2024
Hypermethylated TAGMe as a universal-cancer-only methylation marker and its application in diagnosis and recurrence monitoring of urothelial carcinoma
Yang Z, Chen Q, Dong S, Xu P, Zheng W, Mao Z, Qian C, Zheng X, Dai L, Wang C, Shi H, Li J, Yuan J, Yu W, Xu C. Hypermethylated TAGMe as a universal-cancer-only methylation marker and its application in diagnosis and recurrence monitoring of urothelial carcinoma. Journal Of Translational Medicine 2024, 22: 608. PMID: 38956589, PMCID: PMC11218302, DOI: 10.1186/s12967-024-05420-3.Peer-Reviewed Original ResearchConceptsRecurrence monitoringMethodsThe methylation levelsIndicator of recurrenceBackgroundUrothelial carcinomaClinical recurrenceUrothelial carcinomaBenign diseaseUrological malignanciesPostoperative surveillanceUC diagnosisUC patientsTraining cohortUrothelial markersValidation cohortRecurrenceClinical scenariosUrinary systemClinical utilityCarcinomaMethylation markersPatientsUrine samplesTissue samplesUrineDiagnosismiR‐1246 promotes osteosarcoma cell migration via NamiRNA‐enhancer network dependent on Argonaute 2
Yang S, Zou Q, Liang Y, Zhang D, Peng L, Li W, Li W, Liu M, Tong Y, Chen L, Xu P, Yang Z, Zhou K, Xiao J, Wang H, Yu W. miR‐1246 promotes osteosarcoma cell migration via NamiRNA‐enhancer network dependent on Argonaute 2. MedComm 2024, 5: e543. PMID: 38585233, PMCID: PMC10999177, DOI: 10.1002/mco2.543.Peer-Reviewed Original ResearchArgonaute 2Gene activationMiR-1246Gene expressionCell migrationActivate gene expressionNuclear activating miRNAsActivate gene transcriptionOsteosarcoma cellsOsteosarcoma metastasisOsteosarcoma cell migrationMNNG cellsPAZ domainEnhancer DNAH3K27ac enrichmentMiR-1246 levelActive miRNAsGene transcriptionRNase HMalignant osteosarcoma cellsIn vitro assaysPromote tumor metastasisNamiRNAsArgonauteExtracellular vesiclesPCDHGB7 hypermethylation-based Cervical cancer Methylation (CerMe) detection for the triage of high-risk human papillomavirus-positive women: a prospective cohort study
Cao D, Yang Z, Dong S, Li Y, Mao Z, Lu Q, Xu P, Shao M, Pan L, Han X, Yuan J, Fan Q, Chen L, Wang Y, Zhu W, Yu W, Wang Y. PCDHGB7 hypermethylation-based Cervical cancer Methylation (CerMe) detection for the triage of high-risk human papillomavirus-positive women: a prospective cohort study. BMC Medicine 2024, 22: 55. PMID: 38317152, PMCID: PMC10845746, DOI: 10.1186/s12916-024-03267-5.Peer-Reviewed Original ResearchConceptsHigh-risk human papillomavirusHrHPV-positive womenHigh-risk human papillomavirus-positive womenCervical intraepithelial neoplasia grade 1Triage of hrHPV-positive womenCervical intraepithelial neoplasia grade 2Triage strategiesHuman papillomavirus-positive womenInternational Peace Maternity and Child Health HospitalMaternal and Child Health HospitalMortality of cervical cancerChild Health HospitalProspective cohort studyASC-USLSIL cytologyColposcopy referralHealth HospitalCytology testHuman papillomavirusOver-referralCervical cancerCohort studyMethodsA totalGrade 2Grade 1
2023
Pre-enriched saline gargle samples for detection of SARS-CoV-2
Xu P, Chen J, Qian C, Yu W. Pre-enriched saline gargle samples for detection of SARS-CoV-2. BioScience Trends 2023, 17: 81-84. PMID: 36653152, DOI: 10.5582/bst.2022.01530.Peer-Reviewed Original Research
2022
Mutually exclusive epigenetic modification on SIX6 with hypermethylation for precancerous stage and metastasis emergence tracing
