Jorge Galán, PhD, DVM
Research & Publications
Biography
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Research Summary
Our laboratory studies the pathogenesis of two intestinal pathogens, Salmonella enterica and Campylobacter jejuni. Combined, these two pathogens account for the majority of cases of infectious diarrhea world-wide leading to an estimated 2,000,000 deaths. We take a multidisciplinary approach in our studies involving bacterial genetics, biochemistry, cell biology, immunology as well as structural biology. As a result, we are beginning to define not only the molecular details of the host pathogen interactions but also the atomic interface between these pathogens and the host.
Extensive Research Description
Microbial pathogens have evolved unique ways to interact with their hosts. In many instances the terms of this interaction reflect the co-evolutionary balance that the host and pathogen must reach in order to secure
their survival. It is therefore not surprising that bacterial pathogens have evolved a large array of virulence
factors well suited to interfere with or stimulate a variety of host-cell responses in order to invade, survive and replicate within their hosts. The identification and characterization of these virulence factors is proving to be a fruitful area of research in more ways than expected.
The understanding of how pathogens interact with their hosts is not only providing the basis for the development of novel therapeutic approaches but also a number of very sophisticated tools for probing basic aspects of cellular physiology and immunology. Our laboratory studies the pathogenesis of two intestinal pathogens, Salmonella enterica and Campylobacter jejuni. Combined, these two pathogens account for the vast majority of cases of infectious diarrhea world-wide leading to an estimated 2,000,000 deads. We are interested in characterizing the bacterial determinants involved in these interactions as well as the cell biology and immunobiology of this process.
We take a multidisciplinary approach in our studies involving bacterial genetics, biochemistry, cell biology, immunology as well as structural biology. As a result, we are beginning to define not only the molecular details of the host pathogen interactions but also the atomic interphase between these pathogens and the host. Our laboratory has also an interest in vaccine development that stems from our discovery of a specialized organelle in Salmonella enterica (the “type III secretion system”) that mediates the transfer of bacterial proteins into host cells. We have harnessed this system for the delivery of heterologous proteins as a means to delivery antigens to the Class I and Class II antigen presenting pathways by avirulent strains of Salmonella.
Specific areas of interest include:
- The study of type III protein secretion machines, specialized bacterial organelles whose function is to delivery bacterial proteins into eukaryotic host cells. We are interested in understanding the mechanism of action of this multi-protein machine, as well as to understand the activities of the effector proteins delivered by this machine. We carried out these studies in Salmonella enterica but it is expected that knowledge gained from these studies may help understand the pathogenesis of many other bacteria since this system is conserved and widespread among several important pathogens.
- The harnessing of the protein-delivery capability of the type III secretion machine for the development of therapeutic approaches, including vaccines.
- The development of strategies that target the activities of type III secretion systems with the ultimate goal of developing new therapeutics to combat diseases caused by pathogens that encode these protein-delivery machines.
- The study the mechanism of pathogenesis of Salmonella typhi, which causes typhoid fever in humans, a life-threatening disease that causes more than 200,000 deaths world-wide. We focus our efforts on the study of "typhoid toxin", a toxin exclusively produced by Salmonella typhi that we recently identified and it is central for the pathogenesis of typhoid fever.
- The study of the mechanisms by which Campylobacter jejuni is able to colonize the gut and the role of the intestinal microbiota in both, facilitating and preventing colonization.
- The investigation of the in vivo metabolism of C. jejuni. We are specifically interested in identifying its carbon sources and respiration substrates during infection and the contribution of the intestinal microbiota in providing those nutrients.
Coauthors
Research Interests
Campylobacter; Cell Biology; Immunity, Innate; Inflammation; Microbiology; Microscopy; Microscopy, Fluorescence; Salmonella; Cryoelectron Microscopy; Host-Pathogen Interactions; Microbiota; Analytical, Diagnostic and Therapeutic Techniques and Equipment; Type III Secretion Systems
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Selected Publications
- Assembly and architecture of the type III secretion sorting platformSoto J, Galán J, Lara-Tejero M. Assembly and architecture of the type III secretion sorting platform Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2218010119. PMID: 36512499, PMCID: PMC9907115, DOI: 10.1073/pnas.2218010119.
