Nikhil Singh, MD, PhD
Cards
About
Research
Publications
2021
Perianeurysmal retroperitoneal fibrosis presenting as acute kidney injury: A case report.
Hodson DZ, Cantley LG, Perincheri S, Singh N. Perianeurysmal retroperitoneal fibrosis presenting as acute kidney injury: A case report. Clinical Nephrology 2021, 96: 112-119. PMID: 34142944, DOI: 10.5414/cn110331.Peer-Reviewed Original ResearchConceptsAcute kidney injuryKidney injuryChronic periaortitisRenal functionRetroperitoneal fibrosisLong-term renal functionRetroperitoneal soft tissue massPerianeurysmal retroperitoneal fibrosisAcute tubular injuryLong-term sequelaeBilateral nephrostomy tubesInfrarenal abdominal aortaSoft tissue massBilateral uretersRenal ultrasoundCommon complicationOlder patientsRenal failureTubular injuryUltrasonographic evidenceUncommon causeRenal biopsyRenal sonographyFirst admissionObstructive nephropathyTubular Cell Dropout in Preimplantation Deceased Donor Biopsies as a Predictor of Delayed Graft Function
Avigan ZM, Singh N, Kliegel JA, Weiss M, Moeckel GW, Cantley LG. Tubular Cell Dropout in Preimplantation Deceased Donor Biopsies as a Predictor of Delayed Graft Function. Transplantation Direct 2021, 7: e716. PMID: 34476295, PMCID: PMC8384397, DOI: 10.1097/txd.0000000000001168.Peer-Reviewed Original ResearchDeceased donor kidney transplantDonor biopsiesGraft functionTubular injuryTubular cellsDelayed graft functionAcute tubular injuryDonor kidney transplantsConventional histopathologic evaluationPotential therapeutic interventionsMass cytometry analysisTubular cell lossDGF riskGraft outcomeTransplant recipientsKidney transplantPharmacologic therapyDeceased donorsMacrophage infiltrationHistopathologic evaluationLiving donorsDonor factorsCold ischemiaHistopathologic analysisCell dropout
2019
Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry
Singh N, Avigan ZM, Kliegel JA, Shuch BM, Montgomery RR, Moeckel GW, Cantley LG. Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry. JCI Insight 2019, 4: e129477. PMID: 31217358, PMCID: PMC6629112, DOI: 10.1172/jci.insight.129477.Peer-Reviewed Original ResearchConceptsCell typesIndividual cell typesCritical baseline dataRenal cell typesMass cytometryQuantitative atlasNormal human samplesHuman kidneyRelative abundanceDevelopment of therapiesHuman kidney diseaseKidney diseaseMetal-conjugated antibodiesQuantitative interrogationScarce samplesMachine-learning pipelineDiscovery purposesFuture quantitative analysisNovel abnormalityNormal human kidneySingle tissue sectionHuman samplesRenal biopsyImmune cellsCellsUrine TNF-α and IL-9 for clinical diagnosis of acute interstitial nephritis
Moledina DG, Wilson FP, Pober JS, Perazella MA, Singh N, Luciano RL, Obeid W, Lin H, Kuperman M, Moeckel GW, Kashgarian M, Cantley LG, Parikh CR. Urine TNF-α and IL-9 for clinical diagnosis of acute interstitial nephritis. JCI Insight 2019, 4: e127456. PMID: 31092735, PMCID: PMC6542610, DOI: 10.1172/jci.insight.127456.Peer-Reviewed Original ResearchConceptsAcute interstitial nephritisAcute kidney diseasePrebiopsy diagnosisKidney biopsyKidney diseaseIL-9AIN diagnosisUrine TNFInterstitial nephritisSpecific T cell subsetsAcute tubular injuryDiabetic kidney diseaseIL-9 levelsTNF-α levelsT cell subsetsAddition of biomarkersPlasma cytokinesCytokine levelsTubular injuryHighest quartileMultivariable analysisCell subsetsUrinary TNFBlood eosinophilsGlomerular disease
2013
Murine craniofacial development requires Hdac3-mediated repression of Msx gene expression
Singh N, Gupta M, Trivedi CM, Singh MK, Li L, Epstein JA. Murine craniofacial development requires Hdac3-mediated repression of Msx gene expression. Developmental Biology 2013, 377: 333-344. PMID: 23506836, PMCID: PMC3652235, DOI: 10.1016/j.ydbio.2013.03.008.Peer-Reviewed Original ResearchConceptsNeural crest cellsCrest cellsCraniofacial developmentNeural crestCranial neural crest cellsCore apoptotic pathwayMurine craniofacial developmentMsx gene expressionCleft secondary palateCell cycle genesCell cycle progressionEpigenomic levelsMutant embryosCraniofacial morphogenesisCycle genesCranial mesenchymeGenetic pathwaysGene expressionCycle progressionApoptotic pathwayMurine geneticsCritical regulatorHDAC3Mesodermal originSecondary palate