Dong S, Yang Z, Xu P, Zheng W, Zhang B, Fu F, Mao Z, Yuan J, Chen H, Yu W. Mutually exclusive epigenetic modification on SIX6 with hypermethylation for precancerous stage and metastasis emergence tracing. Signal Transduction And Targeted Therapy 2022, 7: 208. PMID: 35790732, PMCID: PMC9256699, DOI: 10.1038/s41392-022-01026-7.Peer-Reviewed Original ResearchHymecromone: a clinical prescription hyaluronan inhibitor for efficiently blocking COVID-19 progression
Yang S, Ling Y, Zhao F, Li W, Song Z, Wang L, Li Q, Liu M, Tong Y, Chen L, Ru D, Zhang T, Zhou K, Zhang B, Xu P, Yang Z, Li W, Song Y, Xu J, Zhu T, Shan F, Yu W, Lu H. Hymecromone: a clinical prescription hyaluronan inhibitor for efficiently blocking COVID-19 progression. Signal Transduction And Targeted Therapy 2022, 7: 91. PMID: 35304437, PMCID: PMC8931182, DOI: 10.1038/s41392-022-00952-w.Peer-Reviewed Original ResearchConceptsLesion absorptionCOVID-19 progressionTreat biliary spasmGround-glass opacitiesControl groupC-reactive proteinInhibitor of HA synthesisHyaluronic acidClinical COVID-19Levels of hyaluronanBiliary spasmClinical parametersD-dimerPulmonary lesionsTherapeutic effectPatientsSARS-CoV-2LymphocytesDrugCOVID-19HymecromoneHuman identical sequencesInhibitorsPrescription drugsHA synthesisSARS-CoV-2 RNA elements share human sequence identity and upregulate hyaluronan via NamiRNA-enhancer network
Li W, Yang S, Xu P, Zhang D, Tong Y, Chen L, Jia B, Li A, Lian C, Ru D, Zhang B, Liu M, Chen C, Fu W, Yuan S, Gu C, Wang L, Li W, Liang Y, Yang Z, Ren X, Wang S, Zhang X, Song Y, Xie Y, Lu H, Xu J, Wang H, Yu W. SARS-CoV-2 RNA elements share human sequence identity and upregulate hyaluronan via NamiRNA-enhancer network. EBioMedicine 2022, 76: 103861. PMID: 35124429, PMCID: PMC8811534, DOI: 10.1016/j.ebiom.2022.103861.Peer-Reviewed Original ResearchConceptsHuman identical sequencesRNA elementsHuman genomeSARS-CoV-2 genomeHyaluronan synthase 2Chromatin immunoprecipitation assaysDistant genesSequence similarityGenomic fragmentsSequence identityTarget lociGenomic interactionsMiRNA precursorsH3K27ac enrichmentSARS-CoV-2Identical sequencesBioinformatics analysisImmunoprecipitation assaysGenomeGene expressionHEK293TQuantitative RT-PCRShort sequencesGenesHost enhancementHypermethylated PCDHGB7 as a Biomarker for Early Detection of Endometrial Cancer in Endometrial Brush Samples and Cervical Scrapings
Yuan J, Mao Z, Lu Q, Xu P, Wang C, Xu X, Zhou Z, Zhang T, Yu W, Dong S, Wang Y, Cheng W. Hypermethylated PCDHGB7 as a Biomarker for Early Detection of Endometrial Cancer in Endometrial Brush Samples and Cervical Scrapings. Frontiers In Molecular Biosciences 2022, 8: 774215. PMID: 35059435, PMCID: PMC8763697, DOI: 10.3389/fmolb.2021.774215.Peer-Reviewed Original ResearchEndometrial brush samplingEndometrial cancerCervical scrapesBrush samplesMethylation-sensitive restriction enzymesMethylation markersEndometrial tissueNovel methylation markersGene Expression Omnibus (GEODNA methylation markersEarly detection of endometrial cancerDetection of endometrial cancerEarly detection of ECPredictive valueTao Brush samplingMinimally invasive detection methodRestriction enzymesMethylation dataCancer Genome AtlasNegative predictive valueMethylation detectionEndometrial cancer detectionPositive predictive valueEarly detectionMSRE-qPCR
2021
