2023
Calreticulin Regulates SARS-CoV-2 Spike Protein Turnover and Modulates SARS-CoV-2 Infectivity
Rahimi N, White M, Amraei R, Lotfollahzadeh S, Xia C, Michalak M, Costello C, Mühlberger E. Calreticulin Regulates SARS-CoV-2 Spike Protein Turnover and Modulates SARS-CoV-2 Infectivity. Cells 2023, 12: 2694. PMID: 38067122, PMCID: PMC10705507, DOI: 10.3390/cells12232694.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectivityCardiovascular complicationsS-RBDSARS-CoV-2 infectionEndothelial cellsMajor clinical hallmarksSpike proteinCOVID-19 patientsCoronavirus disease 2019SARS-CoV-2 spike proteinSARS-CoV-2S proteinProteasomal inhibitor bortezomibHuman endothelial cellsShRNA-mediated knockdownCalcium hemostasisClinical hallmarkDisease 2019Inhibitor bortezomibCalcium homeostasisAcidification of lysosomesRole of calreticulinTreatment of cellsProtein levelsComplicationsInactivation of Minar2 in mice hyperactivates mTOR signaling and results in obesity
Lotfollahzadeh S, Xia C, Amraei R, Hua N, Kandror K, Farmer S, Wei W, Costello C, Chitalia V, Rahimi N. Inactivation of Minar2 in mice hyperactivates mTOR signaling and results in obesity. Molecular Metabolism 2023, 73: 101744. PMID: 37245847, PMCID: PMC10267597, DOI: 10.1016/j.molmet.2023.101744.Peer-Reviewed Original ResearchConceptsMTOR activationHigh-fat dietObesity-associated diseasesGlucose toleranceKO miceChronic diseasesPathophysiological roleBody fatMetabolic disordersHypertrophic adipocytesKnockout miceObesityAdipose tissuePhysiological negative regulatorType 2HEK-293 cellsImpaired expressionComplex disorderCell culture studiesAdipocytesDiseaseMiceDisordersMTORUnknown role
2022
PRMT4-mediated arginine methylation promotes tyrosine phosphorylation of VEGFR-2 and regulates filopodia protrusions
Hartsough E, Shelke R, Amraei R, Aryan Z, Lotfollahzadeh S, Rahimi N. PRMT4-mediated arginine methylation promotes tyrosine phosphorylation of VEGFR-2 and regulates filopodia protrusions. IScience 2022, 25: 104736. PMID: 35942094, PMCID: PMC9356023, DOI: 10.1016/j.isci.2022.104736.Peer-Reviewed Original ResearchSrc homology domain 2Protein arginine methyltransferase 4N-terminal domain bindsFilopodia protrusionsVEGFR-2Arginine methylationDomain bindsVascular endothelial growth factor receptor 2 activationSignal transductionTyrosine phosphorylationC-SrcMolecular mechanismsDomain 2MethylationPhosphorylationAngiogenic signalingPathological angiogenesisReceptor 2 activationAngiogenic responseTumor angiogenesisVEGFR-2 inhibitorsEVH1AngiogenesisTransductionSignalingCentral Gαi2 Protein Mediated Neuro-Hormonal Control of Blood Pressure and Salt Sensitivity
Amraei R, Moreira J, Wainford R. Central Gαi2 Protein Mediated Neuro-Hormonal Control of Blood Pressure and Salt Sensitivity. Frontiers In Endocrinology 2022, 13: 895466. PMID: 35837296, PMCID: PMC9275552, DOI: 10.3389/fendo.2022.895466.Peer-Reviewed Original ResearchConceptsDietary sodium intakeG protein-coupled receptorsParaventricular nucleusBlood pressureSodium intakeElectrolyte homeostasisCentral angiotensin II type 1 receptorsAcute isotonic volume expansionAngiotensin II type 1 receptorII type 1 receptorSalt sensitivityMajor public health issueAcute pharmacological activationAfferent renal nervesNeuro-hormonal responseSalt-sensitive animalsDevelopment of hypertensionSalt-resistant ratsIsotonic volume expansionHypothalamic paraventricular nucleusMultiple G protein-coupled receptorsType 1 receptorNeuro-hormonal controlGαi/oPublic health issueExtracellular vimentin is an attachment factor that facilitates SARS-CoV-2 entry into human endothelial cells