- Typhoid toxin sorting and exocytic transport from Salmonella Typhi-infected cellsChang SJ, Hsu YT, Chen Y, Lin YY, Lara-Tejero M, Galan JE. Typhoid toxin sorting and exocytic transport from Salmonella Typhi-infected cells ELife 2022, 11: e78561. PMID: 35579416, PMCID: PMC9142146, DOI: 10.7554/elife.78561.
- The Injectisome, a Complex Nanomachine for Protein Injection into Mammalian CellsLara-tejero M, Galán J. The Injectisome, a Complex Nanomachine for Protein Injection into Mammalian Cells 2019, 245-259. DOI: 10.1128/9781683670285.ch20.
- Antibacterial Flavonoids from Medicinal Plants Covalently Inactivate Type III Protein Secretion SubstratesTsou LK, Lara-Tejero M, RoseFigura J, Zhang ZJ, Wang YC, Yount JS, Lefebre M, Dossa PD, Kato J, Guan F, Lam W, Cheng YC, Galán J, Hang HC. Antibacterial Flavonoids from Medicinal Plants Covalently Inactivate Type III Protein Secretion Substrates Journal Of The American Chemical Society 2016, 138: 2209-2218. PMID: 26847396, PMCID: PMC4831573, DOI: 10.1021/jacs.5b11575.
- 9 Typhoid toxinGalan J. 9 Typhoid toxin 2015, 261-266. DOI: 10.1016/b978-0-12-800188-2.00009-4.
- Interaction of Campylobacter jejuni with Host CellsWatson R, Galán J. Interaction of Campylobacter jejuni with Host Cells 2008, 287-296. DOI: 10.1128/9781555815554.ch16.
- Modulation of the actin cytoskeleton by SalmonellaGalan J. Modulation of the actin cytoskeleton by Salmonella The FASEB Journal 2008, 22: 530.1-530.1. DOI: 10.1096/fasebj.22.1_supplement.530.1.
- Structure, assembly and function of the Salmonella type III protein secretion organelleGalán J. Structure, assembly and function of the Salmonella type III protein secretion organelle 2007, 2007 DOI: 10.1240/sav_gbm_2007_h_002101.
- The Salmonella thyphimurium type III protein secretion system: An effective antigen delivery platform for cancer therapeuticsGalán J. The Salmonella thyphimurium type III protein secretion system: An effective antigen delivery platform for cancer therapeutics Drugs Of The Future 2007, 32: 985. DOI: 10.1358/dof.2007.032.11.1132406.
- Unique Features of a Highly Pathogenic Campylobacter jejuni StrainHofreuter D, Tsai J, Watson R, Novik V, Altman B, Benitez M, Clark C, Perbost C, Jarvie T, Du L, Galán J. Unique Features of a Highly Pathogenic Campylobacter jejuni Strain Infection And Immunity 2006, 75: 542-542. PMCID: PMC1828408, DOI: 10.1128/iai.01701-06.
- Differential activation and function of Rho GTPases during Salmonella– host cell interactionsPatel J, Galán J. Differential activation and function of Rho GTPases during Salmonella– host cell interactions Journal Of Experimental Medicine 2006, 203: i30-i30. DOI: 10.1084/jem20312oia30.
- Differential activation and function of Rho GTPases during Salmonella–host cell interactionsPatel JC, Galán J. Differential activation and function of Rho GTPases during Salmonella–host cell interactions Journal Of Cell Biology 2006, 175: 453-463. PMID: 17074883, PMCID: PMC2064522, DOI: 10.1083/jcb.200605144.
