Peter Tattersall, PhD
Research & Publications
Biography
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Research Summary
Our research efforts are directed at understanding the molecular mechanisms by which mammalian parvoviruses target particular cell types, express their genes, take over their host cells and replicate their own DNA. Eukaryotic and prokaryotic expression systems, coupled with directed mutagenesis, are currently being used to separate the various functions of the complex, multi-functional parvoviral gene products, in order to understand how the virus subverts the macromolecular metabolism of its target host cell to its own ends. We are currently applying this knowledge to the construction and validation of vectors designed to target tumor cells, and deliver immunomodulatory proteins directly within the tumor microenvironment, initially as a therapeutic strategy against melanoma.
Specialized Terms: Biochemistry; Genetics; Virology; DNA replication; Oncolytic virus; Vaccines; Vectors; Melanoma
Extensive Research Description
Manipulating the oncoselectivity of parvoviruses in human tumor models
Most of the rodent parvoviruses will bind to and enter human cells with high efficiency, but fail to initiate gene expression, replicate their genomes, generate progeny or spread through the culture, unless the host cell is neoplastically transformed. As a consequence, these viruses are promising candidates as oncolytic agents for cancer therapy, particularly in situations where other treatments have proven ineffective. Our current efforts are directed toward understanding, at the molecular level, why cellular changes that accompany oncogenic transformation promote viral growth, and how we can use this knowledge to further improve the efficacy of the virus in tumor eradication. Since tumorigenesis normally involves loss of genomic integrity, tumor cells carry many mutations that are secondary to those causing the transformed phenotype. To avoid studying or selecting for viral traits that represent adaptations to such “collateral” transformed cell properties, we are using host cells that have been transformed in a stepwise fashion with activated oncogenes and/or tumor suppressor knock-downs. Currently we are exploring the contribution of the viral capsid and initiating promoter to the discrimination between normal and transformed cells, using stepwise transformed human fibroblasts and melanocytes, the latter being a model for malignant melanoma. These studies are directing strategies for selecting more oncotropic versions of these critical oncoselective elements, using gene shuffling and degenerate promoter library approaches.
Induction of immunogenic cell death by oncosuppressive parvoviruses
Many of the autonomously replicating rodent parvoviruses can enter human cells, generate progeny and spread through the culture only if the host cell is neoplastically transformed, making these viruses promising candidates as oncolytic agents. Parvoviral induction of complete tumor regression has been achieved in several syngeneic transplantable tumor models in immunocompetent rodent hosts, and often results in immunization of the animal against subsequent transplantation of cells of the same tumor, even at high input numbers, suggesting that some aspect of parvovirus infection elicits a strong anti-tumor immune response. This project utilizes a mouse melanoma model system to explore whether parvovirus-induced cell death proceeds via an immunogenic, rather than tolerogenic, pathway, by examining the expression of phagocytic engulfment signals on the infected cell surface, coupled with the secretion of soluble damage-associated molecular pattern (DAMP) molecules, such as HMGB1 and Hsp72.
How parvoviruses enter their host cell and traffic to the nucleus
Parvoviruses do not have a lipid envelope, and so cannot deliver their virions into the host cell by fusing with its plasma or endosomal membranes. These viruses have developed an alternative strategy to breach their host cell's outer membrane and gain entry into the cytoplasm. We have shown that the compact, icosahedral virion of the murine parvovirus Minute Virus of Mice, MVM deploys a lipolytic enzyme, phospholipase A2 (PLA2) that is expressed at the N-terminus of the minor coat protein, VP1. This region of VP1 is normally sequestered within the viral shell, but is extruded during the entry process as a capsid-tethered domain, via an 8Å pore that extends through the prominent 5-fold cylinder. [Figure] In addition to the PLA2 domain, the extruded VP1 N-terminus also displays a number of small protein interaction domains predicted to engage both ubiquitin ligases of the NEDD4 family, involved in endocytosis and vesicle trafficking, and nuclear transport proteins of the alpha-importin family. The sequential conformational shifts within the particle that allow these transitions to occur as the virion transits its entry pathway, exposing first its VP2 N-termini, then its VP1 N-termini and ultimately its DNA are being analyzed using X-ray crystallography and asymmetric cryo-electron microscopy, in collaborations with Drs. Susan Hafenstein at Hershey Medical School and Mavis Agbandje-McKenna at the University of Florida, Gainesville. Finally, we are using reverse genetics combined with differential real-time PCR, sub-cellular fractionation and in situ imaging techniques, such as Proximity Ligation, to explore the roles of the VP1 N-terminal domain in the trans-cytosolic trafficking and nuclear import of MVM virions.