Amraei R, Xia C, Olejnik J, White M, Napoleon M, Lotfollahzadeh S, Hauser B, Schmidt A, Chitalia V, Mühlberger E, Costello C, Rahimi N. Extracellular vimentin is an attachment factor that facilitates SARS-CoV-2 entry into human endothelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2113874119. PMID: 35078919, PMCID: PMC8833221, DOI: 10.1073/pnas.2113874119.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionHuman endothelial cellsSARS-CoV-2 entrySARS-CoV-2S-protein interactionEndothelial cellsIdentification of vimentinShRNA-mediated knockdownIntermediate filament proteinsBinding of vimentinHEK-293 cellsAttachment factorsViral entrySARS-CoV-2 S proteinDevelopment of therapeuticsExtracellular vimentinS protein receptorInfectious SARS-CoV-2Host cellsCellular componentsCoexpression of vimentinFilament proteinsPrimary entry receptorSARS-CoV-2 spike proteinS protein
2021
The cell adhesion molecule TMIGD1 binds to moesin and regulates tubulin acetylation and cell migration
Rahimi N, Ho R, Chandler K, De La Cena K, Amraei R, Mitchel A, Engblom N, Costello C. The cell adhesion molecule TMIGD1 binds to moesin and regulates tubulin acetylation and cell migration. Journal Of Biomedical Science 2021, 28: 61. PMID: 34503512, PMCID: PMC8427838, DOI: 10.1186/s12929-021-00757-z.Peer-Reviewed Original ResearchConceptsMitotic spindle organizationERM familyΑ-tubulinSpindle organizationCell migrationApical localizationN-terminal ERM domainCRISPR/Cas9-mediated knockoutMitotic spindle assemblyCell-cell adhesionCas9-mediated knockoutFilopodia-like protrusionsNovel tumor suppressorComplex functional interplayActin organizationERM domainLysine acetylationTMIGD1Spindle assemblyCarboxyl terminusFunctional interplayMoesinCell cycleTumor suppressorEzrinCD209L/L-SIGN and CD209/DC-SIGN Act as Receptors for SARS-CoV‑2
Amraei R, Yin W, Napoleon M, Suder E, Berrigan J, Zhao Q, Olejnik J, Chandler K, Xia C, Feldman J, Hauser B, Caradonna T, Schmidt A, Gummuluru S, Mühlberger E, Chitalia V, Costello C, Rahimi N. CD209L/L-SIGN and CD209/DC-SIGN Act as Receptors for SARS-CoV‑2. ACS Central Science 2021, 7: 1156-1165. PMID: 34341769, PMCID: PMC8265543, DOI: 10.1021/acscentsci.0c01537.Peer-Reviewed Original ResearchSARS-CoV-2 spike receptor-binding domainSARS-CoV-2SARS-CoV-2 entryL-SIGNSARS-CoV-2 infectionCD209/DC-SIGNSpike receptor-binding domainAntiviral drug developmentReceptor-binding domainCell typesHuman endothelial cellsDC-SIGNAlternative receptorLow expressionEndothelial cellsImmunofluorescence stainingCD209Virus entryACE2Disease-relevant cell typesProminent expressionCD209LKidney epitheliumReceptorsDrug developmentNEDD4 regulates ubiquitination and stability of the cell adhesion molecule IGPR-1 via lysosomal pathway
Sun L, Amraei R, Rahimi N. NEDD4 regulates ubiquitination and stability of the cell adhesion molecule IGPR-1 via lysosomal pathway. Journal Of Biomedical Science 2021, 28: 35. PMID: 33962630, PMCID: PMC8103646, DOI: 10.1186/s12929-021-00731-9.Peer-Reviewed Original ResearchConceptsCell adhesion molecule IGPR-1IGPR-1Lysosomal-dependent degradationUbiquitin E3Vivo co-immunoprecipitation assaysWild-type Nedd4Knockdown of NEDD4Polyproline-rich motifCritical cellular processesCell-cell adhesionCo-immunoprecipitation assaysTreatment of cellsCell surface levelsHEK-293 cellsA375 melanoma cellsWW domainsCellular processesRich motifLysosomal pathwayC-terminusNedd4Key regulatorLysosomal inhibitorsMolecular mechanismsMelanoma cell lines
2020
Transmembrane and Immunoglobulin Domain Containing 1, a Putative Tumor Suppressor, Induces G2/M Cell Cycle Checkpoint Arrest in Colon Cancer Cells