- Role of the caspase-1 inflammasome in Salmonella typhimurium pathogenesisLara-Tejero M, Sutterwala FS, Ogura Y, Grant EP, Bertin J, Coyle AJ, Flavell RA, Galán J. Role of the caspase-1 inflammasome in Salmonella typhimurium pathogenesis Journal Of Experimental Medicine 2006, 203: 1407-1412. PMID: 16717117, PMCID: PMC2118315, DOI: 10.1084/jem.20060206.
- Structural Insights into the Assembly of the Type III Secretion Needle ComplexMarlovits TC, Kubori T, Sukhan A, Thomas DR, Galán J, Unger VM. Structural Insights into the Assembly of the Type III Secretion Needle Complex Science 2004, 306: 1040-1042. PMID: 15528446, PMCID: PMC1459965, DOI: 10.1126/science.1102610.
- Salmonella Modulates Vesicular Traffic by Altering Phosphoinositide MetabolismHernandez LD, Hueffer K, Wenk MR, Galán J. Salmonella Modulates Vesicular Traffic by Altering Phosphoinositide Metabolism Science 2004, 304: 1805-1807. PMID: 15205533, DOI: 10.1126/science.1098188.
- Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequencesMcKinney JS, Zhang H, Kubori T, Galán J, Altman S. Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequences Nucleic Acids Research 2004, 32: 848-854. PMID: 14762212, PMCID: PMC373343, DOI: 10.1093/nar/gkh219.
- A Salmonella protein causes macrophage cell death by inducing autophagyHernandez LD, Pypaert M, Flavell RA, Galán J. A Salmonella protein causes macrophage cell death by inducing autophagy Journal Of Cell Biology 2003, 163: 1123-1131. PMID: 14662750, PMCID: PMC2173598, DOI: 10.1083/jcb.200309161.
- Role of tyrosine kinases and the tyrosine phosphatase SptP in the interaction of Salmonella with host cellsMurli S, Watson R, Galán J. Role of tyrosine kinases and the tyrosine phosphatase SptP in the interaction of Salmonella with host cells Cellular Microbiology 2001, 3: 795-810. PMID: 11736992, DOI: 10.1046/j.1462-5822.2001.00158.x.
- Induction of specific CD8+ memory T cells and long lasting protection following immunization with Salmonella typhimurium expressing a lymphocytic choriomeningitis MHC class I-restricted epitopeShams H, Poblete F, Rüssmann H, Galán J, Donis R. Induction of specific CD8+ memory T cells and long lasting protection following immunization with Salmonella typhimurium expressing a lymphocytic choriomeningitis MHC class I-restricted epitope Vaccine 2001, 20: 577-585. PMID: 11672924, DOI: 10.1016/s0264-410x(01)00363-2.
- SALMONELLA INTERACTIONS WITH HOST CELLS: Type III Secretion at WorkGalán J. SALMONELLA INTERACTIONS WITH HOST CELLS: Type III Secretion at Work Annual Review Of Cell And Developmental Biology 2001, 17: 53-86. PMID: 11687484, DOI: 10.1146/annurev.cellbio.17.1.53.
- Maintenance of an unfolded polypeptide by a cognate chaperone in bacterial type III secretionStebbins C, Galán J. Maintenance of an unfolded polypeptide by a cognate chaperone in bacterial type III secretion Nature 2001, 414: 77-81. PMID: 11689946, DOI: 10.1038/35102073.
- Salmonella entry into host cells: the work in concert of type III secreted effector proteinsZhou D, Galán J. Salmonella entry into host cells: the work in concert of type III secreted effector proteins Microbes And Infection 2001, 3: 1293-1298. PMID: 11755417, DOI: 10.1016/s1286-4579(01)01489-7.
- Structural mimicry in bacterial virulenceStebbins C, Galán J. Structural mimicry in bacterial virulence Nature 2001, 412: 701-705. PMID: 11507631, DOI: 10.1038/35089000.
- CdtA, CdtB, and CdtC Form a Tripartite Complex That Is Required for Cytolethal Distending Toxin ActivityLara-Tejero M, Galán J. CdtA, CdtB, and CdtC Form a Tripartite Complex That Is Required for Cytolethal Distending Toxin Activity Infection And Immunity 2001, 69: 4358-4365. PMID: 11401974, PMCID: PMC98507, DOI: 10.1128/iai.69.7.4358-4365.2001.
