Razie Amraei, MD, PhD
Hospital ResidentCards
About
Research
Publications
2025
110 Characterization of Frequency and Spectrum of PIK3CA Somatic Mutations in Advanced Breast Cancer
Amraei R, Kahn A, Walther Z, Finberg K, Gibson J, Kahila M. 110 Characterization of Frequency and Spectrum of PIK3CA Somatic Mutations in Advanced Breast Cancer. Laboratory Investigation 2025, 105: 102334. DOI: 10.1016/j.labinv.2024.102334.Peer-Reviewed Original Research340 Cytomorphologic Challenges in Cervical Pap Tests of Transgender Men on Androgen Therapy: A Cyto-Histological Correlation Study
Haque M, Amraei R, Iyer K, Krishnamurti U, Adeniran A, Sun T. 340 Cytomorphologic Challenges in Cervical Pap Tests of Transgender Men on Androgen Therapy: A Cyto-Histological Correlation Study. Laboratory Investigation 2025, 105: 102567. DOI: 10.1016/j.labinv.2024.102567.Peer-Reviewed Original Research
2024
Proteomic and phosphoproteomic identified structural and functional changes in the aorta associate with age-dependent hypertension in male Sprague-Dawley rats
Amraei R, Lampl N, Nist K, Zhang Y, Wainford R. Proteomic and phosphoproteomic identified structural and functional changes in the aorta associate with age-dependent hypertension in male Sprague-Dawley rats. Physiological Genomics 2024, 57: 16-27. PMID: 39548828, DOI: 10.1152/physiolgenomics.00052.2024.Peer-Reviewed Original ResearchIncreased risk of HFRisk of HFMendelian randomizationMR analysisCardiovascular diseaseTwo-sample bidirectional Mendelian randomizationBidirectional MR analysisBidirectional Mendelian randomizationInstrumental variablesIncreased riskUnited Kingdom BiobankCoronary artery diseaseCausal effectsHorizontal pleiotropyPleiotropy testConfounding factorsHeart failureObservational studyGenetic variantsAtrial fibrillationPotential associationManagement of patientsEuropean populationsClinical management of patientsReverse causal effect
2023
Calreticulin Regulates SARS-CoV-2 Spike Protein Turnover and Modulates SARS-CoV-2 Infectivity
Rahimi N, White M, Amraei R, Lotfollahzadeh S, Xia C, Michalak M, Costello C, Mühlberger E. Calreticulin Regulates SARS-CoV-2 Spike Protein Turnover and Modulates SARS-CoV-2 Infectivity. Cells 2023, 12: 2694. PMID: 38067122, PMCID: PMC10705507, DOI: 10.3390/cells12232694.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectivityCardiovascular complicationsS-RBDSARS-CoV-2 infectionEndothelial cellsMajor clinical hallmarksSpike proteinCOVID-19 patientsCoronavirus disease 2019SARS-CoV-2 spike proteinSARS-CoV-2S proteinProteasomal inhibitor bortezomibHuman endothelial cellsShRNA-mediated knockdownCalcium hemostasisClinical hallmarkDisease 2019Inhibitor bortezomibCalcium homeostasisAcidification of lysosomesRole of calreticulinTreatment of cellsProtein levelsComplicationsInactivation of Minar2 in mice hyperactivates mTOR signaling and results in obesity
Lotfollahzadeh S, Xia C, Amraei R, Hua N, Kandror K, Farmer S, Wei W, Costello C, Chitalia V, Rahimi N. Inactivation of Minar2 in mice hyperactivates mTOR signaling and results in obesity. Molecular Metabolism 2023, 73: 101744. PMID: 37245847, PMCID: PMC10267597, DOI: 10.1016/j.molmet.2023.101744.Peer-Reviewed Original ResearchConceptsMTOR activationHigh-fat dietObesity-associated diseasesGlucose toleranceKO miceChronic diseasesPathophysiological roleBody fatMetabolic disordersHypertrophic adipocytesKnockout miceObesityAdipose tissuePhysiological negative regulatorType 2HEK-293 cellsImpaired expressionComplex disorderCell culture studiesAdipocytesDiseaseMiceDisordersMTORUnknown role
2022
PRMT4-mediated arginine methylation promotes tyrosine phosphorylation of VEGFR-2 and regulates filopodia protrusions