2011
Histone Deacetylase 3 Regulates Smooth Muscle Differentiation in Neural Crest Cells and Development of the Cardiac Outflow Tract
Singh N, Trivedi CM, Lu M, Mullican SE, Lazar MA, Epstein JA. Histone Deacetylase 3 Regulates Smooth Muscle Differentiation in Neural Crest Cells and Development of the Cardiac Outflow Tract. Circulation Research 2011, 109: 1240-1249. PMID: 21959220, PMCID: PMC3225257, DOI: 10.1161/circresaha.111.255067.Peer-Reviewed Original ResearchMeSH KeywordsAdrenal MedullaAnimalsAorta, ThoracicCell DifferentiationCell LineageCell MovementDouble Outlet Right VentricleFemaleFetal HeartGene Expression Regulation, DevelopmentalHeart Defects, CongenitalHeart Septal Defects, VentricularHeart VentriclesHistone DeacetylasesMaleMiceMice, TransgenicMuscle, SmoothNeural CrestPaired Box Transcription FactorsPAX3 Transcription FactorReceptors, NotchThymus GlandWnt1 ProteinConceptsNeural crest cellsCardiac outflow tractMuscle differentiationSmooth muscle differentiationCrest cellsCardiac neural crest developmentMultiple progenitor cell populationsFunctions of Hdac3Loss of Hdac3Neural crest cell migrationNeural crest developmentSmooth muscle lineageCrest cell migrationNumerous signaling pathwaysHistone deacetylase 3Role of epigeneticsProgenitor cell populationsSpecific regulatory roleNeural crest derivativesNotch ligand Jagged1OFT formationNeural crest resultsMammalian developmentMuscle lineageEpigenetic regulationDiet-induced Lethality Due to Deletion of the Hdac3 Gene in Heart and Skeletal Muscle
Sun Z, Singh N, Mullican SE, Everett LJ, Li L, Yuan L, Liu X, Epstein JA, Lazar MA. Diet-induced Lethality Due to Deletion of the Hdac3 Gene in Heart and Skeletal Muscle. Journal Of Biological Chemistry 2011, 286: 33301-33309. PMID: 21808063, PMCID: PMC3190900, DOI: 10.1074/jbc.m111.277707.Peer-Reviewed Original ResearchConceptsNutritional environmentProper mitochondrial functionHistone deacetylase 3Bioenergetic genesSkeletal muscleNucleosomal histonesHDAC3 geneGene expressionCovalent modificationHuman diseasesHDAC3Mitochondrial functionEmbryonic deletionCardiac mitochondriaGenesLipid metabolismDeletionMilder phenotypePhenotypeMajor cardiomyopathiesLethalityEpigenomeGenomeHistonesMouse model
2007
The effect of genetic background on the function of Saccharomyces cerevisiae mlh1 alleles that correspond to HNPCC missense mutations
Wanat JJ, Singh N, Alani E. The effect of genetic background on the function of Saccharomyces cerevisiae mlh1 alleles that correspond to HNPCC missense mutations. Human Molecular Genetics 2007, 16: 445-452. PMID: 17210669, DOI: 10.1093/hmg/ddl479.Peer-Reviewed Original ResearchConceptsHereditary non-polyposis colorectal cancerNon-polyposis colorectal cancerMMR defectsMismatch repair genes MLH1Functional assaysDNA mismatch repair genes MLH1Genetic backgroundColorectal cancerClinical dataMMR proficiencyMLH1 alleleGermline mutationsGenes MLH1Previous functional assaysMissense variantsMLH1Potential pathogenicityYeast Mlh1One-thirdMissense mutationsHuman mutationsCertain allelesLarge percentageAllelesPathogenicity
Others
- Singh N, Tucker MJ, Rieder MJ. Mechanisms of glucocorticoid anti-inflammatory and immunosuppressive action. Review. Paediatric and Perinatal Drug Therapy, April 2005; 6 (2).Peer-Reviewed Original Research
Links & Media
News
- September 16, 2021
Discoveries & Impact (September 2021)
- June 25, 2019Source: Technology Networks
Urine Biomarkers Could Improve Diagnosis of Serious Kidney Allergic Reaction
- June 06, 2019
Fellow Focus in Four (June)
- May 16, 2019Source: Eurekalert
Team finds biomarkers to diagnose serious kidney allergic reaction