- Common and Contrasting Themes of Plant and Animal DiseasesStaskawicz B, Mudgett M, Dangl J, Galan J. Common and Contrasting Themes of Plant and Animal Diseases Science 2001, 292: 2285-2289. PMID: 11423652, DOI: 10.1126/science.1062013.
- A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalizationZhou D, Chen L, Hernandez L, Shears S, Galán J. A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization Molecular Microbiology 2001, 40: 1461-1461. DOI: 10.1046/j.1365-2958.2001.02423.x.
- In Vitro Transposition System for Efficient Generation of Random Mutants of Campylobacter jejuniColegio O, Griffin T, Grindley N, Galán J. In Vitro Transposition System for Efficient Generation of Random Mutants of Campylobacter jejuni Journal Of Bacteriology 2001, 183: 2384-2388. PMID: 11244083, PMCID: PMC95150, DOI: 10.1128/jb.183.7.2384-2388.2001.
- Characterization of the mutS‐proximal region of the Salmonella typhimurium SPI‐1 identifies a group of pathogenicity island‐associated genesPancetti A, Galán J. Characterization of the mutS‐proximal region of the Salmonella typhimurium SPI‐1 identifies a group of pathogenicity island‐associated genes FEMS Microbiology Letters 2001, 197: 203-208. PMID: 11313135, DOI: 10.1111/j.1574-6968.2001.tb10604.x.
- Genetic Analysis of Assembly of theSalmonella enterica Serovar Typhimurium Type III Secretion-Associated Needle ComplexSukhan A, Kubori T, Wilson J, Galán J. Genetic Analysis of Assembly of theSalmonella enterica Serovar Typhimurium Type III Secretion-Associated Needle Complex Journal Of Bacteriology 2001, 183: 1159-1167. PMID: 11157927, PMCID: PMC94988, DOI: 10.1128/jb.183.4.1159-1167.2001.
- A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalizationZhou D, Chen L, Hernandez L, Shears S, Galán J. A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization Molecular Microbiology 2001, 39: 248-260. PMID: 11136447, DOI: 10.1046/j.1365-2958.2001.02230.x.
- Modulation of Host Signaling by a Bacterial Mimic Structure of the Salmonella Effector SptP Bound to Rac1Stebbins C, Galán J. Modulation of Host Signaling by a Bacterial Mimic Structure of the Salmonella Effector SptP Bound to Rac1 Molecular Cell 2000, 6: 1449-1460. PMID: 11163217, DOI: 10.1016/s1097-2765(00)00141-6.
- A Bacterial Toxin That Controls Cell Cycle Progression as a Deoxyribonuclease I-Like ProteinLara-Tejero M, Galán J. A Bacterial Toxin That Controls Cell Cycle Progression as a Deoxyribonuclease I-Like Protein Science 2000, 290: 354-357. PMID: 11030657, DOI: 10.1126/science.290.5490.354.
- Alternative Strategies for Becoming an Insider Lessons from the Bacterial WorldGalán J. Alternative Strategies for Becoming an Insider Lessons from the Bacterial World Cell 2000, 103: 363-366. PMID: 11081622, DOI: 10.1016/s0092-8674(00)00127-6.
- Biophysical characterization of SipA, an actin‐binding protein from Salmonella entericaMitra K, Zhou D, Galán J. Biophysical characterization of SipA, an actin‐binding protein from Salmonella enterica FEBS Letters 2000, 482: 81-84. PMID: 11018527, DOI: 10.1016/s0014-5793(00)02040-8.
- Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion systemKubori T, Sukhan A, Aizawa S, Galán J. Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 10225-10230. PMID: 10944190, PMCID: PMC27824, DOI: 10.1073/pnas.170128997.
