TuKiet Lam, PhD, BA
Research & Publications
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Research Summary
Specialized keywords: Proteomics; mass spectrometry, cancer research
Extensive Research Description
I am the Director of the Keck MS & Proteomics Resource at the Yale School of Medicine and have been with Yale since 2004. My specific aims are to train, assist, collaborate, and make available my expertise in mass spectrometry to as many investigators of other scientific and biomedical discipline as possible so that they can advance their research. By establishing a well-organized and well-trained core group of staffs who are dedicated to the same philosophy and mission, my team provides the high-quality mass spectrometry data and analyses which have resulted in co-authorships and acknowledgements in many high impact scientific publications. My mass spectrometry (MS) footing grew from my graduate career at Florida State University, where I developed a hydrogen/deuterium exchange workflow to robustly observe protein-protein interaction(s) and structural changes in proteins. The technique led to the discovery of unique conformational changes in HIV-1 viral capsid protein (Lanman, et. al., JMB, 325, 759-772, 2003, Cover). I became involved in many collaborative projects during my postdoctoral work at the National High Magnetic Field Lab and began to shape my career in using my analytical chemistry skillsets in high resolution tandem mass spectrometry to work with a multitude of investigators across many scientific and biomedical disciplines to understand biological functions at the molecular level. My successful multidisciplinary collaborative interaction with these investigators falls in the areas of protein identification, profiling, and quantitation; protein posttranslational modification (PTM); protein-protein interactions; natural products; metabolites; green chemistry; and organic product syntheses among many others. During my residence within the Keck MS and Proteomics Resource, I collaborated many biomedical and clinical investigators in their aims to better understand protein(s) functions in various relevant disease and cancers studies such as illustrated in Fogel et al, 2010, (which was featured on the cover of J. Biol. Chem. 285, 34864); Musante et al, 2017 (Elife 6); Miller et al, 2017 (ACS Chem Neurosci, 8(7), 1554); Sun et al, 2016 (Arthritis Rheumatology, 68(5), 1252); Tellez et al, 2013 (Sciene 341(6147), 800); Klein et al, 2017 (Neruon 95, p281); Goffredo et al, 2017 (Nutrients, 9, p 642); and Henderson et al, 2016 (Acta Neuropathol 131, p621). Currently I am involved in many projects which utilizes high resolution mass spectrometry (Orbitrap family of mass spectrometers) Label Free Quantitative LC MS and LC MS/MS methodology for protein ID/profiling and quantitation in various biological systems (i.e. brain, serum, plasma, liver, endothelial cells, FACS sorted cells, neurons, worms, mosquitos, flies, etc.), for protein ubiquitinylation, palmitoylation, acetylation, and phosphorylation modification, for small molecule quantitation and structural elucidation along with targeted drug compounds assays. The use of these exceedingly more reproducible high resolution mass spectrometry to facilitate protein and protein posttranslational modification ID and quantitative global profiling provides a very robust and biostatistical confident way to monitor protein changes, functions, and interactions within a complex biological system.
Coauthors
Research Interests
Chromatography, High Pressure Liquid; Lipids; Metabolism; Peptide Mapping; Peptides; Proteins; Research Design; Mass Spectrometry; Tissue Extracts; Spectrometry, Mass, Electrospray Ionization; Proteomics; Tandem Mass Spectrometry
Public Health Interests
Aging; Biomarkers; Cancer; Cardiovascular Diseases; Respiratory Disease/Infections
Research Image
Proteomics Workflow
Selected Publications
- Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson’s diseaseVidyadhara D, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam T, Sames D, Greene L, Sulzer D, Chandra S. Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson’s disease Cell Reports 2023, 42: 112231. PMID: 36920906, DOI: 10.1016/j.celrep.2023.112231.
- The choroid plexus links innate immunity to CSF dysregulation in hydrocephalusRobert S, Reeves B, Kiziltug E, Duy P, Karimy J, Mansuri M, Marlier A, Allington G, Greenberg A, DeSpenza T, Singh A, Zeng X, Mekbib K, Kundishora A, Nelson-Williams C, Hao L, Zhang J, Lam T, Wilson R, Butler W, Diluna M, Feinberg P, Schafer D, Movahedi K, Tannenbaum A, Koundal S, Chen X, Benveniste H, Limbrick D, Schiff S, Carter B, Gunel M, Simard J, Lifton R, Alper S, Delpire E, Kahle K. The choroid plexus links innate immunity to CSF dysregulation in hydrocephalus Cell 2023, 186: 764-785.e21. PMID: 36803604, DOI: 10.1016/j.cell.2023.01.017.
- Proteome-wide screening for mitogen-activated protein kinase docking motifs and interactorsShi G, Song C, Torres Robles J, Salichos L, Lou H, Lam T, Gerstein M, Turk B. Proteome-wide screening for mitogen-activated protein kinase docking motifs and interactors Science Signaling 2023, 16: eabm5518. PMID: 36626580, PMCID: PMC9995140, DOI: 10.1126/scisignal.abm5518.
- Applying Data‐Independent Acquisition Mass Spectrometry on Cerebrospinal Fluid Samples from a Neurology Clinic for Biomarker Discovery in Alzheimer’s Diseasede Geus M, Leslie S, Ramirez C, Trombetta B, Wang W, Lam T, Gotti C, Roux‐Dalvai F, Droit A, Nairn A, Arnold S, Carlyle B. Applying Data‐Independent Acquisition Mass Spectrometry on Cerebrospinal Fluid Samples from a Neurology Clinic for Biomarker Discovery in Alzheimer’s Disease Alzheimer's & Dementia 2022, 18 DOI: 10.1002/alz.064314.
- T124. PERIPHERAL SIGNATURE OF ALTERED SYNAPTIC INTEGRITY IN YOUNG ONSET CANNABIS USE DISORDER: AN EXPLORATORY PROTEOMIC STUDY OF CIRCULATING EXTRACELLULAR VESICLESGanesh S, Lam T, Nairn A, Milian R, Eitan E, Ranganathan M. T124. PERIPHERAL SIGNATURE OF ALTERED SYNAPTIC INTEGRITY IN YOUNG ONSET CANNABIS USE DISORDER: AN EXPLORATORY PROTEOMIC STUDY OF CIRCULATING EXTRACELLULAR VESICLES European Neuropsychopharmacology 2022, 63: e235-e236. DOI: 10.1016/j.euroneuro.2022.07.420.
- Proteomic profiling reveals an association between ALDH and oxidative phosphorylation and DNA damage repair pathways in human colon adenocarcinoma stem cellsWang Y, Chen Y, Garcia-Milian R, Golla JP, Charkoftaki G, Lam TT, Thompson DC, Vasiliou V. Proteomic profiling reveals an association between ALDH and oxidative phosphorylation and DNA damage repair pathways in human colon adenocarcinoma stem cells Chemico-Biological Interactions 2022, 368: 110175. PMID: 36162455, PMCID: PMC9891852, DOI: 10.1016/j.cbi.2022.110175.
- Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproductionGriffin KN, Walters BW, Li H, Wang H, Biancon G, Tebaldi T, Kaya CB, Kanyo J, Lam TT, Cox AL, Halene S, Chung JJ, Lesch BJ. Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproduction Genome Research 2022, 32: 1655-1668. PMID: 36109149, PMCID: PMC9528986, DOI: 10.1101/gr.276578.122.
