2015
Tumor Necrosis Factor Disrupts Claudin-5 Endothelial Tight Junction Barriers in Two Distinct NF-κB-Dependent Phases
Clark PR, Kim RK, Pober JS, Kluger MS. Tumor Necrosis Factor Disrupts Claudin-5 Endothelial Tight Junction Barriers in Two Distinct NF-κB-Dependent Phases. PLOS ONE 2015, 10: e0120075. PMID: 25816133, PMCID: PMC4376850, DOI: 10.1371/journal.pone.0120075.Peer-Reviewed Original ResearchCell Membrane PermeabilityCells, CulturedClaudin-5DermisEndothelium, VascularHuman Umbilical Vein Endothelial CellsHumansMicroscopy, FluorescenceMyosin Light ChainsMyosin-Light-Chain KinaseNF-kappa BPhosphorylationRho-Associated KinasesRNA, Small InterferingSignal TransductionTight JunctionsTumor Necrosis Factor-alpha
2006
Increased ICAM-1 Expression Causes Endothelial Cell Leakiness, Cytoskeletal Reorganization and Junctional Alterations
Clark PR, Manes TD, Pober JS, Kluger MS. Increased ICAM-1 Expression Causes Endothelial Cell Leakiness, Cytoskeletal Reorganization and Junctional Alterations. Journal Of Investigative Dermatology 2006, 127: 762-774. PMID: 17195014, DOI: 10.1038/sj.jid.5700670.Peer-Reviewed Original ResearchConceptsEC barrier functionCell junctionsHuman dermal microvascular ECsSmall-interfering RNA knockdownEC shape changesEndothelial cellsG actin ratioActin cytoskeletonShape changesCytoskeleton organizationCell elongationICAM-1 inductionCytoskeletal reorganizationIntracellular regionCell shapeRNA knockdownHuman dermal microvascular endothelial cellsDermal microvascular endothelial cellsZona occludens-1NF-kappaB activationJunctional alterationsCytoskeletonBarrier functionCell leakinessMicrovascular endothelial cells