- Striking a balance: Modulation of the actin cytoskeleton by SalmonellaGalán J, Zhou D. Striking a balance: Modulation of the actin cytoskeleton by Salmonella Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 8754-8761. PMID: 10922031, PMCID: PMC34008, DOI: 10.1073/pnas.97.16.8754.
- Developmental Control of Endocytosis in Dendritic Cells by Cdc42Garrett W, Chen L, Kroschewski R, Ebersold M, Turley S, Trombetta S, Galán J, Mellman I. Developmental Control of Endocytosis in Dendritic Cells by Cdc42 Cell 2000, 102: 325-334. PMID: 10975523, DOI: 10.1016/s0092-8674(00)00038-6.
- The Flagellar Sigma Factor FliA (ς28) Regulates the Expression of Salmonella Genes Associated with the Centisome 63 Type III Secretion SystemEichelberg K, Galán J. The Flagellar Sigma Factor FliA (ς28) Regulates the Expression of Salmonella Genes Associated with the Centisome 63 Type III Secretion System Infection And Immunity 2000, 68: 2735-2743. PMID: 10768967, PMCID: PMC97482, DOI: 10.1128/iai.68.5.2735-2743.2000.
- Complex Function for SicA, a Salmonella enterica Serovar Typhimurium Type III Secretion-Associated ChaperoneTucker S, Galán J. Complex Function for SicA, a Salmonella enterica Serovar Typhimurium Type III Secretion-Associated Chaperone Journal Of Bacteriology 2000, 182: 2262-2268. PMID: 10735870, PMCID: PMC111276, DOI: 10.1128/jb.182.8.2262-2268.2000.
- A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasionFu Y, Galán J. A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion Nature 1999, 401: 293-297. PMID: 10499590, DOI: 10.1038/45829.
- An invasion-associated Salmonella protein modulates the actin-bundling activity of plastinZhou D, Mooseker M, Galán J. An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 10176-10181. PMID: 10468582, PMCID: PMC17862, DOI: 10.1073/pnas.96.18.10176.
- Differential regulation of Salmonella typhimurium type III secreted proteins by pathogenicity island 1 (SPI-1)-encoded transcriptional activators InvF and hilA.Eichelberg K, Galán J. Differential regulation of Salmonella typhimurium type III secreted proteins by pathogenicity island 1 (SPI-1)-encoded transcriptional activators InvF and hilA. Infection And Immunity 1999, 67: 4099-105. PMID: 10417179, PMCID: PMC96710, DOI: 10.1128/iai.67.8.4099-4105.1999.
- Characterization of SprA, an AraC‐like transcriptional regulator encoded within the Salmonella typhimurium pathogenicity island 1Eichelberg K, Hardt W, Galán J. Characterization of SprA, an AraC‐like transcriptional regulator encoded within the Salmonella typhimurium pathogenicity island 1 Molecular Microbiology 1999, 33: 139-152. PMID: 10411731, DOI: 10.1046/j.1365-2958.1999.01458.x.
- Type III Secretion Machines: Bacterial Devices for Protein Delivery into Host CellsGalán J, Collmer A. Type III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells Science 1999, 284: 1322-1328. PMID: 10334981, DOI: 10.1126/science.284.5418.1322.
- Requirement of p21-activated Kinase (PAK) for Salmonella typhimurium–induced Nuclear ResponsesChen L, Bagrodia S, Cerione R, Galán J. Requirement of p21-activated Kinase (PAK) for Salmonella typhimurium–induced Nuclear Responses Journal Of Experimental Medicine 1999, 189: 1479-1488. PMID: 10224288, PMCID: PMC2193063, DOI: 10.1084/jem.189.9.1479.
- Salmonella typhimurium encodes a putative iron transport system within the centisome 63 pathogenicity island.Zhou D, Hardt W, Galán J. Salmonella typhimurium encodes a putative iron transport system within the centisome 63 pathogenicity island. Infection And Immunity 1999, 67: 1974-81. PMID: 10085045, PMCID: PMC96555, DOI: 10.1128/iai.67.4.1974-1981.1999.