- Autoinhibition of the GEF activity of cytoskeletal regulatory protein Trio is disrupted in neurodevelopmental disorder-related genetic variantsBircher JE, Corcoran EE, Lam TT, Trnka MJ, Koleske AJ. Autoinhibition of the GEF activity of cytoskeletal regulatory protein Trio is disrupted in neurodevelopmental disorder-related genetic variants Journal Of Biological Chemistry 2022, 298: 102361. PMID: 35963430, PMCID: PMC9467883, DOI: 10.1016/j.jbc.2022.102361.
- LB901 Inflammaging in human skin: Early onset of senescence and imbalanced epidermal homeostasis across the decadesOblong J, Jarrold B, Tan C, Ho C, Soon A, Lam T, Yang X, Nguyen C, Guo W, Chew Y, DeAngelis Y, Costello L, De Los Santos Gomez P, Przyborski S, Bellanger S, Dreesen O, Kimball A. LB901 Inflammaging in human skin: Early onset of senescence and imbalanced epidermal homeostasis across the decades Journal Of Investigative Dermatology 2022, 142: b9. DOI: 10.1016/j.jid.2022.05.919.
- Identification of growth hormone receptor as a relevant target for precision medicine in low‐EGFR expressing glioblastomaVerreault M, Vilchis I, Rosenberg S, Lemaire N, Schmitt C, Guehennec J, Royer‐Perron L, Thomas J, Lam TT, Dingli F, Loew D, Ducray F, Paris S, Carpentier C, Marie Y, Laigle‐Donadey F, Rousseau A, Pigat N, Boutillon F, Bielle F, Mokhtari K, Frank SJ, Reyniès A, Hoang‐Xuan K, Sanson M, Goffin V, Idbaih A. Identification of growth hormone receptor as a relevant target for precision medicine in low‐EGFR expressing glioblastoma Clinical And Translational Medicine 2022, 12: e939. PMID: 35808822, PMCID: PMC9270581, DOI: 10.1002/ctm2.939.
- Carbonyl Posttranslational Modification Associated With Early-Onset Type 1 Diabetes Autoimmunity.Yang ML, Connolly SE, Gee RJ, Lam TT, Kanyo J, Peng J, Guyer P, Syed F, Tse HM, Clarke SG, Clarke CF, James EA, Speake C, Evans-Molina C, Arvan P, Herold KC, Wen L, Mamula MJ. Carbonyl Posttranslational Modification Associated With Early-Onset Type 1 Diabetes Autoimmunity. Diabetes 2022, 71: 1979-1993. PMID: 35730902, PMCID: PMC9450849, DOI: 10.2337/db21-0989.
- Proteomic characterization of post-mortem human brain tissue following ultracentrifugation-based subcellular fractionationKandigian SE, Ethier EC, Kitchen RR, Lam TT, Arnold SE, Carlyle BC. Proteomic characterization of post-mortem human brain tissue following ultracentrifugation-based subcellular fractionation Brain Communications 2022, 4: fcac103-. PMID: 35611312, PMCID: PMC9123841, DOI: 10.1093/braincomms/fcac103.
- Citrullination of glucokinase is linked to autoimmune diabetesYang ML, Horstman S, Gee R, Guyer P, Lam TT, Kanyo J, Perdigoto AL, Speake C, Greenbaum CJ, Callebaut A, Overbergh L, Kibbey RG, Herold KC, James EA, Mamula MJ. Citrullination of glucokinase is linked to autoimmune diabetes Nature Communications 2022, 13: 1870. PMID: 35388005, PMCID: PMC8986778, DOI: 10.1038/s41467-022-29512-0.
- Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic functionGorenberg EL, Tieze S, Yücel B, Zhao HR, Chou V, Wirak GS, Tomita S, Lam TT, Chandra SS. Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function PLOS Biology 2022, 20: e3001590. PMID: 35358180, PMCID: PMC9004782, DOI: 10.1371/journal.pbio.3001590.
- Mycobacterium tuberculosis encodes a YhhN family membrane protein with lysoplasmalogenase activity that protects against toxic host lysolipidsJurkowitz MS, Azad AK, Monsma PC, Keiser TL, Kanyo J, Lam TT, Bell CE, Schlesinger LS. Mycobacterium tuberculosis encodes a YhhN family membrane protein with lysoplasmalogenase activity that protects against toxic host lysolipids Journal Of Biological Chemistry 2022, 298: 101849. PMID: 35314194, PMCID: PMC9052158, DOI: 10.1016/j.jbc.2022.101849.
- Sex- and estrous-cycle dependent dorsal hippocampal phosphoproteomic changes induced by low-dose ketamineSaland SK, Wilczak K, Voss E, Lam TT, Kabbaj M. Sex- and estrous-cycle dependent dorsal hippocampal phosphoproteomic changes induced by low-dose ketamine Scientific Reports 2022, 12: 1820. PMID: 35110693, PMCID: PMC8810966, DOI: 10.1038/s41598-022-05937-x.
- Characterization of temporospatial distribution of renal tubular casts by nephron tracking after ischemia-reperfusion injuryShin NS, Marlier A, Xu L, Lam T, Cantley LG, Guo JK. Characterization of temporospatial distribution of renal tubular casts by nephron tracking after ischemia-reperfusion injury American Journal Of Physiology. Renal Physiology 2022, 322: f322-f334. PMID: 35100823, PMCID: PMC8897010, DOI: 10.1152/ajprenal.00284.2021.
- Simple, Single-Shot Phosphoproteomic Analysis of Heat-Stable Tau Identifies Age-Related Changes in pS235- and pS396-Tau Levels in Non-human PrimatesLeslie SN, Kanyo J, Datta D, Wilson RS, Zeiss C, Duque A, Lam TT, Arnsten AFT, Nairn AC. Simple, Single-Shot Phosphoproteomic Analysis of Heat-Stable Tau Identifies Age-Related Changes in pS235- and pS396-Tau Levels in Non-human Primates Frontiers In Aging Neuroscience 2021, 13: 767322. PMID: 34867294, PMCID: PMC8637411, DOI: 10.3389/fnagi.2021.767322.
- Liver-Dependent Lung Remodeling during Systemic Inflammation Shapes Responses to Secondary Infection.Odom CV, Kim Y, Burgess CL, Baird LA, Korkmaz FT, Na E, Shenoy AT, Arafa EI, Lam TT, Jones MR, Mizgerd JP, Traber KE, Quinton LJ. Liver-Dependent Lung Remodeling during Systemic Inflammation Shapes Responses to Secondary Infection. The Journal Of Immunology 2021, 207: 1891-1902. PMID: 34470857, PMCID: PMC8631467, DOI: 10.4049/jimmunol.2100254.
- Cocaine self-administration induces sex-dependent protein expression in the nucleus accumbensLópez AJ, Johnson AR, Euston TJ, Wilson R, Nolan SO, Brady LJ, Thibeault KC, Kelly SJ, Kondev V, Melugin P, Kutlu MG, Chuang E, Lam TT, Kiraly DD, Calipari ES. Cocaine self-administration induces sex-dependent protein expression in the nucleus accumbens Communications Biology 2021, 4: 883. PMID: 34272455, PMCID: PMC8285523, DOI: 10.1038/s42003-021-02358-w.
