2015
Human and murine erythropoiesis
An X, Schulz VP, Mohandas N, Gallagher PG. Human and murine erythropoiesis. Current Opinion In Hematology 2015, 22: 206-211. PMID: 25719574, PMCID: PMC4401149, DOI: 10.1097/moh.0000000000000134.Peer-Reviewed Original ResearchConceptsTerminal erythroid differentiationMurine erythropoiesisPerturbed erythropoiesisErythroid differentiationStage-specific programsAlternative splicing programGenome-wide analysisPoor sequence conservationSpecies-specific similaritiesGene expression dataGood model systemSplicing programGenomic methodologiesSequence conservationTranscriptome analysisHuman erythropoiesisExpression dataDifferentiation stageRecent insightsModel systemErythropoiesisDifferentiationFundamental mechanismsCritical insightsDifferent mechanisms
2014
Isolation and transcriptome analyses of human erythroid progenitors: BFU-E and CFU-E
Li J, Hale J, Bhagia P, Xue F, Chen L, Jaffray J, Yan H, Lane J, Gallagher PG, Mohandas N, Liu J, An X. Isolation and transcriptome analyses of human erythroid progenitors: BFU-E and CFU-E. Blood 2014, 124: 3636-3645. PMID: 25339359, PMCID: PMC4256913, DOI: 10.1182/blood-2014-07-588806.Peer-Reviewed Original ResearchConceptsHuman BFUProgenitor populationsErythroid progenitorsDistinct progenitor populationsColony-forming unit-erythroid (CFU-E) cellsCFU-E cellsCFU-E coloniesCFU-E progenitorsHuman erythroid progenitorsUnit erythroid cellsColony assayErythroid cell culturesErythroid progenitor populationsTranscriptome analysisUnique transcriptomeStem cell factorCell culturesBioinformatics analysisHuman erythropoiesisFlow cytometry-based strategyMolecular characterizationBFU-E coloniesDifferentiation stagePrimary cellsCell factorGlobal transcriptome analyses of human and murine terminal erythroid differentiation
An X, Schulz VP, Li J, Wu K, Liu J, Xue F, Hu J, Mohandas N, Gallagher PG. Global transcriptome analyses of human and murine terminal erythroid differentiation. Blood 2014, 123: 3466-3477. PMID: 24637361, PMCID: PMC4041167, DOI: 10.1182/blood-2014-01-548305.Peer-Reviewed Original ResearchConceptsTerminal erythroid differentiationErythroid differentiationGene expressionMurine terminal erythroid differentiationStage-specific transcriptomesDifferentiation stageGlobal transcriptome analysisStage-specific patternsRNA sequencing analysisGene expression profilesDistinct developmental stagesMurine transcriptomesFluorescence-activated cell sortingTranscriptional spaceErythroid developmentMurine erythroblastsTranscriptome analysisUnique transcriptomeBioinformatics analysisPerturbed erythropoiesisTranscriptomeExpression profilesErythroid disordersDevelopmental stagesSequencing analysis