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Benjamin Turk, PhD

Associate Professor of Pharmacology; Director of Medical Studies, Pharmacology

Contact Information

Benjamin Turk, PhD

Mailing Address

  • Pharmacology

    PO Box 208066, 333 Cedar Street

    New Haven, CT 06520-8066

    United States

Research Summary

The goal of our research is to understand the molecular mechanisms underlying cellular phosphorylation networks, and how these networks are re-wired in cancer cells. The human genome encodes over 500 protein kinases, and mass spectrometry based phosphoproteomics efforts have now cataloged over 100,000 sites of phosphorylation in mammalian cells. These studies have outpaced our ability to understand signaling networks through analysis of individual kinases and their substrates: for the vast majority of the phosphorylation sites, the responsible kinase and functional significance are simply not known. My group studies basic mechanisms used by kinases to target specific substrates within the cell, with the idea of applying this knowledge to identify new kinase-substrate pairs on a proteomic scale. Kinases interact with their substrates through short sequence motifs found both at the site of phosphorylation and at distal sites. We have recently conducted a biochemical screen to identify phosphorylation site motifs recognized by the entire set of human kinases. Information from these screens is used to map cellular phosphorylation networks and to relate mechanisms of substrate targeting to specific structural features of kinases. As an extension of these studies, we are also examining how cancer-associated kinase mutations and treatment with kinase inhibitor drugs lead to "re-wiring" of phosphorylation networks. We are also developing new methodology to identify short linear motifs (SLiMs) in substrates and other interactors that bind to grooves and pockets outside of the kinase catalytic cleft. One outcome of this work is the identification of new strategies and agents for pharmacological targeting of signaling pathways relevant to disease.

Specialized Terms: Cell signaling; Protein kinases; Chemical biology; Proteomics; Protein engineering; Cancer signaling networks


Research Interests

Pharmacology; Protein Kinases; Protein Engineering; Peptide Library; Proteomics; Molecular Mechanisms of Pharmacological Action; Carcinogenesis

Selected Publications