- Sex Differences in the Ventral Tegmental Area and Nucleus Accumbens Proteome at Baseline and Following Nicotine ExposureLee AM, Mansuri MS, Wilson RS, Lam TT, Nairn AC, Picciotto MR. Sex Differences in the Ventral Tegmental Area and Nucleus Accumbens Proteome at Baseline and Following Nicotine Exposure Frontiers In Molecular Neuroscience 2021, 14: 657064. PMID: 34335180, PMCID: PMC8317211, DOI: 10.3389/fnmol.2021.657064.
- Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertensionSitapara R, Lam TT, Gandjeva A, Tuder RM, Zisman LS. Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension Pulmonary Circulation 2021, 11: 1-15. PMID: 34966541, PMCID: PMC8711668, DOI: 10.1177/20458940211031109.
- Regulation of Immune Cell Matrix Metalloproteinase Production by the Sarcoidosis Lung MatrisomeLiu A, Sun H, Winkler J, Reeves B, Gulati M, Wang W, Lam T, Herzog E. Regulation of Immune Cell Matrix Metalloproteinase Production by the Sarcoidosis Lung Matrisome 2021, a4262-a4262. DOI: 10.1164/ajrccm-conference.2021.203.1_meetingabstracts.a4262.
- Small extracellular vesicles control dendritic spine development through regulation of HDAC2 signalingZhang L, Lin TV, Yuan Q, Sadoul R, Lam TT, Bordey A. Small extracellular vesicles control dendritic spine development through regulation of HDAC2 signaling Journal Of Neuroscience 2021, 41: jn-rm-0766-20. PMID: 33741723, PMCID: PMC8084316, DOI: 10.1523/jneurosci.0766-20.2021.
- Granulocyte-Colony Stimulating Factor Reduces Cocaine-Seeking and Downregulates Glutamatergic Synaptic Proteins in Medial Prefrontal CortexHofford RS, Euston TJ, Wilson RS, Meckel KR, Peck EG, Godino A, Landry JA, Calipari ES, Lam TT, Kiraly DD. Granulocyte-Colony Stimulating Factor Reduces Cocaine-Seeking and Downregulates Glutamatergic Synaptic Proteins in Medial Prefrontal Cortex Journal Of Neuroscience 2020, 41: 1553-1565. PMID: 33361463, PMCID: PMC7896012, DOI: 10.1523/jneurosci.1452-20.2020.
- Differential Protein Expression in Striatal D1- and D2-Dopamine Receptor-Expressing Medium Spiny NeuronsMansuri MS, Peng G, Wilson RS, Lam TT, Zhao H, Williams KR, Nairn AC. Differential Protein Expression in Striatal D1- and D2-Dopamine Receptor-Expressing Medium Spiny Neurons Proteomes 2020, 8: 27. PMID: 33066078, PMCID: PMC7709116, DOI: 10.3390/proteomes8040027.
- Bioactive Plasma Mitochondrial DNA Is Associated With Disease Progression in Scleroderma‐Associated Interstitial Lung DiseaseRyu C, Walia A, Ortiz V, Perry C, Woo S, Reeves BC, Sun H, Winkler J, Kanyo JE, Wang W, Vukmirovic M, Ristic N, Stratton EA, Meena SR, Minasyan M, Kurbanov D, Liu X, Lam TT, Farina G, Gomez JL, Gulati M, Herzog EL. Bioactive Plasma Mitochondrial DNA Is Associated With Disease Progression in Scleroderma‐Associated Interstitial Lung Disease Arthritis & Rheumatology 2020, 72: 1905-1915. PMID: 32602227, PMCID: PMC8081728, DOI: 10.1002/art.41418.
- Cerebrospinal fluid proteome evaluation in major depressive disorder by mass spectrometryFranzen AD, Lam TT, Williams KR, Nairn AC, Duman RS, Sathyanesan M, Kumar V, Carpenter LL, Newton SS. Cerebrospinal fluid proteome evaluation in major depressive disorder by mass spectrometry BMC Psychiatry 2020, 20: 481. PMID: 32998701, PMCID: PMC7528485, DOI: 10.1186/s12888-020-02874-9.
- Itaconate is an effector of a Rab GTPase cell-autonomous host defense pathway against SalmonellaChen M, Sun H, Boot M, Shao L, Chang SJ, Wang W, Lam TT, Lara-Tejero M, Rego EH, Galán JE. Itaconate is an effector of a Rab GTPase cell-autonomous host defense pathway against Salmonella Science 2020, 369: 450-455. PMID: 32703879, PMCID: PMC8020367, DOI: 10.1126/science.aaz1333.
- Glucocorticoids and serum- and glucocorticoid-inducible kinase 1 are potent regulators of CFTR in the native intestine: implications for stress-induced diarrheaAhsan MK, Figueroa-Hall L, Baratta V, Garcia-Milian R, Lam TT, Hoque K, Salas PJ, Ameen NA. Glucocorticoids and serum- and glucocorticoid-inducible kinase 1 are potent regulators of CFTR in the native intestine: implications for stress-induced diarrhea AJP Gastrointestinal And Liver Physiology 2020, 319: g121-g132. PMID: 32567324, PMCID: PMC7500270, DOI: 10.1152/ajpgi.00076.2020.
- Sarcoidosis Extracellular Matrix Regulation of Immune Cell PhenotypeLiu A, Sun H, Ledesma-Mendoza A, Winkler J, Reeves B, Gulati M, Wang W, Lam T, Herzog E. Sarcoidosis Extracellular Matrix Regulation of Immune Cell Phenotype 2020, a5530-a5530. DOI: 10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a5530.
- An omic approach to congenital diaphragmatic hernia: a pilot study of genomic, microRNA, and metabolomic profilingPiersigilli F, Syed M, Lam TT, Dotta A, Massoud M, Vernocchi P, Quagliariello A, Putignani L, Auriti C, Salvatori G, Bagolan P, Bhandari V. An omic approach to congenital diaphragmatic hernia: a pilot study of genomic, microRNA, and metabolomic profiling Journal Of Perinatology 2020, 40: 952-961. PMID: 32080334, DOI: 10.1038/s41372-020-0623-3.
- Nutrient Composition and Fatty Acid and Protein Profiles of Selected Fish By-ProductsKandyliari A, Mallouchos A, Papandroulakis N, Golla JP, Lam TT, Sakellari A, Karavoltsos S, Vasiliou V, Kapsokefalou M. Nutrient Composition and Fatty Acid and Protein Profiles of Selected Fish By-Products Foods 2020, 9: 190. PMID: 32075005, PMCID: PMC7074476, DOI: 10.3390/foods9020190.
- Phosphorylation of human placental aromatase CYP19A1.Ghosh D, Egbuta C, Kanyo J, Lam TT. Phosphorylation of human placental aromatase CYP19A1. Biochemical Journal 2019, 476: 3313-3331. PMID: 31652308, PMCID: PMC7069221, DOI: 10.1042/bcj20190633.
