Frederic Pincet, PhD

Associate Research Scientist in Cell Biology

Research & Publications
Biography

Research Summary

I am a CNRS scientist from the Laboratoire de Physique Statistique of the Ecole Normale Supérieure in Paris, France, where I am interested in various interactions of membranes and/or single molecules. During my sabbatical stay in the laboratory of Prof. J. Rothman I am studying the thermo-mechanical impact of SNAREpins on membranes by combining theoretical predictions and micromanipulation experiments.

Coauthors

Selected Publications

Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayerPanda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.
2-d liquid-liquid phase separation of GM130 Golgin protein bound to supported bilayerGoder J, Su M, Radhakrishnan A, Lopez-Montero I, Pincet F, Rothman J. 2-d liquid-liquid phase separation of GM130 Golgin protein bound to supported bilayer. Biophysical Journal 2023, 122: 457a. DOI: 10.1016/j.bpj.2022.11.2460.
The Get1/2 insertase forms a channel to mediate the insertion of tail-anchored proteins into the ERHeo P, Culver J, Miao J, Pincet F, Mariappan M. The Get1/2 insertase forms a channel to mediate the insertion of tail-anchored proteins into the ER. Cell Reports 2022, 42: 111921. PMID: 36640319, PMCID: PMC9932932, DOI: 10.1016/j.celrep.2022.111921.
Actuating tension-loaded DNA clamps drives membrane tubulationLiu L, Xiong Q, Xie C, Pincet F, Lin C. Actuating tension-loaded DNA clamps drives membrane tubulation. Science Advances 2022, 8: eadd1830. PMID: 36223466, PMCID: PMC9555772, DOI: 10.1126/sciadv.add1830.
The beginning and the end of SNARE‐induced membrane fusionMion D, Bunel L, Heo P, Pincet F. The beginning and the end of SNARE‐induced membrane fusion. FEBS Open Bio 2022, 12: 1958-1979. PMID: 35622519, PMCID: PMC9623537, DOI: 10.1002/2211-5463.13447.
CX3CL1 homo-oligomerization drives cell-to-cell adherenceOstuni M, Hermand P, Saindoy E, Guillou N, Guellec J, Coens A, Hattab C, Desuzinges-Mandon E, Jawhari A, Iatmanen-Harbi S, Lequin O, Fuchs P, Lacapere J, Combadière C, Pincet F, Deterre P. CX3CL1 homo-oligomerization drives cell-to-cell adherence. Scientific Reports 2020, 10: 9069. PMID: 32494000, PMCID: PMC7271195, DOI: 10.1038/s41598-020-65988-w.
TANGO1 membrane helices create a lipid diffusion barrier at curved membranesRaote I, Ernst AM, Campelo F, Rothman JE, Pincet F, Malhotra V. TANGO1 membrane helices create a lipid diffusion barrier at curved membranes. ELife 2020, 9: e57822. PMID: 32452385, PMCID: PMC7266638, DOI: 10.7554/elife.57822.
In vitro physiological membrane‐on‐a‐chip and its application in cell and neuronal biologyHeo P, Rothman J, Pincet F. In vitro physiological membrane‐on‐a‐chip and its application in cell and neuronal biology. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.08637.
Freezing and piercing of in vitro asymmetric plasma membrane by α-synucleinHeo P, Pincet F. Freezing and piercing of in vitro asymmetric plasma membrane by α-synuclein. Communications Biology 2020, 3: 148. PMID: 32235856, PMCID: PMC7109109, DOI: 10.1038/s42003-020-0883-7.
Munc13 Recruits SNAP25 to Facilitate SNARE Complex AssemblySundaram R, Li F, Coleman J, Pincet F, Rothman J, Krishnakumar S. Munc13 Recruits SNAP25 to Facilitate SNARE Complex Assembly. Biophysical Journal 2020, 118: 400a-401a. DOI: 10.1016/j.bpj.2019.11.2274.
Dissecting the Synergistic Roles of Synaptotagmin and Complexin in Ca2+-Regulated ExocytosisRamakrishnan S, Bera M, Coleman J, Pincet F, Rothman J, Krishnakumar S. Dissecting the Synergistic Roles of Synaptotagmin and Complexin in Ca2+-Regulated Exocytosis. Biophysical Journal 2020, 118: 488a. DOI: 10.1016/j.bpj.2019.11.2702.
Munc13 Clusters Capture Vesicles to Lipid Bilayer MembraneLi F, Sundaram R, Coleman J, Krishnakumar S, Pincet F, Rothman J. Munc13 Clusters Capture Vesicles to Lipid Bilayer Membrane. Biophysical Journal 2020, 118: 344a. DOI: 10.1016/j.bpj.2019.11.1990.
Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D‐Printed Microfluidic SetupHeo P, Ramakrishnan S, Coleman J, Rothman JE, Fleury J, Pincet F. Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D‐Printed Microfluidic Setup. Small 2019, 15: e1900725. PMID: 30977975, DOI: 10.1002/smll.201900725.
