Maudry Laurent-Rolle
MD, PhD, BS
Assistant Professor
Research & Publications
Biography
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Extensive Research Description
I spent the last 16 years studying the molecular mechanisms utilized by pathogenic viruses, specifically flaviviruses, to antagonize host immune responses. During my training in the Post Baccalaureate Research Education Program (PREP) at the Icahn School of Medicine at Mount Sinai I helped developed a reporter assay system that was used to screen dengue virus-encoded proteins’ ability to inhibit the type I interferon response. During my graduate school years, I was awarded a competitive NIH F31 grant based on my doctoral studies focused on understanding how West Nile virus (WNV), dengue virus (DENV) and yellow fever virus (YFV) inhibit the host innate immune response. I characterized the molecular mechanisms by which these viruses use the nonstructural protein 5 (NS5) to inhibit interferon signaling albeit through distinct mechanisms. My thesis project as well as several collaborations resulted in a provisional vaccine patent, a book chapter, and several articles that have been published in high impact journals.
After completing my Internal Medicine residency, I joined the Infectious Diseases Fellowship program here at Yale University. After the first intensive clinical year, I transitioned to basic science research where I focus on characterizing the molecular mechanism(s) by which the antiviral protein, CMPK2 restricts viral replication. The results of this work may lead to the development of new drug targets. In addition to my research, I am also a lecturer in the department of Molecular Biophysics and Biochemistry here at Yale School of Medicine. My clinical experiences and research training in virology as well as biochemical and immunological methods place me in a position to answer the fundamental questions that will contribute to my overall long term goal of vaccine design and development of antivirals.
Coauthors
Selected Publications
- CMPK2 restricts Zika virus replication by inhibiting viral translationPawlak J, Hsu J, Xia H, Han P, Suh H, Grove T, Morrison J, Shi P, Cresswell P, Laurent-Rolle M. CMPK2 restricts Zika virus replication by inhibiting viral translation. PLOS Pathogens 2023, 19: e1011286. PMID: 37075076, PMCID: PMC10150978, DOI: 10.1371/journal.ppat.1011286.
- SARS-CoV-2 accessory proteins ORF7a and ORF3a use distinct mechanisms to down-regulate MHC-I surface expressionArshad N, Laurent-Rolle M, Ahmed W, Hsu J, Mitchell S, Pawlak J, Sengupta D, Biswas K, Cresswell P. SARS-CoV-2 accessory proteins ORF7a and ORF3a use distinct mechanisms to down-regulate MHC-I surface expression. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 120: e2208525120. PMID: 36574644, PMCID: PMC9910621, DOI: 10.1073/pnas.2208525120.
- Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labelingHsu JC, Pawlak JB, Laurent-Rolle M, Cresswell P. Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling. STAR Protocols 2022, 3: 101654. PMID: 36072758, PMCID: PMC9442383, DOI: 10.1016/j.xpro.2022.101654.
- Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replicationHsu JC, Laurent-Rolle M, Pawlak JB, Xia H, Kunte A, Hee JS, Lim J, Harris LD, Wood JM, Evans GB, Shi PY, Grove TL, Almo SC, Cresswell P. Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication. Molecular Cell 2022, 82: 1631-1642.e6. PMID: 35316659, PMCID: PMC9081181, DOI: 10.1016/j.molcel.2022.02.031.
