Research & Publications
Visualizing 3D structure and dynamics at the molecular scale is a current and critical need in biomedical research. Many sub-cellular features, for example the morphology of many organelles or the 3D organization of chromatin, cannot be resolved by standard light microscopy. Improving the resolution of light microscopy has therefore been an urgent need of biological research for many decades. Today, several fluorescence microscopy methods achieve resolutions about 10 times better than standard light microscopes. Our research group in the Department of Cell Biology at Yale University School of Medicine is developing a range of techniques with spatial and/or temporal resolutions going far beyond current technology. Additionally, our research group applies these techniques to a diverse set of biological questions.
Specialized Terms: Super-resolution fluorescence microscopy
Extensive Research Description
Our laboratory develops super-resolution Stimulated Emission Depletion (STED) microscopy, Single-molecule Localization Microscopy (FPALM/PALM/STORM/DNA-PAINT, etc.) and pan-Expansion Microscopy (pan-ExM) techniques. We are actively developing these methods to improve the capabilities (speed, 3D resolution, robustness, multicolor labeling) of these imaging techniques and thereby expand the application range and impact of super-resolution microscopy. In collaboration with a diverse set of research groups at Yale University and outside, we apply our new techniques to current biomedical questions, in particular related to the endoplasmic reticulum, the Golgi complex, and the cell nucleus.
Cell Nucleus; Endoplasmic Reticulum; Microscopy, Fluorescence; Microscopy, Confocal; Cellular Structures