Zhongyuan Zuo, MS
Senior Staff Scientist
Research & Publications
Biography
Research Summary
I am interested in solving biological questions researchers encountered with Electron Microscopy and volume Electron Microscopy technologies. Additionally, I employ AI assist Segmentation to label the region of interest, as well as extract the valuable data and 3D reconstruction from bulky FIB-SEM datasets. By providing the meaningful data analysis, I guided researchers to choose the combination EM technics and maximize/optimize their research findings.
Coauthors
Research Images
Selected Publications
- A defect in mitochondrial fatty acid synthesis impairs iron metabolism and causes elevated ceramide levelsDutta D, Kanca O, Byeon S, Marcogliese P, Zuo Z, Shridharan R, Park J, Lin G, Ge M, Heimer G, Kohler J, Wheeler M, Kaipparettu B, Pandey A, Bellen H. A defect in mitochondrial fatty acid synthesis impairs iron metabolism and causes elevated ceramide levels. Nature Metabolism 2023, 5: 1595-1614. PMID: 37653044, DOI: 10.1038/s42255-023-00873-0.
- Daam2 phosphorylation by CK2α negatively regulates Wnt activity during white matter development and injuryWang C, Zuo Z, Jo J, Kim K, Madamba C, Ye Q, Jung S, Bellen H, Lee H. Daam2 phosphorylation by CK2α negatively regulates Wnt activity during white matter development and injury. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2304112120. PMID: 37607236, PMCID: PMC10469030, DOI: 10.1073/pnas.2304112120.
- Very-long-chain fatty acids induce glial-derived sphingosine-1-phosphate synthesis, secretion, and neuroinflammationChung H, Ye Q, Park Y, Zuo Z, Mok J, Kanca O, Tattikota S, Lu S, Perrimon N, Lee H, Bellen H. Very-long-chain fatty acids induce glial-derived sphingosine-1-phosphate synthesis, secretion, and neuroinflammation. Cell Metabolism 2023, 35: 855-874.e5. PMID: 37084732, PMCID: PMC10160010, DOI: 10.1016/j.cmet.2023.03.022.
- Exploring therapeutic strategies for infantile neuronal axonal dystrophy (INAD/PARK14)Lin G, Tepe B, McGrane G, Tipon R, Croft G, Panwala L, Hope A, Liang A, Zuo Z, Byeon S, Wang L, Pandey A, Bellen H. Exploring therapeutic strategies for infantile neuronal axonal dystrophy (INAD/PARK14). ELife 2023, 12: e82555. PMID: 36645408, PMCID: PMC9889087, DOI: 10.7554/elife.82555.
- Two neuronal peptides encoded from a single transcript regulate mitochondrial complex III in DrosophilaBosch J, Ugur B, Pichardo-Casas I, Rabasco J, Escobedo F, Zuo Z, Brown B, Celniker S, Sinclair D, Bellen H, Perrimon N. Two neuronal peptides encoded from a single transcript regulate mitochondrial complex III in Drosophila. ELife 2022, 11: e82709. PMID: 36346220, PMCID: PMC9681215, DOI: 10.7554/elife.82709.
- Neuronal activity induces glucosylceramide that is secreted via exosomes for lysosomal degradation in gliaWang L, Lin G, Zuo Z, Li Y, Byeon S, Pandey A, Bellen H. Neuronal activity induces glucosylceramide that is secreted via exosomes for lysosomal degradation in glia. Science Advances 2022, 8: eabn3326. PMID: 35857503, PMCID: PMC9278864, DOI: 10.1126/sciadv.abn3326.
- An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombinationKanca O, Zirin J, Hu Y, Tepe B, Dutta D, Lin W, Ma L, Ge M, Zuo Z, Liu L, Levis R, Perrimon N, Bellen H. An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination. ELife 2022, 11: e76077. PMID: 35723254, PMCID: PMC9239680, DOI: 10.7554/elife.76077.
- Low doses of the organic insecticide spinosad trigger lysosomal defects, elevated ROS, lipid dysregulation, and neurodegeneration in fliesMartelli F, Hernandes N, Zuo Z, Wang J, Wong C, Karagas N, Roessner U, Rupasinghe T, Robin C, Venkatachalam K, Perry T, Batterham P, Bellen H. Low doses of the organic insecticide spinosad trigger lysosomal defects, elevated ROS, lipid dysregulation, and neurodegeneration in flies. ELife 2022, 11: e73812. PMID: 35191376, PMCID: PMC8863376, DOI: 10.7554/elife.73812.