- Efficacy and Safety of COVID-19 Convalescent Plasma in Hospitalized PatientsOrtigoza MB, Yoon H, Goldfeld KS, Troxel AB, Daily JP, Wu Y, Li Y, Wu D, Cobb GF, Baptiste G, O’Keeffe M, Corpuz MO, Ostrosky-Zeichner L, Amin A, Zacharioudakis IM, Jayaweera DT, Wu Y, Philley JV, Devine MS, Desruisseaux MS, Santin AD, Anjan S, Mathew R, Patel B, Nigo M, Upadhyay R, Kupferman T, Dentino AN, Nanchal R, Merlo CA, Hager DN, Chandran K, Lai JR, Rivera J, Bikash CR, Lasso G, Hilbert TP, Paroder M, Asencio AA, Liu M, Petkova E, Bragat A, Shaker R, McPherson DD, Sacco RL, Keller MJ, Grudzen CR, Hochman JS, Pirofski LA, Rahman F, Ajayi A, Rodriguez S, Ledesma A, Keeling D, Rappoport N, Ebel S, Kim J, Chang M, Chan K, Patel P, Martocci A, Dave S, Darwish Y, Taveras M, Shoyelu V, Xin P, Iturrate E, Moldolsky L, Raimondo B, Mendez S, Hughes P, Sterling S, Lord A, Yaghi S, Veloso K, Sheikh M, Visconti-Ferrara E, Fleming A, Youn H, Jane Fran B, Medina R, McKell R, Khan S, Hamilton T, Sanchez C, Patel N, Cleare L, Vergnolle O, Nakouzi A, Quevedo G, Bortz R, Wirchnianski A, Florez C, Babb R, Ayala J, Tsagaris K, James A, Eke I, Obeidallah A, Sandu O, Sohval S, Serrano-Rahman L, Uehlinger J, Bartash R, Al-Abduladheem A, Gendlina I, Sheridan C, Bortnick A, Eichler J, Kaufman R, Yukelis S, Pennock M, Goggin M, Shen C, Annam J, Khokhar A, Barboto D, Lally B, Lee A, Lee M, Yang X, Allen S, Malaviya A, Moussa O, Park R, Sample R, Bae A, Benoni G, Boerger L, Baker L, Luther M, Ameti L, Briggs N, Golden M, Gormally M, Huang G, Johnson R, Morrison A, Montagna-Hill M, Rivera B, Cortezzo G, Debski K, Nicoletti, DeBenedictis K, Davis R, Marshall C, Duque Cuartas M, Beauchamps L, Bertran-Lopez J, Gonzales Zamora J, Delgado-Lelievre M, Dominguez S, Lee C, Kusack H, Karakeshishyan V, Hajaz A, Deniz D, Garcia G, Dae K, Blenet P, Jaffe D, Olson L, Sabogal D, Blust O, Del Prete Perez V, Bornia C, Rodriguez-Perez V, Calderon V, Ramdev R, Jolly A, Guzman I, Guerra R, Brito S, Hobbs R, Denham R, Dick J, Hernandez M, Nielsen L, Anjum S, Mader S, Stutz T, Mammadova M, Nichols P, Khan T, Boktour M, Castaneda B, Benitez B, Hinojosa E, Guerra B, Ortiz A, Hebbeler-Clark R, McShane P, Hibbard R, Hawkins B, Dohanich E, Wadle C, Greenlee K, Brooks J, Herrick C, Gode A, Bergl P, Hu K, Patel J, Srinivasan S, Graf J, Klis C, Reimer K, Carpenter E, Naczek C, Petersen R, Dex R, Drossart J, Zelten J, Brummitt C, Liang M, Yanny L, Dennison G, Runningen P, Brzezinski B, Fiebig S, Naczek C, Kasdorf M, Parameswaran L, Corcoran A, Rohatgi A, Wronska M, Wu X, Srinivasan R, Deng F, Filardo T, Pendse J, Blaser S, Whyte O, Gallagher J, Thomas O, Ramos D, Sturm-Reganato C, Fong C, Daus I, Payoen A, Chiofolo J, Friedman M, Wu D, Jacobson J, Schneider J, Sarwar U, Wang H, Huebinger R, Dronavalli G, Bai Y, Grimes C, Eldin K, Umana V, Martin J, Heath T, Bello F, Ransford D, Laurent-Rolle M, Shenoi S, Akide-Ndunge O, Thapa B, Peterson J, Knauf K, Patel S, Cheney L, Tormey C, Hendrickson J. Efficacy and Safety of COVID-19 Convalescent Plasma in Hospitalized Patients. JAMA Internal Medicine 2022, 182: 115-126. PMID: 34901997, PMCID: PMC8669605, DOI: 10.1001/jamainternmed.2021.6850.