Hartsough E, Shelke R, Amraei R, Aryan Z, Lotfollahzadeh S, Rahimi N. PRMT4-mediated arginine methylation promotes tyrosine phosphorylation of VEGFR-2 and regulates filopodia protrusions. IScience 2022, 25: 104736. PMID: 35942094, PMCID: PMC9356023, DOI: 10.1016/j.isci.2022.104736.Peer-Reviewed Original ResearchSrc homology domain 2Protein arginine methyltransferase 4N-terminal domain bindsFilopodia protrusionsVEGFR-2Arginine methylationDomain bindsVascular endothelial growth factor receptor 2 activationSignal transductionTyrosine phosphorylationC-SrcMolecular mechanismsDomain 2MethylationPhosphorylationAngiogenic signalingPathological angiogenesisReceptor 2 activationAngiogenic responseTumor angiogenesisVEGFR-2 inhibitorsEVH1AngiogenesisTransductionSignalingCentral Gαi2 Protein Mediated Neuro-Hormonal Control of Blood Pressure and Salt Sensitivity
Amraei R, Moreira J, Wainford R. Central Gαi2 Protein Mediated Neuro-Hormonal Control of Blood Pressure and Salt Sensitivity. Frontiers In Endocrinology 2022, 13: 895466. PMID: 35837296, PMCID: PMC9275552, DOI: 10.3389/fendo.2022.895466.Peer-Reviewed Original ResearchConceptsDietary sodium intakeG protein-coupled receptorsParaventricular nucleusBlood pressureSodium intakeElectrolyte homeostasisCentral angiotensin II type 1 receptorsAcute isotonic volume expansionAngiotensin II type 1 receptorII type 1 receptorSalt sensitivityMajor public health issueAcute pharmacological activationAfferent renal nervesNeuro-hormonal responseSalt-sensitive animalsDevelopment of hypertensionSalt-resistant ratsIsotonic volume expansionHypothalamic paraventricular nucleusMultiple G protein-coupled receptorsType 1 receptorNeuro-hormonal controlGαi/oPublic health issueExtracellular vimentin is an attachment factor that facilitates SARS-CoV-2 entry into human endothelial cells
Amraei R, Xia C, Olejnik J, White M, Napoleon M, Lotfollahzadeh S, Hauser B, Schmidt A, Chitalia V, Mühlberger E, Costello C, Rahimi N. Extracellular vimentin is an attachment factor that facilitates SARS-CoV-2 entry into human endothelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2113874119. PMID: 35078919, PMCID: PMC8833221, DOI: 10.1073/pnas.2113874119.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionHuman endothelial cellsSARS-CoV-2 entrySARS-CoV-2S-protein interactionEndothelial cellsIdentification of vimentinShRNA-mediated knockdownIntermediate filament proteinsBinding of vimentinHEK-293 cellsAttachment factorsViral entrySARS-CoV-2 S proteinDevelopment of therapeuticsExtracellular vimentinS protein receptorInfectious SARS-CoV-2Host cellsCellular componentsCoexpression of vimentinFilament proteinsPrimary entry receptorSARS-CoV-2 spike proteinS protein
2021
The cell adhesion molecule TMIGD1 binds to moesin and regulates tubulin acetylation and cell migration
Rahimi N, Ho R, Chandler K, De La Cena K, Amraei R, Mitchel A, Engblom N, Costello C. The cell adhesion molecule TMIGD1 binds to moesin and regulates tubulin acetylation and cell migration. Journal Of Biomedical Science 2021, 28: 61. PMID: 34503512, PMCID: PMC8427838, DOI: 10.1186/s12929-021-00757-z.Peer-Reviewed Original ResearchConceptsMitotic spindle organizationERM familyΑ-tubulinSpindle organizationCell migrationApical localizationN-terminal ERM domainCRISPR/Cas9-mediated knockoutMitotic spindle assemblyCell-cell adhesionCas9-mediated knockoutFilopodia-like protrusionsNovel tumor suppressorComplex functional interplayActin organizationERM domainLysine acetylationTMIGD1Spindle assemblyCarboxyl terminusFunctional interplayMoesinCell cycleTumor suppressorEzrinCD209L/L-SIGN and CD209/DC-SIGN Act as Receptors for SARS-CoV‑2
Amraei R, Yin W, Napoleon M, Suder E, Berrigan J, Zhao Q, Olejnik J, Chandler K, Xia C, Feldman J, Hauser B, Caradonna T, Schmidt A, Gummuluru S, Mühlberger E, Chitalia V, Costello C, Rahimi N. CD209L/L-SIGN and CD209/DC-SIGN Act as Receptors for SARS-CoV‑2. ACS Central Science 2021, 7: 1156-1165. PMID: 34341769, PMCID: PMC8265543, DOI: 10.1021/acscentsci.0c01537.Peer-Reviewed Original ResearchSARS-CoV-2 spike receptor-binding domainSARS-CoV-2SARS-CoV-2 entryL-SIGNSARS-CoV-2 infectionCD209/DC-SIGNSpike receptor-binding domainAntiviral drug developmentReceptor-binding domainCell typesHuman endothelial cellsDC-SIGNAlternative receptorLow expressionEndothelial cellsImmunofluorescence stainingCD209Virus entryACE2Disease-relevant cell typesProminent expressionCD209LKidney epitheliumReceptorsDrug development