- Role of the S. typhimurium Actin-Binding Protein SipA in Bacterial InternalizationZhou D, Mooseker M, Galán J. Role of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization Science 1999, 283: 2092-2095. PMID: 10092234, DOI: 10.1126/science.283.5410.2092.
- Interaction of Salmonella with host cells through the centisome 63 type III secretion systemGalán J. Interaction of Salmonella with host cells through the centisome 63 type III secretion system Current Opinion In Microbiology 1999, 2: 46-50. PMID: 10047557, DOI: 10.1016/s1369-5274(99)80008-3.
- Interaction of Salmonella spp. with host cellsMurli S, Galán J. Interaction of Salmonella spp. with host cells 1999, 6: 173-180. DOI: 10.1016/s1874-5172(99)80011-x.
- Salmonella typhimurium Encodes a Putative Iron Transport System within the Centisome 63 Pathogenicity IslandZhou D, Hardt W, Galán J. Salmonella typhimurium Encodes a Putative Iron Transport System within the Centisome 63 Pathogenicity Island Infection And Immunity 1999, 67: 1974-1981. DOI: 10.1128/.67.4.1974-1981.1999.
- Interactions of Salmonella with host cells: Encounters of the closest kindGalán J. Interactions of Salmonella with host cells: Encounters of the closest kind Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 14006-14008. PMID: 9826642, PMCID: PMC33922, DOI: 10.1073/pnas.95.24.14006.
- Delivery of Epitopes by the Salmonella Type III Secretion System for Vaccine DevelopmentRüssmann H, Shams H, Poblete F, Fu Y, Galán J, Donis R. Delivery of Epitopes by the Salmonella Type III Secretion System for Vaccine Development Science 1998, 281: 565-568. PMID: 9677200, DOI: 10.1126/science.281.5376.565.
- Identification of a specific chaperone for SptP, a substrate of the centisome 63 type III secretion system of Salmonella typhimurium.Fu Y, Galán J. Identification of a specific chaperone for SptP, a substrate of the centisome 63 type III secretion system of Salmonella typhimurium. Journal Of Bacteriology 1998, 180: 3393-9. PMID: 9642193, PMCID: PMC107295, DOI: 10.1128/jb.180.13.3393-3399.1998.
- S. typhimurium Encodes an Activator of Rho GTPases that Induces Membrane Ruffling and Nuclear Responses in Host CellsHardt W, Chen L, Schuebel K, Bustelo X, Galán J. S. typhimurium Encodes an Activator of Rho GTPases that Induces Membrane Ruffling and Nuclear Responses in Host Cells Cell 1998, 93: 815-826. PMID: 9630225, DOI: 10.1016/s0092-8674(00)81442-7.
- Supramolecular Structure of the Salmonella typhimurium Type III Protein Secretion SystemKubori T, Matsushima Y, Nakamura D, Uralil J, Lara-Tejero M, Sukhan A, Galán J, Aizawa S. Supramolecular Structure of the Salmonella typhimurium Type III Protein Secretion System Science 1998, 280: 602-605. PMID: 9554854, DOI: 10.1126/science.280.5363.602.
- A substrate of the centisome 63 type III protein secretion system of Salmonella typhimurium is encoded by a cryptic bacteriophageHardt W, Urlaub H, Galán J. A substrate of the centisome 63 type III protein secretion system of Salmonella typhimurium is encoded by a cryptic bacteriophage Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 2574-2579. PMID: 9482928, PMCID: PMC19418, DOI: 10.1073/pnas.95.5.2574.
- YopJ of Yersinia pseudotuberculosis is required for the inhibition of macrophage TNF‐α production and downregulation of the MAP kinases p38 and JNKPalmer L, Hobbie S, Galán J, Bliska J. YopJ of Yersinia pseudotuberculosis is required for the inhibition of macrophage TNF‐α production and downregulation of the MAP kinases p38 and JNK Molecular Microbiology 1998, 27: 953-965. PMID: 9535085, DOI: 10.1046/j.1365-2958.1998.00740.x.