- Introduction to the special issue on interferon responses: From cells to systemsMorrison J, Laurent-Rolle M. Introduction to the special issue on interferon responses: From cells to systems. Current Research In Virological Science 2022, 3: 100020. PMID: 35072125, PMCID: PMC8767929, DOI: 10.1016/j.crviro.2022.100020.
- Early but not late convalescent plasma is associated with better survival in moderate-to-severe COVID-19Briggs N, Gormally MV, Li F, Browning SL, Treggiari MM, Morrison A, Laurent-Rolle M, Deng Y, Hendrickson JE, Tormey CA, Desruisseaux MS. Early but not late convalescent plasma is associated with better survival in moderate-to-severe COVID-19. PLOS ONE 2021, 16: e0254453. PMID: 34320004, PMCID: PMC8318280, DOI: 10.1371/journal.pone.0254453.
- Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 proteinHsu JC, Laurent-Rolle M, Pawlak JB, Wilen CB, Cresswell P. Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2101161118. PMID: 34045361, PMCID: PMC8214666, DOI: 10.1073/pnas.2101161118.
- SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2Lu Q, Liu J, Zhao S, Gomez Castro MF, Laurent-Rolle M, Dong J, Ran X, Damani-Yokota P, Tang H, Karakousi T, Son J, Kaczmarek ME, Zhang Z, Yeung ST, McCune BT, Chen RE, Tang F, Ren X, Chen X, Hsu JCC, Teplova M, Huang B, Deng H, Long Z, Mudianto T, Jin S, Lin P, Du J, Zang R, Su TT, Herrera A, Zhou M, Yan R, Cui J, Zhu J, Zhou Q, Wang T, Ma J, Koralov SB, Zhang Z, Aifantis I, Segal LN, Diamond MS, Khanna KM, Stapleford KA, Cresswell P, Liu Y, Ding S, Xie Q, Wang J. SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2. Immunity 2021, 54: 1304-1319.e9. PMID: 34048708, PMCID: PMC8106883, DOI: 10.1016/j.immuni.2021.05.006.
- Translational regulation of viral RNA in the type I interferon responseHsu J, Laurent-Rolle M, Cresswell P. Translational regulation of viral RNA in the type I interferon response. Current Research In Virological Science 2021, 2: 100012. DOI: 10.1016/j.crviro.2021.100012.
- Use of Convalescent Plasma Therapy in Severe Coronavirus Disease 2019: The Yale-New Haven Health System ExperienceBrowning S, Gormally M, Briggs N, Li M, Zakko A, Laurent-Rolle M, Ladines-Lim J, Morrison A, Kandel P, Khan M, McLeod G, Buller G, Owusu K, Treggiari M, Hendrickson J, Tormey C, Desruisseaux M. Use of Convalescent Plasma Therapy in Severe Coronavirus Disease 2019: The Yale-New Haven Health System Experience. Blood 2020, 136: 39-40. PMCID: PMC8330251, DOI: 10.1182/blood-2020-137352.
- Prolonged incubation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a patient on rituximab therapyKoff A, Laurent-Rolle M, Hsu JC, Malinis M. Prolonged incubation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a patient on rituximab therapy. Infection Control And Hospital Epidemiology 2020, 42: 1286-1288. PMID: 33023685, PMCID: PMC7578652, DOI: 10.1017/ice.2020.1239.
- The Role of NS5 Protein in Determination of Host Cell Range for Yellow Fever VirusLaurent-Rolle M, Morrison J. The Role of NS5 Protein in Determination of Host Cell Range for Yellow Fever Virus. DNA And Cell Biology 2019, 38: 1414-1417. PMID: 31633391, DOI: 10.1089/dna.2019.5115.
