Sathish Ramakrishnan, PhD
Assistant Professor
Research & Publications
Biography
News
Research Summary
My research focuses on understanding the cellular and molecular mechanisms of fusion proteins, which mediate a wide range of cellular processes, including synaptic vesicle fusion, cell growth, cytokinesis, cancer signaling, progression, and onset. We quantitively dissect the role of SNAREs, and their regulators in normal and diseased conditions using engineered suspended lipid bilayer platforms and single-molecule imaging methods. Understanding the key players of exocytosis can drive therapeutic discovery by clarifying which proteins are critical for each step in the signaling pathway and block the disease progression.
Coauthors
Selected Publications
- PRRT2 Regulates Synaptic Fusion by Directly Modulating SNARE Complex AssemblyColeman J, Jouannot O, Ramakrishnan SK, Zanetti MN, Wang J, Salpietro V, Houlden H, Rothman JE, Krishnakumar SS. PRRT2 Regulates Synaptic Fusion by Directly Modulating SNARE Complex Assembly. Cell Reports 2018, 22: 820-831. PMID: 29346777, PMCID: PMC5792450, DOI: 10.1016/j.celrep.2017.12.056.
- Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosisRamakrishnan S, Bera M, Coleman J, Rothman JE, Krishnakumar SS. Synergistic roles of Synaptotagmin-1 and complexin in calcium-regulated neuronal exocytosis. ELife 2020, 9: e54506. PMID: 32401194, PMCID: PMC7220375, DOI: 10.7554/elife.54506.
- Molecular determinants of complexin clamping and activation functionBera M, Ramakrishnan S, Coleman J, Krishnakumar SS, Rothman JE. Molecular determinants of complexin clamping and activation function. ELife 2022, 11: e71938. PMID: 35442188, PMCID: PMC9020821, DOI: 10.7554/elife.71938.
- Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a ChipSundaram R, Bera M, Coleman J, Weerakkody J, Krishnakumar S, Ramakrishnan S. Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip. Small 2022, 18: e2205567. PMID: 36328714, DOI: 10.1002/smll.202205567.
- Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinerySundaram R, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst A, Gupta K, Rothman J, Krishnakumar S. Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2309516120. PMID: 37590407, PMCID: PMC10450444, DOI: 10.1073/pnas.2309516120.
- Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin TherapyThoduvayil S, Weerakkody J, Sundaram R, Topper M, Bera M, Coleman J, Li X, Mariappan M, Ramakrishnan S. Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy. Cell Calcium 2023, 113: 102766. PMID: 37295201, PMCID: PMC10450995, DOI: 10.1016/j.ceca.2023.102766.
- Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayerPanda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.
- Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip (Small 51/2022)Sundaram R, Bera M, Coleman J, Weerakkody J, Krishnakumar S, Ramakrishnan S. Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip (Small 51/2022). Small 2022, 18 DOI: 10.1002/smll.202270277.
- Suspended Lipid Bilayer: A Versatile Platform for Nextgen Drug Discovery and Biomedical ApplicationsRamakrishnan S, Weerakkody J. Suspended Lipid Bilayer: A Versatile Platform for Nextgen Drug Discovery and Biomedical Applications. Accounts Of Materials Research 2022, 3: 996-998. DOI: 10.1021/accountsmr.2c00157.
- Vesicle capture by membrane‐bound Munc13‐1 requires self‐assembly into discrete clustersLi F, Sundaram R, Gatta AT, Coleman J, Ramakrishnan S, Krishnakumar SS, Pincet F, Rothman JE. Vesicle capture by membrane‐bound Munc13‐1 requires self‐assembly into discrete clusters. FEBS Letters 2021, 595: 2185-2196. PMID: 34227103, DOI: 10.1002/1873-3468.14157.
- Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D‐Printed Microfluidic SetupHeo P, Ramakrishnan S, Coleman J, Rothman JE, Fleury J, Pincet F. Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D‐Printed Microfluidic Setup. Small 2019, 15: e1900725. PMID: 30977975, DOI: 10.1002/smll.201900725.
- Synaptotagmin oligomers are necessary and can be sufficient to form a Ca2+‐sensitive fusion clampRamakrishnan S, Bera M, Coleman J, Krishnakumar SS, Pincet F, Rothman JE. Synaptotagmin oligomers are necessary and can be sufficient to form a Ca2+‐sensitive fusion clamp. FEBS Letters 2019, 593: 154-162. PMID: 30570144, PMCID: PMC6349546, DOI: 10.1002/1873-3468.13317.
- High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated AnalysisRamakrishnan S, Gohlke A, Li F, Coleman J, Xu W, Rothman JE, Pincet F. High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis. Langmuir 2018, 34: 5849-5859. PMID: 29694054, DOI: 10.1021/acs.langmuir.8b00116.
- Ellipsometric study of peptide layers – island-like character, depolarization and quasi-absorptionPápa Z, Ramakrishnan S, Martin M, Cloitre T, Zimányi L, Tóth Z, Gergely C, Budai J. Ellipsometric study of peptide layers – island-like character, depolarization and quasi-absorption. Applied Surface Science 2017, 421: 707-713. DOI: 10.1016/j.apsusc.2017.02.263.