- The Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeletonFu Y, Galán J. The Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeleton Molecular Microbiology 1998, 27: 359-368. PMID: 9484891, DOI: 10.1046/j.1365-2958.1998.00684.x.
- ‘Avirulence genes’ in animal pathogens?Galán J. ‘Avirulence genes’ in animal pathogens? Trends In Microbiology 1998, 6: 3-6. PMID: 9481815, DOI: 10.1016/s0966-842x(97)01183-9.
- A secreted Salmonella protein with homology to an avirulence determinant of plant pathogenic bacteriaHardt W, Galán J. A secreted Salmonella protein with homology to an avirulence determinant of plant pathogenic bacteria Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 9887-9892. PMID: 9275221, PMCID: PMC23287, DOI: 10.1073/pnas.94.18.9887.
- The invasion-associated type-III protein secretion system inSalmonella – a review1Presented at the Workshop on `Type-4 Pili – Biogenesis, Adhesins, Protein Export, and DNA Import', Schloss Ringberg, Germany, 26–29 November 1995.1Collazo C, Galán J. The invasion-associated type-III protein secretion system inSalmonella – a review1Presented at the Workshop on `Type-4 Pili – Biogenesis, Adhesins, Protein Export, and DNA Import', Schloss Ringberg, Germany, 26–29 November 1995.1 Gene 1997, 192: 51-59. PMID: 9224874, DOI: 10.1016/s0378-1119(96)00825-6.
- The invasion‐associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cellCollazo C, Galán J. The invasion‐associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell Molecular Microbiology 1997, 24: 747-756. PMID: 9194702, DOI: 10.1046/j.1365-2958.1997.3781740.x.
- Requirement of CDC42 for Salmonella-Induced Cytoskeletal and Nuclear ResponsesChen L, Hobbie S, Galán J. Requirement of CDC42 for Salmonella-Induced Cytoskeletal and Nuclear Responses Science 1996, 274: 2115-2118. PMID: 8953049, DOI: 10.1126/science.274.5295.2115.
- CROSS-TALK BETWEEN BACTERIAL PATHOGENS AND THEIR HOST CELLSGalán J, Bliska J. CROSS-TALK BETWEEN BACTERIAL PATHOGENS AND THEIR HOST CELLS Annual Review Of Cell And Developmental Biology 1996, 12: 221-255. PMID: 8970727, DOI: 10.1146/annurev.cellbio.12.1.221.
- Salmonella spp. are cytotoxic for cultured macrophagesChen L, Kaniga K, Galán J. Salmonella spp. are cytotoxic for cultured macrophages Molecular Microbiology 1996, 21: 1101-1115. PMID: 8885278, DOI: 10.1046/j.1365-2958.1996.471410.x.
- A secreted protein tyrosine phosphatase with modular effector domains in the bacterial pathogen Salmonella typhimurlumKaniga K, Uralil J, Bliska J, Galán J. A secreted protein tyrosine phosphatase with modular effector domains in the bacterial pathogen Salmonella typhimurlum Molecular Microbiology 1996, 21: 633-641. PMID: 8866485, DOI: 10.1111/j.1365-2958.1996.tb02571.x.
- Molecular genetic bases of Salmonella entry into host cellsGalán J. Molecular genetic bases of Salmonella entry into host cells Molecular Microbiology 1996, 20: 263-271. PMID: 8733226, DOI: 10.1111/j.1365-2958.1996.tb02615.x.
- Salmonella typhimurium InvA expression probed with a monoclonal antibody to the C-terminal peptide of InvACLARK C, MACDONALD L, GINOCCHIO C, GALAN J, JOHNSON R. Salmonella typhimurium InvA expression probed with a monoclonal antibody to the C-terminal peptide of InvA FEMS Microbiology Letters 1996, 136: 263-268. DOI: 10.1016/0378-1097(96)00008-0.
- Interactions of bacteria with non-phagocytic cellsGalan J. Interactions of bacteria with non-phagocytic cells Current Opinion In Immunology 1994, 6: 590-595. PMID: 7946047, DOI: 10.1016/0952-7915(94)90146-5.