SNARE machinery is optimized for ultrafast fusionManca F, Pincet F, Truskinovsky L, Rothman JE, Foret L, Caruel M. SNARE machinery is optimized for ultrafast fusion. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 2435-2442. PMID: 30700546, PMCID: PMC6377469, DOI: 10.1073/pnas.1820394116.
High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated AnalysisRamakrishnan S, Gohlke A, Li F, Coleman J, Xu W, Rothman JE, Pincet F. High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis. Langmuir 2018, 34: 5849-5859. PMID: 29694054, DOI: 10.1021/acs.langmuir.8b00116.
Vesicle Tubulation with Self‐Assembling DNA NanospringsGrome M, Zhang Z, Pincet F, Lin C. Vesicle Tubulation with Self‐Assembling DNA Nanosprings. Angewandte Chemie 2018, 130: 5428-5432. DOI: 10.1002/ange.201800141.
Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+Gruget C, Coleman J, Bello O, Krishnakumar SS, Perez E, Rothman JE, Pincet F, Donaldson SH. Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+. FEBS Letters 2018, 592: 1497-1506. PMID: 29578584, DOI: 10.1002/1873-3468.13040.
Direct Measurement of Site-Specific Binding Energetics of Synaptotagmin-1 with Anionic Lipid MembranesGruget C, Coleman J, Bello O, Krishnakumar S, Pincet F, Rothman J, Donaldson S. Direct Measurement of Site-Specific Binding Energetics of Synaptotagmin-1 with Anionic Lipid Membranes. Biophysical Journal 2018, 114: 607a. DOI: 10.1016/j.bpj.2017.11.3318.
Correction: Re-visiting the trans insertion model for complexin clampingKrishnakumar SS, Li F, Coleman J, Schauder CM, Kümmel D, Pincet F, Rothman JE, Reinisch KM. Correction: Re-visiting the trans insertion model for complexin clamping. ELife 2017, 6: e31512. PMID: 28880147, PMCID: PMC5589411, DOI: 10.7554/elife.31512.
Endothelial basement membrane laminin 511 is essential for shear stress responseDi Russo J, Luik A, Yousif L, Budny S, Oberleithner H, Hofschröer V, Klingauf J, van Bavel E, Bakker E, Hellstrand P, Bhattachariya A, Albinsson S, Pincet F, Hallmann R, Sorokin L. Endothelial basement membrane laminin 511 is essential for shear stress response. The EMBO Journal 2017, 36: 1464-1464. PMID: 28507085, PMCID: PMC5430226, DOI: 10.15252/embj.201797000.
Axon tension regulates fasciculation/defasciculation through the control of axon shaft zipperingŠmít D, Fouquet C, Pincet F, Zapotocky M, Trembleau A. Axon tension regulates fasciculation/defasciculation through the control of axon shaft zippering. ELife 2017, 6: e19907. PMID: 28422009, PMCID: PMC5478281, DOI: 10.7554/elife.19907.
Actual fusion efficiency in the lipid mixing assay - Comparison between nanodiscs and liposomesFrançois-Martin C, Pincet F. Actual fusion efficiency in the lipid mixing assay - Comparison between nanodiscs and liposomes. Scientific Reports 2017, 7: 43860. PMID: 28266607, PMCID: PMC5339690, DOI: 10.1038/srep43860.
BFPTool: a software tool for analysis of Biomembrane Force Probe experimentsŠmít D, Fouquet C, Doulazmi M, Pincet F, Trembleau A, Zapotocky M. BFPTool: a software tool for analysis of Biomembrane Force Probe experiments. BMC Biophysics 2017, 10: 2. PMID: 28289540, PMCID: PMC5304404, DOI: 10.1186/s13628-016-0033-2.
The Bilat: A Free Standing Lipid Bilayer Microarray Platform for Membrane FusionRamakrishnan S, Gohlke A, Heo P, Rothman J, Pincet F. The Bilat: A Free Standing Lipid Bilayer Microarray Platform for Membrane Fusion. Biophysical Journal 2017, 112: 77a. DOI: 10.1016/j.bpj.2016.11.461.
Synaptotagmin Interactions with Membranes: Measuring the Force of Calcium Triggering of NeurotransmissionGruget C, Coleman J, Krishankumar S, Rothman J, Pincet F, Donaldson S. Synaptotagmin Interactions with Membranes: Measuring the Force of Calcium Triggering of Neurotransmission. Biophysical Journal 2017, 112: 34a-35a. DOI: 10.1016/j.bpj.2016.11.220.
Endothelial basement membrane laminin 511 is essential for shear stress responseDi Russo J, Luik A, Yousif L, Budny S, Oberleithner H, Hofschröer V, Klingauf J, van Bavel E, Bakker E, Hellstrand P, Bhattachariya A, Albinsson S, Pincet F, Hallmann R, Sorokin L. Endothelial basement membrane laminin 511 is essential for shear stress response. The EMBO Journal 2016, 36: 183-201. PMID: 27940654, PMCID: PMC5239996, DOI: 10.15252/embj.201694756.
FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane EnvironmentsPincet F, Adrien V, Yang R, Delacotte J, Rothman JE, Urbach W, Tareste D. FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane Environments. PLOS ONE 2016, 11: e0158457. PMID: 27387979, PMCID: PMC4936743, DOI: 10.1371/journal.pone.0158457.
On-Chip Quantitative Measurement of Mechanical Stresses During Cell Migration with Emulsion DropletsMolino D, Quignard S, Gruget C, Pincet F, Chen Y, Piel M, Fattaccioli J. On-Chip Quantitative Measurement of Mechanical Stresses During Cell Migration with Emulsion Droplets. Scientific Reports 2016, 6: 29113. PMID: 27373558, PMCID: PMC4931467, DOI: 10.1038/srep29113.
A Programmable DNA Origami Platform to Organize SNAREs for Membrane FusionXu W, Nathwani B, Lin C, Wang J, Karatekin E, Pincet F, Shih W, Rothman JE. A Programmable DNA Origami Platform to Organize SNAREs for Membrane Fusion. Journal Of The American Chemical Society 2016, 138: 4439-4447. PMID: 26938705, PMCID: PMC4950518, DOI: 10.1021/jacs.5b13107.
Experimental Measurement of the Activation Energy of Phospholipid Membrane FusionFrançois-Martin C, Rothman J, Pincet F. Experimental Measurement of the Activation Energy of Phospholipid Membrane Fusion. Biophysical Journal 2016, 110: 520a. DOI: 10.1016/j.bpj.2015.11.2778.
Axon zippering in neuronal cell culture and its biophysical modelingSmit D, Fouquet C, Pincet F, Trembleau A, Zapotocky M. Axon zippering in neuronal cell culture and its biophysical modeling. BMC Neuroscience 2015, 16: p298. PMCID: PMC4699001, DOI: 10.1186/1471-2202-16-s1-p298.
Accelerating SNARE‐Mediated Membrane Fusion by DNA–Lipid TethersXu W, Wang J, Rothman J, Pincet F. Accelerating SNARE‐Mediated Membrane Fusion by DNA–Lipid Tethers. Angewandte Chemie 2015, 127: 14596-14600. DOI: 10.1002/ange.201506844.
Correction to Formation of Giant Unilamellar Proteo-Liposomes by Osmotic ShockMotta I, Gohlke A, Adrien V, Li F, Gardavot H, Rothman JE, Pincet F. Correction to Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock. Langmuir 2015, 31: 9521-9521. PMID: 26293386, DOI: 10.1021/acs.langmuir.5b02955.
Formation of Giant Unilamellar Proteo-Liposomes by Osmotic ShockMotta I, Gohlke A, Adrien V, Li F, Gardavot H, Rothman JE, Pincet F. Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock. Langmuir 2015, 31: 7091-7099. PMID: 26038815, PMCID: PMC4950989, DOI: 10.1021/acs.langmuir.5b01173.
Assessing the Translocation of CPPs using Force Measurement, Single Molecules and EmulsionsSoule P, Thiam A, Pincet F, Joliot A, Sagan S, Cribier S, Rodriguez N. Assessing the Translocation of CPPs using Force Measurement, Single Molecules and Emulsions. Biophysical Journal 2015, 108: 497a. DOI: 10.1016/j.bpj.2014.11.2720.
CX3CL1, a chemokine finely tuned to adhesion: critical roles of the stalk glycosylation and the membrane domainOstuni M, Guellec J, Hermand P, Durand P, Combadière C, Pincet F, Deterre P. CX3CL1, a chemokine finely tuned to adhesion: critical roles of the stalk glycosylation and the membrane domain. Biology Open 2014, 3: 1173-1182. PMID: 25395671, PMCID: PMC4265755, DOI: 10.1242/bio.20149845.
Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilizationChalbi M, Barraud-Lange V, Ravaux B, Howan K, Rodriguez N, Soule P, Ndzoudi A, Boucheix C, Rubinstein E, Wolf J, Ziyyat A, Perez E, Pincet F, Gourier C. Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilization. Development 2014, 141: 3732-3739. PMID: 25209248, DOI: 10.1242/dev.111534.
Calcium sensitive ring-like oligomers formed by synaptotagminWang J, Bello O, Auclair SM, Wang J, Coleman J, Pincet F, Krishnakumar SS, Sindelar CV, Rothman JE. Calcium sensitive ring-like oligomers formed by synaptotagmin. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 13966-13971. PMID: 25201968, PMCID: PMC4183308, DOI: 10.1073/pnas.1415849111.
A Half-Zippered SNARE Complex Represents a Functional Intermediate in Membrane FusionLi F, Kümmel D, Coleman J, Reinisch KM, Rothman JE, Pincet F. A Half-Zippered SNARE Complex Represents a Functional Intermediate in Membrane Fusion. Journal Of The American Chemical Society 2014, 136: 3456-3464. PMID: 24533674, PMCID: PMC3985920, DOI: 10.1021/ja410690m.
Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targetingWilfling F, Thiam AR, Olarte MJ, Wang J, Beck R, Gould TJ, Allgeyer ES, Pincet F, Bewersdorf J, Farese RV, Walther TC. Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting. ELife 2014, 3: e01607. PMID: 24497546, PMCID: PMC3913038, DOI: 10.7554/elife.01607.
Comparative Study of the Snares Zippering with Single Molecule ResolutionZorman S, Pincet F, Rothman J, Zhang Y. Comparative Study of the Snares Zippering with Single Molecule Resolution. Biophysical Journal 2014, 106: 506a. DOI: 10.1016/j.bpj.2013.11.2831.
Homotypic and Heterotypic Adhesion Induced by Odorant Receptors and the β2-Adrenergic ReceptorRichard M, Jamet S, Fouquet C, Dubacq C, Boggetto N, Pincet F, Gourier C, Trembleau A. Homotypic and Heterotypic Adhesion Induced by Odorant Receptors and the β2-Adrenergic Receptor. PLOS ONE 2013, 8: e80100. PMID: 24312457, PMCID: PMC3846556, DOI: 10.1371/journal.pone.0080100.
COPI buds 60-nm lipid droplets from reconstituted water–phospholipid–triacylglyceride interfaces, suggesting a tension clamp functionThiam AR, Antonny B, Wang J, Delacotte J, Wilfling F, Walther TC, Beck R, Rothman JE, Pincet F. COPI buds 60-nm lipid droplets from reconstituted water–phospholipid–triacylglyceride interfaces, suggesting a tension clamp function. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 13244-13249. PMID: 23901109, PMCID: PMC3746913, DOI: 10.1073/pnas.1307685110.
Preparation and characterization of SNARE-containing nanodiscs and direct study of cargo release through fusion poresShi L, Howan K, Shen QT, Wang YJ, Rothman JE, Pincet F. Preparation and characterization of SNARE-containing nanodiscs and direct study of cargo release through fusion pores. Nature Protocols 2013, 8: 935-948. PMID: 23598444, DOI: 10.1038/nprot.2013.048.
BioadhesionPerez E, Pincet F. Bioadhesion. 2013, 241-277. DOI: 10.1142/9789814440592_0006.
SNARE Proteins: One to Fuse and Three to Keep the Nascent Fusion Pore OpenShi L, Shen QT, Kiel A, Wang J, Wang HW, Melia TJ, Rothman JE, Pincet F. SNARE Proteins: One to Fuse and Three to Keep the Nascent Fusion Pore Open. Science 2012, 335: 1355-1359. PMID: 22422984, PMCID: PMC3736847, DOI: 10.1126/science.1214984.
Complexin cross-links prefusion SNAREs into a zigzag arrayKümmel D, Krishnakumar SS, Radoff DT, Li F, Giraudo CG, Pincet F, Rothman JE, Reinisch KM. Complexin cross-links prefusion SNAREs into a zigzag array. Nature Structural & Molecular Biology 2011, 18: 927-933. PMID: 21785414, PMCID: PMC3410656, DOI: 10.1038/nsmb.2101.
A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusionKrishnakumar SS, Radoff DT, Kümmel D, Giraudo CG, Li F, Khandan L, Baguley SW, Coleman J, Reinisch KM, Pincet F, Rothman JE. A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusion. Nature Structural & Molecular Biology 2011, 18: 934-940. PMID: 21785412, PMCID: PMC3668341, DOI: 10.1038/nsmb.2103.
Complexin activates and clamps SNAREpins by a common mechanism involving an intermediate energetic stateLi F, Pincet F, Perez E, Giraudo CG, Tareste D, Rothman JE. Complexin activates and clamps SNAREpins by a common mechanism involving an intermediate energetic state. Nature Structural & Molecular Biology 2011, 18: 941-946. PMID: 21785413, PMCID: PMC3736826, DOI: 10.1038/nsmb.2102.
CD9 tetraspanin generates fusion competent sites on the egg membrane for mammalian fertilizationJégou A, Ziyyat A, Barraud-Lange V, Perez E, Wolf J, Pincet F, Gourier C. CD9 tetraspanin generates fusion competent sites on the egg membrane for mammalian fertilization. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 10946-10951. PMID: 21690351, PMCID: PMC3131345, DOI: 10.1073/pnas.1017400108.
Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-MacromoleculesRayan G, Guet J, Taulier N, Pincet F, Urbach W. Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules. Sensors 2010, 10: 5927-5948. PMID: 22219695, PMCID: PMC3247740, DOI: 10.3390/s100605927.
Integrins stimulate E-cadherin-mediated intercellular adhesion by regulating Src-kinase activation and actomyosin contractilityMartinez-Rico C, Pincet F, Thiery J, Dufour S. Integrins stimulate E-cadherin-mediated intercellular adhesion by regulating Src-kinase activation and actomyosin contractility. Development 2010, 137: e1-e1. DOI: 10.1242/dev.050492.
Integrins stimulate E-cadherin-mediated intercellular adhesion by regulating Src-kinase activation and actomyosin contractilityMartinez-Rico C, Pincet F, Thiery JP, Dufour S. Integrins stimulate E-cadherin-mediated intercellular adhesion by regulating Src-kinase activation and actomyosin contractility. Journal Of Cell Science 2010, 123: 712-722. PMID: 20144995, DOI: 10.1242/jcs.047878.
