Nadia Ameen, MBBS
Professor of Pediatrics (Gastroenterology)Cards
Appointments
Additional Titles
Affiliated Faculty, Yale Institute for Global Health
Contact Info
Pediatric Gastroenterology & Hepatology
PO Box 208064
New Haven, CT 06520-8064
United States
Appointments
Additional Titles
Affiliated Faculty, Yale Institute for Global Health
Contact Info
Pediatric Gastroenterology & Hepatology
PO Box 208064
New Haven, CT 06520-8064
United States
Appointments
Additional Titles
Affiliated Faculty, Yale Institute for Global Health
Contact Info
Pediatric Gastroenterology & Hepatology
PO Box 208064
New Haven, CT 06520-8064
United States
About
Titles
Professor of Pediatrics (Gastroenterology)
Affiliated Faculty, Yale Institute for Global Health
Biography
I am a URM physician-scientist and Professor of Pediatrics (Gastroenterology), Cellular and Molecular Physiology at the Yale University School of Medicine. I have led an NIH-supported laboratory for over 2 decades and trained multiple undergraduate students, post-docs, medical students and research scientists, the majority of whom come from under-represented backgrounds. My research interest is focused on mechanisms responsible for diarrheal diseases. My lab primarily investigates mechanisms regulating the CFTR chloride channel in the intestine and how these are linked to genetic, and non-genetic diarrheal diseases and Cystic Fibrosis (CF). We elucidated trafficking mechanisms regulating CFTR that are implicated in diarrhea that are the basis for successful drug therapies to treat constipation and increase intestinal fluidity (Linaclotide, Lubiprostone). Currently, we investigate kinase signaling mechanisms responsible for regulating CFTR in genetic and non genetic diarrheal diseases and CF affecting newborns and children.
My clinical practice is focused on food and gut health in children to treat and prevent obesity, and chronic lifestyle diseases. We promote the use of healthy food for prevention of intestinal diseases in children, provide nutritional consultation, and design culturally sensitive diets for parents. We provide conventional standard of care along side nutritional promotion as needed, but focus on foods, exercise, stress reduction and lifestyle as a primary modalities for disease treatment and prevention.
Appointments
Pediatric Gastroenterology & Hepatology
ProfessorPrimaryCellular & Molecular Physiology
ProfessorSecondary
Other Departments & Organizations
- Ameen Lab
- Cellular & Molecular Physiology
- Discovery to Cure Internship
- Molecular Medicine, Pharmacology, and Physiology
- Pediatric Colorectal Disorders Program
- Pediatric Gastroenterology & Hepatology
- Pediatric Healthy Gut & Constipation Program
- Pediatrics
- Yale Combined Program in the Biological and Biomedical Sciences (BBS)
- Yale Institute for Global Health
- Yale Ventures
Education & Training
- MBBS
- University of West Indies (1985)
Research
Overview
Our early studies focussed on identification of trafficking as a major mechanism regulating CFTR in the intestine and its relevance to secretory diarrhea. More recently, we investigate a rare genetic diarrheal disease that affect new borns, Microvillus Inclusion Disease(MVID). We were first to show that MVID results from an apical trafficking defect. Current investigations are elucidating kinase signaling mechanisms regulating ion transport that result in diarrhea in MVID.
