Stacy Malaker
Research & Publications
Biography
News
Research Summary
Over the last few decades, mass spectrometry (MS) has revolutionized the study of proteins, and workflows for unmodified proteins have become routine. Generally, proteins are digested with trypsin, subjected to positive mode electrospray ionization, fragmented by well-documented methods, and the resultant spectra can be searched using a wide variety of reliable search algorithms.
However, the O-glycoproteome is wholly uncharted territory, as densely O-glycosylated domains (aka mucin domains) do not fit into the workflows developed for traditional proteomics. My laboratory is developing original methods to tackle these problems, thus shedding light on the mucinome. Additionally, we use these techniques to investigate the role of mucins in the context of inflammatory bowel disease, cancer, cystic fibrosis, and autoimmune disorders.
Coauthors
Selected Publications
- Glycoproteomics: Charting new territory in mass spectrometry and glycobiologyMalaker S. Glycoproteomics: Charting new territory in mass spectrometry and glycobiology. Journal Of Mass Spectrometry 2024, 59: e5034. PMID: 38726698, DOI: 10.1002/jms.5034.
- Stress-induced mucin 13 reductions drive intestinal microbiome shifts and despair behaviorsRivet-Noor C, Merchak A, Render C, Gay N, Beiter R, Brown R, Keeler A, Moreau G, Li S, Olgun D, Steigmeyer A, Ofer R, Phan T, Vemuri K, Chen L, Mahoney K, Shin J, Malaker S, Deppmann C, Verzi M, Gaultier A. Stress-induced mucin 13 reductions drive intestinal microbiome shifts and despair behaviors. Brain Behavior And Immunity 2024, 119: 665-680. PMID: 38579936, DOI: 10.1016/j.bbi.2024.03.028.
- Mass Spectrometry-Compatible Elution Technique Enables an Improved Mucin-Selective Enrichment Strategy to Probe the MucinomeMahoney K, Chang V, Lucas T, Maruszko K, Malaker S. Mass Spectrometry-Compatible Elution Technique Enables an Improved Mucin-Selective Enrichment Strategy to Probe the Mucinome. Analytical Chemistry 2024, 96: 5242-5250. PMID: 38512228, DOI: 10.1021/acs.analchem.3c05762.
- Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SME and molecular dynamics simulationsRosenfeld M, Kearns F, Chongsaritsinsuk J, Steigmeyer A, Mahoney K, Lucas T, Ince D, Battison A, Hollenhorst M, Shon D, Bertozzi C, Farracane M, Lemmon M, Malaker S, Amaro R. Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SME and molecular dynamics simulations. Biophysical Journal 2024, 123: 14a. DOI: 10.1016/j.bpj.2023.11.205.
- Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmEChongsaritsinsuk J, Steigmeyer A, Mahoney K, Rosenfeld M, Lucas T, Smith C, Li A, Ince D, Kearns F, Battison A, Hollenhorst M, Judy Shon D, Tiemeyer K, Attah V, Kwon C, Bertozzi C, Ferracane M, Lemmon M, Amaro R, Malaker S. Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE. Nature Communications 2023, 14: 6169. PMID: 37794035, PMCID: PMC10550946, DOI: 10.1038/s41467-023-41756-y.
- False-Positive Glycopeptide Identification via In-FAIMS FragmentationRangel-Angarita V, Mahoney K, Kwon C, Sarker R, Lucas T, Malaker S. False-Positive Glycopeptide Identification via In-FAIMS Fragmentation. JACS Au 2023, 3: 2498-2509. PMID: 37772174, PMCID: PMC10523363, DOI: 10.1021/jacsau.3c00264.
- Design of a mucin-selective protease for targeted degradation of cancer-associated mucinsPedram K, Shon D, Tender G, Mantuano N, Northey J, Metcalf K, Wisnovsky S, Riley N, Forcina G, Malaker S, Kuo A, George B, Miller C, Casey K, Vilches-Moure J, Ferracane M, Weaver V, Läubli H, Bertozzi C. Design of a mucin-selective protease for targeted degradation of cancer-associated mucins. Nature Biotechnology 2023, 42: 597-607. PMID: 37537499, PMCID: PMC11018308, DOI: 10.1038/s41587-023-01840-6.
- Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activityHollander M, Malaker S, Riley N, Perez I, Abney N, Gray M, Maxson J, Cochran J, Bertozzi C. Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activity. Journal Of Biological Chemistry 2023, 299: 104755. PMID: 37116708, PMCID: PMC10245049, DOI: 10.1016/j.jbc.2023.104755.
- Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonizationHuang X, Nero T, Weerasekera R, Matej K, Hinbest A, Jiang Z, Lee R, Wu L, Chak C, Nijjer J, Gibaldi I, Yang H, Gamble N, Ng W, Malaker S, Sumigray K, Olson R, Yan J. Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization. Nature Communications 2023, 14: 2104. PMID: 37055389, PMCID: PMC10102183, DOI: 10.1038/s41467-023-37660-0.
- The 2022 Nobel Prize in Chemistry-sweet!Boyce M, Malaker S, Riley N, Kohler J. The 2022 Nobel Prize in Chemistry-sweet! Glycobiology 2023, 33: 178-181. PMID: 36892406, DOI: 10.1093/glycob/cwad016.
- O‑Linked Sialoglycans Modulate the Proteolysis of SARS-CoV‑2 Spike and Likely Contribute to the Mutational Trajectory in Variants of ConcernGonzalez-Rodriguez E, Zol-Hanlon M, Bineva-Todd G, Marchesi A, Skehel M, Mahoney K, Roustan C, Borg A, Di Vagno L, Kjær S, Wrobel A, Benton D, Nawrath P, Flitsch S, Joshi D, González-Ramírez A, Wilkinson K, Wilkinson R, Wall E, Hurtado-Guerrero R, Malaker S, Schumann B. O‑Linked Sialoglycans Modulate the Proteolysis of SARS-CoV‑2 Spike and Likely Contribute to the Mutational Trajectory in Variants of Concern. ACS Central Science 2023, 9: 393-404. PMID: 36968546, PMCID: PMC10037455, DOI: 10.1021/acscentsci.2c01349.
- T-cell immunoglobulin and mucin-domain containing protein (TIM) structural dynamics revealed by molecular dynamics simulationsRosenfeld M, Kearns F, Chongsaritsinsuk J, Steigmeyer A, Mahoney K, Lucas T, Ince D, Battison A, Hollenhorst M, Shon D, Attah V, Kwon C, Bertozzi C, Malaker S, Amaro R. T-cell immunoglobulin and mucin-domain containing protein (TIM) structural dynamics revealed by molecular dynamics simulations. Biophysical Journal 2023, 122: 204a. DOI: 10.1016/j.bpj.2022.11.1229.
- Comprehensive analysis of platelet glycoprotein Ibα ectodomain glycosylationHollenhorst M, Tiemeyer K, Mahoney K, Aoki K, Ishihara M, Lowery S, Rangel-Angarita V, Bertozzi C, Malaker S. Comprehensive analysis of platelet glycoprotein Ibα ectodomain glycosylation. Journal Of Thrombosis And Haemostasis 2023, 21: 995-1009. PMID: 36740532, PMCID: PMC10065957, DOI: 10.1016/j.jtha.2023.01.009.
- Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cellsCalle B, Gonzalez-Rodriguez E, Mahoney K, Cioce A, Bineva-Todd G, Tastan O, Roustan C, Flynn H, Malaker S, Schumann B. Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells. STAR Protocols 2023, 4: 101974. PMID: 36633947, PMCID: PMC9843269, DOI: 10.1016/j.xpro.2022.101974.
- Cell-specific bioorthogonal tagging of glycoproteinsCioce A, Calle B, Rizou T, Lowery SC, Bridgeman VL, Mahoney KE, Marchesi A, Bineva-Todd G, Flynn H, Li Z, Tastan OY, Roustan C, Soro-Barrio P, Rafiee MR, Garza-Garcia A, Antonopoulos A, Wood TM, Keenan T, Both P, Huang K, Parmeggian F, Snijders AP, Skehel M, Kjær S, Fascione MA, Bertozzi CR, Haslam SM, Flitsch SL, Malaker SA, Malanchi I, Schumann B. Cell-specific bioorthogonal tagging of glycoproteins. Nature Communications 2022, 13: 6237. PMID: 36284108, PMCID: PMC9596482, DOI: 10.1038/s41467-022-33854-0.
