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Jeff Coleman, PhD

Research Associate Cell Biology

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Selected Publications

Diacylglycerol-dependent hexamers of the SNARE-assembling chaperone Munc13-1 cooperatively bind vesiclesLi F, Grushin K, Coleman J, Pincet F, Rothman J. Diacylglycerol-dependent hexamers of the SNARE-assembling chaperone Munc13-1 cooperatively bind vesicles. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2306086120. PMID: 37883433, PMCID: PMC10623011, DOI: 10.1073/pnas.2306086120.
Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinerySundaram R, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst A, Gupta K, Rothman J, Krishnakumar S. Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2309516120. PMID: 37590407, PMCID: PMC10450444, DOI: 10.1073/pnas.2309516120.
Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin TherapyThoduvayil S, Weerakkody J, Sundaram R, Topper M, Bera M, Coleman J, Li X, Mariappan M, Ramakrishnan S. Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy. Cell Calcium 2023, 113: 102766. PMID: 37295201, PMCID: PMC10450995, DOI: 10.1016/j.ceca.2023.102766.
Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayerPanda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.
Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip (Small 51/2022)Sundaram R, Bera M, Coleman J, Weerakkody J, Krishnakumar S, Ramakrishnan S. Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip (Small 51/2022). Small 2022, 18 DOI: 10.1002/smll.202270277.
Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a ChipSundaram R, Bera M, Coleman J, Weerakkody J, Krishnakumar S, Ramakrishnan S. Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip. Small 2022, 18: e2205567. PMID: 36328714, DOI: 10.1002/smll.202205567.
A Quantitative Native Mass Spectrometry Platform for Deconstructing Hierarchical Organization of Membrane Proteins and LipidsPanda A, Giska F, Brown C, Coleman J, Rothman J, Gupta K. A Quantitative Native Mass Spectrometry Platform for Deconstructing Hierarchical Organization of Membrane Proteins and Lipids. The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.0r472.
Molecular determinants of complexin clamping and activation functionBera M, Ramakrishnan S, Coleman J, Krishnakumar SS, Rothman JE. Molecular determinants of complexin clamping and activation function. ELife 2022, 11: e71938. PMID: 35442188, PMCID: PMC9020821, DOI: 10.7554/elife.71938.
A tunable lipid bilayer native MS platform for direct determination of hierarchical organization of membrane proteins and lipids at the membranePanda A, Giska F, Brown C, Coleman J, Rothman J, Gupta K. A tunable lipid bilayer native MS platform for direct determination of hierarchical organization of membrane proteins and lipids at the membrane. Biophysical Journal 2022, 121: 312a-313a. DOI: 10.1016/j.bpj.2021.11.1192.
Vesicle capture by membrane‐bound Munc13‐1 requires self‐assembly into discrete clustersLi F, Sundaram R, Gatta AT, Coleman J, Ramakrishnan S, Krishnakumar SS, Pincet F, Rothman JE. Vesicle capture by membrane‐bound Munc13‐1 requires self‐assembly into discrete clusters. FEBS Letters 2021, 595: 2185-2196. PMID: 34227103, DOI: 10.1002/1873-3468.14157.
Nascent fusion pore opening monitored at single-SNAREpin resolutionHeo P, Coleman J, Fleury JB, Rothman JE, Pincet F. Nascent fusion pore opening monitored at single-SNAREpin resolution. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2024922118. PMID: 33495324, PMCID: PMC7865171, DOI: 10.1073/pnas.2024922118.
Munc13 binds and recruits SNAP25 to chaperone SNARE complex assemblySundaram R, Jin H, Li F, Shu T, Coleman J, Yang J, Pincet F, Zhang Y, Rothman JE, Krishnakumar SS. Munc13 binds and recruits SNAP25 to chaperone SNARE complex assembly. FEBS Letters 2020, 595: 297-309. PMID: 33222163, PMCID: PMC8068094, DOI: 10.1002/1873-3468.14006.
Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domainGruget C, Bello O, Coleman J, Krishnakumar SS, Perez E, Rothman JE, Pincet F, Donaldson SH. Synaptotagmin-1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain. Scientific Reports 2020, 10: 18011. PMID: 33093513, PMCID: PMC7581758, DOI: 10.1038/s41598-020-74923-y.
Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosisRamakrishnan S, Bera M, Coleman J, Rothman JE, Krishnakumar SS. Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosis. ELife 2020, 9: e54506. PMID: 32401194, PMCID: PMC7220375, DOI: 10.7554/elife.54506.
Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter releaseTagliatti E, Bello OD, Mendonça PRF, Kotzadimitriou D, Nicholson E, Coleman J, Timofeeva Y, Rothman JE, Krishnakumar SS, Volynski KE. Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 3819-3827. PMID: 32015138, PMCID: PMC7035618, DOI: 10.1073/pnas.1920403117.
Munc13 Clusters Capture Vesicles to Lipid Bilayer MembraneLi F, Sundaram R, Coleman J, Krishnakumar S, Pincet F, Rothman J. Munc13 Clusters Capture Vesicles to Lipid Bilayer Membrane. Biophysical Journal 2020, 118: 344a. DOI: 10.1016/j.bpj.2019.11.1990.
Dissecting the Synergistic Roles of Synaptotagmin and Complexin in Ca2+-Regulated ExocytosisRamakrishnan S, Bera M, Coleman J, Pincet F, Rothman J, Krishnakumar S. Dissecting the Synergistic Roles of Synaptotagmin and Complexin in Ca2+-Regulated Exocytosis. Biophysical Journal 2020, 118: 488a. DOI: 10.1016/j.bpj.2019.11.2702.
Munc13 Recruits SNAP25 to Facilitate SNARE Complex AssemblySundaram R, Li F, Coleman J, Pincet F, Rothman J, Krishnakumar S. Munc13 Recruits SNAP25 to Facilitate SNARE Complex Assembly. Biophysical Journal 2020, 118: 400a-401a. DOI: 10.1016/j.bpj.2019.11.2274.
Structural basis for the clamping and Ca2+ activation of SNARE-mediated fusion by synaptotagminGrushin K, Wang J, Coleman J, Rothman JE, Sindelar CV, Krishnakumar SS. Structural basis for the clamping and Ca2+ activation of SNARE-mediated fusion by synaptotagmin. Nature Communications 2019, 10: 2413. PMID: 31160571, PMCID: PMC6546687, DOI: 10.1038/s41467-019-10391-x.
Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D‐Printed Microfluidic SetupHeo P, Ramakrishnan S, Coleman J, Rothman JE, Fleury J, Pincet F. Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D‐Printed Microfluidic Setup. Small 2019, 15: e1900725. PMID: 30977975, DOI: 10.1002/smll.201900725.
Synaptotagmin oligomers are necessary and can be sufficient to form a Ca2+‐sensitive fusion clampRamakrishnan S, Bera M, Coleman J, Krishnakumar SS, Pincet F, Rothman JE. Synaptotagmin oligomers are necessary and can be sufficient to form a Ca2+‐sensitive fusion clamp. FEBS Letters 2019, 593: 154-162. PMID: 30570144, PMCID: PMC6349546, DOI: 10.1002/1873-3468.13317.
Synaptotagmin oligomerization is essential for calcium control of regulated exocytosisBello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS. Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2018, 115: e7624-e7631. PMID: 30038018, PMCID: PMC6094142, DOI: 10.1073/pnas.1808792115.
High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated AnalysisRamakrishnan S, Gohlke A, Li F, Coleman J, Xu W, Rothman JE, Pincet F. High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis. Langmuir 2018, 34: 5849-5859. PMID: 29694054, DOI: 10.1021/acs.langmuir.8b00116.
Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+Gruget C, Coleman J, Bello O, Krishnakumar SS, Perez E, Rothman JE, Pincet F, Donaldson SH. Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+. FEBS Letters 2018, 592: 1497-1506. PMID: 29578584, DOI: 10.1002/1873-3468.13040.
Structural Insight into the Interaction of Synaptotagmin-1 and Snare Complex on Lipid Bilayer by Cryo-Electron MicroscopyGrushin K, Wang J, Coleman J, Rothman J, Sindelar C, Krishnakumar S. Structural Insight into the Interaction of Synaptotagmin-1 and Snare Complex on Lipid Bilayer by Cryo-Electron Microscopy. Biophysical Journal 2018, 114: 282a. DOI: 10.1016/j.bpj.2017.11.1622.
Direct Measurement of Site-Specific Binding Energetics of Synaptotagmin-1 with Anionic Lipid MembranesGruget C, Coleman J, Bello O, Krishnakumar S, Pincet F, Rothman J, Donaldson S. Direct Measurement of Site-Specific Binding Energetics of Synaptotagmin-1 with Anionic Lipid Membranes. Biophysical Journal 2018, 114: 607a. DOI: 10.1016/j.bpj.2017.11.3318.