- Cover Image, Volume 7, Issue 1Ramakrishnan S, Zhu J, Gergely C. Cover Image, Volume 7, Issue 1. Wiley Interdisciplinary Reviews Computational Molecular Science 2017, 7 DOI: 10.1002/wcms.1302.
- Organic–inorganic interface simulation for new material discoveriesRamakrishnan SK, Zhu J, Gergely C, Wiley Interdisciplinary Reviews: Computational Molecular Science 2017, 7, e1277.
- Ellipsometric study of peptide layers–island-like character, depolarization and quasi-absorptionPápa Z, Ramakrishnan S, Martin M, Cloitre T, Zimányi L, Tóth L, Gergely C, Budai J, Applied Surface Science 2017, 421, 707–713
- Organic–inorganic interface simulation for new material discoveriesRamakrishnan S, Zhu J, Gergely C. Organic–inorganic interface simulation for new material discoveries. Wiley Interdisciplinary Reviews Computational Molecular Science 2016, 7 DOI: 10.1002/wcms.1277.
- Interactions at the Peptide/Silicon Surfaces: Evidence of Peptide Multilayer AssemblyPápa Z, Ramakrishnan SK, Martin M, Cloitre T, Zimányi L, Márquez J, Budai J, Tóth Z, Gergely C. Interactions at the Peptide/Silicon Surfaces: Evidence of Peptide Multilayer Assembly. Langmuir 2016, 32: 7250-7258. PMID: 27315212, DOI: 10.1021/acs.langmuir.6b00916.
- Engineered Adhesion Peptides for Improved Silicon AdsorptionRamakrishnan SK, Jebors S, Martin M, Cloitre T, Agarwal V, Mehdi A, Martinez J, Subra G, Gergely C. Engineered Adhesion Peptides for Improved Silicon Adsorption. Langmuir 2015, 31: 11868-11874. PMID: 26440047, DOI: 10.1021/acs.langmuir.5b02857.
- Design rules for metal binding biomolecules: understanding of amino acid adsorption on platinum crystallographic facets from density functional calculationsRamakrishnan SK, Martin M, Cloitre T, Firlej L, Gergely C. Design rules for metal binding biomolecules: understanding of amino acid adsorption on platinum crystallographic facets from density functional calculations. Physical Chemistry Chemical Physics 2015, 17: 4193-4198. PMID: 25566851, DOI: 10.1039/c4cp05112b.
- Molecular Mechanism of Selective Binding of Peptides to Silicon SurfaceRamakrishnan SK, Martin M, Cloitre T, Firlej L, Gergely C. Molecular Mechanism of Selective Binding of Peptides to Silicon Surface. Journal Of Chemical Information And Modeling 2014, 54: 2117-2126. PMID: 24936969, DOI: 10.1021/ci500260v.
- Insights on the Facet Specific Adsorption of Amino Acids and Peptides toward PlatinumRamakrishnan SK, Martin M, Cloitre T, Firlej L, Cuisinier F, Gergely C. Insights on the Facet Specific Adsorption of Amino Acids and Peptides toward Platinum. Journal Of Chemical Information And Modeling 2013, 53: 3273-3279. PMID: 24289530, DOI: 10.1021/ci400630d.
- Probing the mechanism of material specific peptides for optical biosensorsRamakrishnan S, Estephan E, Martin M, Cloitre T, Gergely C. Probing the mechanism of material specific peptides for optical biosensors. Proceedings Of SPIE--the International Society For Optical Engineering 2013, 8765: 87650a-87650a-8. DOI: 10.1117/12.2017464.
- Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxelSalehi H, Middendorp E, Panayotov I, Dutilleul P, Vegh AG, Ramakrishnan S, Gergely C, Cuisinier F. Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel. Journal Of Biomedical Optics 2013, 18: 056010-056010. PMID: 23698321, DOI: 10.1117/1.jbo.18.5.056010.
- Detection of apoptosis caused by anticancer drug paclitaxel in MCF-7 cells by confocal Raman microscopySalehi H, Middendorp E, Végh A, Ramakrishnan S, Gergely C, Cuisinier F. Detection of apoptosis caused by anticancer drug paclitaxel in MCF-7 cells by confocal Raman microscopy. Proceedings Of SPIE--the International Society For Optical Engineering 2013, 8594: 85940f-85940f-10. DOI: 10.1117/12.1000149.
- Probing the mechanism of material specific peptides for optical biosensorsRamakrishnan SK, Estephan E, Martin M, Cloitre T, Gergely C, Bio-MEMS and Medical Microdevices, 2013
- Detection of apoptosis caused by anticancer drug paclitaxel in MCF-7 cells by confocal Raman microscopySalehi H, Middendorp E, Panayotov I, Collart Dutilleul PY, Vegh AG, Ramakrishnan S, Gergely C, Cuisinier F., Proceedings Volume 8594, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X, 2013