2019
IL-27 and TCR Stimulation Promote T Cell Expression of Multiple Inhibitory Receptors
DeLong JH, O'Hara Hall A, Rausch M, Moodley D, Perry J, Park J, Phan AT, Beiting DP, Kedl RM, Hill JA, Hunter CA. IL-27 and TCR Stimulation Promote T Cell Expression of Multiple Inhibitory Receptors. ImmunoHorizons 2019, 3: 13-25. PMID: 31356173, PMCID: PMC6994206, DOI: 10.4049/immunohorizons.1800083.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsB7-H1 AntigenCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCostimulatory and Inhibitory T-Cell ReceptorsCTLA-4 AntigenFemaleInterleukinsLymphocyte ActivationMiceMice, Inbred BALB CMice, Inbred C57BLReceptors, Antigen, T-CellReceptors, ImmunologicSpleenToxoplasmaToxoplasmosisTranscriptomeTransfectionConceptsT cell expressionMultiple inhibitory receptorsIL-27Inhibitory receptorsPD-L1LAG-3Cell expressionCTLA-4Effector cell expressionTCR stimulationEffector T cellsIL-27 productionT cell activationTim-3Cell surface moleculesCell-extrinsic factorsT cellsIR expressionCell activationTIGITInfectionTCR signalsSurface moleculesSynergistic inductionVivo overexpression
2018
Cytokine- and TCR-Mediated Regulation of T Cell Expression of Ly6C and Sca-1.
DeLong JH, Hall AO, Konradt C, Coppock GM, Park J, Harms Pritchard G, Hunter CA. Cytokine- and TCR-Mediated Regulation of T Cell Expression of Ly6C and Sca-1. The Journal Of Immunology 2018, 200: 1761-1770. PMID: 29358280, PMCID: PMC5821564, DOI: 10.4049/jimmunol.1701154.Peer-Reviewed Original ResearchConceptsT cell expressionIL-27T cellsEffector cellsSca-1Cell expressionPathogen-specific T cellsMemory precursor effector cellsEndogenous IL-27Identification of CD8Type I IFNMemory stem cellsCytokine-mediated signalsEffector CD4Ly6C expressionSca-1 expressionImmune populationsT-betExpression of Ly6CMemory populationMouse CD4I IFNLy6CIFNMouse splenocytes
2017
IL-27 LIMITS TYPE 2 IMMUNOPATHOLOGY FOLLOWING PARAINFLUENZA VIRUS INFECTION
Muallem G, Wagage S, Sun Y, DeLong JH, Valenzuela A, Christian DA, Pritchard G, Fang Q, Buza EL, Jain D, Elloso MM, López CB, Hunter CA. IL-27 LIMITS TYPE 2 IMMUNOPATHOLOGY FOLLOWING PARAINFLUENZA VIRUS INFECTION. PLOS Pathogens 2017, 13: e1006173. PMID: 28129374, PMCID: PMC5305264, DOI: 10.1371/journal.ppat.1006173.Peer-Reviewed Original ResearchConceptsParainfluenza virus infectionTh2 responsesIL-27Control miceT cellsVirus infectionParamyxovirus infectionDisease severityWeight lossPathogenic Th2 responsesQuality of CD4Type 2 immunopathologyT-cell depletionT cell responsesSevere lung lesionsPopulation of IFNParainfluenza infectionsPulmonary eosinophilsRespiratory paramyxovirusesT helperCell depletionLung lesionsImmune responseLimit pathologyCD4
2014
Grass‐specific CD4+ T‐cells exhibit varying degrees of cross‐reactivity, implications for allergen‐specific immunotherapy
Archila LD, DeLong JH, Wambre E, James EA, Robinson DM, Kwok WW. Grass‐specific CD4+ T‐cells exhibit varying degrees of cross‐reactivity, implications for allergen‐specific immunotherapy. Clinical & Experimental Allergy 2014, 44: 986-998. PMID: 24708411, PMCID: PMC4069242, DOI: 10.1111/cea.12324.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAllergensAmino Acid SequenceAntigens, PlantBasophilsCD4-Positive T-LymphocytesCross ReactionsCytokinesDesensitization, ImmunologicEpitopes, T-LymphocyteHLA AntigensHumansImmunoglobulin ELymphocyte ActivationPeptidesPoaceaePollenT-Cell Antigen Receptor SpecificityT-Lymphocyte SubsetsConceptsT cell epitopesReactive T cellsCross-reactive T-cell epitopesT cell levelsT cellsAllergic subjectsIntracellular cytokine staining assaysCross-reactive profilesEx vivo stainingAllergen-specific immunotherapySpecific T cellsGrass pollen allergensPhl p 1Staining assaysPhl pTetramer stainingImmunotherapy strategiesAllergic responsesComparison of frequencyAntigenic epitopesComparable frequencyVivo stainingEpitopesImmunotherapyAllergens
