2009
Human hepatic CYP2B6 developmental expression: The impact of age and genotype
Croom E, Stevens J, Hines R, Wallace A, Hodgson E. Human hepatic CYP2B6 developmental expression: The impact of age and genotype. Biochemical Pharmacology 2009, 78: 184-190. PMID: 19464434, DOI: 10.1016/j.bcp.2009.03.029.Peer-Reviewed Original ResearchConceptsYears of ageMedian ageCYP2B6 expressionCYP2B6 levelsProtein levelsSemi-quantitative Western blottingImpact of ageWeeks' gestationNeonatal periodCYP2B6 protein levelsDonor ageCYP3A7 activityPostnatal dayLiver bankAge groupsFetal samplesAdult levelsPediatric samplesWestern blottingCYP2B6 proteinToxicant metabolismAgeSignificant inductionPercentage of samplesPossible role
2008
Novel CYP2C9 Promoter Variants and Assessment of Their Impact on Gene Expression
Kramer M, Rettie A, Rieder M, Cabacungan E, Hines R. Novel CYP2C9 Promoter Variants and Assessment of Their Impact on Gene Expression. Molecular Pharmacology 2008, 73: 1751-1760. PMID: 18310303, PMCID: PMC2413059, DOI: 10.1124/mol.107.044149.Peer-Reviewed Original Research
2007
Ontogeny of human hepatic cytochromes P450
Hines R. Ontogeny of human hepatic cytochromes P450. Journal Of Biochemical And Molecular Toxicology 2007, 21: 169-175. PMID: 17936930, DOI: 10.1002/jbt.20179.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAryl Hydrocarbon HydroxylasesChildChild, PreschoolCytochrome P-450 CYP2C19Cytochrome P-450 CYP2C9Cytochrome P-450 CYP2E1Cytochrome P-450 CYP3ACytochrome P-450 Enzyme SystemGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicHumansInfantLiverMixed Function OxygenasesConceptsHuman hepatic cytochrome P450Adverse drug reactionsConsiderable interindividual variabilityHepatic cytochrome P450Cytochrome P450Human liver samplesImportant endogenous functionsWeeks' gestationThird trimesterFirst trimesterDrug reactionsKey cytochrome P450Postnatal onsetPostnatal increaseTherapeutic efficacyInterindividual variabilityQuantitative Western blottingWestern blottingGestationLow levelsLiver samplesProbe substratesOnset of expressionTrimesterThird group
2005
MOLECULAR MECHANISMS REGULATING HUMAN CYP4B1 LUNG-SELECTIVE EXPRESSION
Poch M, Cutler N, Yost G, Hines R. MOLECULAR MECHANISMS REGULATING HUMAN CYP4B1 LUNG-SELECTIVE EXPRESSION. Drug Metabolism And Disposition 2005, 33: 1174-1184. PMID: 15900016, DOI: 10.1124/dmd.105.004523.Peer-Reviewed Original ResearchConceptsDistal regulatory elementsRegulatory elementsTransient expressionCompetitive electrophoretic mobility shift assaysDNA/protein binding assaysKrüppel-like factor (KLF) familyElectrophoretic mobility shift assaysTranscription factor recognition sequenceChromatin immunoprecipitation assaysTranscription factor sitesProximal elementMobility shift assaysHepG2 hepatoblastoma cellsProtein binding assaysSp1 bindingSp1 elementImmunoprecipitation assaysNuclear proteinsKidney-derived cellsFactor familyShift assaysFactor sitesMutagenesis studiesRegulatory mechanismsMolecular mechanisms
2003
Developmental Expression of Human Hepatic CYP2C9 and CYP2C19
Koukouritaki S, Manro J, Marsh S, Stevens J, Rettie A, McCarver D, Hines R. Developmental Expression of Human Hepatic CYP2C9 and CYP2C19. Journal Of Pharmacology And Experimental Therapeutics 2003, 308: 965-974. PMID: 14634042, DOI: 10.1124/jpet.103.060137.Peer-Reviewed Original ResearchDevelopmental Expression of the Major Human Hepatic CYP3A Enzymes
Stevens J, Hines R, Gu C, Koukouritaki S, Manro J, Tandler P, Zaya M. Developmental Expression of the Major Human Hepatic CYP3A Enzymes. Journal Of Pharmacology And Experimental Therapeutics 2003, 307: 573-582. PMID: 12975492, DOI: 10.1124/jpet.103.054841.Peer-Reviewed Original ResearchCYP2C9exon 4 mutations and warfarin dose phenotype in Asians
Rettie A, Tai G, Veenstra D, Farin F, Srinouanprachan S, Lin Y, Thummel K, Hines R. CYP2C9exon 4 mutations and warfarin dose phenotype in Asians. Blood 2003, 101: 2896-2897. PMID: 12642346, DOI: 10.1182/blood-2002-11-3452.Peer-Reviewed Original ResearchAryl Hydrocarbon HydroxylasesAsian PeopleCytochrome P-450 CYP2C9Dose-Response Relationship, DrugExonsHumansMutationPhenotypeWarfarinGenotyping and site-directed mutagenesis of a cytochrome P450 meander Pro-X-Arg motif critical to CYP4B1 catalysis