- Daam2 Regulates Myelin Structure and the Oligodendrocyte Actin Cytoskeleton through Rac1 and Gelsolin.Cristobal C, Wang C, Zuo Z, Smith J, Lindeke-Myers A, Bellen H, Lee H. Daam2 Regulates Myelin Structure and the Oligodendrocyte Actin Cytoskeleton through Rac1 and Gelsolin. Journal Of Neuroscience 2022, 42: 1679-1691. PMID: 35101966, PMCID: PMC8896627, DOI: 10.1523/jneurosci.1517-21.2022.
- Loss of IRF2BPL impairs neuronal maintenance through excess Wnt signalingMarcogliese P, Dutta D, Ray S, Dang N, Zuo Z, Wang Y, Lu D, Fazal F, Ravenscroft T, Chung H, Kanca O, Wan J, Douine E, Network U, Pena L, Yamamoto S, Nelson S, Might M, Meyer K, Yeo N, Bellen H. Loss of IRF2BPL impairs neuronal maintenance through excess Wnt signaling. Science Advances 2022, 8: eabl5613. PMID: 35044823, PMCID: PMC8769555, DOI: 10.1126/sciadv.abl5613.
- TFEB/Mitf links impaired nuclear import to autophagolysosomal dysfunction in C9-ALSCunningham K, Maulding K, Ruan K, Senturk M, Grima J, Sung H, Zuo Z, Song H, Gao J, Dubey S, Rothstein J, Zhang K, Bellen H, Lloyd T. TFEB/Mitf links impaired nuclear import to autophagolysosomal dysfunction in C9-ALS. ELife 2020, 9: e59419. PMID: 33300868, PMCID: PMC7758070, DOI: 10.7554/elife.59419.
- Low doses of the neonicotinoid insecticide imidacloprid induce ROS triggering neurological and metabolic impairments in Drosophila.Martelli F, Zhongyuan Z, Wang J, Wong CO, Karagas NE, Roessner U, Rupasinghe T, Venkatachalam K, Perry T, Bellen HJ, Batterham P. Low doses of the neonicotinoid insecticide imidacloprid induce ROS triggering neurological and metabolic impairments in Drosophila. Proc Natl Acad Sci U S A 2020, 117: 25840-25850. PMID: 32989137, DOI: 10.1073/pnas.2011828117.
- The Daam2–VHL–Nedd4 axis governs developmental and regenerative oligodendrocyte differentiationDing X, Jo J, Wang C, Cristobal C, Zuo Z, Ye Q, Wirianto M, Lindeke-Myers A, Choi J, Mohila C, Kawabe H, Jung S, Bellen H, Yoo S, Lee H. The Daam2–VHL–Nedd4 axis governs developmental and regenerative oligodendrocyte differentiation. Genes & Development 2020, 34: 1177-1189. PMID: 32792353, PMCID: PMC7462057, DOI: 10.1101/gad.338046.120.
- Novel role of dynamin‐related‐protein 1 in dynamics of ER‐lipid droplets in adipose tissueLi X, Yang L, Mao Z, Pan X, Zhao Y, Gu X, Eckel‐Mahan K, Zuo Z, Tong Q, Hartig S, Cheng X, Du G, Moore D, Bellen H, Sesaki H, Sun K. Novel role of dynamin‐related‐protein 1 in dynamics of ER‐lipid droplets in adipose tissue. The FASEB Journal 2020, 34: 8265-8282. PMID: 32294302, PMCID: PMC7336545, DOI: 10.1096/fj.201903100rr.
- Retromer subunit, VPS29, regulates synaptic transmission and is required for endolysosomal function in the aging brainYe H, Ojelade S, Li-Kroeger D, Zuo Z, Wang L, Li Y, Gu J, Tepass U, Rodal A, Bellen H, Shulman J. Retromer subunit, VPS29, regulates synaptic transmission and is required for endolysosomal function in the aging brain. ELife 2020, 9: e51977. PMID: 32286230, PMCID: PMC7182434, DOI: 10.7554/elife.51977.