- A single phosphoacceptor residue in BGLF3 is essential for transcription of Epstein-Barr virus late genesLi J, Walsh A, Lam TT, Delecluse HJ, El-Guindy A. A single phosphoacceptor residue in BGLF3 is essential for transcription of Epstein-Barr virus late genes PLOS Pathogens 2019, 15: e1007980. PMID: 31461506, PMCID: PMC6713331, DOI: 10.1371/journal.ppat.1007980.
- Preincubation With Everolimus and Sirolimus Reduces Organic Anion-Transporting Polypeptide (OATP)1B1- and 1B3-Mediated Transport Independently of mTOR Kinase Inhibition: Implication in Assessing OATP1B1- and OATP1B3-Mediated Drug-Drug InteractionsFarasyn T, Crowe A, Hatley O, Neuhoff S, Alam K, Kanyo J, Lam T, Ding K, Yue W. Preincubation With Everolimus and Sirolimus Reduces Organic Anion-Transporting Polypeptide (OATP)1B1- and 1B3-Mediated Transport Independently of mTOR Kinase Inhibition: Implication in Assessing OATP1B1- and OATP1B3-Mediated Drug-Drug Interactions Journal Of Pharmaceutical Sciences 2019, 108: 3443-3456. PMID: 31047942, PMCID: PMC6759397, DOI: 10.1016/j.xphs.2019.04.019.
- Development of Targeted Mass Spectrometry-Based Approaches for Quantitation of Proteins Enriched in the Postsynaptic Density (PSD)Wilson RS, Rauniyar N, Sakaue F, Lam TT, Williams KR, Nairn AC. Development of Targeted Mass Spectrometry-Based Approaches for Quantitation of Proteins Enriched in the Postsynaptic Density (PSD) Proteomes 2019, 7: 12. PMID: 30986977, PMCID: PMC6630806, DOI: 10.3390/proteomes7020012.
- Integrated multi-omics approach reveals a role of ALDH1A1 in lipid metabolism in human colon cancer cellsCharkoftaki G, Thompson DC, Golla JP, Garcia-Milian R, Lam TT, Engel J, Vasiliou V. Integrated multi-omics approach reveals a role of ALDH1A1 in lipid metabolism in human colon cancer cells Chemico-Biological Interactions 2019, 304: 88-96. PMID: 30851239, PMCID: PMC7988342, DOI: 10.1016/j.cbi.2019.02.030.
- Synovial fluid proteome changes in ACL injury-induced posttraumatic osteoarthritis: Proteomics analysis of porcine knee synovial fluidKiapour AM, Sieker JT, Proffen BL, Lam TT, Fleming BC, Murray MM. Synovial fluid proteome changes in ACL injury-induced posttraumatic osteoarthritis: Proteomics analysis of porcine knee synovial fluid PLOS ONE 2019, 14: e0212662. PMID: 30822327, PMCID: PMC6396923, DOI: 10.1371/journal.pone.0212662.
- Identification of new biomarkers of bronchopulmonary dysplasia using metabolomicsPiersigilli F, Lam TT, Vernocchi P, Quagliariello A, Putignani L, Aghai ZH, Bhandari V. Identification of new biomarkers of bronchopulmonary dysplasia using metabolomics Metabolomics 2019, 15: 20. PMID: 30830433, DOI: 10.1007/s11306-019-1482-9.
- Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft ProteinsCijsouw T, Ramsey AM, Lam TT, Carbone BE, Blanpied TA, Biederer T. Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins Proteomes 2018, 6: 48. PMID: 30487426, PMCID: PMC6313906, DOI: 10.3390/proteomes6040048.
- ProteomicsBrowser: MS/proteomics data visualization and investigation.Peng G, Wilson R, Tang Y, Lam TT, Nairn AC, Williams K, Zhao H. ProteomicsBrowser: MS/proteomics data visualization and investigation. Bioinformatics 2018, 35: 2313-2314. PMID: 30462190, PMCID: PMC6596887, DOI: 10.1093/bioinformatics/bty958.
- Phosphoproteomic analysis of cocaine memory extinction and reconsolidation in the nucleus accumbensTorregrossa MM, MacDonald M, Stone KL, Lam TT, Nairn AC, Taylor JR. Phosphoproteomic analysis of cocaine memory extinction and reconsolidation in the nucleus accumbens Psychopharmacology 2018, 236: 531-543. PMID: 30411139, PMCID: PMC6374162, DOI: 10.1007/s00213-018-5071-9.
- Development and Validation of LC–MS-MS Assay for the Determination of the Emerging Alkylating Agent Laromustine and Its Active Metabolite in Human PlasmaNassar AF, Wisnewski AV, Wu T, Lam TT, King I. Development and Validation of LC–MS-MS Assay for the Determination of the Emerging Alkylating Agent Laromustine and Its Active Metabolite in Human Plasma Journal Of Chromatographic Science 2018, 57: 195-203. PMID: 30395213, DOI: 10.1093/chromsci/bmy100.
- Evaluation of the Phosphoproteome of Mouse Alpha 4/Beta 2-Containing Nicotinic Acetylcholine Receptors In Vitro and In VivoMiller MB, Wilson RS, Lam TT, Nairn AC, Picciotto MR. Evaluation of the Phosphoproteome of Mouse Alpha 4/Beta 2-Containing Nicotinic Acetylcholine Receptors In Vitro and In Vivo Proteomes 2018, 6: 42. PMID: 30326594, PMCID: PMC6313896, DOI: 10.3390/proteomes6040042.
- Phosphoproteomic Analysis of the Amygdala Response to Adolescent Glucocorticoid Exposure Reveals G-Protein Coupled Receptor Kinase 2 as a Target for Reducing Motivation for AlcoholBertholomey ML, Stone K, Lam TT, Bang S, Wu W, Nairn AC, Taylor JR, Torregrossa MM. Phosphoproteomic Analysis of the Amygdala Response to Adolescent Glucocorticoid Exposure Reveals G-Protein Coupled Receptor Kinase 2 as a Target for Reducing Motivation for Alcohol Proteomes 2018, 6: 41. PMID: 30322021, PMCID: PMC6313880, DOI: 10.3390/proteomes6040041.
- Granulocyte-Colony-Stimulating Factor Alters the Proteomic Landscape of the Ventral Tegmental AreaMervosh NL, Wilson R, Rauniyar N, Hofford RS, Kutlu MG, Calipari ES, Lam TT, Kiraly DD. Granulocyte-Colony-Stimulating Factor Alters the Proteomic Landscape of the Ventral Tegmental Area Proteomes 2018, 6: 35. PMID: 30249060, PMCID: PMC6313867, DOI: 10.3390/proteomes6040035.
- Isoform-Level Interpretation of High-Throughput Proteomics Data Enabled by Deep Integration with RNA-seqCarlyle B, Kitchen RR, Zhang J, Wilson R, Lam T, Rozowsky JS, Williams KR, Sestan N, Gerstein M, Nairn AC. Isoform-Level Interpretation of High-Throughput Proteomics Data Enabled by Deep Integration with RNA-seq Journal Of Proteome Research 2018, 17: 3431-3444. PMID: 30125121, PMCID: PMC6392456, DOI: 10.1021/acs.jproteome.8b00310.