Characterizing the unique chromatin assembled during parvoviral DNA replication
During the S-phase following infection, autonomous parvoviruses inveigle host cells to replicate the linear, single-stranded viral DNA chromosome, instead of the cellular genome. Part of the virus’ replication strategy involves the elaboration of a unique form of chromatin, which ChIP analysis suggests incorporates both cellular histones and many copies of NS1, the major viral non-structural protein. This project explores the replication of an otherwise wildtype viral genome rendered artificially devoid of NS1 binding sites throughout its entire NS1 gene, capsid gene and/or 3’ untranslated region. 2D gel electrophoresis and nuclease protection assays will be used to look for stalling or pausing of replication forks through the capsid region, and to characterize packaging intermediates generated by mutant, compared to wildtype, virus.
Research Interests
Dependovirus; Biochemistry; Eukaryotic Cells; Genetics; Melanoma, Experimental; Mice, Mutant Strains; Medical Laboratory Science; Parvovirus; Erythrovirus; Oncolytic Virotherapy; Oncolytic Viruses; Mice; Bocavirus; Viral Structures; Immunomodulation
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Selected Publications
- The Nature of ParvovirusesTattersall P, Cotmore S. The Nature of Parvoviruses. 2018, 5-41. DOI: 10.1201/9781351075404-2.
- Cryo-EM maps reveal five-fold channel structures and their modification by gatekeeper mutations in the parvovirus minute virus of mice (MVM) capsidSubramanian S, Organtini LJ, Grossman A, Domeier PP, Cifuente JO, Makhov AM, Conway JF, D'Abramo A, Cotmore SF, Tattersall P, Hafenstein S. Cryo-EM maps reveal five-fold channel structures and their modification by gatekeeper mutations in the parvovirus minute virus of mice (MVM) capsid. Virology 2017, 510: 216-223. PMID: 28750325, PMCID: PMC5601314, DOI: 10.1016/j.virol.2017.07.015.
- The MVMp P4 promoter is a host cell-type range determinant in vivoMeir C, Mincberg M, Rostovsky I, Tal S, Vollmers EM, Levi A, Tattersall P, Davis C. The MVMp P4 promoter is a host cell-type range determinant in vivo. Virology 2017, 506: 141-151. PMID: 28391161, DOI: 10.1016/j.virol.2017.03.012.
- Complementation for an essential ancillary non-structural protein function across parvovirus generaMihaylov IS, Cotmore SF, Tattersall P. Complementation for an essential ancillary non-structural protein function across parvovirus genera. Virology 2014, 468: 226-237. PMID: 25194919, PMCID: PMC4254310, DOI: 10.1016/j.virol.2014.07.043.
- Parvoviridae: General Features☆Cotmore S, Tattersall P. Parvoviridae: General Features☆. 2014 DOI: 10.1016/b978-0-12-801238-3.02641-6.
- Distinct host cell fates for human malignant melanoma targeted by oncolytic rodent parvovirusesVollmers EM, Tattersall P. Distinct host cell fates for human malignant melanoma targeted by oncolytic rodent parvoviruses. Virology 2013, 446: 37-48. PMID: 24074565, PMCID: PMC3811133, DOI: 10.1016/j.virol.2013.07.013.
- Parvoviral Left-End Hairpin Ears Are Essential during Infection for Establishing a Functional Intranuclear Transcription Template and for Efficient Progeny Genome EncapsidationLi L, Cotmore SF, Tattersall P. Parvoviral Left-End Hairpin Ears Are Essential during Infection for Establishing a Functional Intranuclear Transcription Template and for Efficient Progeny Genome Encapsidation. Journal Of Virology 2013, 87: 10501-10514. PMID: 23903839, PMCID: PMC3807388, DOI: 10.1128/jvi.01393-13.
- Parvovirus evades interferon-dependent viral control in primary mouse embryonic fibroblastsMattei LM, Cotmore SF, Tattersall P, Iwasaki A. Parvovirus evades interferon-dependent viral control in primary mouse embryonic fibroblasts. Virology 2013, 442: 20-27. PMID: 23676303, PMCID: PMC3767977, DOI: 10.1016/j.virol.2013.03.020.
- Mutations at the Base of the Icosahedral Five-Fold Cylinders of Minute Virus of Mice Induce 3′-to-5′ Genome Uncoating and Critically Impair Entry FunctionsCotmore SF, Tattersall P. Mutations at the Base of the Icosahedral Five-Fold Cylinders of Minute Virus of Mice Induce 3′-to-5′ Genome Uncoating and Critically Impair Entry Functions. Journal Of Virology 2011, 86: 69-80. PMID: 22013064, PMCID: PMC3255873, DOI: 10.1128/jvi.06119-11.
- The parvoviral capsid controls an intracellular phase of infection essential for efficient killing of stepwise-transformed human fibroblastsPaglino J, Tattersall P. The parvoviral capsid controls an intracellular phase of infection essential for efficient killing of stepwise-transformed human fibroblasts. Virology 2011, 416: 32-41. PMID: 21600623, PMCID: PMC3112476, DOI: 10.1016/j.virol.2011.04.015.
- Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold AxisPlevka P, Hafenstein S, Li L, D'Abrgamo A, Cotmore SF, Rossmann MG, Tattersall P. Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis. Journal Of Virology 2011, 85: 4822-4827. PMID: 21367911, PMCID: PMC3126206, DOI: 10.1128/jvi.02598-10.
- Recruitment of DNA replication and damage response proteins to viral replication centers during infection with NS2 mutants of Minute Virus of Mice (MVM)Ruiz Z, Mihaylov IS, Cotmore SF, Tattersall P. Recruitment of DNA replication and damage response proteins to viral replication centers during infection with NS2 mutants of Minute Virus of Mice (MVM). Virology 2010, 410: 375-384. PMID: 21193212, PMCID: PMC3072075, DOI: 10.1016/j.virol.2010.12.009.
- ParvovirusesTattersall P, Cotmore S. Parvoviruses. 2010 DOI: 10.1002/9780470688618.taw0227.
- Human Bocavirus: a Newly Discovered Human ParvovirusKahn J, Tattersall P. Human Bocavirus: a Newly Discovered Human Parvovirus. 2008, 21-38. DOI: 10.1128/9781555815592.ch2.
- Parvoviruses: General FeaturesTattersall P. Parvoviruses: General Features. 2008, 90-97. DOI: 10.1016/b978-012374410-4.00463-5.
- Structure and organization of the viral genomeCotmore S, Tattersall P. Structure and organization of the viral genome. 2005, 73-94. DOI: 10.1201/b13393-11.
- A rolling-hairpin strategyCotmore S, Tattersall P. A rolling-hairpin strategy. 2005, 171-188. DOI: 10.1201/b13393-19.
- IntroductionCotmore S, Tattersall P. Introduction. 2005, 71-72. DOI: 10.1201/b13393-10.
- ParvovirusesTattersall P, Cotmore S. Parvoviruses. 2003 DOI: 10.1038/npg.els.0000423.
- A consensus DNA recognition motif for two KDWK transcription factors identifies flexible-length, CpG-methylation sensitive cognate binding sites in the majority of human promoters11Edited by M. YanivBurnett E, Christensen J, Tattersall P. A consensus DNA recognition motif for two KDWK transcription factors identifies flexible-length, CpG-methylation sensitive cognate binding sites in the majority of human promoters11Edited by M. Yaniv. Journal Of Molecular Biology 2001, 314: 1029-1039. PMID: 11743720, DOI: 10.1006/jmbi.2000.5198.
- The Left-End and Right-End Origins of Minute Virus of Mice DNA Differ in Their Capacity to Direct Episomal Amplification and Integration In VivoCorsini J, Cotmore S, Tattersall P, Winocour E. The Left-End and Right-End Origins of Minute Virus of Mice DNA Differ in Their Capacity to Direct Episomal Amplification and Integration In Vivo. Virology 2001, 288: 154-163. PMID: 11543668, DOI: 10.1006/viro.2001.1076.
- Minute Virus of Mice Initiator Protein NS1 and a Host KDWK Family Transcription Factor Must Form a Precise Ternary Complex with Origin DNA for Nicking To OccurChristensen J, Cotmore S, Tattersall P. Minute Virus of Mice Initiator Protein NS1 and a Host KDWK Family Transcription Factor Must Form a Precise Ternary Complex with Origin DNA for Nicking To Occur. Journal Of Virology 2001, 75: 7009-7017. PMID: 11435581, PMCID: PMC114429, DOI: 10.1128/jvi.75.15.7009-7017.2001.
- The persistence of alien genomesTattersall P. The persistence of alien genomes. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 6239-6241. PMID: 10841525, PMCID: PMC33992, DOI: 10.1073/pnas.97.12.6239.
- Two Widely Spaced Initiator Binding Sites Create an HMG1-Dependent Parvovirus Rolling-Hairpin Replication OriginCotmore S, Christensen J, Tattersall P. Two Widely Spaced Initiator Binding Sites Create an HMG1-Dependent Parvovirus Rolling-Hairpin Replication Origin. Journal Of Virology 2000, 74: 1332-1341. PMID: 10627544, PMCID: PMC111468, DOI: 10.1128/jvi.74.3.1332-1341.2000.
- Autonomous Parvoviruses as Gene Transfer VehiclesPalmer G, Tattersall P. Autonomous Parvoviruses as Gene Transfer Vehicles. 2000, 4: 178-202. PMID: 10941578, DOI: 10.1159/000060337.