PRRT2 Regulates Synaptic Fusion by Directly Modulating SNARE Complex AssemblyColeman J, Jouannot O, Ramakrishnan SK, Zanetti MN, Wang J, Salpietro V, Houlden H, Rothman JE, Krishnakumar SS. PRRT2 Regulates Synaptic Fusion by Directly Modulating SNARE Complex Assembly. Cell Reports 2018, 22: 820-831. PMID: 29346777, PMCID: PMC5792450, DOI: 10.1016/j.celrep.2017.12.056.
Two Disease-Causing SNAP-25B Mutations Selectively Impair SNARE C-terminal AssemblyRebane AA, Wang B, Ma L, Qu H, Coleman J, Krishnakumar S, Rothman JE, Zhang Y. Two Disease-Causing SNAP-25B Mutations Selectively Impair SNARE C-terminal Assembly. Journal Of Molecular Biology 2017, 430: 479-490. PMID: 29056461, PMCID: PMC5805579, DOI: 10.1016/j.jmb.2017.10.012.
Correction: Re-visiting the trans insertion model for complexin clampingKrishnakumar SS, Li F, Coleman J, Schauder CM, Kümmel D, Pincet F, Rothman JE, Reinisch KM. Correction: Re-visiting the trans insertion model for complexin clamping. ELife 2017, 6: e31512. PMID: 28880147, PMCID: PMC5589411, DOI: 10.7554/elife.31512.
Synaptotagmin Interactions with Membranes: Measuring the Force of Calcium Triggering of NeurotransmissionGruget C, Coleman J, Krishankumar S, Rothman J, Pincet F, Donaldson S. Synaptotagmin Interactions with Membranes: Measuring the Force of Calcium Triggering of Neurotransmission. Biophysical Journal 2017, 112: 34a-35a. DOI: 10.1016/j.bpj.2016.11.220.
Ring-like oligomers of Synaptotagmins and related C2 domain proteinsZanetti MN, Bello OD, Wang J, Coleman J, Cai Y, Sindelar CV, Rothman JE, Krishnakumar SS. Ring-like oligomers of Synaptotagmins and related C2 domain proteins. ELife 2016, 5: e17262. PMID: 27434670, PMCID: PMC4977156, DOI: 10.7554/elife.17262.
Re-visiting the trans insertion model for complexin clampingKrishnakumar SS, Li F, Coleman J, Schauder CM, Kümmel D, Pincet F, Rothman JE, Reinisch KM. Re-visiting the trans insertion model for complexin clamping. ELife 2015, 4: e04463. PMID: 25831964, PMCID: PMC4384536, DOI: 10.7554/elife.04463.
Calcium sensitive ring-like oligomers formed by synaptotagminWang J, Bello O, Auclair SM, Wang J, Coleman J, Pincet F, Krishnakumar SS, Sindelar CV, Rothman JE. Calcium sensitive ring-like oligomers formed by synaptotagmin. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 13966-13971. PMID: 25201968, PMCID: PMC4183308, DOI: 10.1073/pnas.1415849111.
Genetic analysis of the Complexin trans-clamping model for cross-linking SNARE complexes in vivoCho RW, Kümmel D, Li F, Baguley SW, Coleman J, Rothman JE, Littleton JT. Genetic analysis of the Complexin trans-clamping model for cross-linking SNARE complexes in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 10317-10322. PMID: 24982161, PMCID: PMC4104896, DOI: 10.1073/pnas.1409311111.
A Half-Zippered SNARE Complex Represents a Functional Intermediate in Membrane FusionLi F, Kümmel D, Coleman J, Reinisch KM, Rothman JE, Pincet F. A Half-Zippered SNARE Complex Represents a Functional Intermediate in Membrane Fusion. Journal Of The American Chemical Society 2014, 136: 3456-3464. PMID: 24533674, PMCID: PMC3985920, DOI: 10.1021/ja410690m.
Common intermediates and kinetics, but different energetics, in the assembly of SNARE proteinsZorman S, Rebane AA, Ma L, Yang G, Molski MA, Coleman J, Pincet F, Rothman JE, Zhang Y. Common intermediates and kinetics, but different energetics, in the assembly of SNARE proteins. ELife 2014, 3: e03348. PMID: 25180101, PMCID: PMC4166003, DOI: 10.7554/elife.03348.
FisB Mediated Membrane Fission During Sporulation in Bacillus SubtilisBraun M, Rodrigues C, Doan T, Coleman J, Rudner D, Karatekin E. FisB Mediated Membrane Fission During Sporulation in Bacillus Subtilis. Biophysical Journal 2014, 106: 524a. DOI: 10.1016/j.bpj.2013.11.2923.