- Global phosphoproteomic analysis identifies SRMS-regulated secondary signaling intermediatesGoel RK, Meyer M, Paczkowska M, Reimand J, Vizeacoumar F, Vizeacoumar F, Lam TT, Lukong KE. Global phosphoproteomic analysis identifies SRMS-regulated secondary signaling intermediates Proteome Science 2018, 16: 16. PMID: 30140170, PMCID: PMC6098843, DOI: 10.1186/s12953-018-0143-7.
- Quantification of Urinary Protein Biomarkers of Autosomal Dominant Polycystic Kidney Disease by Parallel Reaction MonitoringRauniyar N, Yu X, Cantley J, Voss EZ, Belcher J, Colangelo CM, Stone KL, Dahl N, Parikh C, Lam TT, Cantley LG. Quantification of Urinary Protein Biomarkers of Autosomal Dominant Polycystic Kidney Disease by Parallel Reaction Monitoring Proteomics Clinical Applications 2018, 12: e1700157. PMID: 29573172, PMCID: PMC6736530, DOI: 10.1002/prca.201700157.
- The repeat region of cortactin is intrinsically disordered in solutionLi X, Tao Y, Murphy JW, Scherer AN, Lam TT, Marshall AG, Koleske AJ, Boggon TJ. The repeat region of cortactin is intrinsically disordered in solution Scientific Reports 2017, 7: 16696. PMID: 29196701, PMCID: PMC5711941, DOI: 10.1038/s41598-017-16959-1.
- MELK Promotes Melanoma Growth by Stimulating the NF-κB PathwayJanostiak R, Rauniyar N, Lam TT, Ou J, Zhu LJ, Green MR, Wajapeyee N. MELK Promotes Melanoma Growth by Stimulating the NF-κB Pathway Cell Reports 2017, 21: 2829-2841. PMID: 29212029, PMCID: PMC5726781, DOI: 10.1016/j.celrep.2017.11.033.
- A multiregional proteomic survey of the postnatal human brainCarlyle BC, Kitchen RR, Kanyo JE, Voss EZ, Pletikos M, Sousa AMM, Lam TT, Gerstein MB, Sestan N, Nairn AC. A multiregional proteomic survey of the postnatal human brain Nature Neuroscience 2017, 20: 1787-1795. PMID: 29184206, PMCID: PMC5894337, DOI: 10.1038/s41593-017-0011-2.
- 6 Differential Redox Changes in Liver Mitochondrial Proteomes from Phenotypically Distinct Mouse Models of Glutathione DeficiencyChen Y, Lam T, Yu X, Vasiliou V. 6 Differential Redox Changes in Liver Mitochondrial Proteomes from Phenotypically Distinct Mouse Models of Glutathione Deficiency Free Radical Biology And Medicine 2017, 112: 22. DOI: 10.1016/j.freeradbiomed.2017.10.019.
- Mycobacterium tuberculosis universal stress protein Rv2623 interacts with the putative ATP binding cassette (ABC) transporter Rv1747 to regulate mycobacterial growthGlass LN, Swapna G, Chavadi SS, Tufariello JM, Mi K, Drumm JE, Lam TT, Zhu G, Zhan C, Vilchéze C, Arcos J, Chen Y, Bi L, Mehta S, Porcelli SA, Almo SC, Yeh SR, Jacobs WR, Torrelles JB, Chan J. Mycobacterium tuberculosis universal stress protein Rv2623 interacts with the putative ATP binding cassette (ABC) transporter Rv1747 to regulate mycobacterial growth PLOS Pathogens 2017, 13: e1006515. PMID: 28753640, PMCID: PMC5549992, DOI: 10.1371/journal.ppat.1006515.
- Loss of TMEM106B Ameliorates Lysosomal and Frontotemporal Dementia-Related Phenotypes in Progranulin-Deficient MiceKlein ZA, Takahashi H, Ma M, Stagi M, Zhou M, Lam TT, Strittmatter SM. Loss of TMEM106B Ameliorates Lysosomal and Frontotemporal Dementia-Related Phenotypes in Progranulin-Deficient Mice Neuron 2017, 95: 281-296.e6. PMID: 28728022, PMCID: PMC5558861, DOI: 10.1016/j.neuron.2017.06.026.
- A Branched-Chain Amino Acid-Related Metabolic Signature Characterizes Obese Adolescents with Non-Alcoholic Fatty Liver DiseaseGoffredo M, Santoro N, Tricò D, Giannini C, D’Adamo E, Zhao H, Peng G, Yu X, Lam TT, Pierpont B, Caprio S, Herzog RI. A Branched-Chain Amino Acid-Related Metabolic Signature Characterizes Obese Adolescents with Non-Alcoholic Fatty Liver Disease Nutrients 2017, 9: 642. PMID: 28640216, PMCID: PMC5537762, DOI: 10.3390/nu9070642.
- Reciprocal regulation of ARPP-16 by PKA and MAST3 kinases provides a cAMP-regulated switch in protein phosphatase 2A inhibitionMusante V, Li L, Kanyo J, Lam TT, Colangelo CM, Cheng SK, Brody AH, Greengard P, Le Novère N, Nairn AC. Reciprocal regulation of ARPP-16 by PKA and MAST3 kinases provides a cAMP-regulated switch in protein phosphatase 2A inhibition ELife 2017, 6: e24998. PMID: 28613156, PMCID: PMC5515580, DOI: 10.7554/elife.24998.
- Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extractSrivastava R, Mannam P, Rauniyar N, Lam TT, Luo R, Lee PJ, Srivastava A. Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extract Data In Brief 2017, 13: 320-325. PMID: 28653025, PMCID: PMC5476452, DOI: 10.1016/j.dib.2017.05.049.
- Brain Region and Isoform-Specific Phosphorylation Alters Kalirin SH2 Domain Interaction Sites and Calpain SensitivityMiller MB, Yan Y, Machida K, Kiraly DD, Levy AD, Wu YI, Lam TT, Abbott T, Koleske AJ, Eipper BA, Mains RE. Brain Region and Isoform-Specific Phosphorylation Alters Kalirin SH2 Domain Interaction Sites and Calpain Sensitivity ACS Chemical Neuroscience 2017, 8: 1554-1569. PMID: 28418645, PMCID: PMC5517348, DOI: 10.1021/acschemneuro.7b00076.
- Rice calcium‐dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose‐phosphate synthase and is required for a proper cold stress responseAlmadanim MC, Alexandre BM, Rosa MTG, Sapeta H, Leitão AE, Ramalho JC, Lam TT, Negrão S, Abreu IA, Oliveira MM. Rice calcium‐dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose‐phosphate synthase and is required for a proper cold stress response Plant Cell & Environment 2017, 40: 1197-1213. PMID: 28102545, DOI: 10.1111/pce.12916.
- Data-Independent Acquisition and Parallel Reaction Monitoring Mass Spectrometry Identification of Serum Biomarkers for Ovarian CancerRauniyar N, Peng G, Lam TT, Zhao H, Mor G, Williams KR. Data-Independent Acquisition and Parallel Reaction Monitoring Mass Spectrometry Identification of Serum Biomarkers for Ovarian Cancer Biomarker Insights 2017, 12: 1177271917710948. PMID: 28615921, PMCID: PMC5462478, DOI: 10.1177/1177271917710948.
