Liana Boraas
Associate Research ScientistCards
About
Research
Publications
2018
Actin and myosin II modulate differentiation of pluripotent stem cells
Boraas L, Pineda E, Ahsan T. Actin and myosin II modulate differentiation of pluripotent stem cells. PLOS ONE 2018, 13: e0195588. PMID: 29664925, PMCID: PMC5903644, DOI: 10.1371/journal.pone.0195588.Peer-Reviewed Original ResearchConceptsPluripotent stem cellsMesodermal lineagesActin polymerizationStem cellsActin-myosin molecular motorEmbryonic stem cellsCell-based therapiesGerm lineageCytoskeletal tensionEndodermal lineagesDifferentiation eventsEmbryoid bodiesLineagesSpontaneous differentiationIntracellular factorsModulates differentiationMolecular motorsEarly differentiationStem cell-based therapiesActin-myosin interactionCytoskeletonDifferentiationRegenerative medicineEndothelial cellsCells
2016
Lack of vimentin impairs endothelial differentiation of embryonic stem cells
Boraas L, Ahsan T. Lack of vimentin impairs endothelial differentiation of embryonic stem cells. Scientific Reports 2016, 6: 30814. PMID: 27480130, PMCID: PMC4969593, DOI: 10.1038/srep30814.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsEmbryoid bodiesKnockout embryonic stem cellsWild-type embryonic stem cellsStem cellsEndothelial phenotypeCell typesWT EBsSpecification pathwayCytoskeletal filament vimentinGene expressionSpontaneous differentiationPluripotency markersEndothelial differentiationProper functionVE-cadherinPresence of vimentinPhenotypeProtein expressionDifferentiationDecreased expressionExpressionEndothelial cellsCellsAdult miceCytoskeletal Expression and Remodeling in Pluripotent Stem Cells
Boraas L, Guidry J, Pineda E, Ahsan T. Cytoskeletal Expression and Remodeling in Pluripotent Stem Cells. PLOS ONE 2016, 11: e0145084. PMID: 26771179, PMCID: PMC4714815, DOI: 10.1371/journal.pone.0145084.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsInduced pluripotent stem cellsPluripotent stem cellsCytoskeletal proteinsStem cellsMouse embryonic stem cellsEmbryoid body modelIPSC samplesUndifferentiated pluripotent stem cellsSimilar gene expressionCell-based therapiesLamin ACytoskeletal networkEmbryonic fibroblastsCytoskeletal intermediate filamentsGene expressionParental phenotypesGene levelSmooth muscle cell actinSpontaneous differentiationCytoskeletal markersCell actinDifferentiation studiesCytoskeletal expressionIntermediate filaments
2013
Three-Dimensional Cell Culture Environment Promotes Partial Recovery of the Native Corneal Keratocyte Phenotype from a Subcultured Population
Thompson R, Boraas L, Sowder M, Bechtel M, Orwin E. Three-Dimensional Cell Culture Environment Promotes Partial Recovery of the Native Corneal Keratocyte Phenotype from a Subcultured Population. Tissue Engineering Part A 2013, 19: 1564-1572. PMID: 23410050, PMCID: PMC3665321, DOI: 10.1089/ten.tea.2012.0084.Peer-Reviewed Original ResearchConceptsAlpha-smooth muscle actinOnly current treatmentFourth leading causePartial recoveryAldehyde dehydrogenase 1A1World Health OrganizationThree-dimensional (3D) cell culture environmentsProinflammatory cytokinesCorneal markersCorneal opacityQuantitative real-time PCRLeading causeCurrent treatmentReal-time PCRCorneal diseaseALDH1A1 expressionRabbit corneal keratocytesTissue transplantsCorneal phenotypeMuscle actinWestern blotPresence of serumActin expressionHealth OrganizationCulture conditions