Nair S, McCaffrey K, Kostic AD, Bist P, Weaver JD, Riley AM, Tyagi R, Uchil PD, York JD, Snyder SH, García-Sastre A, Potter BV, Lin R, Shears SB, Xavier RJ, Krishnan MN. Human Genome-Wide RNAi Screen Identifies an Essential Role for Inositol Pyrophosphates in Type-I Interferon Response. PLOS Pathogens 2014, 10: e1003981. PMID: 24586175, PMCID: PMC3937324, DOI: 10.1371/journal.ppat.1003981.
Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithmsHuang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, Bewersdorf J. Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms. Nature Methods 2013, 10: 653-658. PMID: 23708387, PMCID: PMC3696415, DOI: 10.1038/nmeth.2488.
Cell-to-Cell Transmission Can Overcome Multiple Donor and Target Cell Barriers Imposed on Cell-Free HIVZhong P, Agosto LM, Ilinskaya A, Dorjbal B, Truong R, Derse D, Uchil PD, Heidecker G, Mothes W. Cell-to-Cell Transmission Can Overcome Multiple Donor and Target Cell Barriers Imposed on Cell-Free HIV. PLOS ONE 2013, 8: e53138. PMID: 23308151, PMCID: PMC3538641, DOI: 10.1371/journal.pone.0053138.
TRIM Protein-Mediated Regulation of Inflammatory and Innate Immune Signaling and Its Association with Antiretroviral ActivityUchil PD, Hinz A, Siegel S, Coenen-Stass A, Pertel T, Luban J, Mothes W. TRIM Protein-Mediated Regulation of Inflammatory and Innate Immune Signaling and Its Association with Antiretroviral Activity. Journal Of Virology 2012, 87: 257-272. PMID: 23077300, PMCID: PMC3536418, DOI: 10.1128/jvi.01804-12.
Targeted Disruption of the CCR5 Gene in Human Hematopoietic Stem Cells Stimulated by Peptide Nucleic AcidsSchleifman EB, Bindra R, Leif J, del Campo J, Rogers FA, Uchil P, Kutsch O, Shultz LD, Kumar P, Greiner DL, Glazer PM. Targeted Disruption of the CCR5 Gene in Human Hematopoietic Stem Cells Stimulated by Peptide Nucleic Acids. Cell Chemical Biology 2011, 18: 1189-1198. PMID: 21944757, PMCID: PMC3183429, DOI: 10.1016/j.chembiol.2011.07.010.
TRIM22 Inhibits HIV-1 Transcription Independently of Its E3 Ubiquitin Ligase Activity, Tat, and NF-κB-Responsive Long Terminal Repeat ElementsKajaste-Rudnitski A, Marelli SS, Pultrone C, Pertel T, Uchil PD, Mechti N, Mothes W, Poli G, Luban J, Vicenzi E. TRIM22 Inhibits HIV-1 Transcription Independently of Its E3 Ubiquitin Ligase Activity, Tat, and NF-κB-Responsive Long Terminal Repeat Elements. Journal Of Virology 2011, 85: 5183-5196. PMID: 21345949, PMCID: PMC3126207, DOI: 10.1128/jvi.02302-10.
Anaplasma phagocytophilum AptA modulates Erk1/2 signallingSukumaran B, Mastronunzio JE, Narasimhan S, Fankhauser S, Uchil PD, Levy R, Graham M, Colpitts TM, Lesser CF, Fikrig E. Anaplasma phagocytophilum AptA modulates Erk1/2 signalling. Cellular Microbiology 2010, 13: 47-61. PMID: 20716207, PMCID: PMC3005019, DOI: 10.1111/j.1462-5822.2010.01516.x.
TRIM5alpha contributes to the anti-viral statePertel T, Neagu M, Hausman S, Zufferey M, Uchil P, Mothes W, Luban J. TRIM5alpha contributes to the anti-viral state. Retrovirology 2009, 6: o6. PMCID: PMC2766986, DOI: 10.1186/1742-4690-6-s2-o6.
HIV Entry RevisitedUchil PD, Mothes W. HIV Entry Revisited. Cell 2009, 137: 402-404. PMID: 19410537, PMCID: PMC2803685, DOI: 10.1016/j.cell.2009.04.033.
Human TRIM Gene Expression in Response to InterferonsCarthagena L, Bergamaschi A, Luna JM, David A, Uchil PD, Margottin-Goguet F, Mothes W, Hazan U, Transy C, Pancino G, Nisole S. Human TRIM Gene Expression in Response to Interferons. PLOS ONE 2009, 4: e4894. PMID: 19290053, PMCID: PMC2654144, DOI: 10.1371/journal.pone.0004894.