- A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bondMedley BJ, Leclaire L, Thompson N, Mahoney KE, Pluvinage B, Parson MAH, Burke JE, Malaker S, Wakarchuk W, Boraston AB. A previously uncharacterized O-glycopeptidase from Akkermansia muciniphila requires the Tn-antigen for cleavage of the peptide bond. Journal Of Biological Chemistry 2022, 298: 102439. PMID: 36049519, PMCID: PMC9513282, DOI: 10.1016/j.jbc.2022.102439.
- GlycoproteomicsBagdonaite I, Malaker S, Polasky D, Riley N, Schjoldager K, Vakhrushev S, Halim A, Aoki-Kinoshita K, Nesvizhskii A, Bertozzi C, Wandall H, Parker B, Thaysen-Andersen M, Scott N. Glycoproteomics. Nature Reviews Methods Primers 2022, 2: 48. DOI: 10.1038/s43586-022-00128-4.
- Current strategies for characterization of mucin-domain glycoproteinsInce D, Lucas TM, Malaker SA. Current strategies for characterization of mucin-domain glycoproteins. Current Opinion In Chemical Biology 2022, 69: 102174. PMID: 35752002, DOI: 10.1016/j.cbpa.2022.102174.
- Revealing the human mucinomeMalaker SA, Riley NM, Shon DJ, Pedram K, Krishnan V, Dorigo O, Bertozzi CR. Revealing the human mucinome. Nature Communications 2022, 13: 3542. PMID: 35725833, PMCID: PMC9209528, DOI: 10.1038/s41467-022-31062-4.
- Mucus sialylation determines intestinal host-commensal homeostasisYao Y, Kim G, Shafer S, Chen Z, Kubo S, Ji Y, Luo J, Yang W, Perner SP, Kanellopoulou C, Park AY, Jiang P, Li J, Baris S, Aydiner EK, Ertem D, Mulder DJ, Warner N, Griffiths AM, Topf-Olivestone C, Kori M, Werner L, Ouahed J, Field M, Liu C, Schwarz B, Bosio CM, Ganesan S, Song J, Urlaub H, Oellerich T, Malaker SA, Zheng L, Bertozzi CR, Zhang Y, Matthews H, Montgomery W, Shih HY, Jiang J, Jones M, Baras A, Shuldiner A, Gonzaga-Jauregui C, Snapper SB, Muise AM, Shouval DS, Ozen A, Pan KT, Wu C, Lenardo MJ. Mucus sialylation determines intestinal host-commensal homeostasis. Cell 2022, 185: 1172-1188.e28. PMID: 35303419, PMCID: PMC9088855, DOI: 10.1016/j.cell.2022.02.013.
- Interleukin-22 regulates B3GNT7 expression to induce fucosylation of glycoproteins in intestinal epithelial cellsCarroll DJ, Burns MWN, Mottram L, Propheter DC, Boucher A, Lessen GM, Kumar A, Malaker SA, Xing C, Hooper LV, Yrlid U, Kohler JJ. Interleukin-22 regulates B3GNT7 expression to induce fucosylation of glycoproteins in intestinal epithelial cells. Journal Of Biological Chemistry 2021, 298: 101463. PMID: 34864058, PMCID: PMC8808068, DOI: 10.1016/j.jbc.2021.101463.
- Novel Antibodies for the Simple and Efficient Enrichment of Native O-GlcNAc Modified PeptidesBurt RA, Dejanovic B, Peckham HJ, Lee KA, Li X, Ounadjela JR, Rao A, Malaker SA, Carr SA, Myers SA. Novel Antibodies for the Simple and Efficient Enrichment of Native O-GlcNAc Modified Peptides. Molecular & Cellular Proteomics 2021, 20: 100167. PMID: 34678516, PMCID: PMC8605273, DOI: 10.1016/j.mcpro.2021.100167.
- Tumor Infiltrating Lymphocytes Target HLA-I Phosphopeptides Derived From Cancer Signaling in Colorectal CancerPenny SA, Abelin JG, Malaker SA, Myers PT, Saeed AZ, Steadman LG, Bai DL, Ward ST, Shabanowitz J, Hunt DF, Cobbold M. Tumor Infiltrating Lymphocytes Target HLA-I Phosphopeptides Derived From Cancer Signaling in Colorectal Cancer. Frontiers In Immunology 2021, 12: 723566. PMID: 34504498, PMCID: PMC8421858, DOI: 10.3389/fimmu.2021.723566.