The adhesion mediated by the P‐selectin P–selectin glycoprotein ligand‐1 (PSGL‐1) couple is stronger for shorter PSGL‐1 variantsBarbaux S, Poirier O, Pincet F, Hermand P, Tiret L, Deterre P. The adhesion mediated by the P‐selectin P–selectin glycoprotein ligand‐1 (PSGL‐1) couple is stronger for shorter PSGL‐1 variants. Journal Of Leukocyte Biology 2010, 87: 727-734. PMID: 20051472, DOI: 10.1189/jlb.0609408.
Quantification of phase transitions of lipid mixtures from bilayer to non-bilayer structures: Model, experimental validation and implication on membrane fusionXu W, Pincet F. Quantification of phase transitions of lipid mixtures from bilayer to non-bilayer structures: Model, experimental validation and implication on membrane fusion. Chemistry And Physics Of Lipids 2009, 163: 280-285. PMID: 20018184, DOI: 10.1016/j.chemphyslip.2009.12.002.
Two-dimensional crystallization of hard sphere particles at a liquid–liquid interfaceMarnette O, Perez E, Pincet F, Bryant G. Two-dimensional crystallization of hard sphere particles at a liquid–liquid interface. Colloids And Surfaces A Physicochemical And Engineering Aspects 2009, 346: 208-212. DOI: 10.1016/j.colsurfa.2009.06.018.
Nanoforce and ImagingLe Grimellec C, Milhiet P, Perez E, Pincet F, Aimé J, Emiliani V, Thoumine O, Lionnet T, Croquette V, Allemand J, Bensimon D. Nanoforce and Imaging. 2009, 375-475. DOI: 10.1007/978-3-540-88633-4_8.
Force spectroscopy of a single artificial biomolecule bond: The Kramers’ high-barrier limit holds close to the critical forceHusson J, Dogterom M, Pincet F. Force spectroscopy of a single artificial biomolecule bond: The Kramers’ high-barrier limit holds close to the critical force. The Journal Of Chemical Physics 2009, 130: 051103. PMID: 19206951, DOI: 10.1063/1.3077010.
AdhesionPincet F. Adhesion. 2008, 22-31. DOI: 10.1201/noe0849396397.ch2.
Single Bonds and Adhesion in Biological MatterPerez E, Pincet F. Single Bonds and Adhesion in Biological Matter. 2008, 631-654. DOI: 10.1201/9781420007206.ch15.
A Nanospring Named Erythrocyte. The Biomembrane Force ProbeGourier C, Jegou A, Husson J, Pincet F. A Nanospring Named Erythrocyte. The Biomembrane Force Probe. Cellular And Molecular Bioengineering 2008, 1: 263. DOI: 10.1007/s12195-008-0030-x.
The Surface Force Apparatus to Reveal the Energetics of Biomolecules Assembly. Application to DNA Bases Pairing and SNARE Fusion Proteins FoldingPerez E, Li F, Tareste D, Pincet F. The Surface Force Apparatus to Reveal the Energetics of Biomolecules Assembly. Application to DNA Bases Pairing and SNARE Fusion Proteins Folding. Cellular And Molecular Bioengineering 2008, 1: 240. DOI: 10.1007/s12195-008-0025-7.
Functional Adhesiveness of the CX3CL1 Chemokine Requires Its Aggregation ROLE OF THE TRANSMEMBRANE DOMAIN*Hermand P, Pincet F, Carvalho S, Ansanay H, Trinquet E, Daoudi M, Combadière C, Deterre P. Functional Adhesiveness of the CX3CL1 Chemokine Requires Its Aggregation ROLE OF THE TRANSMEMBRANE DOMAIN*. Journal Of Biological Chemistry 2008, 283: 30225-30234. PMID: 18725411, PMCID: PMC2662081, DOI: 10.1074/jbc.m802638200.
Analyzing single-bond experiments: Influence of the shape of the energy landscape and universal law between the width, depth, and force spectrum of the bondHusson J, Pincet F. Analyzing single-bond experiments: Influence of the shape of the energy landscape and universal law between the width, depth, and force spectrum of the bond. Physical Review E 2008, 77: 026108. PMID: 18352091, DOI: 10.1103/physreve.77.026108.
Mapping Mouse Gamete Interaction Forces Reveal Several Oocyte Membrane Regions with Different Mechanical and Adhesive Properties †Jégou A, Pincet F, Perez E, Wolf JP, Ziyyat A, Gourier C. Mapping Mouse Gamete Interaction Forces Reveal Several Oocyte Membrane Regions with Different Mechanical and Adhesive Properties †. Langmuir 2007, 24: 1451-1458. PMID: 18027975, DOI: 10.1021/la702258x.