Reactivation of tumour suppressor in breast cancer by enhancer switching through NamiRNA network
Liang Y, Lu Q, Li W, Zhang D, Zhang F, Zou Q, Chen L, Tong Y, Liu M, Wang S, Li W, Ren X, Xu P, Yang Z, Dong S, Zhang B, Huang Y, Li D, Wang H, Yu W. Reactivation of tumour suppressor in breast cancer by enhancer switching through NamiRNA network. Nucleic Acids Research 2021, 49: 8556-8572. PMID: 34329471, PMCID: PMC8421228, DOI: 10.1093/nar/gkab626.Peer-Reviewed Original ResearchMeSH KeywordsBreast NeoplasmsCarcinogenesisDNA MethylationEnhancer Elements, GeneticEpigenomicsFemaleGene Expression Regulation, NeoplasticGene SilencingHumansMicroRNAsPromoter Regions, GeneticReceptors, EstrogenReceptors, G-Protein-CoupledRegulatory Sequences, Nucleic AcidRNA, NeoplasmTumor Suppressor ProteinsConceptsTumor suppressor geneLowest expression of miRNABreast cancerLow expressionDysfunction of tumor suppressor genesLoss of function of tumor suppressor genesTriple negative breast cancer subtypeCancer developmentFunction of tumor suppressor genesMiR-339Inactivation of tumor suppressor genesClinical breast cancer treatmentTumor suppressor gene inactivationExpression of miRNAsReactivation of tumor suppressor genesBreast cancer subtypesBreast cancer cellsBreast cancer treatmentLuminal A/BPaired breast cancerAberrant histone modificationsGPER1 expressionEpigenetic abnormalitiesCancer subtypesSuppressor geneUHRF1 regulates alternative splicing by interacting with splicing factors and U snRNAs in a H3R2me involved manner
Xu P, Zhang L, Xiao Y, Li W, Hu Z, Zhang R, Li J, Wu F, Xi Y, Zou Q, Wang Z, Guo R, Ma H, Dong S, Xiao M, Yang Z, Ren X, Wei C, Yu W. UHRF1 regulates alternative splicing by interacting with splicing factors and U snRNAs in a H3R2me involved manner. Human Molecular Genetics 2021, 30: 2110-2122. PMID: 34196368, DOI: 10.1093/hmg/ddab178.Peer-Reviewed Original ResearchConceptsU snRNAsSplicing factorsDNA methylation maintenanceAlternative splicing eventsFunctions of UHRF1RNA splicing regulationAlternative RNA splicingDNA damage repairU2 snRNAMethylation maintenanceProtein interactomeRNA metabolismSplicing regulationRNA splicingSplicing eventsSpliceosome componentsAlternative splicingRNA interactomeUHRF1Chromatin configurationBinding preferencesCell cycleMolecular basisBiological processesMethylation statusHypermethylated PCDHGB7 as a universal cancer only marker and its application in early cervical cancer screening
Dong S, Lu Q, Xu P, Chen L, Duan X, Mao Z, Zhang B, Sui L, Wang Y, Yu W. Hypermethylated PCDHGB7 as a universal cancer only marker and its application in early cervical cancer screening. Clinical And Translational Medicine 2021, 11: e457. PMID: 34185407, PMCID: PMC8214855, DOI: 10.1002/ctm2.457.Peer-Reviewed Original Research
2020
An electroosmotic flow-free two-direction migration strategy enables fast affinity capillary electrophoresis to study the weak interactions between basic peptides and RNA
Yuan Z, Xu P, Yu F, Zhang D, Zhao Q, Yu W, Wang H. An electroosmotic flow-free two-direction migration strategy enables fast affinity capillary electrophoresis to study the weak interactions between basic peptides and RNA. Analytical Methods 2020, 12: 5833-5838. PMID: 33227112, DOI: 10.1039/d0ay01515f.Peer-Reviewed Original ResearchConceptsAffinity capillary electrophoresisCapillary electrophoresisWeak interactionsAffinity capillary electrophoresis methodHistone peptidesStudy noncovalent interactionsPeptide-RNA interactionsBinding complexPeptide-RNA complexesNoncovalent interactionsU2 RNACE migrationNeutral capillaryMethylation stateFast dissociationFree probeMigration strategyElectroosmotic flowRNAElectrophoresisMigration distancePeptideCE approachComplexElectric field