FisB mediates membrane fission during sporulation in Bacillus subtilisDoan T, Coleman J, Marquis KA, Meeske AJ, Burton BM, Karatekin E, Rudner DZ. FisB mediates membrane fission during sporulation in Bacillus subtilis. Genes & Development 2013, 27: 322-334. PMID: 23388828, PMCID: PMC3576517, DOI: 10.1101/gad.209049.112.
Dual roles of Munc18-1 rely on distinct binding modes of the central cavity with Stx1A and SNARE complexShi L, Kümmel D, Coleman J, Melia TJ, Giraudo CG. Dual roles of Munc18-1 rely on distinct binding modes of the central cavity with Stx1A and SNARE complex. Molecular Biology Of The Cell 2011, 22: 4150-4160. PMID: 21900493, PMCID: PMC3204075, DOI: 10.1091/mbc.e11-02-0150.
A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusionKrishnakumar SS, Radoff DT, Kümmel D, Giraudo CG, Li F, Khandan L, Baguley SW, Coleman J, Reinisch KM, Pincet F, Rothman JE. A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusion. Nature Structural & Molecular Biology 2011, 18: 934-940. PMID: 21785412, PMCID: PMC3668341, DOI: 10.1038/nsmb.2103.
Protein Determinants of SNARE-Mediated Lipid MixingJi H, Coleman J, Yang R, Melia TJ, Rothman JE, Tareste D. Protein Determinants of SNARE-Mediated Lipid Mixing. Biophysical Journal 2010, 99: 553-560. PMID: 20643074, PMCID: PMC2905075, DOI: 10.1016/j.bpj.2010.04.060.
Phosphatidylinositol 4-Phosphate Controls Both Membrane Recruitment and a Regulatory Switch of the Rab GEF Sec2pMizuno-Yamasaki E, Medkova M, Coleman J, Novick P. Phosphatidylinositol 4-Phosphate Controls Both Membrane Recruitment and a Regulatory Switch of the Rab GEF Sec2p. Developmental Cell 2010, 18: 828-840. PMID: 20493815, PMCID: PMC2877039, DOI: 10.1016/j.devcel.2010.03.016.
A fast, single-vesicle fusion assay mimics physiological SNARE requirementsKaratekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, Rothman JE. A fast, single-vesicle fusion assay mimics physiological SNARE requirements. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 3517-3521. PMID: 20133592, PMCID: PMC2840481, DOI: 10.1073/pnas.0914723107.
A Fast, Single-Vesicle Fusion Assay Mimics Physiological SNARE RequirementsKaratekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, Rothman J. A Fast, Single-Vesicle Fusion Assay Mimics Physiological SNARE Requirements. Biophysical Journal 2010, 98: 616a. DOI: 10.1016/j.bpj.2009.12.3362.
An Internal Domain of Exo70p Is Required for Actin-independent Localization and Mediates Assembly of Specific Exocyst ComponentsHutagalung A, Coleman J, Pypaert M, Novick P. An Internal Domain of Exo70p Is Required for Actin-independent Localization and Mediates Assembly of Specific Exocyst Components. Molecular Biology Of The Cell 2008, 20: 153-163. PMID: 18946089, PMCID: PMC2613103, DOI: 10.1091/mbc.e08-02-0157.
Rtn1p Is Involved in Structuring the Cortical Endoplasmic ReticulumDe Craene J, Coleman J, Estrada de Martin P, Pypaert M, Anderson S, Yates J, Ferro-Novick S, Novick P. Rtn1p Is Involved in Structuring the Cortical Endoplasmic Reticulum. Molecular Biology Of The Cell 2006, 17: 3009-3020. PMID: 16624861, PMCID: PMC1483037, DOI: 10.1091/mbc.e06-01-0080.
The rab Exchange Factor Sec2p Reversibly Associates with the ExocystMedkova M, France Y, Coleman J, Novick P. The rab Exchange Factor Sec2p Reversibly Associates with the Exocyst. Molecular Biology Of The Cell 2006, 17: 2757-2769. PMID: 16611746, PMCID: PMC1474791, DOI: 10.1091/mbc.e05-10-0917.
The polarity-establishment component Bem1p interacts with the exocyst complex through the Sec15p subunitFrance Y, Boyd C, Coleman J, Novick P. The polarity-establishment component Bem1p interacts with the exocyst complex through the Sec15p subunit. Journal Of Cell Science 2006, 119: 876-888. PMID: 16478783, DOI: 10.1242/jcs.02849.
Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiaeEstrada P, Kim J, Coleman J, Walker L, Dunn B, Takizawa P, Novick P, Ferro-Novick S. Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae. Journal Of Cell Biology 2003, 163: 1255-1266. PMID: 14691136, PMCID: PMC2173705, DOI: 10.1083/jcb.200304030.

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