Medical Subject Headings (MeSH)
- View Lab Website
Ameen Lab
Research at a Glance
Yale Co-Authors
Publications Timeline
Kaelyn Sumigray, PhD
John Geibel, DSc, MD, AGAF, MS, FRS
Alannah Garrison
Amos Brooks, HT (ASCP)
Mark Mooseker, PhD
Timothy Nottoli, PhD
Publications
2024
Localization and function of humanized F508del-CFTR in mouse intestine following activation of serum glucocorticoid kinase 1 and Trikafta
Dastoor P, Muiler C, Garrison A, Egan M, Carlos Dos Reis D, Santos A, Ameen N. Localization and function of humanized F508del-CFTR in mouse intestine following activation of serum glucocorticoid kinase 1 and Trikafta. European Journal Of Pharmacology 2024, 978: 176771. PMID: 38925289, DOI: 10.1016/j.ejphar.2024.176771.Peer-Reviewed Original ResearchConceptsSerum glucocorticoid kinase 1Glucocorticoid kinase 1F508del-CFTRCystic fibrosisMouse modelF508del-CFTR miceCFTR-expressing epithelial cellsCF mouse modelsHumanized mouse modelTreatment of ratsIntestinal diseaseIntestinal segmentsKinase 1CFTR modulatorsCFTR mutationsCF patientsTrikaftaDex treatmentLung diseaseEfficacy of compoundsDelta F508CFTRCombined treatmentEpithelial cellsLoss of functionSu1930 STRESS-INDUCED SIGNALING AND CFTR IN SGK1 KNOCK OUT MOUSE INTESTINE
Muiler C, Santos A, Dastoor P, Jin J, Reis D, Kazi M, Ameen N. Su1930 STRESS-INDUCED SIGNALING AND CFTR IN SGK1 KNOCK OUT MOUSE INTESTINE. Gastroenterology 2024, 166: s-874. DOI: 10.1016/s0016-5085(24)02489-2.Peer-Reviewed Original Research978 CFTR HIGH EXPRESSER CELLS EXPRESS NEUROPOD GENES AND RESPOND TO ENVIRONMENTAL CUES IN THE INTESTINE
Jin J, Reis D, Muiler C, Sumigray K, Ameen N. 978 CFTR HIGH EXPRESSER CELLS EXPRESS NEUROPOD GENES AND RESPOND TO ENVIRONMENTAL CUES IN THE INTESTINE. Gastroenterology 2024, 166: s-236. DOI: 10.1016/s0016-5085(24)01027-8.Peer-Reviewed Original Research
2023
555 Elexacaftor-tezacaftor-ivacaftor and serum glucocorticoid kinase 1 activation on humanized dF508 mouse intestine
Ameen N, Dastoor P, dos Reis D, Barone C, Garrison A, Egan M. 555 Elexacaftor-tezacaftor-ivacaftor and serum glucocorticoid kinase 1 activation on humanized dF508 mouse intestine. Journal Of Cystic Fibrosis 2023, 22: s293-s294. DOI: 10.1016/s1569-1993(23)01478-9.Peer-Reviewed Original ResearchConcepts539 Role of CFTR high expresser cells in cystic fibrosis intestinal disease
Ameen N, dos Reis D, Dastoor P, Santos A, Cohen M, Donnelley M, Parsons D. 539 Role of CFTR high expresser cells in cystic fibrosis intestinal disease. Journal Of Cystic Fibrosis 2023, 22: s285. DOI: 10.1016/s1569-1993(23)01462-5.Peer-Reviewed Original ResearchCFTR High Expresser Cells in cystic fibrosis and intestinal diseases
dos Reis D, Dastoor P, Santos A, Sumigray K, Ameen N. CFTR High Expresser Cells in cystic fibrosis and intestinal diseases. Heliyon 2023, 9: e14568. PMID: 36967909, PMCID: PMC10031467, DOI: 10.1016/j.heliyon.2023.e14568.Peer-Reviewed Original ResearchCitationsConceptsCystic fibrosisIntestinal diseaseCystic fibrosis transmembrane conductance regulatorQuality of lifeIntestinal dysfunctionGastrointestinal diseasesIntestinal physiologyFibrosisDiseasePathophysiologyFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCFTR leadConductance regulator
2022
Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID)
Ahsan K, dos Reis DC, Barbieri A, Sumigray KD, Nottoli T, Salas PJ, Ameen NA. Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID). Journal Of Clinical Medicine 2022, 11: 4179. PMID: 35887942, PMCID: PMC9319011, DOI: 10.3390/jcm11144179.Peer-Reviewed Original ResearchCitationsAltmetricConceptsMicrovillus inclusion diseaseSevere diarrheaInclusion diseaseFluid secretionCystic fibrosis transmembrane conductance regulatorDouble knockout miceMicrovillus inclusionsGlucocorticoid-inducible kinase 1Carbohydrate malabsorptionKnockout miceIntestinal carbohydrateDiarrheaDiarrheal diseaseMalabsorptionMiceVillus enterocytesDiseaseLoss of MYO5BFunction mutationsApical cystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorKinase 1SecretionIntestineTransmembrane conductance regulatorElevated 5‐hydroxytryptamine in COVID‐19 Stimulates ANO1 Mediated Cl Secretion in Lung & Intestinal Epithelial Cells