- MKK3 influences mitophagy and is involved in cigarette smoke-induced inflammationMannam P, Rauniyar N, Lam TT, Luo R, Lee PJ, Srivastava A. MKK3 influences mitophagy and is involved in cigarette smoke-induced inflammation Free Radical Biology And Medicine 2016, 101: 102-115. PMID: 27717867, DOI: 10.1016/j.freeradbiomed.2016.10.001.
- Phosphoproteomic Analysis Reveals a Novel Mechanism of CaMKIIα Regulation Inversely Induced by Cocaine Memory Extinction versus ReconsolidationRich MT, Abbott TB, Chung L, Gulcicek EE, Stone KL, Colangelo CM, Lam TT, Nairn AC, Taylor JR, Torregrossa MM. Phosphoproteomic Analysis Reveals a Novel Mechanism of CaMKIIα Regulation Inversely Induced by Cocaine Memory Extinction versus Reconsolidation Journal Of Neuroscience 2016, 36: 7613-7627. PMID: 27445140, PMCID: PMC4951572, DOI: 10.1523/jneurosci.1108-16.2016.
- Evaluation of the Nicotinic Acetylcholine Receptor-Associated Proteome at Baseline and Following Nicotine Exposure in Human and Mouse CortexMcClure-Begley TD, Esterlis I, Stone KL, Lam TT, Grady SR, Colangelo CM, Lindstrom JM, Marks MJ, Picciotto MR. Evaluation of the Nicotinic Acetylcholine Receptor-Associated Proteome at Baseline and Following Nicotine Exposure in Human and Mouse Cortex ENeuro 2016, 3: eneuro.0166-16.2016. PMID: 27559543, PMCID: PMC4985585, DOI: 10.1523/eneuro.0166-16.2016.
- O-Glycosylation of a Secretory Granule Membrane Enzyme Is Essential for Its Endocytic Trafficking*Vishwanatha KS, Bäck N, Lam TT, Mains RE, Eipper BA. O-Glycosylation of a Secretory Granule Membrane Enzyme Is Essential for Its Endocytic Trafficking* Journal Of Biological Chemistry 2016, 291: 9835-9850. PMID: 26961877, PMCID: PMC4850319, DOI: 10.1074/jbc.m115.711838.
- SILAC based protein profiling data of MKK3 knockout mouse embryonic fibroblastsSrivastava A, Shinn AS, Lam TT, Lee PJ, Mannam P. SILAC based protein profiling data of MKK3 knockout mouse embryonic fibroblasts Data In Brief 2016, 7: 418-422. PMID: 26977448, PMCID: PMC4782019, DOI: 10.1016/j.dib.2016.02.034.
- Netrin‐1 Regulates Fibrocyte Accumulation in the Decellularized Fibrotic Sclerodermatous Lung Microenvironment and in Bleomycin‐Induced Pulmonary FibrosisSun H, Zhu Y, Pan H, Chen X, Balestrini JL, Lam TT, Kanyo JE, Eichmann A, Gulati M, Fares WH, Bai H, Feghali-Bostwick CA, Gan Y, Peng X, Moore MW, White ES, Sava P, Gonzalez AL, Cheng Y, Niklason LE, Herzog EL. Netrin‐1 Regulates Fibrocyte Accumulation in the Decellularized Fibrotic Sclerodermatous Lung Microenvironment and in Bleomycin‐Induced Pulmonary Fibrosis Arthritis & Rheumatology 2016, 68: 1251-1261. PMID: 26749424, PMCID: PMC5547894, DOI: 10.1002/art.39575.
- Neuronal ceroid lipofuscinosis with DNAJC5/CSPα mutation has PPT1 pathology and exhibit aberrant protein palmitoylationHenderson MX, Wirak GS, Zhang YQ, Dai F, Ginsberg SD, Dolzhanskaya N, Staropoli JF, Nijssen PC, Lam TT, Roth AF, Davis NG, Dawson G, Velinov M, Chandra SS. Neuronal ceroid lipofuscinosis with DNAJC5/CSPα mutation has PPT1 pathology and exhibit aberrant protein palmitoylation Acta Neuropathologica 2015, 131: 621-637. PMID: 26659577, PMCID: PMC4791186, DOI: 10.1007/s00401-015-1512-2.
- MKK3 deletion improves mitochondrial qualitySrivastava A, McGinniss J, Wong Y, Shinn AS, Lam TT, Lee PJ, Mannam P. MKK3 deletion improves mitochondrial quality Free Radical Biology And Medicine 2015, 87: 373-384. PMID: 26119780, DOI: 10.1016/j.freeradbiomed.2015.06.024.
- YPED: An Integrated Bioinformatics Suite and Database for Mass Spectrometry-based Proteomics ResearchColangelo CM, Shifman M, Cheung KH, Stone KL, Carriero NJ, Gulcicek EE, Lam TT, Wu T, Bjornson RD, Bruce C, Nairn AC, Rinehart J, Miller PL, Williams KR. YPED: An Integrated Bioinformatics Suite and Database for Mass Spectrometry-based Proteomics Research Genomics Proteomics & Bioinformatics 2015, 13: 25-35. PMID: 25712262, PMCID: PMC4411476, DOI: 10.1016/j.gpb.2014.11.002.
- Angiotensin II signaling via protein kinase C phosphorylates Kelch-like 3, preventing WNK4 degradationShibata S, Arroyo JP, Castañeda-Bueno M, Puthumana J, Zhang J, Uchida S, Stone KL, Lam TT, Lifton RP. Angiotensin II signaling via protein kinase C phosphorylates Kelch-like 3, preventing WNK4 degradation Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 15556-15561. PMID: 25313067, PMCID: PMC4217463, DOI: 10.1073/pnas.1418342111.
- Inhibitor of the Tyrosine Phosphatase STEP Reverses Cognitive Deficits in a Mouse Model of Alzheimer's DiseaseXu J, Chatterjee M, Baguley TD, Brouillette J, Kurup P, Ghosh D, Kanyo J, Zhang Y, Seyb K, Ononenyi C, Foscue E, Anderson GM, Gresack J, Cuny GD, Glicksman MA, Greengard P, Lam TT, Tautz L, Nairn AC, Ellman JA, Lombroso PJ. Inhibitor of the Tyrosine Phosphatase STEP Reverses Cognitive Deficits in a Mouse Model of Alzheimer's Disease PLOS Biology 2014, 12: e1001923. PMID: 25093460, PMCID: PMC4122355, DOI: 10.1371/journal.pbio.1001923.
- Nonenzymatic domains of Kalirin7 contribute to spine morphogenesis through interactions with phosphoinositides and AblMa XM, Miller MB, Vishwanatha KS, Gross MJ, Wang Y, Abbott T, Lam TT, Mains RE, Eipper BA. Nonenzymatic domains of Kalirin7 contribute to spine morphogenesis through interactions with phosphoinositides and Abl Molecular Biology Of The Cell 2014, 25: 1458-1471. PMID: 24600045, PMCID: PMC4004595, DOI: 10.1091/mbc.e13-04-0215.
