Michael Chiorazzi, MD, PhD
Cards
About
Research
Publications
2023
Autologous humanized PDX modeling for immuno-oncology recapitulates features of the human tumor microenvironment
Chiorazzi M, Martinek J, Krasnick B, Zheng Y, Robbins K, Qu R, Kaufmann G, Skidmore Z, Juric M, Henze L, Brösecke F, Adonyi A, Zhao J, Shan L, Sefik E, Mudd J, Bi Y, Goedegebuure S, Griffith M, Griffith O, Oyedeji A, Fertuzinhos S, Garcia-Milian R, Boffa D, Detterbeck F, Dhanasopon A, Blasberg J, Judson B, Gettinger S, Politi K, Kluger Y, Palucka K, Fields R, Flavell R. Autologous humanized PDX modeling for immuno-oncology recapitulates features of the human tumor microenvironment. Journal For ImmunoTherapy Of Cancer 2023, 11: e006921. PMID: 37487666, PMCID: PMC10373695, DOI: 10.1136/jitc-2023-006921.Peer-Reviewed Original ResearchConceptsHuman tumor microenvironmentTumor microenvironmentTumor-immune interactionsSolid tumorsAdaptive immune activationAdaptive immune populationsIndividual tumor microenvironmentsPatient's hematopoietic systemPatient-derived xenograft tissuesVascular endothelial growth factorBone marrow hematopoietic stemBone marrow aspiratePreclinical drug testingEndothelial growth factorHematopoietic systemAutologous tumorPDX modelingPDX miceImmune activationImmune populationsMarrow aspiratesAutologous systemIndividual patientsLittermate controlsPreclinical predictions
2022
Platelet-derived TLT-1 promotes tumor progression by suppressing CD8+ T cells
Tyagi T, Jain K, Yarovinsky TO, Chiorazzi M, Du J, Castro C, Griffin J, Korde A, Martin KA, Takyar SS, Flavell RA, Patel AA, Hwa J. Platelet-derived TLT-1 promotes tumor progression by suppressing CD8+ T cells. Journal Of Experimental Medicine 2022, 220: e20212218. PMID: 36305874, PMCID: PMC9814191, DOI: 10.1084/jem.20212218.Peer-Reviewed Original ResearchConceptsCD8 T cellsT cellsTLT-1Non-small cell lung cancer patientsCell lung cancer patientsTREM-like transcript-1Tumor immunosuppressive mechanismsT cell suppressionLung cancer patientsPatient T cellsNF-κB pathwayPatient-derived tumorsDistinct activation phenotypesNSCLC patientsImmunosuppressive mechanismsSyngeneic tumorsHumanized miceImmunoregulatory rolePrognostic significanceImmunocompetent miceCancer patientsCell suppressionActivation phenotypeReduced tumorTumor growthTranscriptional profiling of macrophages in situ in metastatic melanoma reveals localization-dependent phenotypes and function
Martinek J, Lin J, Kim KI, Wang VG, Wu TC, Chiorazzi M, Boruchov H, Gulati A, Seeniraj S, Sun L, Marches F, Robson P, Rongvaux A, Flavell RA, George J, Chuang JH, Banchereau J, Palucka K. Transcriptional profiling of macrophages in situ in metastatic melanoma reveals localization-dependent phenotypes and function. Cell Reports Medicine 2022, 3: 100621. PMID: 35584631, PMCID: PMC9133468, DOI: 10.1016/j.xcrm.2022.100621.Peer-Reviewed Original ResearchConceptsMonocytes/macrophagesDendritic cellsMetastatic melanomaMelanoma cellsMetastatic cutaneous melanomaRegulation of toleranceSpecific transcriptional signatureTranscriptional profilingGene setsLaser capture microdissectionCell-based therapiesTranscriptional signatureCutaneous melanomaDC functionPatient outcomesMetastatic cancerTumor nestsTCGA cohortImmune functionCandidate biomarkersCD14Stromal macrophagesTumor siteTherapeutic opportunitiesAntigen capture
2017
No Oxygen? No Glucose? No Problem: Fatty Acid Catabolism Enhances Effector CD8+ TILs
Bailis W, Shyer JA, Chiorazzi M, Flavell RA. No Oxygen? No Glucose? No Problem: Fatty Acid Catabolism Enhances Effector CD8+ TILs. Cancer Cell 2017, 32: 280-281. PMID: 28898693, PMCID: PMC6939383, DOI: 10.1016/j.ccell.2017.08.013.Peer-Reviewed Original Research
2013
Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma
Chiorazzi M, Rui L, Yang Y, Ceribelli M, Tishbi N, Maurer CW, Ranuncolo SM, Zhao H, Xu W, Chan WC, Jaffe ES, Gascoyne RD, Campo E, Rosenwald A, Ott G, Delabie J, Rimsza LM, Shaham S, Staudt LM. Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 3943-3948. PMID: 23431138, PMCID: PMC3593917, DOI: 10.1073/pnas.1217271110.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsApoptosisBase SequenceCaenorhabditis elegansCaenorhabditis elegans ProteinsCaspasesCell Line, TumorEnzyme ActivationF-Box ProteinsHEK293 CellsHumansLymphomaLymphoma, Large B-Cell, DiffuseMolecular Sequence DataMutation, MissenseProto-Oncogene Proteins c-bcl-2Repressor ProteinsRNA, MessengerRNA, NeoplasmSequence Homology, Amino AcidConceptsCED-9Cell deathCaenorhabditis elegansDRE-1Caspase CED-3Control cell deathApoptotic cell deathRegulators of apoptosisEGL-1CED-3Cell fateHuman diffuse large B-cell lymphomaHuman proteinsCaspase activationFBXO10BCL2 proteinElegansProteinHuman malignanciesHuman lymphomasAlternative mechanismApoptosisActivationBH3Regulator