- Loss- or Gain-of-Function Mutations in ACOX1 Cause Axonal Loss via Different MechanismsChung H, Wangler M, Marcogliese P, Jo J, Ravenscroft T, Zuo Z, Duraine L, Sadeghzadeh S, Li-Kroeger D, Schmidt R, Pestronk A, Rosenfeld J, Burrage L, Herndon M, Chen S, Network M, Shillington A, Vawter-Lee M, Hopkin R, Rodriguez-Smith J, Henrickson M, Lee B, Moser A, Jones R, Watkins P, Yoo T, Mar S, Choi M, Bucelli R, Yamamoto S, Lee H, Prada C, Chae J, Vogel T, Bellen H. Loss- or Gain-of-Function Mutations in ACOX1 Cause Axonal Loss via Different Mechanisms. Neuron 2020, 106: 589-606.e6. PMID: 32169171, PMCID: PMC7289150, DOI: 10.1016/j.neuron.2020.02.021.
- Loss of Oxidation Resistance 1, OXR1, Is Associated with an Autosomal-Recessive Neurological Disease with Cerebellar Atrophy and Lysosomal DysfunctionWang J, Rousseau J, Kim E, Ehresmann S, Cheng Y, Duraine L, Zuo Z, Park Y, Li-Kroeger D, Bi W, Wong L, Rosenfeld J, Gleeson J, Faqeih E, Alkuraya F, Wierenga K, Chen J, Afenjar A, Nava C, Doummar D, Keren B, Juusola J, Grompe M, Bellen H, Campeau P. Loss of Oxidation Resistance 1, OXR1, Is Associated with an Autosomal-Recessive Neurological Disease with Cerebellar Atrophy and Lysosomal Dysfunction. American Journal Of Human Genetics 2019, 105: 1237-1253. PMID: 31785787, PMCID: PMC6904826, DOI: 10.1016/j.ajhg.2019.11.002.
- An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology armsKanca O, Zirin J, Garcia-Marques J, Knight S, Yang-Zhou D, Amador G, Chung H, Zuo Z, Ma L, He Y, Lin W, Fang Y, Ge M, Yamamoto S, Schulze K, Hu Y, Spradling A, Mohr S, Perrimon N, Bellen H. An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms. ELife 2019, 8: e51539. PMID: 31674908, PMCID: PMC6855806, DOI: 10.7554/elife.51539.
- cindr, the Drosophila Homolog of the CD2AP Alzheimer’s Disease Risk Gene, Is Required for Synaptic Transmission and ProteostasisOjelade S, Lee T, Giagtzoglou N, Yu L, Ugur B, Li Y, Duraine L, Zuo Z, Petyuk V, De Jager P, Bennett D, Arenkiel B, Bellen H, Shulman J. cindr, the Drosophila Homolog of the CD2AP Alzheimer’s Disease Risk Gene, Is Required for Synaptic Transmission and Proteostasis. Cell Reports 2019, 28: 1799-1813.e5. PMID: 31412248, PMCID: PMC6703184, DOI: 10.1016/j.celrep.2019.07.041.
- Ubiquilins regulate autophagic flux through mTOR signalling and lysosomal acidificationŞentürk M, Lin G, Zuo Z, Mao D, Watson E, Mikos A, Bellen H. Ubiquilins regulate autophagic flux through mTOR signalling and lysosomal acidification. Nature Cell Biology 2019, 21: 384-396. PMID: 30804504, PMCID: PMC6534127, DOI: 10.1038/s41556-019-0281-x.
- VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathwayMao D, Lin G, Tepe B, Zuo Z, Tan K, Senturk M, Zhang S, Arenkiel B, Sardiello M, Bellen H. VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway. Autophagy 2019, 15: 1214-1233. PMID: 30741620, PMCID: PMC6613884, DOI: 10.1080/15548627.2019.1580103.
- Molecular layer interneurons shape the spike activity of cerebellar Purkinje cellsBrown A, Arancillo M, Lin T, Catt D, Zhou J, Lackey E, Stay T, Zuo Z, White J, Sillitoe R. Molecular layer interneurons shape the spike activity of cerebellar Purkinje cells. Scientific Reports 2019, 9: 1742. PMID: 30742002, PMCID: PMC6370775, DOI: 10.1038/s41598-018-38264-1.
- An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in DrosophilaLi-Kroeger D, Kanca O, Lee P, Cowan S, Lee M, Jaiswal M, Salazar J, He Y, Zuo Z, Bellen H. An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila. ELife 2018, 7: e38709. PMID: 30091705, PMCID: PMC6095692, DOI: 10.7554/elife.38709.
