Maura Lane, RVT, RLATG
Research & Publications
Biography
Locations
Research Summary
I am interested in working with and developing new technologies to facilitate our lab's research into congenital malformations. Recently I have used Tol1 and Tol2 transposon technology to create research models with tissue specific fluorescent expression. My current project involves using CRISPR technology to create Knock-in and KO transgenic lines.
Extensive Research Description
I am interested in working with and developing new technologies to facilitate our lab's research into congenital malformations. Recently I have used Tol1 and Tol2 transposon technology to create research models with tissue specific fluorescent expression. My current project involves using CRISPR technology to create Knock-in and KO transgenic lines.
CRISPR technology
Transgenic technology
RNA sequencing expression profiling
Coauthors
Research Interests
Congenital Abnormalities; Animal Diseases; Disease Models, Animal; Infant, Newborn, Diseases; Cardiovascular Abnormalities; Organisms; Diseases
Selected Publications
- Microinjection of Xenopus tropicalis Embryos.Lane M, Mis EK, Khokha MK. Microinjection of Xenopus tropicalis Embryos. Cold Spring Harbor Protocols 2021, 2022: pdb.prot107644. PMID: 34244348, DOI: 10.1101/pdb.prot107644.
- Xenopus Tadpole Craniocardiac Imaging Using Optical Coherence Tomography.Deniz E, Mis EK, Lane M, Khokha MK. Xenopus Tadpole Craniocardiac Imaging Using Optical Coherence Tomography. Cold Spring Harbor Protocols 2021, 2022: pdb.prot105676. PMID: 34031211, DOI: 10.1101/pdb.prot105676.
- Obtaining Xenopus tropicalis Eggs.Lane M, Mis EK, Khokha MK. Obtaining Xenopus tropicalis Eggs. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106344. PMID: 34031209, DOI: 10.1101/pdb.prot106344.
- Raising and Maintaining Xenopus tropicalis from Tadpole to AdultLane M, Slocum M, Khokha MK. Raising and Maintaining Xenopus tropicalis from Tadpole to Adult. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106369. PMID: 34031210, DOI: 10.1101/pdb.prot106369.
- Obtaining Xenopus tropicalis Embryos by In Vitro Fertilization.Lane M, Khokha MK. Obtaining Xenopus tropicalis Embryos by In Vitro Fertilization. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106351. PMID: 34031212, DOI: 10.1101/pdb.prot106351.
- Obtaining Xenopus tropicalis Embryos by Natural Mating.Lane M, Khokha MK. Obtaining Xenopus tropicalis Embryos by Natural Mating. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106609. PMID: 34031213, DOI: 10.1101/pdb.prot106609.
- CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalisDeniz E, Mis EK, Lane M, Khokha MK. CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis. 2018, 1865: 163-174. PMID: 30151766, DOI: 10.1007/978-1-4939-8784-9_12.
- CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editingMoreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ. CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Nature Communications 2017, 8: 2024. PMID: 29222508, PMCID: PMC5722943, DOI: 10.1038/s41467-017-01836-2.
- Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalisLane MA, Kimber M, Khokha MK. Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis. PLOS ONE 2013, 8: e76807. PMID: 24116167, PMCID: PMC3792888, DOI: 10.1371/journal.pone.0076807.
- Xenopus tropicalis genetics identifies chromatoblast stem cell mutantGrammer T, Khokha M, Lane M, Harland R. Xenopus tropicalis genetics identifies chromatoblast stem cell mutant. Developmental Biology 2006, 295: 444-445. DOI: 10.1016/j.ydbio.2006.04.369.
- Identification of mutants in inbred Xenopus tropicalisGrammer TC, Khokha MK, Lane MA, Lam K, Harland RM. Identification of mutants in inbred Xenopus tropicalis. Cells And Development 2005, 122: 263-272. PMID: 15763207, DOI: 10.1016/j.mod.2004.11.003.