Lynn Cooley, PhD
Research & Publications
We are interested in the cellular mechanisms that underlie polarity and cell growth during development. Our research is focused on understanding how maternal components are made and delivered to oocytes during Drosophila oogenesis. Using mutants with incomplete oocyte growth, we have discovered key roles for the actin cytoskeleton. For example, the ring canals connecting growing oocytes with their nurse cells are stabilized by a special population of bundled actin filaments.
The dramatic growth of ring canals during oogenesis requires both actin polymerization and depolymerization, making ring canals a valuable model for in vivo actin dynamics. The polarized movement of maternal mRNA and protein through ring canals from nurse cells to the oocyte is highly regulated. We identified proteins specifically targeted to the oocyte by GFP protein trapping; we are determining the mechanism of targeting using both live imaging and molecular dissection of the proteins to identify localization signals.
Specialized Terms: Molecular Genetics of Drosophila Oogenesis; Actin Cytoskeleton Regulation; Drosophila; Oogenesis; Ring Canal; Ovarian Muscle Function
Extensive Research Description
Early germline development in animals, including flies, relies on a non-canonical form of mitosis. Daughters of germline stem cells undergo a tightly controlled number of mitotic cell divisions with incomplete cytokinesis so that bridges of cytoplasm remain to connect clusters of sister cells. These residual connections are transformed into stable intercellular junctions called ring canals, which are needed for oocyte growth.
In females, this transformation involves recruiting a highly dynamic actin cytoskeleton and many associated actin-binding proteins. Using a variety of genetic and molecular approaches, we have identified many ring canal proteins, and we are actively working toward characterizing their functions. We are also studying the role of ring canals in the polarized transport of maternal mRNAs, proteins and organelles from nurse cells and to the oocyte.
While ring canals are ubiquitous in germline cells, their presence and function in somatic cells are largely unexplored. In order to understand how these fascinating structures contribute to the biology of non-germline cells, we are characterizing somatic ring canals in epithelial cells of the Drosophila ovary and imaginal discs using cell biology and genetics.
Recently we discovered a novel muscle type in the Drosophila ovary that contains striated sarcomeres, but only a single nucleus. This indicates the muscles did not form by typical myoblast fusion. Importantly, the presence of one nucleus means we can use powerful genetic clonal analysis to analyze the effects of mutations affecting muscle proteins, including those associated with human musclular dystrophy. In addition, we can study proliferation of these muscles in adults and the pool of progenitor stem cells that supply new muscle cells in adults.
Biology; Cell Biology; Drosophila; Genetics; Actin Cytoskeleton; Molecular Biology; Oogenesis
- Corrigendum to: “Somatic insulin signaling regulates a germline starvation response in Drosophila egg chambers” [Dev. Biol. 398 (2) (2015) 206–217]Burn K, Shimada Y, Ayers K, Lu F, Hudson A, Cooley L. Corrigendum to: “Somatic insulin signaling regulates a germline starvation response in Drosophila egg chambers” [Dev. Biol. 398 (2) (2015) 206–217] Developmental Biology 2015, 405: 340. DOI: 10.1016/j.ydbio.2015.07.016.
- Somatic insulin signaling regulates a germline starvation response in Drosophila egg chambersBurn KM, Shimada Y, Ayers K, Vemuganti S, Lu F, Hudson A, Cooley L. Somatic insulin signaling regulates a germline starvation response in Drosophila egg chambers Developmental Biology 2014, 398: 206-217. PMID: 25481758, PMCID: PMC4340711, DOI: 10.1016/j.ydbio.2014.11.021.
- Antivirulence Properties of an Antifreeze ProteinHeisig M, Abraham N, Liu L, Neelakanta G, Mattessich S, Sultana H, Shang Z, Ansari J, Killiam C, Walker W, Cooley L, Flavell R, Agaisse H, Fikrig E. Antivirulence Properties of an Antifreeze Protein Cell Reports 2014, 9: 2344. DOI: 10.1016/j.celrep.2014.12.003.
- Intercellular protein movement in syncytial Drosophila follicle cellsAiroldi S, McLean P, Shimada Y, Cooley L. Intercellular protein movement in syncytial Drosophila follicle cells Development 2012, 139: e207-e207. DOI: 10.1242/dev.077784.
- Exploring Strategies for Protein Trapping in DrosophilaQuiñones-Coello A, Petrella LN, Ayers K, Melillo A, Mazzalupo S, Hudson AM, Wang S, Castiblanco C, Buszczak M, Hoskins RA, Cooley L. Exploring Strategies for Protein Trapping in Drosophila Genetics 2007, 175: 1089-1104. PMID: 17179094, PMCID: PMC1840052, DOI: 10.1534/genetics.106.065995.
- Comparative Aspects of Animal OogenesisMatova N, Cooley L. Comparative Aspects of Animal Oogenesis Developmental Biology 2001, 231: 291-320. PMID: 11237461, DOI: 10.1006/dbio.2000.0120.
- Filamins as integrators of cell mechanics and signallingStossel T, Condeelis J, Cooley L, Hartwig J, Noegel A, Schleicher M, Shapiro S. Filamins as integrators of cell mechanics and signalling Nature Reviews Molecular Cell Biology 2001, 2: 138-145. PMID: 11252955, DOI: 10.1038/35052082.