- Two New Members of the Emerging KDWK Family of Combinatorial Transcription Modulators Bind as a Heterodimer to Flexibly Spaced PuCGPy Half-SitesChristensen J, Cotmore S, Tattersall P. Two New Members of the Emerging KDWK Family of Combinatorial Transcription Modulators Bind as a Heterodimer to Flexibly Spaced PuCGPy Half-Sites. Molecular And Cellular Biology 1999, 19: 7741-7750. PMID: 10523663, PMCID: PMC84824, DOI: 10.1128/mcb.19.11.7741.
- cis Requirements for the Efficient Production of Recombinant DNA Vectors Based on Autonomous ParvovirusesKestler J, Neeb B, Struyf S, Van Damme J, Cotmore S, D'Abramo A, Tattersall P, Rommelaere J, Dinsart C, Cornelis J. cis Requirements for the Efficient Production of Recombinant DNA Vectors Based on Autonomous Parvoviruses. Human Gene Therapy 1999, 10: 1619-1632. PMID: 10428207, DOI: 10.1089/10430349950017626.
- Controlled Conformational Transitions in the MVM Virion Expose the VP1 N-Terminus and Viral Genome without Particle DisassemblyCotmore S, D'Abramo A, Ticknor C, Tattersall P. Controlled Conformational Transitions in the MVM Virion Expose the VP1 N-Terminus and Viral Genome without Particle Disassembly. Virology 1999, 254: 169-181. PMID: 9927584, DOI: 10.1006/viro.1998.9520.
- High-mobility group 1/2 proteins are essential for initiating rolling-circle-type DNA replication at a parvovirus hairpin origin.Cotmore S, Tattersall P. High-mobility group 1/2 proteins are essential for initiating rolling-circle-type DNA replication at a parvovirus hairpin origin. Journal Of Virology 1998, 72: 8477-84. PMID: 9765384, PMCID: PMC110256, DOI: 10.1128/jvi.72.11.8477-8484.1998.
- Functional implications of the structure of the murine parvovirus, minute virus of miceAgbandje-McKenna M, Llamas-Saiz A, Wang F, Tattersall P, Rossmann M. Functional implications of the structure of the murine parvovirus, minute virus of mice. Structure 1998, 6: 1369-1381. PMID: 9817841, DOI: 10.1016/s0969-2126(98)00137-3.
- Biochemical activities of minute virus of mice nonstructural protein NS1 are modulated In vitro by the phosphorylation state of the polypeptide.Nüesch J, Corbau R, Tattersall P, Rommelaere J. Biochemical activities of minute virus of mice nonstructural protein NS1 are modulated In vitro by the phosphorylation state of the polypeptide. Journal Of Virology 1998, 72: 8002-12. PMID: 9733839, PMCID: PMC110136, DOI: 10.1128/jvi.72.10.8002-8012.1998.
- Parvovirus initiation factor PIF: a novel human DNA-binding factor which coordinately recognizes two ACGT motifs.Christensen J, Cotmore S, Tattersall P. Parvovirus initiation factor PIF: a novel human DNA-binding factor which coordinately recognizes two ACGT motifs. Journal Of Virology 1997, 71: 5733-41. PMID: 9223459, PMCID: PMC191825, DOI: 10.1128/jvi.71.8.5733-5741.1997.
- Genetically engineered chimeric viruses for the treatment of diseases associated with viral transactivatorsTattersall P, Cotmore S. Genetically engineered chimeric viruses for the treatment of diseases associated with viral transactivators. Biofutur 1997, 1997: 47. DOI: 10.1016/s0294-3506(99)80353-6.
- The NS2 Polypeptide of Parvovirus MVM Is Required for Capsid Assembly in Murine CellsCotmore S, D'abramo A, Carbonell L, Bratton J, Tattersall P. The NS2 Polypeptide of Parvovirus MVM Is Required for Capsid Assembly in Murine Cells. Virology 1997, 231: 267-280. PMID: 9168889, DOI: 10.1006/viro.1997.8545.
- A novel cellular site-specific DNA-binding protein cooperates with the viral NS1 polypeptide to initiate parvovirus DNA replication.Christensen J, Cotmore S, Tattersall P. A novel cellular site-specific DNA-binding protein cooperates with the viral NS1 polypeptide to initiate parvovirus DNA replication. Journal Of Virology 1997, 71: 1405-16. PMID: 8995666, PMCID: PMC191197, DOI: 10.1128/jvi.71.2.1405-1416.1997.
- Structure Determination of Minute Virus of MiceLlamas-Saiz A, Agbandje-McKenna M, Wikoff W, Bratton J, Tattersall P, Rossmann M. Structure Determination of Minute Virus of Mice. Acta Crystallographica Section D, Structural Biology 1997, 53: 93-102. PMID: 15299974, DOI: 10.1107/s0907444996010566.