Zheng Y, Henne K, Charmley P, Kim R, McCarver D, Cabacungan E, Hines R, Rettie A. Genotyping and site-directed mutagenesis of a cytochrome P450 meander Pro-X-Arg motif critical to CYP4B1 catalysis. Toxicology And Applied Pharmacology 2003, 186: 119-126. PMID: 12639503, DOI: 10.1016/s0041-008x(02)00028-5.Peer-Reviewed Original ResearchConceptsHeme bindingArg motifAmino acid sequence alignmentNonhuman primate speciesPrimate speciesRedox partner interactionsSite-directed mutagenesisCYP4B1 geneRestriction enzyme fragment length polymorphismArg triadFragment length polymorphismRabbit CYP4B1Sequence alignmentHuman allelesN-terminalCatalytic functionCommon laboratory animalsAmino acidsPro residuesLength polymorphismMeander regionHuman populationDistal ArgGenesGlu-Arg
1999
Flavin‐containing monooxygenase isoform 2: Developmental expression in fetal and neonatal rabbit lung
Larsen‐Su S, Krueger S, Yueh M, Lee M, Shehin S, Hines R, Williams D. Flavin‐containing monooxygenase isoform 2: Developmental expression in fetal and neonatal rabbit lung. Journal Of Biochemical And Molecular Toxicology 1999, 13: 187-193. PMID: 10098904, DOI: 10.1002/(sici)1099-0461(1999)13:3/4<187::aid-jbt9>3.0.co;2-6.Peer-Reviewed Original ResearchConceptsEarly developmental appearanceRabbit lungsNeonatal rabbit lungConstitutive cytochrome P450Developmental appearanceNeonatal lungToxic insultsLungMonooxygenase isoformsNumerous xenobioticsForeign chemicalsMonooxygenase functionCytochrome P450Major isoformsTissue-specific fashionDevelopmental expressionMammalian flavinIsoform 2Expression patternsIsoformsFMO2Individual isoformsNeonatesFetuses
1991
Differences in 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin‐inducible CYP1A1 expression in human breast carcinoma cell lines involve altered trans‐acting factors
THOMSEN J, NISSEN L, STACEY S, HINES R, AUTRUP H. Differences in 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin‐inducible CYP1A1 expression in human breast carcinoma cell lines involve altered trans‐acting factors. The FEBS Journal 1991, 197: 577-582. PMID: 2029891, DOI: 10.1111/j.1432-1033.1991.tb15946.x.Peer-Reviewed Original Research
1990
Immunochemical and molecular biological studies on human placental cigarette smoke-inducible cytochrome P-450-dependent monooxygenase activities
Pasanen M, Haaparanta T, Sundin M, Sivonen P, Vakakangas K, Raunio H, Hines R, Gustafsson J, Pelkonen O. Immunochemical and molecular biological studies on human placental cigarette smoke-inducible cytochrome P-450-dependent monooxygenase activities. Toxicology 1990, 62: 175-187. PMID: 1693794, DOI: 10.1016/0300-483x(90)90108-s.Peer-Reviewed Original ResearchMeSH Keywords7-Alkoxycoumarin O-DealkylaseAryl Hydrocarbon HydroxylasesBlotting, NorthernCytochrome P-450 CYP1A1Cytochrome P-450 Enzyme InhibitorsCytochrome P-450 Enzyme SystemEnzyme InductionFemaleGene Expression Regulation, EnzymologicHumansImmunoblottingImmunodiffusionMicrosomesNADPH-Ferrihemoprotein ReductaseOxidoreductasesOxygenasesPlacentaPregnancyRNASmokingConceptsMaternal cigarette smokingAryl hydrocarbon hydroxylaseHuman placental microsomesSmoking mothersCigarette smokingPlacental microsomesHuman placentaMonoclonal antibodiesO-deethylase activityMaternal smokingMonooxygenase activitySmoking statusSignificant inhibitory effectPlacental tissueMAb 2ERDE activitySmokingMAb 1Inhibitory effectLiver NADPH-cytochrome PO-deethylaseECDE activitiesPlacentaCYPIA1 geneAntibodies
1987
Interaction of the 4 S polycyclic aromatic hydrocarbon-binding protein with the cytochrome P-450c gene
Houser W, Cunningham C, Hines R, Schaeffer W, Bresnick E. Interaction of the 4 S polycyclic aromatic hydrocarbon-binding protein with the cytochrome P-450c gene. Archives Of Biochemistry And Biophysics 1987, 259: 215-223. PMID: 2825598, DOI: 10.1016/0003-9861(87)90488-7.Peer-Reviewed Original ResearchAflatoxin B1-4-hydroxylase is associated with cytochrome P3-450 in C57BL6 mouse liver
Koser P, Faletto M, Bansal S, Caballes L, Bresnick E, Hines R, Gurtoo H. Aflatoxin B1-4-hydroxylase is associated with cytochrome P3-450 in C57BL6 mouse liver. Biochemical And Biophysical Research Communications 1987, 142: 872-878. PMID: 3103616, DOI: 10.1016/0006-291x(87)91494-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAroclorsAryl Hydrocarbon HydroxylasesBenzoflavonesBeta-NaphthoflavoneChlorodiphenyl (54% Chlorine)Cytochrome P-450 Enzyme SystemDNAEnzyme InductionFemaleLiverMethylcholanthreneMiceMice, Inbred C57BLMixed Function OxygenasesNucleic Acid HybridizationPolychlorinated BiphenylsRNA, Messenger