Retroviruses Human Immunodeficiency Virus and Murine Leukemia Virus Are Enriched in Phosphoinositides▿ †Chan R, Uchil PD, Jin J, Shui G, Ott DE, Mothes W, Wenk MR. Retroviruses Human Immunodeficiency Virus and Murine Leukemia Virus Are Enriched in Phosphoinositides▿ †. Journal Of Virology 2008, 82: 11228-11238. PMID: 18799574, PMCID: PMC2573248, DOI: 10.1128/jvi.00981-08.
RNA interference screen for human genes associated with West Nile virus infectionKrishnan MN, Ng A, Sukumaran B, Gilfoy FD, Uchil PD, Sultana H, Brass AL, Adametz R, Tsui M, Qian F, Montgomery RR, Lev S, Mason PW, Koski RA, Elledge SJ, Xavier RJ, Agaisse H, Fikrig E. RNA interference screen for human genes associated with West Nile virus infection. Nature 2008, 455: 242-245. PMID: 18690214, PMCID: PMC3136529, DOI: 10.1038/nature07207.
Murine Leukemia Virus Spreading in Mice Impaired in the Biogenesis of Secretory Lysosomes and Ca2+-Regulated ExocytosisChan WT, Sherer NM, Uchil PD, Novak EK, Swank RT, Mothes W. Murine Leukemia Virus Spreading in Mice Impaired in the Biogenesis of Secretory Lysosomes and Ca2+-Regulated Exocytosis. PLOS ONE 2008, 3: e2713. PMID: 18629000, PMCID: PMC2443282, DOI: 10.1371/journal.pone.0002713.
Effective suppression of HIV-1 by artificial bispecific miRNA targeting conserved sequences with tolerance for wobble base-pairingSon J, Uchil PD, Kim YB, Shankar P, Kumar P, Lee SK. Effective suppression of HIV-1 by artificial bispecific miRNA targeting conserved sequences with tolerance for wobble base-pairing. Biochemical And Biophysical Research Communications 2008, 374: 214-218. PMID: 18619945, DOI: 10.1016/j.bbrc.2008.06.125.
Organization of Flaviviral Replicase Proteins in Virus‐Induced Membranes: a Role for NS1′ in Japanese Encephalitis Virus RNA SynthesisSatchidanandam V, Uchil PD, Kumar P. Organization of Flaviviral Replicase Proteins in Virus‐Induced Membranes: a Role for NS1′ in Japanese Encephalitis Virus RNA Synthesis. 2006, 277: 136-148. PMID: 17319159, DOI: 10.1002/0470058005.ch10.
Nuclear Localization of Flavivirus RNA Synthesis in Infected CellsUchil P, Kumar A, Satchidanandam V. Nuclear Localization of Flavivirus RNA Synthesis in Infected Cells. Journal Of Virology 2006, 80: 5451-5464. PMID: 16699025, PMCID: PMC1472159, DOI: 10.1128/jvi.01982-05.
Viral entry: a detour through multivesicular bodiesUchil P, Mothes W. Viral entry: a detour through multivesicular bodies. Nature Cell Biology 2005, 7: 641-642. PMID: 15990891, DOI: 10.1038/ncb0705-641.
Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparationsKumar P, Uchil PD, Sulochana P, Nirmala G, Chandrashekar R, Haridattatreya M, Satchidanandam V. Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations. Archives Of Virology 2003, 148: 1569-1591. PMID: 12898332, DOI: 10.1007/s00705-003-0118-5.
Characterization of RNA synthesis, replication mechanism, and in vitro RNA-dependent RNA polymerase activity of japanese encephalitis virusUchil P, Satchidanandam V. Characterization of RNA synthesis, replication mechanism, and in vitro RNA-dependent RNA polymerase activity of japanese encephalitis virus. Virology 2003, 307: 358-371. PMID: 12667804, DOI: 10.1016/s0042-6822(02)00130-7.
The regulatory elements of the Mycobacterium tuberculosis gene Rv3881c function efficiently in Escherichia coliSatchidanandam V, Amara R, Uchil P, Singh V. The regulatory elements of the Mycobacterium tuberculosis gene Rv3881c function efficiently in Escherichia coli. FEMS Microbiology Letters 2003, 218: 365-370. PMID: 12586418, DOI: 10.1016/s0378-1097(02)01185-0.
Determination of Host Specificity of Cowpea Miscellany Rhizobium spp. by nodABC-lacZ FusionPandya S, Uchil P, Subramanian M, Desai A. Determination of Host Specificity of Cowpea Miscellany Rhizobium spp. by nodABC-lacZ Fusion. Current Microbiology 1998, 36: 361-364. PMID: 9608748, DOI: 10.1007/s002849900323.

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