- DNA replication in the autonomous parvovirusesCotmore S, Tattersall* P. DNA replication in the autonomous parvoviruses. Seminars In Virology 1995, 6: 271-281. DOI: 10.1006/smvy.1995.0033.
- Minute virus of mice transcriptional activator protein NS1 binds directly to the transactivation region of the viral P38 promoter in a strictly ATP-dependent manner.Christensen J, Cotmore S, Tattersall P. Minute virus of mice transcriptional activator protein NS1 binds directly to the transactivation region of the viral P38 promoter in a strictly ATP-dependent manner. Journal Of Virology 1995, 69: 5422-30. PMID: 7636987, PMCID: PMC189388, DOI: 10.1128/jvi.69.9.5422-5430.1995.
- Sequence Motifs in the Replicator Protein of Parvovirus MVM Essential for Nicking and Covalent Attachment to the Viral Origin: Identification of the Linking TyrosineNüesch J, Cotmore S, Tattersall P. Sequence Motifs in the Replicator Protein of Parvovirus MVM Essential for Nicking and Covalent Attachment to the Viral Origin: Identification of the Linking Tyrosine. Virology 1995, 209: 122-135. PMID: 7747462, DOI: 10.1006/viro.1995.1236.
- The NS1 polypeptide of the murine parvovirus minute virus of mice binds to DNA sequences containing the motif [ACCA]2-3.Cotmore S, Christensen J, Nüesch J, Tattersall P. The NS1 polypeptide of the murine parvovirus minute virus of mice binds to DNA sequences containing the motif [ACCA]2-3. Journal Of Virology 1995, 69: 1652-60. PMID: 7853501, PMCID: PMC188764, DOI: 10.1128/jvi.69.3.1652-1660.1995.
- An asymmetric nucleotide in the parvoviral 3' hairpin directs segregation of a single active origin of DNA replication.Cotmore S, Tattersall P. An asymmetric nucleotide in the parvoviral 3' hairpin directs segregation of a single active origin of DNA replication. The EMBO Journal 1994, 13: 4145-52. PMID: 8076610, PMCID: PMC395337, DOI: 10.1002/j.1460-2075.1994.tb06732.x.
- Nuclear Targeting of the Parvoviral Replicator Molecule NS1: Evidence for Self-Association Prior to Nuclear TransportNüesch J, Tattersall P. Nuclear Targeting of the Parvoviral Replicator Molecule NS1: Evidence for Self-Association Prior to Nuclear Transport. Virology 1993, 196: 637-651. PMID: 8372437, DOI: 10.1006/viro.1993.1520.
- Asymmetric resolution of a parvovirus palindrome in vitro.Cotmore S, Nüesch J, Tattersall P. Asymmetric resolution of a parvovirus palindrome in vitro. Journal Of Virology 1993, 67: 1579-89. PMID: 8437230, PMCID: PMC237529, DOI: 10.1128/jvi.67.3.1579-1589.1993.
- Expression of functional parvoviral NS1 from recombinant vaccinia virus: Effects of mutations in the nucleotide-binding motifNoesch J, Cotmore S, Tattersall P. Expression of functional parvoviral NS1 from recombinant vaccinia virus: Effects of mutations in the nucleotide-binding motif. Virology 1992, 191: 406-416. PMID: 1413512, DOI: 10.1016/0042-6822(92)90202-z.
- In vitro excision and replication of 5′ telomeres of minute virus of mice DNA from cloned palindromic concatemer junctionsCotmore S, Nuesch J, Tattersall P. In vitro excision and replication of 5′ telomeres of minute virus of mice DNA from cloned palindromic concatemer junctions. Virology 1992, 190: 365-377. PMID: 1388310, DOI: 10.1016/0042-6822(92)91223-h.
- Two amino acid substitutions within the capsid are coordinately required for acquisition of fibrotropism by the lymphotropic strain of minute virus of mice.Ball-Goodrich L, Tattersall P. Two amino acid substitutions within the capsid are coordinately required for acquisition of fibrotropism by the lymphotropic strain of minute virus of mice. Journal Of Virology 1992, 66: 3415-23. PMID: 1316457, PMCID: PMC241122, DOI: 10.1128/jvi.66.6.3415-3423.1992.
- The pathogenesis of infection with minute virus of mice depends on expression of the small nonstructural protein NS2 and on the genotype of the allotropic determinants VP1 and VP2.Brownstein D, Smith A, Johnson E, Pintel D, Naeger L, Tattersall P. The pathogenesis of infection with minute virus of mice depends on expression of the small nonstructural protein NS2 and on the genotype of the allotropic determinants VP1 and VP2. Journal Of Virology 1992, 66: 3118-24. PMID: 1373202, PMCID: PMC241074, DOI: 10.1128/jvi.66.5.3118-3124.1992.
