2019
FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia
Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O’Toole E, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Molecular Biology Of The Cell 2019, 30: 2659-2680. PMID: 31483737, PMCID: PMC6761771, DOI: 10.1091/mbc.e19-07-0367.Peer-Reviewed Original ResearchConceptsInner dynein armsRegulatory complexCryo-electron tomographyInner arm dyneinsCiliary motilityMultiple dynein motorsPrecise spatial organizationAxonemal repeatDocking factorUnique binding siteWD repeatsDynein assemblyAssembly factorsDomain proteinsRegulatory hubDynein complexDynein isoformsDynein subunitsInsertional mutagenesisNew lociRegulatory proteinsDynein motorsDifferent dyneinsDoublet microtubulesTransport factorsPACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility
Dymek EE, Lin J, Fu G, Porter ME, Nicastro D, Smith EF. PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility. Molecular Biology Of The Cell 2019, 30: 1805-1816. PMID: 31116684, PMCID: PMC6727744, DOI: 10.1091/mbc.e19-01-0063.Peer-Reviewed Original ResearchMeSH KeywordsAlgal ProteinsAxonemeChlamydomonas reinhardtiiCiliaFlagellaMicrotubulesMovementMutationConceptsDoublet microtubulesAxonemal doublet microtubulesCryo-electron tomographyCiliary doublet microtubulesInner junctionCiliary assemblyCoordinated ciliary beatingFAP20Ciliary componentsMotility defectsCiliary beatingPACRGMotile ciliaFunctional studiesMutant axonemesMicrotubulesB-tubuleCiliary motilityEssential roleStructural studiesAssemblyMotilityChlamydomonasMutantsAxoneme
2018
The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility
Fu G, Wang Q, Phan N, Urbanska P, Joachimiak E, Lin J, Wloga D, Nicastro D. The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility. Molecular Biology Of The Cell 2018, 29: mbc.e18-02-0142. PMID: 29514928, PMCID: PMC5921572, DOI: 10.1091/mbc.e18-02-0142.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonemal DyneinsAxonemeChlamydomonas reinhardtiiCiliaCytoskeletonDyneinsFlagellaMicrotubulesSignal TransductionTetrahymenaConceptsI1 dyneinCiliary motilityAxonemal dynein motorsDynein motor domainMotor domainCiliary doublet microtubulesCK1 kinasesProper phosphorylationNormal ciliary motilityBiochemical approachesCryoelectron tomographyDynein motorsRegulatory mechanismsMotility regulatorMotile ciliaDoublet microtubulesRegulatorDyneinStable anchoringMotilityCiliary beatingComplexesHead complexMechanical feedbackImportant role
2015
DRC3 connects the N-DRC to dynein g to regulate flagellar waveform
Awata J, Song K, Lin J, King SM, Sanderson MJ, Nicastro D, Witman GB. DRC3 connects the N-DRC to dynein g to regulate flagellar waveform. Molecular Biology Of The Cell 2015, 26: 2788-2800. PMID: 26063732, PMCID: PMC4571338, DOI: 10.1091/mbc.e15-01-0018.Peer-Reviewed Original Research
2012
Conserved structural motifs in the central pair complex of eukaryotic flagella
Carbajal‐González B, Heuser T, Fu X, Lin J, Smith BW, Mitchell DR, Nicastro D. Conserved structural motifs in the central pair complex of eukaryotic flagella. Cytoskeleton 2012, 70: 101-120. PMID: 23281266, PMCID: PMC3914236, DOI: 10.1002/cm.21094.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological EvolutionChlamydomonas reinhardtiiCiliaFlagellaHumansMicrotubulesStrongylocentrotus purpuratusConceptsCentral pair complexCilia/flagellaRemarkable structural conservationGreen alga ChlamydomonasConserved Structural MotifCryo-electron tomographyOrganism-specific differencesEukaryotic cellsSea urchin StrongylocentrotusEvolutionary distanceStructural conservationAlga ChlamydomonasThree-dimensional structureSubtomogram averagingEukaryotic flagellaAxonemal dyneinsPrecise regulationInner proteinsMotility regulatorHair-like appendagesSinglet microtubulesChlamydomonasHuman diseasesDoublet microtubulesFlagellaThe CSC connects three major axonemal complexes involved in dynein regulation
Heuser T, Dymek EE, Lin J, Smith EF, Nicastro D. The CSC connects three major axonemal complexes involved in dynein regulation. Molecular Biology Of The Cell 2012, 23: 3143-3155. PMID: 22740634, PMCID: PMC3418309, DOI: 10.1091/mbc.e12-05-0357.Peer-Reviewed Original ResearchMeSH KeywordsAxonemeCalmodulinChlamydomonas reinhardtiiDyneinsFlagellaGene Knockdown TechniquesMicrotubulesPlant ProteinsProtein MultimerizationProtein Structure, QuaternaryRNA InterferenceConceptsNexin-dynein regulatory complexDynein regulationRadial spokesAxonemal complexCryo-electron tomographyWild-type motilityRegulatory complexFlagellar motilityDynein motorsChlamydomonas flagellaMotile ciliaStable assemblyFlagellaDifferent functionsRegulationRS1ComplexesMotilityStructural heterogeneityRS2Human healthImportant roleOrganellesHomologuesCSC
2011
Building Blocks of the Nexin-Dynein Regulatory Complex in Chlamydomonas Flagella*
Lin J, Tritschler D, Song K, Barber CF, Cobb JS, Porter ME, Nicastro D. Building Blocks of the Nexin-Dynein Regulatory Complex in Chlamydomonas Flagella*. Journal Of Biological Chemistry 2011, 286: 29175-29191. PMID: 21700706, PMCID: PMC3190725, DOI: 10.1074/jbc.m111.241760.Peer-Reviewed Original ResearchMeSH KeywordsAxonemeChlamydomonas reinhardtiiDyneinsFlagellaHumansMicrotubule-Associated ProteinsMutationPhosphorylationPlant ProteinsProteomicsConceptsNexin-dynein regulatory complexRegulatory complexImportant regulatory nodeTwo-dimensional electrophoresisRegulatory nodesProtein phosphorylationSignal transductionPhosphorylation statusMALDI-TOF mass spectrometryDynein motorsDynein activityChlamydomonas flagellaMotile ciliaRegulatory functionsPhosphorylated isoformsCoordination of thousandsFlagellar axonemeBiochemical comparisonRadial spokesPCR analysisFlagellar bendingIsoform patternFlagellaMolecular compositionProtein