De La Cena K, Ho R, Amraei R, Woolf N, Tashjian J, Zhao Q, Richards S, Walker J, Huang J, Chitalia V, Rahimi N. Transmembrane and Immunoglobulin Domain Containing 1, a Putative Tumor Suppressor, Induces G2/M Cell Cycle Checkpoint Arrest in Colon Cancer Cells. American Journal Of Pathology 2020, 191: 157-167. PMID: 33129760, PMCID: PMC7788663, DOI: 10.1016/j.ajpath.2020.09.015.Peer-Reviewed Original ResearchConceptsCell cycleColorectal cancerCell cycle checkpoint arrestCell cycle inhibitor proteinsNovel tumor suppressor geneFull molecular mechanismsPutative tumor suppressorColon cancer cellsTumor suppressor geneG2/M phaseSporadic human colorectal cancerNormal intestinal epithelial cellsEpigenetic mechanismsCheckpoint arrestTMIGD1Inhibitor proteinNovel potential therapeutic targetIntestinal epithelial cellsMolecular mechanismsTumor suppressorPoor overall survivalSuppressor geneDevelopment of adenomasImmunoglobulin domainHuman colorectal cancerCell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy
Amraei R, Alwani T, Ho R, Aryan Z, Wang S, Rahimi N. Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy. Journal Of Biological Chemistry 2020, 295: 16691-16699. PMID: 32978258, PMCID: PMC7864065, DOI: 10.1074/jbc.ra120.014790.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAMP-Activated Protein KinasesAnimalsAutophagyAutophagy-Related Protein-1 HomologBeclin-1CD28 AntigensCell AdhesionHEK293 CellsHumansI-kappa B KinaseIntracellular Signaling Peptides and ProteinsLipopolysaccharidesMicroscopy, FluorescenceMicrotubule-Associated ProteinsPhosphorylationPrimatesRNA, Guide, KinetoplastidaSirolimusSubstrate SpecificityConceptsIGPR-1Cell adhesionCell adhesion molecule IGPR-1Proline-rich receptor-1Serine/threonine kinaseKey serine/threonine kinaseAmino acid starvationBeclin-1Phosphorylation of AMPThreonine kinaseAutophagy stimuliKinase assaysLC3-II levelsCellular stressNutrient deprivationProtein kinaseCell adhesion moleculeProtein ULK1IκB kinase βEndothelial barrier functionKinase βCellular assaysPhosphorylationSubsequent activationCell detachmentCOVID-19, Renin-Angiotensin System and Endothelial Dysfunction
Amraei R, Rahimi N. COVID-19, Renin-Angiotensin System and Endothelial Dysfunction. Cells 2020, 9: 1652. PMID: 32660065, PMCID: PMC7407648, DOI: 10.3390/cells9071652.Peer-Reviewed Original ResearchConceptsRenin-angiotensin systemSARS-CoV-2Acute respiratory distress syndromeSARS-CoV-2 infectionEndothelial dysfunctionVascular damageCell adhesion moleculeSARS-CoV-2-induced acute respiratory distress syndromeCOVID-19Pre-existing endothelial dysfunctionSARS-CoV-2 entryRespiratory distress syndromeEndothelial cell injuryRole of ACE2Coronavirus disease 2019 (COVID-19) outbreakNovel coronavirus disease 2019 (COVID-19) outbreakCD209/DC-SIGNSARS-CoV-2 virusNew therapeutic strategiesDisease 2019 outbreakHuman vascular endotheliumGlobal public healthHost cellsAngiotensin fragmentsDistress syndromeThyroSeq v2 Testing: Impact on Cytologic Diagnosis, Management, and Cost of Care in Patients with Thyroid Nodule
Fazeli S, Zehr B, Amraei R, Toraldo G, Guan H, Kindelberger D, Lee S, Cerda S. ThyroSeq v2 Testing: Impact on Cytologic Diagnosis, Management, and Cost of Care in Patients with Thyroid Nodule. Thyroid 2020, 30: 1528-1534. PMID: 32349630, DOI: 10.1089/thy.2019.0191.Peer-Reviewed Original ResearchConceptsAUS/FLUSCost of careIndeterminate cytology resultsThyroid nodulesThyroSeq v2Cytology resultsCytologic diagnosisFN/SFN categoriesUndetermined significance/follicular lesionMolecular testsRate of overtreatmentRate of surgeryRate of malignancyOutcome of managementFollicular neoplasm/Fine-needle aspirationIndeterminate cytologic diagnosisOverall rateFN/SFNThyroid fine-needle aspirationNovel molecular testsThyroSeq testingLack of decreaseConsecutive patientsRetrospective studyLoss of MINAR2 impairs motor function and causes Parkinson’s disease-like symptoms in mice