2019
Histone-Related Genes Are Hypermethylated in Lung Cancer and Hypermethylated HIST1H4F Could Serve as a Pan-Cancer Biomarker
Dong S, Li W, Wang L, Hu J, Song Y, Zhang B, Ren X, Ji S, Li J, Xu P, Liang Y, Chen G, Lou J, Yu W. Histone-Related Genes Are Hypermethylated in Lung Cancer and Hypermethylated HIST1H4F Could Serve as a Pan-Cancer Biomarker. Cancer Research 2019, 79: 6101-6112. PMID: 31575549, DOI: 10.1158/0008-5472.can-19-1019.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overBiomarkers, TumorBronchoalveolar Lavage FluidCell Line, TumorCpG IslandsDatasets as TopicDNA MethylationEarly Detection of CancerFemaleGene Expression Regulation, NeoplasticGenetic LociHistonesHumansLung NeoplasmsMaleMiddle AgedPromoter Regions, GeneticWhole Genome SequencingConceptsHistone-related genesWhole-genome DNA methylation analysisDNA methylation analysisHistone genesCancer Genome AtlasMethylation signaturesMethylation analysisGenesGenome AtlasPan-cancerBronchoalveolar lavage fluid samplesHypermethylationLavage fluid samplesEarly cancer diagnosisTCGA datasetPan-cancer biomarkerLung cancerTCGACancer-related deathsLung cancer cohortMethylationCancer cohortTumor typesTumorigenesisCancer diagnosisGuide Positioning Sequencing identifies aberrant DNA methylation patterns that alter cell identity and tumor-immune surveillance networks
Li J, Li Y, Li W, Luo H, Xi Y, Dong S, Gao M, Xu P, Zhang B, Liang Y, Zou Q, Hu X, Peng L, Zou D, Wang T, Yang H, Jiang C, Peng S, Wu F, Yu W. Guide Positioning Sequencing identifies aberrant DNA methylation patterns that alter cell identity and tumor-immune surveillance networks. Genome Research 2019, 29: 270-280. PMID: 30670627, PMCID: PMC6360814, DOI: 10.1101/gr.240606.118.Peer-Reviewed Original ResearchConceptsWhole-genome bisulfite sequencingAberrant DNA methylationDNA methylationCell identityPredictor of gene expressionGenome-wide DNA methylationWhole-genome DNA methylationAberrant DNA methylation patternsDNA methylation patternsExpression of genes associated with immunityGenes associated with immunityAltering cell identityGene bodiesGC-richGenomic variationRepetitive regionsBisulfite sequencingMethylation patternsMethylation differencesGene expressionModulate expressionImmune surveillance networkLiver cancer developmentSequenceCancer development
2018
An epigenetic perspective on tumorigenesis: Loss of cell identity, enhancer switching, and NamiRNA network
Liang Y, Xu P, Zou Q, Luo H, Yu W. An epigenetic perspective on tumorigenesis: Loss of cell identity, enhancer switching, and NamiRNA network. Seminars In Cancer Biology 2018, 57: 1-9. PMID: 30213688, DOI: 10.1016/j.semcancer.2018.09.001.Peer-Reviewed Original ResearchAn epigenetic perspective on tumorigenesis: Loss of cell identity, enhancer switching, and NamiRNA network
Liang Y, Xu P, Zou Q, Luo H, Yu W. An epigenetic perspective on tumorigenesis: Loss of cell identity, enhancer switching, and NamiRNA network. Seminars In Cancer Biology 2018, 83: 596-604. PMID: 30208341, DOI: 10.1016/j.semcancer.2018.09.004.Peer-Reviewed Original Research