Confinement Free Energy of Surfaces Bearing End-Grafted Polymers in the Mushroom Regime and Local Measurement of the Polymer DensityLi F, Pincet F. Confinement Free Energy of Surfaces Bearing End-Grafted Polymers in the Mushroom Regime and Local Measurement of the Polymer Density. Langmuir 2007, 23: 12541-12548. PMID: 17988162, DOI: 10.1021/la7021374.
Energetics and dynamics of SNAREpin folding across lipid bilayersLi F, Pincet F, Perez E, Eng WS, Melia TJ, Rothman JE, Tareste D. Energetics and dynamics of SNAREpin folding across lipid bilayers. Nature Structural & Molecular Biology 2007, 14: 890-896. PMID: 17906638, DOI: 10.1038/nsmb1310.
Hydrophobic Forces and Hydrogen Bonds in the Adhesion between Retinoid-Coated SurfacesTareste D, Pincet F, Lebeau L, Perez É. Hydrophobic Forces and Hydrogen Bonds in the Adhesion between Retinoid-Coated Surfaces. Langmuir 2007, 23: 3225-3229. PMID: 17266339, DOI: 10.1021/la0629779.
Transition from long- to short-lived transient pores in giant vesicles in an aqueous mediumRodriguez N, Cribier S, Pincet F. Transition from long- to short-lived transient pores in giant vesicles in an aqueous medium. Physical Review E 2006, 74: 061902. PMID: 17280091, DOI: 10.1103/physreve.74.061902.
Spontaneous and Reversible Switch from Amphiphilic to Oil-Like StructuresPincet F, Tareste D, Amar M, Perez É. Spontaneous and Reversible Switch from Amphiphilic to Oil-Like Structures. Physical Review Letters 2005, 95: 218101. PMID: 16384186, DOI: 10.1103/physrevlett.95.218101.
Prototypical Type I E-cadherin and Type II Cadherin-7 Mediate Very Distinct Adhesiveness through Their Extracellular Domains*Chu YS, Eder O, Thomas WA, Simcha I, Pincet F, Ben-Ze'ev A, Perez E, Thiery JP, Dufour S. Prototypical Type I E-cadherin and Type II Cadherin-7 Mediate Very Distinct Adhesiveness through Their Extracellular Domains*. Journal Of Biological Chemistry 2005, 281: 2901-2910. PMID: 16253998, DOI: 10.1074/jbc.m506185200.
The Solution to the Streptavidin-Biotin Paradox: The Influence of History on the Strength of Single Molecular BondsPincet F, Husson J. The Solution to the Streptavidin-Biotin Paradox: The Influence of History on the Strength of Single Molecular Bonds. Biophysical Journal 2005, 89: 4374-4381. PMID: 16169976, PMCID: PMC1367001, DOI: 10.1529/biophysj.105.067769.
Indirect evidence of submicroscopic pores in giant unilamelar vesiclesRodriguez N, Heuvingh J, Pincet F, Cribier S. Indirect evidence of submicroscopic pores in giant unilamelar vesicles. Biochimica Et Biophysica Acta 2005, 1724: 281-287. PMID: 15978732, DOI: 10.1016/j.bbagen.2005.04.028.
Giant vesicles formed by gentle hydration and electroformation: A comparison by fluorescence microscopyRodriguez N, Pincet F, Cribier S. Giant vesicles formed by gentle hydration and electroformation: A comparison by fluorescence microscopy. Colloids And Surfaces B Biointerfaces 2005, 42: 125-130. PMID: 15833663, DOI: 10.1016/j.colsurfb.2005.01.010.
Influence of pH on Stability and Dynamic Properties of Asphaltenes and Other Amphiphilic Molecules at the Oil−Water Interface †Poteau S, Argillier J, Langevin D, Pincet F, Perez E. Influence of pH on Stability and Dynamic Properties of Asphaltenes and Other Amphiphilic Molecules at the Oil−Water Interface †. Energy & Fuels 2005, 19: 1337-1341. DOI: 10.1021/ef0497560.
The Natural LewisX‐Bearing Lipids Promote Membrane Adhesion: Influence of Ceramide on Carbohydrate–Carbohydrate RecognitionGourier C, Pincet F, Perez E, Zhang Y, Zhu Z, Mallet J, Sinaÿ P. The Natural LewisX‐Bearing Lipids Promote Membrane Adhesion: Influence of Ceramide on Carbohydrate–Carbohydrate Recognition. Angewandte Chemie 2005, 117: 1711-1715. DOI: 10.1002/ange.200461224.
The Natural LewisX‐Bearing Lipids Promote Membrane Adhesion: Influence of Ceramide on Carbohydrate–Carbohydrate RecognitionGourier C, Pincet F, Perez E, Zhang Y, Zhu Z, Mallet J, Sinaÿ P. The Natural LewisX‐Bearing Lipids Promote Membrane Adhesion: Influence of Ceramide on Carbohydrate–Carbohydrate Recognition. Angewandte Chemie International Edition 2005, 44: 1683-1687. PMID: 15693050, DOI: 10.1002/anie.200461224.