- Host-Specific NS5 Ubiquitination Determines Yellow Fever Virus Tropism.Miorin L, Laurent-Rolle M, Pisanelli G, Co PH, Albrecht RA, García-Sastre A, Morrison J. Host-Specific NS5 Ubiquitination Determines Yellow Fever Virus Tropism. Journal Of Virology 2019, 93 PMID: 31043530, PMCID: PMC6600188, DOI: 10.1128/jvi.00151-19.
- STAT2 is a determinant of yellow fever virus host tropismMorrison J, Miorin L, Laurent-Rolle M, Pisanelli G, Co P, Albrecht R, García-Sastre A. STAT2 is a determinant of yellow fever virus host tropism. The Journal Of Immunology 2019, 202: 127.18-127.18. DOI: 10.4049/jimmunol.202.supp.127.18.
- Abstract B163: Regulation of translation by the interferon-induced antiviral protein viperinHsu C, Laurent-Rolle M, Cresswell P. Abstract B163: Regulation of translation by the interferon-induced antiviral protein viperin. Cancer Immunology Research 2019, 7: b163-b163. DOI: 10.1158/2326-6074.cricimteatiaacr18-b163.
- La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocationPisanelli G, Laurent-Rolle M, Manicassamy B, Belicha-Villanueva A, Morrison J, Lozano-Dubernard B, Castro-Peralta F, Iovane G, García-Sastre. A. La Piedad Michoacán Mexico Virus V protein antagonizes type I interferon response by binding STAT2 protein and preventing STATs nuclear translocation. Virus Research 2015, 213: 11-22. PMID: 26546155, PMCID: PMC5538256, DOI: 10.1016/j.virusres.2015.10.027.
- ID: 121 STAT2 is a determinant of yellow fever virus host tropismPisanelli G, Laurent-Rolle M, Morrison J, Garcia-Sastre A. ID: 121 STAT2 is a determinant of yellow fever virus host tropism. Cytokine 2015, 76: 88. DOI: 10.1016/j.cyto.2015.08.148.
- The role of unanchored polyubiquitin chains and the TRIM E3-ubiquitin ligase family of proteins in the innate immune response to influenza virus infection (INM3P.402)Rajsbaum R, Bharaj P, Ellis J, Versteeg G, Schmid S, Maestre A, Belicha-Villanueva A, Patel J, Morrison J, Giuseppe Pisanelli G, Miorin L, Martínez-Romero C, Laurent-Rolle M, Moulton H, Stein D, Fernandez-Sesma A, tenOever B, Garcia-Sastre A. The role of unanchored polyubiquitin chains and the TRIM E3-ubiquitin ligase family of proteins in the innate immune response to influenza virus infection (INM3P.402). The Journal Of Immunology 2015, 194: 127.7-127.7. DOI: 10.4049/jimmunol.194.supp.127.7.
- The Interferon Signaling Antagonist Function of Yellow Fever Virus NS5 Protein Is Activated by Type I InterferonLaurent-Rolle M, Morrison J, Rajsbaum R, Macleod JML, Pisanelli G, Pham A, Ayllon J, Miorin L, Martínez-Romero C, tenOever BR, García-Sastre A. The Interferon Signaling Antagonist Function of Yellow Fever Virus NS5 Protein Is Activated by Type I Interferon. Cell Host & Microbe 2014, 16: 314-327. PMID: 25211074, PMCID: PMC4176702, DOI: 10.1016/j.chom.2014.07.015.