- In vivo resolution of circular plasmids containing concatemer junction fragments from minute virus of mice DNA and their subsequent replication as linear molecules.Cotmore S, Tattersall P. In vivo resolution of circular plasmids containing concatemer junction fragments from minute virus of mice DNA and their subsequent replication as linear molecules. Journal Of Virology 1992, 66: 420-31. PMID: 1530771, PMCID: PMC238302, DOI: 10.1128/jvi.66.1.420-431.1992.
- Pathogenesis of infection with a virulent allotropic variant of minute virus of mice and regulation by host genotype.Brownstein D, Smith A, Jacoby R, Johnson E, Hansen G, Tattersall P. Pathogenesis of infection with a virulent allotropic variant of minute virus of mice and regulation by host genotype. Laboratory Investigation 1991, 65: 357-64. PMID: 1653878.
- Parvoviral target cell specificity: Acquisition of fibrotropism by a mutant of the lymphotropic strain of minute virus of mice involves multiple amino acid substitutions within the capsidBall-Goodrich L, Moir R, Tattersall P. Parvoviral target cell specificity: Acquisition of fibrotropism by a mutant of the lymphotropic strain of minute virus of mice involves multiple amino acid substitutions within the capsid. Virology 1991, 184: 175-186. PMID: 1871965, DOI: 10.1016/0042-6822(91)90834-x.
- Alternate splicing in a parvoviral nonstructural gene links a common amino-terminal sequence to downstream domains which confer radically different localization and turnover characteristicsCotmore S, Tattersall P. Alternate splicing in a parvoviral nonstructural gene links a common amino-terminal sequence to downstream domains which confer radically different localization and turnover characteristics. Virology 1990, 177: 477-487. PMID: 2142555, DOI: 10.1016/0042-6822(90)90512-p.
- Susceptibility of human cells to killing by the parvoviruses H-1 and minute virus of mice correlates with viral transcription.Cornelis J, Chen Y, Spruyt N, Duponchel N, Cotmore S, Tattersall P, Rommelaere J. Susceptibility of human cells to killing by the parvoviruses H-1 and minute virus of mice correlates with viral transcription. Journal Of Virology 1990, 64: 2537-44. PMID: 2139892, PMCID: PMC249429, DOI: 10.1128/jvi.64.6.2537-2544.1990.
- A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles.Cotmore S, Tattersall P. A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles. Journal Of Virology 1989, 63: 3902-11. PMID: 2527311, PMCID: PMC250986, DOI: 10.1128/jvi.63.9.3902-3911.1989.
- Limitations to the expression of parvoviral nonstructural proteins may determine the extent of sensitization of EJ-ras-transformed rat cells to minute virus of miceVan Hille B, Duponchel N, Salomé N, Spruyt N, Cotmore S, Tattersall P, Cornelis J, Rommelaere J. Limitations to the expression of parvoviral nonstructural proteins may determine the extent of sensitization of EJ-ras-transformed rat cells to minute virus of mice. Virology 1989, 171: 89-97. PMID: 2525841, DOI: 10.1016/0042-6822(89)90514-x.
- Evidence for a ligation step in the DNA replication of the autonomous parvovirus minute virus of mice.Cotmore S, Gunther M, Tattersall P. Evidence for a ligation step in the DNA replication of the autonomous parvovirus minute virus of mice. Journal Of Virology 1989, 63: 1002-6. PMID: 2911112, PMCID: PMC247784, DOI: 10.1128/jvi.63.2.1002-1006.1989.
- The terminal protein of minute virus of mice is an 83 kilodalton polypeptide linked to specific forms of double‐stranded and single‐stranded viral DNAGunther M, Tattersall P. The terminal protein of minute virus of mice is an 83 kilodalton polypeptide linked to specific forms of double‐stranded and single‐stranded viral DNA. FEBS Letters 1988, 242: 22-26. PMID: 3203742, DOI: 10.1016/0014-5793(88)80977-3.
- Mapping of the fibrotropic and lymphotropic host range determinants of the parvovirus minute virus of mice.Gardiner E, Tattersall P. Mapping of the fibrotropic and lymphotropic host range determinants of the parvovirus minute virus of mice. Journal Of Virology 1988, 62: 2605-13. PMID: 3392768, PMCID: PMC253690, DOI: 10.1128/jvi.62.8.2605-2613.1988.
- Evidence that developmentally regulated control of gene expression by a parvoviral allotropic determinant is particle mediated.Gardiner E, Tattersall P. Evidence that developmentally regulated control of gene expression by a parvoviral allotropic determinant is particle mediated. Journal Of Virology 1988, 62: 1713-22. PMID: 3357208, PMCID: PMC253210, DOI: 10.1128/jvi.62.5.1713-1722.1988.