- A Gut Lipid Messenger Links Excess Dietary Fat to Dopamine DeficiencyTellez LA, Medina S, Han W, Ferreira JG, Licona-Limón P, Ren X, Lam TT, Schwartz GJ, de Araujo IE. A Gut Lipid Messenger Links Excess Dietary Fat to Dopamine Deficiency Science 2013, 341: 800-802. PMID: 23950538, DOI: 10.1126/science.1239275.
- Palmitoylation of Superoxide Dismutase 1 (SOD1) Is Increased for Familial Amyotrophic Lateral Sclerosis-linked SOD1 Mutants*Antinone SE, Ghadge GD, Lam TT, Wang L, Roos RP, Green WN. Palmitoylation of Superoxide Dismutase 1 (SOD1) Is Increased for Familial Amyotrophic Lateral Sclerosis-linked SOD1 Mutants* Journal Of Biological Chemistry 2013, 288: 21606-21617. PMID: 23760509, PMCID: PMC3724620, DOI: 10.1074/jbc.m113.487231.
- Assembly of the SLIP1–SLBP Complex on Histone mRNA Requires Heterodimerization and Sequential Binding of SLBP Followed by SLIP1Bansal N, Zhang M, Bhaskar A, Itotia P, Lee E, Shlyakhtenko LS, Lam TT, Fritz A, Berezney R, Lyubchenko YL, Stafford WF, Thapar R. Assembly of the SLIP1–SLBP Complex on Histone mRNA Requires Heterodimerization and Sequential Binding of SLBP Followed by SLIP1 Biochemistry 2013, 52: 520-536. PMID: 23286197, PMCID: PMC3580866, DOI: 10.1021/bi301074r.
- The Prolyl Isomerase Pin1 Targets Stem-Loop Binding Protein (SLBP) To Dissociate the SLBP-Histone mRNA Complex Linking Histone mRNA Decay with SLBP UbiquitinationKrishnan N, Lam TT, Fritz A, Rempinski D, O'Loughlin K, Minderman H, Berezney R, Marzluff WF, Thapar R. The Prolyl Isomerase Pin1 Targets Stem-Loop Binding Protein (SLBP) To Dissociate the SLBP-Histone mRNA Complex Linking Histone mRNA Decay with SLBP Ubiquitination Molecular And Cellular Biology 2012, 32: 4306-4322. PMID: 22907757, PMCID: PMC3486140, DOI: 10.1128/mcb.00382-12.
- Endothelial Cell Palmitoylproteomic Identifies Novel Lipid-Modified Targets and Potential Substrates for Protein Acyl TransferasesMarin EP, Derakhshan B, Lam TT, Davalos A, Sessa WC. Endothelial Cell Palmitoylproteomic Identifies Novel Lipid-Modified Targets and Potential Substrates for Protein Acyl Transferases Circulation Research 2012, 110: 1336-1344. PMID: 22496122, PMCID: PMC3428238, DOI: 10.1161/circresaha.112.269514.
- Interaction of the Histone mRNA Hairpin with Stem–Loop Binding Protein (SLBP) and Regulation of the SLBP–RNA Complex by Phosphorylation and Proline IsomerizationZhang M, Lam TT, Tonelli M, Marzluff WF, Thapar R. Interaction of the Histone mRNA Hairpin with Stem–Loop Binding Protein (SLBP) and Regulation of the SLBP–RNA Complex by Phosphorylation and Proline Isomerization Biochemistry 2012, 51: 3215-3231. PMID: 22439849, PMCID: PMC3328597, DOI: 10.1021/bi2018255.
- Cyclin-Dependent Kinase 5 Regulates PSD-95 Ubiquitination in NeuronsBianchetta MJ, Lam TT, Jones SN, Morabito MA. Cyclin-Dependent Kinase 5 Regulates PSD-95 Ubiquitination in Neurons Journal Of Neuroscience 2011, 31: 12029-12035. PMID: 21849563, PMCID: PMC3190401, DOI: 10.1523/jneurosci.2388-11.2011.
- Complex interactions in EML cell stimulation by stem cell factor and IL-3Ye ZJ, Gulcicek E, Stone K, Lam T, Schulz V, Weissman SM. Complex interactions in EML cell stimulation by stem cell factor and IL-3 Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 4882-4887. PMID: 21383156, PMCID: PMC3064389, DOI: 10.1073/pnas.1018002108.
- Functional Genomic and Proteomic Analysis Reveals Disruption of Myelin-Related Genes and Translation in a Mouse Model of Early Life NeglectBordner KA, George ED, Carlyle BC, Duque A, Kitchen RR, Lam TT, Colangelo CM, Stone KL, Abbott TB, Mane SM, Nairn AC, Simen AA. Functional Genomic and Proteomic Analysis Reveals Disruption of Myelin-Related Genes and Translation in a Mouse Model of Early Life Neglect Frontiers In Psychiatry 2011, 2: 18. PMID: 21629843, PMCID: PMC3098717, DOI: 10.3389/fpsyt.2011.00018.
- Case Study: The Unanticipated Loss of N2 from Novel DNA Alkylating Agent Laromustine by Collision‐Induced Dissociation: Novel RearrangementsNassar A, Du J, Roberts D, Lin K, Belcourt M, King I, Lam T. Case Study: The Unanticipated Loss of N2 from Novel DNA Alkylating Agent Laromustine by Collision‐Induced Dissociation: Novel Rearrangements 2010, 217-228. DOI: 10.1002/9780470890387.ch5.
- Case Study: Identification of in vitro Metabolite/Decomposition Products of the Novel DNA Alkylating Agent LaromustineNassar A, Du J, Roberts D, Lin K, Belcourt M, King I, Lam T. Case Study: Identification of in vitro Metabolite/Decomposition Products of the Novel DNA Alkylating Agent Laromustine 2010, 229-244. DOI: 10.1002/9780470890387.ch6.
- N-Glycosylation at the SynCAM (Synaptic Cell Adhesion Molecule) Immunoglobulin Interface Modulates Synaptic Adhesion*Fogel AI, Li Y, Giza J, Wang Q, Lam TT, Modis Y, Biederer T. N-Glycosylation at the SynCAM (Synaptic Cell Adhesion Molecule) Immunoglobulin Interface Modulates Synaptic Adhesion* Journal Of Biological Chemistry 2010, 285: 34864-34874. PMID: 20739279, PMCID: PMC2966101, DOI: 10.1074/jbc.m110.120865.
- Identification of Regulatory Phosphorylation Sites in Slack and Slick Sodium Activated Potassium (KNa) ChannelsFleming M, Kronengold J, Yan Y, Lam T, Gulcicek E, Sigworth F, Nairn A, Kaczmarek L. Identification of Regulatory Phosphorylation Sites in Slack and Slick Sodium Activated Potassium (KNa) Channels Biophysical Journal 2010, 98: 316a. DOI: 10.1016/j.bpj.2009.12.1714.