- The kelch repeat superfamily of proteins: propellers of cell functionAdams J, Kelso R, Cooley L, Adams J, Kelso R, Cooley L. The kelch repeat superfamily of proteins: propellers of cell function Trends In Cell Biology 2000, 10: 17-24. PMID: 10603472, DOI: 10.1016/s0962-8924(99)01673-6.
- Drosophila Filamin encoded by the cheerio locus is a component of ovarian ring canalsSokol N, Cooley L. Drosophila Filamin encoded by the cheerio locus is a component of ovarian ring canals Current Biology 1999, 9: 1221-1230. PMID: 10556087, DOI: 10.1016/s0960-9822(99)80502-8.
- Drosophila Ring Canal Growth Requires Src and Tec KinasesCooley L. Drosophila Ring Canal Growth Requires Src and Tec Kinases Cell 1998, 93: 913-915. PMID: 9635420, DOI: 10.1016/s0092-8674(00)81196-4.
- GENETIC ANALYSIS OF THE ACTIN CYTOSKELETON IN THE DROSOPHILA OVARYRobinson D, Cooley L. GENETIC ANALYSIS OF THE ACTIN CYTOSKELETON IN THE DROSOPHILA OVARY Annual Review Of Cell And Developmental Biology 1997, 13: 147-170. PMID: 9442871, DOI: 10.1146/annurev.cellbio.13.1.147.
- Drosophila Kelch Is an Oligomeric Ring Canal Actin OrganizerRobinson D, Cooley L. Drosophila Kelch Is an Oligomeric Ring Canal Actin Organizer Journal Of Cell Biology 1997, 138: 799-810. PMID: 9265647, PMCID: PMC2138045, DOI: 10.1083/jcb.138.4.799.
- Formation of the Drosophila ovarian ring canal inner rim depends on cheerio.Robinson D, Smith-Leiker T, Sokol N, Hudson A, Cooley L. Formation of the Drosophila ovarian ring canal inner rim depends on cheerio. Genetics 1997, 145: 1063-72. PMID: 9093858, PMCID: PMC1207876, DOI: 10.1093/genetics/145.4.1063.
- Using explicitly represented biological relationships for database navigation and searching via the World-Wide WebPanzer S, Cooley L, Miller P. Using explicitly represented biological relationships for database navigation and searching via the World-Wide Web Bioinformatics 1997, 13: 281-290. PMID: 9183533, DOI: 10.1093/bioinformatics/13.3.281.
- Stable intercellular bridges in development: the cytoskeleton lining the tunnelRobinson D, Cooley L. Stable intercellular bridges in development: the cytoskeleton lining the tunnel Trends In Cell Biology 1996, 6: 474-479. PMID: 15157506, DOI: 10.1016/0962-8924(96)84945-2.
- Oogenesis: Variations on a themeCooley L. Oogenesis: Variations on a theme Genesis 1995, 16: 1-5. PMID: 7758241, DOI: 10.1002/dvg.1020160103.
- Cytoskeletal Functions During Drosophila OogenesisCooley L, Theurkauf W. Cytoskeletal Functions During Drosophila Oogenesis Science 1994, 266: 590-596. PMID: 7939713, DOI: 10.1126/science.7939713.
- Intercellular Cytoplasm Transport during Drosophila OogenesisMahajan-Miklos S, Cooley L. Intercellular Cytoplasm Transport during Drosophila Oogenesis Developmental Biology 1994, 165: 336-351. PMID: 7958404, DOI: 10.1006/dbio.1994.1257.
- The villin-like protein encoded by the Drosophila quail gene is required for actin bundle assembly during oogenesisMahajan-Miklos S, Cooley L. The villin-like protein encoded by the Drosophila quail gene is required for actin bundle assembly during oogenesis Cell 1994, 78: 291-301. PMID: 8044841, DOI: 10.1016/0092-8674(94)90298-4.
- The specialized cytoskeleton of theDrosophila egg chamberKnowles B, Cooley L. The specialized cytoskeleton of theDrosophila egg chamber Trends In Genetics 1994, 10: 235-241. PMID: 8091503, DOI: 10.1016/0168-9525(94)90170-8.
- Chapter 28 Looking at OogenesisVerheyen E, Cooley L. Chapter 28 Looking at Oogenesis 1994, 44: 545-561. PMID: 7707970, DOI: 10.1016/s0091-679x(08)60931-0.
- Kelch encodes a component of intercellular bridges in Drosophila egg chambersXue F, Cooley L. Kelch encodes a component of intercellular bridges in Drosophila egg chambers Cell 1993, 72: 681-693. PMID: 8453663, DOI: 10.1016/0092-8674(93)90397-9.
- chickadee encodes a profilin required for intercellular cytoplasm transport during Drosophila oogenesisCooley L, Verheyen E, Ayers K. chickadee encodes a profilin required for intercellular cytoplasm transport during Drosophila oogenesis Cell 1992, 69: 173-184. PMID: 1339308, DOI: 10.1016/0092-8674(92)90128-y.