- IRF2BPL Is Associated with Neurological PhenotypesMarcogliese P, Shashi V, Spillmann R, Stong N, Rosenfeld J, Koenig M, Martínez-Agosto J, Herzog M, Chen A, Dickson P, Lin H, Vera M, Salamon N, Graham J, Ortiz D, Infante E, Steyaert W, Dermaut B, Poppe B, Chung H, Zuo Z, Lee P, Kanca O, Xia F, Yang Y, Smith E, Jasien J, Kansagra S, Spiridigliozzi G, El-Dairi M, Lark R, Riley K, Koeberl D, Golden-Grant K, Diseases P, Callens S, Coucke P, Dermaut B, Hemelsoet D, Poppe B, Steyaert W, Terryn W, Van Coster R, Network U, Adams D, Alejandro M, Allard P, Azamian M, Bacino C, Balasubramanyam A, Barseghyan H, Batzli G, Beggs A, Behnam B, Bican A, Bick D, Birch C, Bonner D, Boone B, Bostwick B, Briere L, Brown D, Brush M, Burke E, Burrage L, Chen S, Clark G, Coakley T, Cogan J, Cooper C, Cope H, Craigen W, D’Souza P, Davids M, Dayal J, Dell’Angelica E, Dhar S, Dillon A, Dipple K, Donnell-Fink L, Dorrani N, Dorset D, Douine E, Draper D, Eckstein D, Emrick L, Eng C, Eskin A, Esteves C, Estwick T, Ferreira C, Fogel B, Friedman N, Gahl W, Glanton E, Godfrey R, Goldstein D, Gould S, Gourdine J, Groden C, Gropman A, Haendel M, Hamid R, Hanchard N, Handley L, Herzog M, Holm I, Hom J, Howerton E, Huang Y, Jacob H, Jain M, Jiang Y, Johnston J, Jones A, Kohane I, Krasnewich D, Krieg E, Krier J, Lalani S, Lau C, Lazar J, Lee B, Lee H, Levy S, Lewis R, Lincoln S, Lipson A, Loo S, Loscalzo J, Maas R, Macnamara E, MacRae C, Maduro V, Majcherska M, Malicdan M, Mamounas L, Manolio T, Markello T, Marom R, Martínez-Agosto J, Marwaha S, May T, McConkie-Rosell A, McCormack C, McCray A, Might M, Moretti P, Morimoto M, Mulvihill J, Murphy J, Muzny D, Nehrebecky M, Nelson S, Newberry J, Newman J, Nicholas S, Novacic D, Orange J, Pallais J, Palmer C, Papp J, Parker N, Pena L, Phillips J, Posey J, Postlethwait J, Potocki L, Pusey B, Reuter C, Robertson A, Rodan L, Rosenfeld J, Sampson J, Samson S, Schoch K, Schroeder M, Scott D, Sharma P, Shashi V, Signer R, Silverman E, Sinsheimer J, Smith K, Spillmann R, Splinter K, Stoler J, Stong N, Sullivan J, Sweetser D, Tifft C, Toro C, Tran A, Urv T, Valivullah Z, Vilain E, Vogel T, Wahl C, Walley N, Walsh C, Ward P, Waters K, Westerfield M, Wise A, Wolfe L, Worthey E, Yamamoto S, Yang Y, Yu G, Zastrow D, Zheng A, Yamamoto S, Wangler M, Mirzaa G, Hemelsoet D, Lee B, Nelson S, Goldstein D, Bellen H, Pena L. IRF2BPL Is Associated with Neurological Phenotypes. American Journal Of Human Genetics 2018, 103: 245-260. PMID: 30057031, PMCID: PMC6081494, DOI: 10.1016/j.ajhg.2018.07.006.
- Phospholipase PLA2G6, a Parkinsonism-Associated Gene, Affects Vps26 and Vps35, Retromer Function, and Ceramide Levels, Similar to α-Synuclein GainLin G, Lee P, Chen K, Mao D, Tan K, Zuo Z, Lin W, Wang L, Bellen H. Phospholipase PLA2G6, a Parkinsonism-Associated Gene, Affects Vps26 and Vps35, Retromer Function, and Ceramide Levels, Similar to α-Synuclein Gain. Cell Metabolism 2018, 28: 605-618.e6. PMID: 29909971, DOI: 10.1016/j.cmet.2018.05.019.
- A gene-specific T2A-GAL4 library for DrosophilaLee P, Zirin J, Kanca O, Lin W, Schulze K, Li-Kroeger D, Tao R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, Housden B, Mohr S, Yamamoto S, Levis R, Spradling A, Perrimon N, Bellen H. A gene-specific T2A-GAL4 library for Drosophila. ELife 2018, 7: e35574. PMID: 29565247, PMCID: PMC5898912, DOI: 10.7554/elife.35574.