Ho R, Amraei R, De La Cena K, Sutherland E, Mortazavi F, Stein T, Chitalia V, Rahimi N. Loss of MINAR2 impairs motor function and causes Parkinson’s disease-like symptoms in mice. Brain Communications 2020, 2: fcaa047. PMID: 32954300, PMCID: PMC7425422, DOI: 10.1093/braincomms/fcaa047.Peer-Reviewed Original ResearchReceptor 2Parkinson's spectrum disordersDisease-like symptomsPars compactaΑ-synuclein protein expressionTyrosine hydroxylase-positive neuronsSubstantia nigra pars compactaFrontal lobe brainParkinson's disease-like symptomsLewy body dementiaImpairs motor functionSevere motor deficitsPrimary clinical symptomsReceptor 2 expressionKnockout mouse brainMotor control impairmentsSpectrum disorderCommon human neurodegenerative diseasesΑ-synuclein aggregationMotor deficitsClinical symptomsDopaminergic neuronsSpontaneous locomotorClinical hallmarkMotor function
2019
c-Cbl targets PD-1 in immune cells for proteasomal degradation and modulates colorectal tumor growth
Lyle C, Richards S, Yasuda K, Napoleon M, Walker J, Arinze N, Belghasem M, Vellard I, Yin W, Ravid J, Zavaro E, Amraei R, Francis J, Phatak U, Rifkin I, Rahimi N, Chitalia V. c-Cbl targets PD-1 in immune cells for proteasomal degradation and modulates colorectal tumor growth. Scientific Reports 2019, 9: 20257. PMID: 31882749, PMCID: PMC6934810, DOI: 10.1038/s41598-019-56208-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8-Positive T-LymphocytesColorectal NeoplasmsGene Expression Regulation, NeoplasticHumansMacrophagesMice, KnockoutPhosphorylationProgrammed Cell Death 1 ReceptorProteasome Endopeptidase ComplexProto-Oncogene Proteins c-cblTumor BurdenTumor MicroenvironmentUbiquitinationUbiquitin-Protein LigasesConceptsPD-1Immune cellsTumor microenvironmentColorectal cancerCell death 1 proteinPD-1 regulationDeath 1 proteinImmune checkpoint receptorsColorectal tumor growthC-CblCasitas B lymphomaCRC xenograftsImmune checkpointsCheckpoint receptorsAutoimmune diseasesT lymphocytesTumor suppressive activitySuppressive activityTumor phagocytosisTumor growthFinger functionB lymphomaHigh expressionMiceXenograftsThe cell adhesion molecule IGPR-1 is activated by and regulates responses of endothelial cells to shear stress
Ho R, Tahboub R, Amraei R, Meyer R, Varongchayakul N, Grinstaff M, Rahimi N. The cell adhesion molecule IGPR-1 is activated by and regulates responses of endothelial cells to shear stress. Journal Of Biological Chemistry 2019, 294: 13671-13680. PMID: 31341021, PMCID: PMC6746441, DOI: 10.1074/jbc.ra119.008548.Peer-Reviewed Original ResearchConceptsIGPR-1Cell adhesion molecule IGPR-1Proline-rich receptor-1AKT Ser/ThrActin stress fiber assemblySer/ThrCell-cell junctionsStress fiber assemblyCell-cell contactEndothelial cellsActin fiber assemblyEndothelial cell remodelingFiber assemblyCell mechanosensingBlood flow-induced shear stressCell remodelingCellular responsesMechanical signalsVascular endothelial cellsImportant playersFlow-induced shear stressPhosphorylationCellsReceptor 1Cell borders
2018
Zolpidem-induced sneezing
Amraei R, Parsa A, Babaeian M. Zolpidem-induced sneezing. Medicine 2018, 97: e9918. PMID: 29489694, PMCID: PMC5851732, DOI: 10.1097/md.0000000000009918.Peer-Reviewed Original ResearchEffects of Heparin on Early Patency of Arteriovenous Fistula in Angioaccess Surgery of Patients with End-Stage Renal Disease