The Binding Energy of Two Nitrilotriacetate Groups Sharing a Nickel IonTareste D, Pincet F, Brellier M, Mioskowski C, Perez É. The Binding Energy of Two Nitrilotriacetate Groups Sharing a Nickel Ion. Journal Of The American Chemical Society 2005, 127: 3879-3884. PMID: 15771524, DOI: 10.1021/ja043525q.
Johnson-Kendall-Roberts Theory Applied to Living CellsChu YS, Dufour S, Thiery JP, Perez E, Pincet F. Johnson-Kendall-Roberts Theory Applied to Living Cells. Physical Review Letters 2005, 94: 028102. PMID: 15698233, DOI: 10.1103/physrevlett.94.028102.
Force measurements in E-cadherin–mediated cell doublets reveal rapid adhesion strengthened by actin cytoskeleton remodeling through Rac and Cdc42Chu Y, Thomas W, Eder O, Pincet F, Perez E, Thiery J, Dufour S. Force measurements in E-cadherin–mediated cell doublets reveal rapid adhesion strengthened by actin cytoskeleton remodeling through Rac and Cdc42. Journal Of Cell Biology 2004, 167: 1183-1194. PMID: 15596540, PMCID: PMC2172605, DOI: 10.1083/jcb.200403043.
Separation Force Measurements Reveal Different Types of Modulation of E-cadherin-based Adhesion by Nectin-1 and -3*Martinez-Rico C, Pincet F, Perez E, Thiery J, Shimizu K, Takai Y, Dufour S. Separation Force Measurements Reveal Different Types of Modulation of E-cadherin-based Adhesion by Nectin-1 and -3*. Journal Of Biological Chemistry 2004, 280: 4753-4760. PMID: 15550395, DOI: 10.1074/jbc.m412544200.
Can Small Complex Chains Be Treated as Polymers?Gourier C, Pincet F, Le Bouar T, Zhang Y, Esnault J, Mallet J, Sinay P, Perez E. Can Small Complex Chains Be Treated as Polymers? Macromolecules 2004, 37: 8778-8784. DOI: 10.1021/ma035905m.
Specific and non specific interactions involving LeX determinant quantified by lipid vesicle micromanipulationGourier C, Pincet F, Perez E, Zhang Y, Mallet J, Sinaÿ P. Specific and non specific interactions involving LeX determinant quantified by lipid vesicle micromanipulation. Glycoconjugate Journal 2004, 21: 165-174. PMID: 15483381, DOI: 10.1023/b:glyc.0000044847.15797.2e.
Hemifusion and fusion of giant vesicles induced by reduction of inter-membrane distanceHeuvingh J, Pincet F, Cribier S. Hemifusion and fusion of giant vesicles induced by reduction of inter-membrane distance. The European Physical Journal E 2004, 14: 269-276. PMID: 15338438, DOI: 10.1140/epje/i2003-10151-2.
Specific Recognition of Macroscopic Objects by the Cell Surface: Evidence for a Receptor Density Threshold Revealed by Micrometric Particle Binding CharacteristicsSarda S, Pointu D, Pincet F, Henry N. Specific Recognition of Macroscopic Objects by the Cell Surface: Evidence for a Receptor Density Threshold Revealed by Micrometric Particle Binding Characteristics. Biophysical Journal 2004, 86: 3291-3303. PMID: 15111442, PMCID: PMC1304194, DOI: 10.1016/s0006-3495(04)74377-5.
Enhanced Adhesive Capacities of the Naturally Occurring Ile249–Met280 Variant of the Chemokine Receptor CX3CR1*Daoudi M, Lavergne E, Garin A, Tarantino N, Debré P, Pincet F, Combadière C, Deterre P. Enhanced Adhesive Capacities of the Naturally Occurring Ile249–Met280 Variant of the Chemokine Receptor CX3CR1*. Journal Of Biological Chemistry 2004, 279: 19649-19657. PMID: 14990582, DOI: 10.1074/jbc.m313457200.
How hard is a colloidal “hard-sphere” interaction?Bryant G, Williams S, Qian L, Snook I, Perez E, Pincet F. How hard is a colloidal “hard-sphere” interaction? Physical Review E 2002, 66: 060501. PMID: 12513261, DOI: 10.1103/physreve.66.060501.
Energy of Hydrogen Bonds Probed by the Adhesion of Functionalized Lipid LayersTareste D, Pincet F, Perez E, Rickling S, Mioskowski C, Lebeau L. Energy of Hydrogen Bonds Probed by the Adhesion of Functionalized Lipid Layers. Biophysical Journal 2002, 83: 3675-3681. PMID: 12496134, PMCID: PMC1302442, DOI: 10.1016/s0006-3495(02)75367-8.
ChemInform Abstract: New Highly Hydrophobic Lewis X Glycolipids: Synthesis and Monolayer Behavior.Esnault J, Mallet J, Zhang Y, Sinay P, Le Bouar T, Pincet F, Perez E. ChemInform Abstract: New Highly Hydrophobic Lewis X Glycolipids: Synthesis and Monolayer Behavior. ChemInform 2002, 33: no-no. DOI: 10.1002/chin.200205216.