- Unanchored K48-Linked Polyubiquitin Synthesized by the E3-Ubiquitin Ligase TRIM6 Stimulates the Interferon-IKKε Kinase-Mediated Antiviral ResponseRajsbaum R, Versteeg GA, Schmid S, Maestre AM, Belicha-Villanueva A, Martínez-Romero C, Patel JR, Morrison J, Pisanelli G, Miorin L, Laurent-Rolle M, Moulton HM, Stein DA, Fernandez-Sesma A, tenOever BR, García-Sastre A. Unanchored K48-Linked Polyubiquitin Synthesized by the E3-Ubiquitin Ligase TRIM6 Stimulates the Interferon-IKKε Kinase-Mediated Antiviral Response. Immunity 2014, 40: 880-895. PMID: 24882218, PMCID: PMC4114019, DOI: 10.1016/j.immuni.2014.04.018.
- Dengue Virus Co-opts UBR4 to Degrade STAT2 and Antagonize Type I Interferon SignalingMorrison J, Laurent-Rolle M, Maestre AM, Rajsbaum R, Pisanelli G, Simon V, Mulder LC, Fernandez-Sesma A, García-Sastre A. Dengue Virus Co-opts UBR4 to Degrade STAT2 and Antagonize Type I Interferon Signaling. PLOS Pathogens 2013, 9: e1003265. PMID: 23555265, PMCID: PMC3610674, DOI: 10.1371/journal.ppat.1003265.
- Innate Immunity and Flavivirus Infection. Molecular Virology and Control of FlavivirusesCaister Academic Press. Chapter 7
- Mouse STAT2 Restricts Early Dengue Virus ReplicationAshour J, Morrison J, Laurent-Rolle M, Belicha-Villanueva A, Plumlee CR, Bernal-Rubio D, Williams KL, Harris E, Fernandez-Sesma A, Schindler C, García-Sastre A. Mouse STAT2 Restricts Early Dengue Virus Replication. Cell Host & Microbe 2010, 8: 410-421. PMID: 21075352, PMCID: PMC3310429, DOI: 10.1016/j.chom.2010.10.007.
- The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling▿Laurent-Rolle M, Boer EF, Lubick KJ, Wolfinbarger JB, Carmody AB, Rockx B, Liu W, Ashour J, Shupert WL, Holbrook MR, Barrett AD, Mason PW, Bloom ME, García-Sastre A, Khromykh AA, Best SM. The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling▿. Journal Of Virology 2010, 84: 3503-3515. PMID: 20106931, PMCID: PMC2838099, DOI: 10.1128/jvi.01161-09.
- Mouse STAT2 is a dengue virus host restriction factorAshour J, Laurent-Rolle M, Plumlee C, Bernal D, Fernandez-Sesma A, Schindler C, García-Sastre A. Mouse STAT2 is a dengue virus host restriction factor. Cytokine 2009, 48: 62-63. DOI: 10.1016/j.cyto.2009.07.198.
- NS5 of Dengue Virus Mediates STAT2 Binding and Degradation▿Ashour J, Laurent-Rolle M, Shi PY, García-Sastre A. NS5 of Dengue Virus Mediates STAT2 Binding and Degradation▿. Journal Of Virology 2009, 83: 5408-5418. PMID: 19279106, PMCID: PMC2681973, DOI: 10.1128/jvi.02188-08.
- Inhibition of Alpha/Beta Interferon Signaling by the NS4B Protein of FlavivirusesMuñoz-Jordán JL, Laurent-Rolle M, Ashour J, Martínez-Sobrido L, Ashok M, Lipkin WI, García-Sastre A. Inhibition of Alpha/Beta Interferon Signaling by the NS4B Protein of Flaviviruses. Journal Of Virology 2005, 79: 8004-8013. PMID: 15956546, PMCID: PMC1143737, DOI: 10.1128/jvi.79.13.8004-8013.2005.
- Inhibition of interferon signaling by dengue virusMuñoz-Jordán J, Sánchez-Burgos GG, Laurent-Rolle M, García-Sastre A. Inhibition of interferon signaling by dengue virus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 14333-14338. PMID: 14612562, PMCID: PMC283592, DOI: 10.1073/pnas.2335168100.