2009
Characterization of early stages of human B cell development by gene expression profiling
Hystad M, Myklebust J, Bø T, Sivertsen E, Rian E, Forfang L, Munthe E, Rosenwald A, Chiorazzi M, Jonassen I, Staudt L, Smeland E. Characterization of early stages of human B cell development by gene expression profiling. The Journal Of Immunology 2009, 182: 5882-5882. DOI: 10.4049/jimmunol.0990031.Peer-Reviewed Original Research
2007
Characterization of Early Stages of Human B Cell Development by Gene Expression Profiling
Hystad ME, Myklebust JH, Bø TH, Sivertsen EA, Rian E, Forfang L, Munthe E, Rosenwald A, Chiorazzi M, Jonassen I, Staudt LM, Smeland EB. Characterization of Early Stages of Human B Cell Development by Gene Expression Profiling. The Journal Of Immunology 2007, 179: 3662-3671. PMID: 17785802, DOI: 10.4049/jimmunol.179.6.3662.Peer-Reviewed Original ResearchConceptsHuman B cell developmentB cell developmentGene expression profilingCell developmentTranscription factorsExpression profilingHemopoietic stem cellsLineage-associated transcription factorsInteresting expression patternsStem cellsMyeloid-associated genesExpression of genesAdult bone marrowFunctional assignmentImmature B cellsKey genesCDNA microarrayGene setsExpression patternsVDJ recombinationUnknown roleB-lymphoid cellsGenesB cellsRNA amplificationTiming of the onset of a developmental cell death is controlled by transcriptional induction of the C. elegans ced-3 caspase-encoding gene
Maurer CW, Chiorazzi M, Shaham S. Timing of the onset of a developmental cell death is controlled by transcriptional induction of the C. elegans ced-3 caspase-encoding gene. Development 2007, 134: 1357-1368. PMID: 17329362, DOI: 10.1242/dev.02818.Peer-Reviewed Original ResearchConceptsCED-3 caspaseCell deathEGL-1Animal developmentTranscriptional inductionCed-9/BclCED-4/ApafTemporal controlPAL-1Such temporal regulationC. elegans homologCell death onsetDevelopmental expression patternsDevelopmental cell deathCell-specific deathEgl-1 expressionCED-4CED-3CED-9Transcriptional regulationSomatic cellsTemporal regulationPromoter sitesExpression patternsTranscriptionPoint mutations and genomic deletions in CCND1 create stable truncated cyclin D1 mRNAs that are associated with increased proliferation rate and shorter survival
Wiestner A, Tehrani M, Chiorazzi M, Wright G, Gibellini F, Nakayama K, Liu H, Rosenwald A, Muller-Hermelink HK, Ott G, Chan WC, Greiner TC, Weisenburger DD, Vose J, Armitage JO, Gascoyne RD, Connors JM, Campo E, Montserrat E, Bosch F, Smeland EB, Kvaloy S, Holte H, Delabie J, Fisher RI, Grogan TM, Miller TP, Wilson WH, Jaffe ES, Staudt LM. Point mutations and genomic deletions in CCND1 create stable truncated cyclin D1 mRNAs that are associated with increased proliferation rate and shorter survival. Blood 2007, 109: 4599-4606. PMID: 17299095, PMCID: PMC1885523, DOI: 10.1182/blood-2006-08-039859.Peer-Reviewed Original Research
2006
Characterization of Early Human B Cell Development by Gene Expression Profiling.
Hystad M, Bo T, Rian E, Myklebust J, Sivertsen E, Forfang L, Chiorazzi M, Rosenwald A, Jonassen I, Staudt L, Smeland E. Characterization of Early Human B Cell Development by Gene Expression Profiling. Blood 2006, 108: 1352. DOI: 10.1182/blood.v108.11.1352.1352.Peer-Reviewed Original ResearchB cell developmentHematopoietic stem cellsHuman B cell developmentGene expression profilingGene expressionFluorescence-activated cell sortingHigh expression levelsImmature B cellsGene OntologyExpression profilingCell developmentB cell lineage commitmentCell populationsTranscription factor E2AEarly B cell developmentExpression levelsExpression of genesProgenitor B cellsPro-B CellsSubsequent fluorescence-activated cell sortingAdult human bone marrowB lineage cellsGene pairsRecombination genesLineage commitment
Clinical Care
Overview
Clinical Specialties
Medical Oncology; Head & Neck Cancers
Board Certifications
Medical Oncology
- Certification Organization
- AB of Internal Medicine
- Original Certification Date
- 2022
Internal Medicine
- Certification Organization
- AB of Internal Medicine
- Original Certification Date
- 2016
Links & Media
News
- April 12, 2024
Yale Cancer Center Faculty and Trainees Present at AACR Annual Meeting
- August 03, 2023Source: YaleNews
Method Allows New Insights Into Interplay Between Immune System and Tumors
- April 10, 2023
Yale Cancer Center Experts to Present New Research at Annual AACR Meeting
- January 11, 2023
Discoveries & Impact (January 2023)