From Macroscopic Adhesion Energy to Molecular Bonds: A Test of the TheoryPincet F, Perez E, Loudet J, Lebeau L. From Macroscopic Adhesion Energy to Molecular Bonds: A Test of the Theory. Physical Review Letters 2001, 87: 178101. PMID: 11690314, DOI: 10.1103/physrevlett.87.178101.
Short-range specific forces are able to induce hemifusionPincet F, Lebeau L, Cribier S. Short-range specific forces are able to induce hemifusion. European Biophysics Journal 2001, 30: 91-97. PMID: 11409468, DOI: 10.1007/s002490100131.
Ultraweak Sugar-Sugar Interactions for Transient Cell AdhesionPincet F, Le Bouar T, Zhang Y, Esnault J, Mallet J, Perez E, Sinaÿ P. Ultraweak Sugar-Sugar Interactions for Transient Cell Adhesion. Biophysical Journal 2001, 80: 1354-1358. PMID: 11222296, PMCID: PMC1301327, DOI: 10.1016/s0006-3495(01)76108-5.
New Highly Hydrophobic Lewis X Glycolipids: Synthesis and Monolayer BehaviourEsnault J, Mallet J, Zhang Y, Sinaÿ P, Le Bouar T, Pincet F, Perez É. New Highly Hydrophobic Lewis X Glycolipids: Synthesis and Monolayer Behaviour. European Journal Of Organic Chemistry 2000, 2001: 253-260. DOI: 10.1002/1099-0690(200101)2001:2<253::aid-ejoc253>3.0.co;2-2.
Bilayers of neutral lipids bear a small but significant chargePincet F, Cribier S, Perez E. Bilayers of neutral lipids bear a small but significant charge. The European Physical Journal B 1999, 11: 127-130. DOI: 10.1007/bf03219162.
Bilayers of neutral lipids bear a small but significant chargePincet F, Cribier S, Perez E. Bilayers of neutral lipids bear a small but significant charge. The European Physical Journal B 1999, 11: 127. DOI: 10.1007/s100510050923.
Translational order in liquid-expanded lipid monolayers functionalized with nucleosidesPerez E, Pincet F, Goldmann M, Mioskowski C, Lebeau L. Translational order in liquid-expanded lipid monolayers functionalized with nucleosides. The European Physical Journal B 1998, 6: 1-4. DOI: 10.1007/s100510050519.
Quantitative Analysis of Holes in Supported Bilayers Providing the Adsorption Energy of Surfactants on Solid SubstrateBassereau P, Pincet F. Quantitative Analysis of Holes in Supported Bilayers Providing the Adsorption Energy of Surfactants on Solid Substrate. Langmuir 1997, 13: 7003-7007. DOI: 10.1021/la970515c.
Electrostatic Nanotitration of Weak Biochemical BondsPincet F, Rawicz W, Perez E, Lebeau L, Mioskowski C, Evans E. Electrostatic Nanotitration of Weak Biochemical Bonds. Physical Review Letters 1997, 79: 1949-1952. DOI: 10.1103/physrevlett.79.1949.
SPECIFIC FORCES BETWEEN DNA BASESPINCET F, PEREZ E, BRYANT G, LEBEAU L, MIOSKOWSKI C. SPECIFIC FORCES BETWEEN DNA BASES. Modern Physics Letters B 1996, 10: 81-99. DOI: 10.1142/s0217984996000122.
Molecular Interactions between Proteins and Synthetic Membrane Polymer FilmsPincet F, Perez E, Belfort G. Molecular Interactions between Proteins and Synthetic Membrane Polymer Films. Langmuir 1995, 11: 1229-1235. DOI: 10.1021/la00004a031.
Does Glue Contaminate the Surface Forces Apparatus?Pincet F, Perez E, Wolfe J. Does Glue Contaminate the Surface Forces Apparatus? Langmuir 1995, 11: 373-374. DOI: 10.1021/la00001a061.
Long range H-bond specific interactions between nucleosidesPincet F, Perez E, Lebeau L, Mioskowski C. Long range H-bond specific interactions between nucleosides. Physical Chemistry Chemical Physics 1995, 91: 4329-4330. DOI: 10.1039/ft9959104329.
Do Trehalose and Dimethyl Sulfoxide Affect Intermembrane Forces?Pincet F, Perez E, Wolfe J. Do Trehalose and Dimethyl Sulfoxide Affect Intermembrane Forces? Cryobiology 1994, 31: 531-539. PMID: 7835052, DOI: 10.1006/cryo.1994.1064.
Long-Range Attraction between Nucleosides with Short-Range Specificity: Direct MeasurementsPincet F, Perez E, Bryant G, Lebeau L, Mioskowski C. Long-Range Attraction between Nucleosides with Short-Range Specificity: Direct Measurements. Physical Review Letters 1994, 73: 2780-2783. PMID: 10057190, DOI: 10.1103/physrevlett.73.2780.
Do Denatured Proteins Behave Like Polymers?Pincet F, Perez E, Belfort G. Do Denatured Proteins Behave Like Polymers? Macromolecules 1994, 27: 3424-3425. DOI: 10.1021/ma00090a042.

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