James E Rothman, PhD

• Erdem Karatekin
• Feng Li
• Frederic Pincet
• Jeff Coleman
• Karin Reinisch
• Shyam Krishnakumar

Biochemistry; Biophysics; Chemistry; Cell Biology; Golgi Apparatus; Membrane Fusion; Synaptic Transmission; Vesicular Transport Proteins

• A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusionKrishnakumar SS, Radoff DT, Kümmel D, Giraudo CG, Li F, Khandan L, Baguley SW, Coleman J, Reinisch KM, Pincet F, Rothman JE. A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusion Nature Structural & Molecular Biology 2011, 18: 934-940. PMID: 21785412, PMCID: PMC3668341, DOI: 10.1038/nsmb.2103.
• Complexin activates and clamps SNAREpins by a common mechanism involving an intermediate energetic stateLi F, Pincet F, Perez E, Giraudo CG, Tareste D, Rothman JE. Complexin activates and clamps SNAREpins by a common mechanism involving an intermediate energetic state Nature Structural & Molecular Biology 2011, 18: 941-946. PMID: 21785413, PMCID: PMC3736826, DOI: 10.1038/nsmb.2102.
• Complexin cross-links prefusion SNAREs into a zigzag arrayKümmel D, Krishnakumar SS, Radoff DT, Li F, Giraudo CG, Pincet F, Rothman JE, Reinisch KM. Complexin cross-links prefusion SNAREs into a zigzag array Nature Structural & Molecular Biology 2011, 18: 927-933. PMID: 21785414, PMCID: PMC3410656, DOI: 10.1038/nsmb.2101.
• Induction of cortical endoplasmic reticulum by dimerization of a coatomer-binding peptide anchored to endoplasmic reticulum membranesLavieu G, Orci L, Shi L, Geiling M, Ravazzola M, Wieland F, Cosson P, Rothman JE. Induction of cortical endoplasmic reticulum by dimerization of a coatomer-binding peptide anchored to endoplasmic reticulum membranes Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6876-6881. PMID: 20351264, PMCID: PMC2872465, DOI: 10.1073/pnas.1002536107.
• A fast, single-vesicle fusion assay mimics physiological SNARE requirementsKaratekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, Rothman JE. A fast, single-vesicle fusion assay mimics physiological SNARE requirements Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 3517-3521. PMID: 20133592, PMCID: PMC2840481, DOI: 10.1073/pnas.0914723107.