- The NS-1 polypeptide of minute virus of mice is covalently attached to the 5' termini of duplex replicative-form DNA and progeny single strands.Cotmore S, Tattersall P. The NS-1 polypeptide of minute virus of mice is covalently attached to the 5' termini of duplex replicative-form DNA and progeny single strands. Journal Of Virology 1988, 62: 851-60. PMID: 3339715, PMCID: PMC253642, DOI: 10.1128/jvi.62.3.851-860.1988.
- Chapter 19 ParvoviridaeCarter B, Tattersall P. Chapter 19 Parvoviridae. 1987, 3: 325-334. DOI: 10.1016/s0168-7069(08)70106-x.
- The Autonomously Replicating Parvoviruses of VertebratesCotmore S, Tattersall P. The Autonomously Replicating Parvoviruses of Vertebrates. 1987, 33: 91-174. PMID: 3296697, DOI: 10.1016/s0065-3527(08)60317-6.
- Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments.Cotmore S, McKie V, Anderson L, Astell C, Tattersall P. Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments. Journal Of Virology 1986, 60: 548-57. PMID: 3021988, PMCID: PMC288924, DOI: 10.1128/jvi.60.2.548-557.1986.
- Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay.Anderson L, Tsou C, Parker R, Chorba T, Wulff H, Tattersall P, Mortimer P. Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay. Journal Of Clinical Microbiology 1986, 24: 522-6. PMID: 3021807, PMCID: PMC268963, DOI: 10.1128/jcm.24.4.522-526.1986.
- The role of parvovirus B19 in aplastic crisis and erythema infectiosum (fifth disease).Chorba T, Coccia P, Holman R, Tattersall P, Anderson L, Sudman J, Young N, Kurczynski E, Saarinen U, Moir R, Lawrence D, Jason J, Evatt B. The role of parvovirus B19 in aplastic crisis and erythema infectiosum (fifth disease). The Journal Of Infectious Diseases 1986, 154: 383-93. PMID: 3016109, DOI: 10.1093/infdis/154.3.383.
- Organization of nonstructural genes of the autonomous parvovirus minute virus of mice.Cotmore S, Tattersall P. Organization of nonstructural genes of the autonomous parvovirus minute virus of mice. Journal Of Virology 1986, 58: 724-32. PMID: 2939261, PMCID: PMC252977, DOI: 10.1128/jvi.58.3.724-732.1986.
- Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis.Shade R, Blundell M, Cotmore S, Tattersall P, Astell C. Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis. Journal Of Virology 1986, 58: 921-36. PMID: 3701931, PMCID: PMC253001, DOI: 10.1128/jvi.58.3.921-936.1986.
- Human parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia.Saarinen U, Chorba T, Tattersall P, Young N, Anderson L, Palmer E, Coccia P. Human parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia. Blood 1986, 67: 1411-7. PMID: 3008891, DOI: 10.1182/blood.v67.5.1411.bloodjournal6751411.
- Human Parvovirus B19-Induced Epidemic Acute Red Cell Aplasia in Patients With Hereditary Hemolytic AnemiaSaarinen U, Chorba T, Tattersall P, Young N, Anderson L, Palmer E, Coccia P. Human Parvovirus B19-Induced Epidemic Acute Red Cell Aplasia in Patients With Hereditary Hemolytic Anemia. Blood 1986, 67: 1411-1417. DOI: 10.1182/blood.v67.5.1411.1411.
- The NS-1 polypeptide of the autonomous parvovirus MVM is a nuclear phosphoproteinCotmore S, Tattersall P. The NS-1 polypeptide of the autonomous parvovirus MVM is a nuclear phosphoprotein. Virus Research 1986, 4: 243-250. PMID: 3739422, DOI: 10.1016/0168-1702(86)90003-1.
- DNA sequence of the lymphotropic variant of minute virus of mice, MVM(i), and comparison with the DNA sequence of the fibrotropic prototype strain.Astell C, Gardiner E, Tattersall P. DNA sequence of the lymphotropic variant of minute virus of mice, MVM(i), and comparison with the DNA sequence of the fibrotropic prototype strain. Journal Of Virology 1986, 57: 656-69. PMID: 3502703, PMCID: PMC252781, DOI: 10.1128/jvi.57.2.656-669.1986.
- CHAPTER 16 THE RODENT PARVOVIRUSESTattersall P, Cotmore S. CHAPTER 16 THE RODENT PARVOVIRUSES. 1986, 305-348. PMCID: PMC7155510, DOI: 10.1016/b978-0-12-095785-9.50023-8.
- Characteristics and Taxonomy of ParvoviridaeSiegl G, Bates R, Berns K, Carter B, Kelly D, Kurstak E, Tattersall P. Characteristics and Taxonomy of Parvoviridae. Intervirology 1985, 23: 61-73. PMID: 3980186, DOI: 10.1159/000149587.