- Palmitoylation of Nicotinic Acetylcholine ReceptorsAlexander JK, Govind AP, Drisdel RC, Blanton MP, Vallejo Y, Lam TT, Green WN. Palmitoylation of Nicotinic Acetylcholine Receptors Journal Of Molecular Neuroscience 2009, 40: 12-20. PMID: 19693711, PMCID: PMC3523180, DOI: 10.1007/s12031-009-9246-z.
- Diversification of a Salmonella Virulence Protein Function by Ubiquitin-Dependent Differential LocalizationPatel JC, Hueffer K, Lam TT, Galán JE. Diversification of a Salmonella Virulence Protein Function by Ubiquitin-Dependent Differential Localization Cell 2009, 137: 283-294. PMID: 19379694, PMCID: PMC2673707, DOI: 10.1016/j.cell.2009.01.056.
- Keck Foundation Biotechnology Resource Laboratory, Yale University.Stone KL, Bjornson RD, Blasko GG, Bruce C, Cofrancesco R, Carriero NJ, Colangelo CM, Crawford JK, Crawford JM, daSilva NC, Deluca JD, Elliott JI, Elliott MM, Flory PJ, Folta-Stogniew EJ, Gulcicek E, Kong Y, Lam TT, Lee JY, Lin A, LoPresti MB, Mane SM, McMurray WJ, Tikhonova IR, Westman S, Williams NA, Wu TL, Hongyu Z, Williams KR. Keck Foundation Biotechnology Resource Laboratory, Yale University. The Yale Journal Of Biology And Medicine 2007, 80: 195-211. PMID: 18449392, PMCID: PMC2347368.
- Posttranslational modifications on protein kinase c isozymes. Effects of epinephrine and phorbol estersRobles-Flores M, Meléndez L, García W, Mendoza-Hernández G, Lam TT, Castañeda-Patlán C, González-Aguilar H. Posttranslational modifications on protein kinase c isozymes. Effects of epinephrine and phorbol esters Biochimica Et Biophysica Acta 2007, 1783: 695-712. PMID: 18295358, DOI: 10.1016/j.bbamcr.2007.07.011.
- Impact of environmental conditions on the marine natural product bryostatin 1Manning TJ, Rhodes E, Land M, Parkman R, Sumner B, Lam TT, Marshall¶ A, Phillips D. Impact of environmental conditions on the marine natural product bryostatin 1 Natural Product Research 2006, 20: 611-628. PMID: 16835096, DOI: 10.1080/14786410500462645.
- Interaction of packaging motor with the polymerase complex of dsRNA bacteriophageLísal J, Kainov DE, Lam TT, Emmett MR, Wei H, Gottlieb P, Marshall AG, Tuma R. Interaction of packaging motor with the polymerase complex of dsRNA bacteriophage Virology 2006, 351: 73-79. PMID: 16643976, DOI: 10.1016/j.virol.2006.03.025.
- Multicomponent Internal Recalibration of an LC−FTICR-MS Analysis Employing a Partially Characterized Complex Peptide Mixture: Systematic and Random ErrorsYanofsky CM, Bell AW, Lesimple S, Morales F, Lam TT, Blakney GT, Marshall AG, Carrillo B, Lekpor K, Boismenu D, Kearney RE. Multicomponent Internal Recalibration of an LC−FTICR-MS Analysis Employing a Partially Characterized Complex Peptide Mixture: Systematic and Random Errors Analytical Chemistry 2005, 77: 7246-7254. PMID: 16285672, DOI: 10.1021/ac050640q.
- Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for the Analysis of Small Ubiquitin-like Modifier (SUMO) Modification: Identification of Lysines in RanBP2 and SUMO Targeted for Modification during the E3 AutoSUMOylation ReactionCooper HJ, Tatham MH, Jaffray E, Heath JK, Lam TT, Marshall AG, Hay RT. Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for the Analysis of Small Ubiquitin-like Modifier (SUMO) Modification: Identification of Lysines in RanBP2 and SUMO Targeted for Modification during the E3 AutoSUMOylation Reaction Analytical Chemistry 2005, 77: 6310-6319. PMID: 16194093, DOI: 10.1021/ac058019d.
- Identifying bryostatins and potential precursors from the bryozoan Bugula neritinaManning TJ, Land M, Rhodes E, Chamberlin L, Rudloe J, Phillips D, Lam TT, Purcell J, Cooper HJ, Emmett MR, Marshall AG. Identifying bryostatins and potential precursors from the bryozoan Bugula neritina Natural Product Research 2005, 19: 467-491. PMID: 15938194, DOI: 10.1080/14786410412331280041.
- Functional visualization of viral molecular motor by hydrogen-deuterium exchange reveals transient statesLísal J, Lam TT, Kainov DE, Emmett MR, Marshall AG, Tuma R. Functional visualization of viral molecular motor by hydrogen-deuterium exchange reveals transient states Nature Structural & Molecular Biology 2005, 12: 460-466. PMID: 15834422, DOI: 10.1038/nsmb927.
- Identification of Sites of Ubiquitination in Proteins: A Fourier Transform Ion Cyclotron Resonance Mass Spectrometry ApproachCooper HJ, Heath JK, Jaffray E, Hay RT, Lam TT, Marshall AG. Identification of Sites of Ubiquitination in Proteins: A Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Approach Analytical Chemistry 2004, 76: 6982-6988. PMID: 15571350, DOI: 10.1021/ac0401063.
- Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchangeLanman J, Lam TT, Emmett MR, Marshall AG, Sakalian M, Prevelige PE. Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange Nature Structural & Molecular Biology 2004, 11: 676-677. PMID: 15208693, DOI: 10.1038/nsmb790.
- Corrigendum to “Identification of Novel Interactions in HIV-1 Capsid Protein Assembly by High-resolution Mass Spectrometry” [J. Mol. Biol. (2003) 325, 759–772]Lanman J, Lam T, Barnes S, Sakalian M, Emmett M, Marshall A, Prevelige P. Corrigendum to “Identification of Novel Interactions in HIV-1 Capsid Protein Assembly by High-resolution Mass Spectrometry” [J. Mol. Biol. (2003) 325, 759–772] Journal Of Molecular Biology 2003, 334: 1133. DOI: 10.1016/j.jmb.2003.10.046.
- Identification of Novel Interactions in HIV-1 Capsid Protein Assembly by High-resolution Mass SpectrometryLanman J, Lam TT, Barnes S, Sakalian M, Emmett MR, Marshall AG, Prevelige PE. Identification of Novel Interactions in HIV-1 Capsid Protein Assembly by High-resolution Mass Spectrometry Journal Of Molecular Biology 2003, 325: 759-772. PMID: 12507478, DOI: 10.1016/s0022-2836(02)01245-7.
- Mapping of protein:protein contact surfaces by hydrogen/deuterium exchange, followed by on-line high-performance liquid chromatography–electrospray ionization fourier-transform ion-cyclotron-resonance mass analysisLam TT, Lanman JK, Emmett MR, Hendrickson CL, Marshall AG, Prevelige PE. Mapping of protein:protein contact surfaces by hydrogen/deuterium exchange, followed by on-line high-performance liquid chromatography–electrospray ionization fourier-transform ion-cyclotron-resonance mass analysis Journal Of Chromatography A 2002, 982: 85-95. PMID: 12489858, DOI: 10.1016/s0021-9673(02)01357-2.