2013
Specific immunotherapy modifies allergen-specific CD4+ T-cell responses in an epitope-dependent manner
Wambre E, DeLong JH, James EA, Torres-Chinn N, Pfützner W, Möbs C, Durham SR, Till SJ, Robinson D, Kwok WW. Specific immunotherapy modifies allergen-specific CD4+ T-cell responses in an epitope-dependent manner. Journal Of Allergy And Clinical Immunology 2013, 133: 872-879.e7. PMID: 24373351, PMCID: PMC3961577, DOI: 10.1016/j.jaci.2013.10.054.Peer-Reviewed Original ResearchConceptsT cell responsesGrass pollen allergyAllergen-specific immunotherapyPollen allergyAllergen-specific T-cell responsesAllergen-specific Th2 cellsAllergen-specific CD4Epitope-dependent mannerEpitope hierarchyTr1 responsesTr1 cellsNonatopic subjectsAllergic inflammationPeripheral toleranceAllergic subjectsVaccine strategiesTh2 cellsAntigen stimulationDistinct functional profilesT cellsEpitope levelAllergyEpitope changesImmunotherapyCell deletion
2012
Frequency of Epitope-Specific Naive CD4+ T Cells Correlates with Immunodominance in the Human Memory Repertoire
Kwok WW, Tan V, Gillette L, Littell CT, Soltis MA, LaFond RB, Yang J, James EA, DeLong JH. Frequency of Epitope-Specific Naive CD4+ T Cells Correlates with Immunodominance in the Human Memory Repertoire. The Journal Of Immunology 2012, 188: 2537-2544. PMID: 22327072, PMCID: PMC3997369, DOI: 10.4049/jimmunol.1102190.Peer-Reviewed Original ResearchMeSH KeywordsAnthrax VaccinesCD4-Positive T-LymphocytesCell SeparationEpitopes, T-LymphocyteFlow CytometryHLA-DRB1 ChainsHumansImmunodominant EpitopesImmunologic MemoryConceptsT cellsMemory repertoireAg-specific T cellsNaive repertoireNaive precursor frequencyT cell responsesFrequency of precursorsProtective AgImmunized subjectsTCR usageNaive CD4Precursor frequencyEpitope specificityCell responsesMurine repertoireDifferent epitopesNonvaccineesVaccineesImmunodominanceEpitopesCellsHuman repertoireSubjectsCD4HLA
2011
Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy
Wambre E, DeLong JH, James EA, LaFond RE, Robinson D, Kwok WW. Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy. Journal Of Allergy And Clinical Immunology 2011, 129: 544-551.e7. PMID: 21975176, PMCID: PMC3268867, DOI: 10.1016/j.jaci.2011.08.034.Peer-Reviewed Original ResearchConceptsNonallergic subjectsT cellsPollen allergyPeptide-MHC class II tetramersAllergen-specific T cellsChemoattractant receptor-homologous moleculeAllergen-specific CD4Natural pollen exposureAllergen-specific immunotherapyClass II tetramersProtective immune responseT cell outcomesIL-10 inductionLymphocyte expressionSpecific immunotherapyIL-10Allergic subjectsAllergy vaccinationCytokine productionImmune responseDifferentiation stageSeasonal allergiesPollen exposureAllergyProtective responseAra h 1–reactive T cells in individuals with peanut allergy
DeLong JH, Simpson KH, Wambre E, James EA, Robinson D, Kwok WW. Ara h 1–reactive T cells in individuals with peanut allergy. Journal Of Allergy And Clinical Immunology 2011, 127: 1211-1218.e3. PMID: 21459424, PMCID: PMC3130623, DOI: 10.1016/j.jaci.2011.02.028.Peer-Reviewed Original ResearchMeSH KeywordsAdultAmino Acid SequenceAntigens, PlantArachisCD4-Positive T-LymphocytesCytokinesEpitope MappingEpitopes, T-LymphocyteFemaleGlycoproteinsHumansImmunologic MemoryInterleukin-4MaleMembrane ProteinsMiddle AgedMolecular Sequence DataPeanut HypersensitivityPlant ProteinsReceptors, CCR4Young Adult
2010
Direct ex vivo analysis of allergen-specific CD4+ T cells
Kwok WW, Roti M, DeLong JH, Tan V, Wambre E, James EA, Robinson D. Direct ex vivo analysis of allergen-specific CD4+ T cells. Journal Of Allergy And Clinical Immunology 2010, 125: 1407-1409.e1. PMID: 20513526, PMCID: PMC3909500, DOI: 10.1016/j.jaci.2010.03.037.Peer-Reviewed Original Research