- Mucinomics as the Next Frontier of Mass SpectrometryRangel-Angarita V, Malaker SA. Mucinomics as the Next Frontier of Mass Spectrometry. ACS Chemical Biology 2021, 16: 1866-1883. PMID: 34319686, DOI: 10.1021/acschembio.1c00384.
- On-tissue spatially resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscapeMalaker SA, Quanico J, Raffo-Romero A, Kobeissy F, Aboulouard S, Tierny D, Bertozzi CR, Fournier I, Salzet M. On-tissue spatially resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape. Cell Chemical Biology 2021, 29: 30-42.e4. PMID: 34102146, PMCID: PMC8617081, DOI: 10.1016/j.chembiol.2021.05.007.
- Small RNAs are modified with N-glycans and displayed on the surface of living cellsFlynn RA, Pedram K, Malaker SA, Batista PJ, Smith BAH, Johnson AG, George BM, Majzoub K, Villalta PW, Carette JE, Bertozzi CR. Small RNAs are modified with N-glycans and displayed on the surface of living cells. Cell 2021, 184: 3109-3124.e22. PMID: 34004145, PMCID: PMC9097497, DOI: 10.1016/j.cell.2021.04.023.
- Novel “mucinomics” platform reveals molecular signatures of cancer in cellular systems and ovarian cancer patient ascites fluidMalaker S, Riley N, Shon J, Pedram K, Bertozzi C. Novel “mucinomics” platform reveals molecular signatures of cancer in cellular systems and ovarian cancer patient ascites fluid. The FASEB Journal 2021, 35 DOI: 10.1096/fasebj.2021.35.s1.00426.
- Classification, structural biology, and applications of mucin domain-targeting proteasesShon DJ, Kuo A, Ferracane MJ, Malaker SA. Classification, structural biology, and applications of mucin domain-targeting proteases. Biochemical Journal 2021, 478: 1585-1603. PMID: 33909028, DOI: 10.1042/bcj20200607.
- Benefits of Chemical Sugar Modifications Introduced by Click Chemistry for Glycoproteomic AnalysesCalle B, Bineva-Todd G, Marchesi A, Flynn H, Ghirardello M, Tastan OY, Roustan C, Choi J, Galan MC, Schumann B, Malaker SA. Benefits of Chemical Sugar Modifications Introduced by Click Chemistry for Glycoproteomic Analyses. Journal Of The American Society For Mass Spectrometry 2021, 32: 2366-2375. PMID: 33871988, PMCID: PMC7611619, DOI: 10.1021/jasms.1c00084.
- VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector ImpuritiesRumachik NG, Malaker SA, Paulk NK. VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector Impurities. Frontiers In Immunology 2021, 12: 657795. PMID: 33868302, PMCID: PMC8047074, DOI: 10.3389/fimmu.2021.657795.
- Genome-wide CRISPR screens reveal a specific ligand for the glycan-binding immune checkpoint receptor Siglec-7Wisnovsky S, Möckl L, Malaker SA, Pedram K, Hess GT, Riley NM, Gray MA, Smith BAH, Bassik MC, Moerner WE, Bertozzi CR. Genome-wide CRISPR screens reveal a specific ligand for the glycan-binding immune checkpoint receptor Siglec-7. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2015024118. PMID: 33495350, PMCID: PMC7865165, DOI: 10.1073/pnas.2015024118.
- Electron-Based Dissociation Is Needed for O‑Glycopeptides Derived from OpeRATOR ProteolysisRiley NM, Malaker SA, Bertozzi CR. Electron-Based Dissociation Is Needed for O‑Glycopeptides Derived from OpeRATOR Proteolysis. Analytical Chemistry 2020, 92: 14878-14884. PMID: 33125225, PMCID: PMC8329938, DOI: 10.1021/acs.analchem.0c02950.
- Generating orthogonal glycosyltransferase and nucleotide sugar pairs as next-generation glycobiology toolsCioce A, Malaker SA, Schumann B. Generating orthogonal glycosyltransferase and nucleotide sugar pairs as next-generation glycobiology tools. Current Opinion In Chemical Biology 2020, 60: 66-78. PMID: 33125942, PMCID: PMC7955280, DOI: 10.1016/j.cbpa.2020.09.001.
- Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylationDebets MF, Tastan OY, Wisnovsky SP, Malaker SA, Angelis N, Moeckl LKR, Choi J, Flynn H, Wagner LJS, Bineva-Todd G, Antonopoulos A, Cioce A, Browne WM, Li Z, Briggs DC, Douglas HL, Hess GT, Agbay AJ, Roustan C, Kjaer S, Haslam SM, Snijders AP, Bassik MC, Moerner WE, Li VSW, Bertozzi CR, Schumann B. Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 25293-25301. PMID: 32989128, PMCID: PMC7568240, DOI: 10.1073/pnas.2007297117.
- An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteinsShon DJ, Malaker SA, Pedram K, Yang E, Krishnan V, Dorigo O, Bertozzi CR. An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteins. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 21299-21307. PMID: 32817557, PMCID: PMC7474620, DOI: 10.1073/pnas.2012196117.
- Targeted glycan degradation potentiates the anticancer immune response in vivoGray MA, Stanczak MA, Mantuano NR, Xiao H, Pijnenborg JFA, Malaker SA, Miller CL, Weidenbacher PA, Tanzo JT, Ahn G, Woods EC, Läubli H, Bertozzi CR. Targeted glycan degradation potentiates the anticancer immune response in vivo. Nature Chemical Biology 2020, 16: 1376-1384. PMID: 32807964, PMCID: PMC7727925, DOI: 10.1038/s41589-020-0622-x.
- Optimal Dissociation Methods Differ for N- and O‑GlycopeptidesRiley NM, Malaker SA, Driessen MD, Bertozzi CR. Optimal Dissociation Methods Differ for N- and O‑Glycopeptides. Journal Of Proteome Research 2020, 19: 3286-3301. PMID: 32500713, PMCID: PMC7425838, DOI: 10.1021/acs.jproteome.0c00218.
- Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct VectorsRumachik NG, Malaker SA, Poweleit N, Maynard LH, Adams CM, Leib RD, Cirolia G, Thomas D, Stamnes S, Holt K, Sinn P, May AP, Paulk NK. Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors. Molecular Therapy — Methods & Clinical Development 2020, 18: 98-118. PMID: 32995354, PMCID: PMC7488757, DOI: 10.1016/j.omtm.2020.05.018.
- Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living CellsSchumann B, Malaker SA, Wisnovsky SP, Debets MF, Agbay AJ, Fernandez D, Wagner LJS, Lin L, Li Z, Choi J, Fox DM, Peh J, Gray MA, Pedram K, Kohler JJ, Mrksich M, Bertozzi CR. Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells. Molecular Cell 2020, 78: 824-834.e15. PMID: 32325029, PMCID: PMC7276986, DOI: 10.1016/j.molcel.2020.03.030.
- Engineering Orthogonal Polypeptide GalNAc-Transferase and UDP-Sugar PairsChoi J, Wagner LJS, Timmermans SBPE, Malaker SA, Schumann B, Gray MA, Debets MF, Takashima M, Gehring J, Bertozzi CR. Engineering Orthogonal Polypeptide GalNAc-Transferase and UDP-Sugar Pairs. Journal Of The American Chemical Society 2019, 141: 13442-13453. PMID: 31373799, PMCID: PMC6813768, DOI: 10.1021/jacs.9b04695.
- Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity PurificationPenny SA, Malaker SA. Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification. 2019, 2024: 235-243. PMID: 31364053, DOI: 10.1007/978-1-4939-9597-4_14.
- Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing PathwaysMalaker SA, Ferracane MJ. Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways. 2019, 2024: 269-285. PMID: 31364056, DOI: 10.1007/978-1-4939-9597-4_17.
- The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucinsMalaker SA, Pedram K, Ferracane MJ, Bensing BA, Krishnan V, Pett C, Yu J, Woods EC, Kramer JR, Westerlind U, Dorigo O, Bertozzi CR. The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 7278-7287. PMID: 30910957, PMCID: PMC6462054, DOI: 10.1073/pnas.1813020116.