Mozafar M, Hoseinzadegan F, Lotfollahzadeh S, Baikpour M, Amraei R, Solatpour F, Baikpour M. Effects of Heparin on Early Patency of Arteriovenous Fistula in Angioaccess Surgery of Patients with End-Stage Renal Disease. Internal Medicine And Medical Investigation Journal 2018, 3: 36-39. DOI: 10.24200/imminv.v2i4.108.Peer-Reviewed Original ResearchArteriovenous fistulaSystemic anticoagulationPatency ratesVascular accessHeparin injectionControl groupEnd-stage renal disease patientsPatency of AVFPermanent vascular access placementEnd-stage renal diseaseAVF patency ratesVascular access placementRenal disease patientsVascular access failureAngioaccess surgeryAVF patencyRenal diseaseAccess placementEarly patencyIntraoperative anticoagulationTajrish HospitalLarge trialsEarly thrombosisAV fistulaDisease patients
2017
A Comparison between the Diagnostic Accuracy of Frozen Section and Permanent Section Analyses in Central Nervous System
Amraei R, Moradi A, Zham H, Ahadi M, Baikpour M, Rakhshan A. A Comparison between the Diagnostic Accuracy of Frozen Section and Permanent Section Analyses in Central Nervous System. Asian Pacific Journal Of Cancer Prevention 2017, 18: 659-666. PMID: 28440972, PMCID: PMC5464481, DOI: 10.22034/apjcp.2017.18.3.659.Peer-Reviewed Original ResearchCentral nervous system tumorsNervous system tumorsCentral nervous systemFrozen sectionsSystem tumorsNervous systemPermanent section analysisDescriptive retrospective studyIntra-operative diagnosisCNS surgeryCNS lesionsPathologic evaluationRadiologic findingsCNS tumorsRetrospective studyHistopathological evaluationClinical historyDefinite diagnosisMedical recordsPathology reportsDiagnostic acuityPosterior fossaTumor categoryMiddle fossaPatients
2015
In the Era of Shared Decision Making, How Would An Iranian Urologist Screen Himself For Prostate Cancer?
Ali Asgari M, Soleimani M, Dadkhah F, Lashay A, Amini E, Baikpour M, Amraei R. In the Era of Shared Decision Making, How Would An Iranian Urologist Screen Himself For Prostate Cancer? Urology Journal 2015, 12: 2404-9. PMID: 26706736.Peer-Reviewed Original ResearchConceptsProstate-specific antigenDigital rectal examinationProstate cancerScreening programSerial prostate-specific antigenLarger high-quality studiesProstate-specific mortalityHigh-quality studiesSignificant health problemUrologists' attitudesCause mortalityPSA levelsLarge trialsRectal examinationCurrent guidelinesUrological AssociationNormal rangeSpecific antigenHealth problemsUrologistsPatientsSignificant reductionCancerMortalityQuestionnaire-based survey
2014
Comparison of the Incidence of Sore Throat After Rapid Sequence Intubation With Succinylcholine and Cisatracurium
Solatpour F, Teymourian H, Mohajerani S, Shirazi F, Zadeh S, Baikpour M, Amraei R. Comparison of the Incidence of Sore Throat After Rapid Sequence Intubation With Succinylcholine and Cisatracurium. Anesthesiology And Pain Medicine 2014, 4: e20030. PMID: 25289376, PMCID: PMC4183082, DOI: 10.5812/aapm.20030.Peer-Reviewed Original ResearchPostoperative sore throatSore throatRapid sequence intubationSequence intubationInduction agentPost-operative sore throatEmergent abdominal surgeryHours post operationRapid sequence inductionNumber of patientsCisatracurium groupSuccinylcholine groupAbdominal surgeryCommon complicationRecovery roomTajrish HospitalAirway managementEndotracheal intubationSequence inductionMuscle achesPost operationDry throatPhysician's choicePatientsSuccinylcholine