Hoque K, Hayashi M, Sheikh I, Banerjee A, Verma S, Leblanc N, Zeiss C, Ameen N, Chakraborty S. Elevated 5‐hydroxytryptamine in COVID‐19 Stimulates ANO1 Mediated Cl Secretion in Lung & Intestinal Epithelial Cells. The FASEB Journal 2022, 36: 10.1096/fasebj.2022.36.s1.0r556. PMCID: PMC9348250, DOI: 10.1096/fasebj.2022.36.s1.0r556.Peer-Reviewed Original ResearchAltmetricConceptsEnterochromaffin cell hyperplasiaCalu-3 cellsSARS-CoV2 infectionCl(-) secretionIntestinal epitheliumCell hyperplasiaColonic tissueCalcium-activated Cl- currentsCOVID-19-associated diarrheaCalcium-activated chloride currentsHuman colorectal cancer tissuesANO1 protein expressionCOVID-19 therapyIntestinal Cl(-) secretionColorectal cancer tissuesUssing chamber experimentsANO1 currentsSeverity of symptomsCOVID-19Human terminal ileumHuman intestinal epitheliumIntestinal epithelial cellsWhole-cell currentsHuman colonic tissueCaco-2 cellsSerum and Glucocorticoid‐Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID)
Ahsan K, dos Reis D, Barbieri A, Sumigray K, Nottoli T, Salas P, Ameen N. Serum and Glucocorticoid‐Inducible Kinase 1 (SGK1): An Important Contributor to Diarrhea and Malabsorption in Microvillus Inclusion Disease (MVID). The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.r5730.Peer-Reviewed Original ResearchAltmetricConceptsMicrovillus inclusion diseaseGlucocorticoid-inducible kinase 1Severe diarrheaF miceInclusion diseaseCystic fibrosis transmembrane conductance regulatorFluid secretionWorsening of diarrheaMicrovillus inclusionsPhosphorylation of PDK1Kinase 1Intestinal lysatesSGK1 pathwayCarbohydrate malabsorptionER miceTamoxifen inductionBeta-ENaCDiarrheaDiarrheal diseaseSmall intestineMalabsorptionMiceDCKO miceGlucose lossPhosphorylation of SGK1
2021
Intestinal TMEM16A control luminal chloride secretion in a NHERF1 dependent manner
Saha T, Aoun J, Hayashi M, Ali I, Sarkar P, Bag PK, Leblanc N, Ameen N, Woodward OM, Hoque KM. Intestinal TMEM16A control luminal chloride secretion in a NHERF1 dependent manner. Biochemistry And Biophysics Reports 2021, 25: 100912. PMID: 33537462, PMCID: PMC7838733, DOI: 10.1016/j.bbrep.2021.100912.Peer-Reviewed Original ResearchCitationsAltmetricConcepts
Academic Achievements & Community Involvement
activity Physiological Reports
Journal ServiceAssociate EditorDetailsAssociate Editor-Gastointestinal Physiology10/01/2018 - 11/02/2020honor NASPGHAN Award for Study of Disorders Associated with Carbohydrate Malabsorption
National AwardNorth American Society Pediatric GastroenterologyDetails10/07/2020United States
News & Links
Media
- We were first to show how apical ion transporters are dysregulated in MVID to account for diarrhea in this disease
- We use human intestinal biopsies to culture organoids, that are used to investigate CFTR and other epithelial transporters
News
- June 10, 2024
Yale Child Study Center welcomes 2024 summer interns
- July 16, 2020Source: American Journal of Physiology-Gastrointestinal and Liver Physiology
Glucocorticoids and serum- and glucocorticoid-inducible kinase 1 are potent regulators of CFTR in the native intestine: implications for stress-induced diarrhea
Get In Touch
Contacts
Pediatric Gastroenterology & Hepatology
PO Box 208064
New Haven, CT 06520-8064
United States
Locations
The Anlyan Center
Lab
300 Cedar Street, Ste S-160
New Haven, CT 06519
Appointments
203.737.4876