Research & Publications
Specialized keywords: Proteomics; Mass Spectrometry, Protein Chemistry, Analytical Chemistry, Cancer Research, Neuroproteomics
Extensive Research Description
I am the Director of the Keck MS & Proteomics Resource at the Yale School of Medicine and have been with Yale since 2004. My specific aims are to train, assist, collaborate, and make available my expertise in mass spectrometry to as many investigators of other scientific and biomedical discipline as possible so that they can advance their research. By establishing a well-organized and well-trained core group of staffs who are dedicated to the same philosophy and mission, my team provides the high-quality mass spectrometry data and analyses which have resulted in co-authorships and acknowledgements in many high impact scientific publications. My mass spectrometry (MS) footing grew from my graduate career at Florida State University, where I developed a hydrogen/deuterium exchange workflow to robustly observe protein-protein interaction(s) and structural changes in proteins. The technique led to the discovery of unique conformational changes in HIV-1 viral capsid protein (Lanman, et. al., JMB, 325, 759-772, 2003, Cover). I became involved in many collaborative projects during my postdoctoral work at the National High Magnetic Field Lab and began to shape my career in using my analytical chemistry skillsets in high resolution tandem mass spectrometry to work with a multitude of investigators across many basic scientific and applied biomedical disciplines to understand biological functions at the molecular level. My successful multidisciplinary collaborative interaction with these investigators falls in the areas of protein identification, profiling, and quantitation; protein posttranslational modification (PTM); protein-protein interactions; natural products; metabolites; green chemistry; and organic product syntheses among many others. During my residence within the Keck MS and Proteomics Resource, I collaborated many biomedical and clinical investigators in their aims to better understand protein(s) functions in various relevant disease and cancers studies such as illustrated in Fogel et al, 2010, (which was featured on the cover of J. Biol. Chem. 285, 34864); Musante et al, 2017 (Elife 6); Miller et al, 2017 (ACS Chem Neurosci, 8(7), 1554); Sun et al, 2016 (Arthritis Rheumatology, 68(5), 1252); Tellez et al, 2013 (Sciene 341(6147), 800); Klein et al, 2017 (Neruon 95, p281); Goffredo et al, 2017 (Nutrients, 9, p 642); and Henderson et al, 2016 (Acta Neuropathol 131, p621). Currently I am involved in many projects which utilizes high resolution mass spectrometry (Orbitrap family of mass spectrometers) Label Free Quantitative LC MS and LC MS/MS methodology for protein ID/profiling and quantitation in various biological systems (i.e. brain, serum, plasma, liver, endothelial cells, FACS sorted cells, neurons, worms, mosquitos, flies, etc.), for protein ubiquitinylation, palmitoylation, acetylation, and phosphorylation modification, for small molecule quantitation and structural elucidation along with targeted drug compounds assays. The use of these exceedingly more reproducible high resolution mass spectrometry to facilitate protein and protein posttranslational modification ID and quantitative global profiling provides a very robust and biostatistical confident way to monitor protein changes, functions, and interactions within a complex biological system.
Chromatography, High Pressure Liquid; Lipids; Metabolism; Peptide Mapping; Peptides; Proteins; Research Design; Mass Spectrometry; Tissue Extracts; Spectrometry, Mass, Electrospray Ionization; Proteomics; Tandem Mass Spectrometry
Public Health Interests
Aging; Biomarkers; Cancer; Cardiovascular Diseases; Respiratory Disease/Infections