- The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteinsKaniga K, Bossio J, Galán J. The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins Molecular Microbiology 1994, 13: 555-568. PMID: 7997169, DOI: 10.1111/j.1365-2958.1994.tb00450.x.
- Salmonella entry into mammalian cells: different yet converging signal transduction pathways?Galán J. Salmonella entry into mammalian cells: different yet converging signal transduction pathways? Trends In Cell Biology 1994, 4: 196-199. PMID: 14731677, DOI: 10.1016/0962-8924(94)90136-8.
- The molecular genetic bases of Salmonella entry into mammalian cellsGalán J, Ginocchio C. The molecular genetic bases of Salmonella entry into mammalian cells Biochemical Society Transactions 1994, 22: 301-306. PMID: 7958312, DOI: 10.1042/bst0220301.
- Contact with epithelial cells induces the formation of surface appendages on Salmonella typhimuriumGinocchio C, Olmsted S, Wells C, Galán J. Contact with epithelial cells induces the formation of surface appendages on Salmonella typhimurium Cell 1994, 76: 717-724. PMID: 8124710, DOI: 10.1016/0092-8674(94)90510-x.
- Signal transduction in the mammalian cell during bacterial attachment and entryBliska J, Galán J, Falkow S. Signal transduction in the mammalian cell during bacterial attachment and entry Cell 1993, 73: 903-920. PMID: 8500180, DOI: 10.1016/0092-8674(93)90270-z.
- Signal transduction and invasion of epithelial cells by S. typhimuriumPace J, Hayman M, Galán J. Signal transduction and invasion of epithelial cells by S. typhimurium Cell 1993, 72: 505-514. PMID: 8382566, DOI: 10.1016/0092-8674(93)90070-7.
- Cloning and molecular characterization of a gene involved in Salmonella adherence and invasion of cultured epithelial cellsAltmeyer R, McNern J, Bossio JC, Rosenshine I, Finlay B, Galán J. Cloning and molecular characterization of a gene involved in Salmonella adherence and invasion of cultured epithelial cells Molecular Microbiology 1993, 7: 89-98. PMID: 8382333, DOI: 10.1111/j.1365-2958.1993.tb01100.x.
- Colonization and Invasion of the Intestinal Tract by SalmonellaCurtiss R, MacLeod D, Lockman H, Galan J, Kelly S, Mahairas G. Colonization and Invasion of the Intestinal Tract by Salmonella 1993, 191-198. DOI: 10.1007/978-1-4615-2854-8_22.
- Involvement of the epidermal growth factor receptor in the invasion of cultured mammalian cells by Salmonella typhimuriumGalán J, Pace J, Hayman M. Involvement of the epidermal growth factor receptor in the invasion of cultured mammalian cells by Salmonella typhimurium Nature 1992, 357: 588-589. PMID: 1608468, DOI: 10.1038/357588a0.
- Colonization of The Murine Gastrointestinal Tract by Salmonella typhimuriumCurtiss R, Galán J. Colonization of The Murine Gastrointestinal Tract by Salmonella typhimurium 1991, 197-206. DOI: 10.1007/978-1-4684-5982-1_25.
- Cloning and characterization of the asd gene of Salmonella typhimurium: use in stable maintenance of recombinant plasmids in Salmonella vaccine strainsGalán J, Nakayama K, Curtiss R. Cloning and characterization of the asd gene of Salmonella typhimurium: use in stable maintenance of recombinant plasmids in Salmonella vaccine strains Gene 1990, 94: 29-35. PMID: 2227450, DOI: 10.1016/0378-1119(90)90464-3.
- Virulence and vaccine potential of phoP mutants of Salmonella typhimuriumGalán J, Curtiss R. Virulence and vaccine potential of phoP mutants of Salmonella typhimurium Microbial Pathogenesis 1989, 6: 433-443. PMID: 2671582, DOI: 10.1016/0882-4010(89)90085-5.