- Characterization and Molecular Cloning of a Human Parvovirus GenomeCotmore S, Tattersall P. Characterization and Molecular Cloning of a Human Parvovirus Genome. Science 1984, 226: 1161-1165. PMID: 6095448, DOI: 10.1126/science.6095448.
- Formation of a host range mutant of the lymphotropic strain of minute virus of mice during persistent infection in mouse L cells.Ron D, Tattersall P, Tal J. Formation of a host range mutant of the lymphotropic strain of minute virus of mice during persistent infection in mouse L cells. Journal Of Virology 1984, 52: 63-9. PMID: 6090711, PMCID: PMC254489, DOI: 10.1128/jvi.52.1.63-69.1984.
- The autonomous parvovirus MVM encodes two nonstructural proteins in addition to its capsid polypeptidesCotmore S, Sturzenbecker L, Tattersall P. The autonomous parvovirus MVM encodes two nonstructural proteins in addition to its capsid polypeptides. Virology 1983, 129: 333-343. PMID: 6623929, DOI: 10.1016/0042-6822(83)90172-1.
- Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice.Merchlinsky M, Tattersall P, Leary J, Cotmore S, Gardiner E, Ward D. Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice. Journal Of Virology 1983, 47: 227-32. PMID: 6345805, PMCID: PMC255236, DOI: 10.1128/jvi.47.1.227-232.1983.
- Interaction of minute virus of mice with differentiated cells: strain-dependent target cell specificity is mediated by intracellular factors.Spalholz B, Tattersall P. Interaction of minute virus of mice with differentiated cells: strain-dependent target cell specificity is mediated by intracellular factors. Journal Of Virology 1983, 46: 937-43. PMID: 6602221, PMCID: PMC256568, DOI: 10.1128/jvi.46.3.937-943.1983.
- Reciprocal productive and restrictive virus-cell interactions of immunosuppressive and prototype strains of minute virus of mice.Tattersall P, Bratton J. Reciprocal productive and restrictive virus-cell interactions of immunosuppressive and prototype strains of minute virus of mice. Journal Of Virology 1983, 46: 944-55. PMID: 6602222, PMCID: PMC256569, DOI: 10.1128/jvi.46.3.944-955.1983.
- Chapter 15 Minute Virus of MiceWard D, Tattersall P. Chapter 15 Minute Virus of Mice. 1982, 313-334. DOI: 10.1016/b978-0-12-262502-2.50022-5.
- About 30% of minute virus of mice RNA is spliced out following polyadenylationTAL J, RON D, TATTERSALL P, BRATOSIN S, ALONI Y. About 30% of minute virus of mice RNA is spliced out following polyadenylation. Nature 1979, 279: 649-651. PMID: 450113, DOI: 10.1038/279649a0.
- Parvoviridae: Second ReportBachmann P, Hoggan D, Kurstak E, Melnick J, Pereira H, Tattersall P, Vago C. Parvoviridae: Second Report. Intervirology 1979, 11: 248-254. PMID: 372134, DOI: 10.1159/000149041.
- Sequence homology between the structural polypeptides of minute virus of miceTattersall P, Shatkin A, Ward D. Sequence homology between the structural polypeptides of minute virus of mice. Journal Of Molecular Biology 1977, 111: 375-394. PMID: 864702, DOI: 10.1016/s0022-2836(77)80060-0.
- Three structural polypeptides coded for by minite virus of mice, a parvovirus.Tattersall P, Cawte P, Shatkin A, Ward D. Three structural polypeptides coded for by minite virus of mice, a parvovirus. Journal Of Virology 1976, 20: 273-89. PMID: 988192, PMCID: PMC354988, DOI: 10.1128/jvi.20.1.273-289.1976.
- Rolling hairpin model for replication of parvovirus and linear chromosomal DNATattersall P, Ward D. Rolling hairpin model for replication of parvovirus and linear chromosomal DNA. Nature 1976, 263: 106-109. PMID: 967244, DOI: 10.1038/263106a0.
- Replication of the parvovirus MVM. II. Isolation and characterization of intermediates in the replication of the viral deoxyribonucleic acid.Tattersall P, Crawford L, Shatkin A. Replication of the parvovirus MVM. II. Isolation and characterization of intermediates in the replication of the viral deoxyribonucleic acid. Journal Of Virology 1973, 12: 1446-56. PMID: 4586779, PMCID: PMC356787, DOI: 10.1128/jvi.12.6.1446-1456.1973.
- Replication of the parvovirus MVM. I. Dependence of virus multiplication and plaque formation on cell growth.Tattersall P. Replication of the parvovirus MVM. I. Dependence of virus multiplication and plaque formation on cell growth. Journal Of Virology 1972, 10: 586-90. PMID: 4673484, PMCID: PMC356507, DOI: 10.1128/jvi.10.4.586-590.1972.