- Shared peptide binding of HLA Class I and II alleles associate with cutaneous nevirapine hypersensitivity and identify novel risk allelesPavlos R, McKinnon EJ, Ostrov DA, Peters B, Buus S, Koelle D, Chopra A, Schutte R, Rive C, Redwood A, Restrepo S, Bracey A, Kaever T, Myers P, Speers E, Malaker SA, Shabanowitz J, Jing Y, Gaudieri S, Hunt DF, Carrington M, Haas DW, Mallal S, Phillips EJ. Shared peptide binding of HLA Class I and II alleles associate with cutaneous nevirapine hypersensitivity and identify novel risk alleles. Scientific Reports 2017, 7: 8653. PMID: 28819312, PMCID: PMC5561238, DOI: 10.1038/s41598-017-08876-0.
- Canonical and cross-reactive binding of NK cell inhibitory receptors to HLA-C allotypes is dictated by peptides bound to HLA-CSim M, Malaker S, Khan A, Stowell J, Shabanowitz J, Peterson M, Rajagopalan S, Hunt D, Altmann D, Long E, Boyton R. Canonical and cross-reactive binding of NK cell inhibitory receptors to HLA-C allotypes is dictated by peptides bound to HLA-C. The Journal Of Immunology 2017, 198: 222.25-222.25. DOI: 10.4049/jimmunol.198.supp.222.25.
- Identification of Glycopeptides as Posttranslationally Modified Neoantigens in LeukemiaMalaker SA, Penny SA, Steadman LG, Myers PT, Loke JC, Raghavan M, Bai DL, Shabanowitz J, Hunt DF, Cobbold M. Identification of Glycopeptides as Posttranslationally Modified Neoantigens in Leukemia. Cancer Immunology Research 2017, 5: 376-384. PMID: 28314751, PMCID: PMC5508727, DOI: 10.1158/2326-6066.cir-16-0280.
- Peptide-binding motifs of two common equine class I MHC molecules in Thoroughbred horsesBergmann T, Lindvall M, Moore E, Moore E, Sidney J, Miller D, Tallmadge RL, Myers PT, Malaker SA, Shabanowitz J, Osterrieder N, Peters B, Hunt DF, Antczak DF, Sette A. Peptide-binding motifs of two common equine class I MHC molecules in Thoroughbred horses. Immunogenetics 2017, 69: 351-358. PMID: 28315936, PMCID: PMC5555743, DOI: 10.1007/s00251-017-0978-6.
- Canonical and Cross-reactive Binding of NK Cell Inhibitory Receptors to HLA-C Allotypes Is Dictated by Peptides Bound to HLA-CSim MJ, Malaker SA, Khan A, Stowell JM, Shabanowitz J, Peterson ME, Rajagopalan S, Hunt DF, Altmann DM, Long EO, Boyton RJ. Canonical and Cross-reactive Binding of NK Cell Inhibitory Receptors to HLA-C Allotypes Is Dictated by Peptides Bound to HLA-C. Frontiers In Immunology 2017, 8: 193. PMID: 28352266, PMCID: PMC5348643, DOI: 10.3389/fimmu.2017.00193.
- Identification and Characterization of Complex Glycosylated Peptides Presented by the MHC Class II Processing Pathway in MelanomaMalaker SA, Ferracane MJ, Depontieu FR, Zarling AL, Shabanowitz J, Bai DL, Topalian SL, Engelhard VH, Hunt DF. Identification and Characterization of Complex Glycosylated Peptides Presented by the MHC Class II Processing Pathway in Melanoma. Journal Of Proteome Research 2016, 16: 228-237. PMID: 27550523, PMCID: PMC5218890, DOI: 10.1021/acs.jproteome.6b00496.
- MHC Class I–Associated Phosphopeptides Are the Targets of Memory-like Immunity in LeukemiaCobbold M, De La Peña H, Norris A, Polefrone JM, Qian J, English AM, Cummings KL, Penny S, Turner JE, Cottine J, Abelin JG, Malaker SA, Zarling AL, Huang HW, Goodyear O, Freeman SD, Shabanowitz J, Pratt G, Craddock C, Williams ME, Hunt DF, Engelhard VH. MHC Class I–Associated Phosphopeptides Are the Targets of Memory-like Immunity in Leukemia. Science Translational Medicine 2013, 5: 203ra125. PMID: 24048523, PMCID: PMC4071620, DOI: 10.1126/scitranslmed.3006061.