- The Krebs Cycle Enzyme Isocitrate Dehydrogenase 3A Couples Mitochondrial Metabolism to Synaptic TransmissionUgur B, Bao H, Stawarski M, Duraine LR, Zuo Z, Lin YQ, Neely GG, Macleod GT, Chapman ER, Bellen HJ. The Krebs Cycle Enzyme Isocitrate Dehydrogenase 3A Couples Mitochondrial Metabolism to Synaptic Transmission. Cell Reports 2017, 21: 3794-3806. PMID: 29281828, PMCID: PMC5747319, DOI: 10.1016/j.celrep.2017.12.005.
- Clinically severe CACNA1A alleles affect synaptic function and neurodegeneration differentiallyLuo X, Rosenfeld J, Yamamoto S, Harel T, Zuo Z, Hall M, Wierenga K, Pastore M, Bartholomew D, Delgado M, Rotenberg J, Lewis R, Emrick L, Bacino C, Eldomery M, Coban Akdemir Z, Xia F, Yang Y, Lalani S, Lotze T, Lupski J, Lee B, Bellen H, Wangler M, . Clinically severe CACNA1A alleles affect synaptic function and neurodegeneration differentially. PLOS Genetics 2017, 13: e1006905. PMID: 28742085, PMCID: PMC5557584, DOI: 10.1371/journal.pgen.1006905.
- A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cellsNagarkar-Jaiswal S, Manivannan S, Zuo Z, Bellen H. A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells. ELife 2017, 6: e26420. PMID: 28561736, PMCID: PMC5493436, DOI: 10.7554/elife.26420.
- Uncoupling neuronal death and dysfunction in Drosophila models of neurodegenerative diseaseChouhan A, Guo C, Hsieh Y, Ye H, Senturk M, Zuo Z, Li Y, Chatterjee S, Botas J, Jackson G, Bellen H, Shulman J. Uncoupling neuronal death and dysfunction in Drosophila models of neurodegenerative disease. Acta Neuropathologica Communications 2016, 4: 62. PMID: 27338814, PMCID: PMC4918017, DOI: 10.1186/s40478-016-0333-4.
- Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogasterMavor L, Miao H, Zuo Z, Holly R, Xie Y, Loerke D, Blankenship J. Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogaster. Journal Of Cell Science 2016, 129: e1.1-e1.1. DOI: 10.1242/jcs.188474.
- WAC Regulates mTOR Activity by Acting as an Adaptor for the TTT and Pontin/Reptin ComplexesDavid-Morrison G, Xu Z, Rui Y, Charng W, Jaiswal M, Yamamoto S, Xiong B, Zhang K, Sandoval H, Duraine L, Zuo Z, Zhang S, Bellen H. WAC Regulates mTOR Activity by Acting as an Adaptor for the TTT and Pontin/Reptin Complexes. Developmental Cell 2016, 36: 139-151. PMID: 26812014, PMCID: PMC4730548, DOI: 10.1016/j.devcel.2015.12.019.
- Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogasterMavor L, Miao H, Zuo Z, Holly R, Xie Y, Loerke D, Blankenship J. Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogaster. Development 2016, 143: 892-903. PMID: 26839362, PMCID: PMC4813336, DOI: 10.1242/dev.128876.
- A rapid, membrane-dependent pathway directs furrow formation through RalA in the early Drosophila embryoHolly R, Mavor L, Zuo Z, Blankenship J. A rapid, membrane-dependent pathway directs furrow formation through RalA in the early Drosophila embryo. Journal Of Cell Science 2015, 128: e1.1-e1.1. DOI: 10.1242/jcs.176560.
- A genetic toolkit for tagging intronic MiMIC containing genesNagarkar-Jaiswal S, DeLuca S, Lee P, Lin W, Pan H, Zuo Z, Lv J, Spradling A, Bellen H. A genetic toolkit for tagging intronic MiMIC containing genes. ELife 2015, 4: e08469. PMID: 26102525, PMCID: PMC4499919, DOI: 10.7554/elife.08469.
- A rapid, membrane-dependent pathway directs furrow formation through RalA in the early Drosophila embryoHolly R, Mavor L, Zuo Z, Blankenship J. A rapid, membrane-dependent pathway directs furrow formation through RalA in the early Drosophila embryo. Development 2015, 142: 2316-2328. PMID: 26092850, PMCID: PMC4510590, DOI: 10.1242/dev.120998.