Florian Schüder, PhD
Associate Research Scientist in Cell Biology
Research & Publications
Biography
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Research Summary
Florian Schueder is interested in developing novel light microscopy-based super-resolution approaches and applying them to questions in cell biology.
Coauthors
Research Image
Super-resolved DNA origami barcodes
Selected Publications
- Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard.Wickham SFJ, Auer A, Min J, Ponnuswamy N, Woehrstein JB, Schueder F, Strauss MT, Schnitzbauer J, Nathwani B, Zhao Z, Perrault SD, Hahn J, Lee S, Bastings MM, Helmig SW, Kodal AL, Yin P, Jungmann R, Shih WM. Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard. Nature Communications 2020, 11: 5768. PMID: 33188187, PMCID: PMC7666213, DOI: 10.1038/s41467-020-18910-x.
- DNA-Barcoded Fluorescence Microscopy for Spatial Omics.Schueder F, Unterauer EM, Ganji M, Jungmann R. DNA-Barcoded Fluorescence Microscopy for Spatial Omics. Proteomics 2020, e1900368. PMID: 33030780, DOI: 10.1002/pmic.201900368.
- Live-cell super-resolved PAINT imaging of piconewton cellular traction forces.Brockman JM, Su H, Blanchard AT, Duan Y, Meyer T, Quach ME, Glazier R, Bazrafshan A, Bender RL, Kellner AV, Ogasawara H, Ma R, Schueder F, Petrich BG, Jungmann R, Li R, Mattheyses AL, Ke Y, Salaita K. Live-cell super-resolved PAINT imaging of piconewton cellular traction forces. Nature Methods 2020, 17: 1018-1024. PMID: 32929270, PMCID: PMC7574592, DOI: 10.1038/s41592-020-0929-2.
- Super-Resolution Spatial Proximity Detection with Proximity-PAINT.Schueder F, Lara-Gutiérrez J, Haas D, Beckwith KS, Yin P, Ellenberg J, Jungmann R. Super-Resolution Spatial Proximity Detection with Proximity-PAINT. Angewandte Chemie (International Ed. In English) 2020 PMID: 32936507, PMCID: PMC7839522, DOI: 10.1002/anie.202009031.
- Localization microscopy at doubled precision with patterned illumination.Cnossen J, Hinsdale T, Thorsen RØ, Siemons M, Schueder F, Jungmann R, Smith CS, Rieger B, Stallinga S. Localization microscopy at doubled precision with patterned illumination. Nature Methods 2020, 17: 59-63. PMID: 31819263, PMCID: PMC6989044, DOI: 10.1038/s41592-019-0657-7.
- Dynamic host–guest interaction enables autonomous single molecule blinking and super-resolution imagingSasmal R, Das Saha N, Schueder F, Joshi D, Sheeba V, Jungmann R, Agasti SS. Dynamic host–guest interaction enables autonomous single molecule blinking and super-resolution imaging. Chemical Communications 2019, 55: 14430-14433. PMID: 31737873, DOI: 10.1039/c9cc07153a.
- Toward Absolute Molecular Numbers in DNA-PAINT.Stein J, Stehr F, Schueler P, Blumhardt P, Schueder F, Mücksch J, Jungmann R, Schwille P. Toward Absolute Molecular Numbers in DNA-PAINT. Nano Letters 2019, 19: 8182-8190. PMID: 31535868, PMCID: PMC6856960, DOI: 10.1021/acs.nanolett.9b03546.
- An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions.Schueder F, Stein J, Stehr F, Auer A, Sperl B, Strauss MT, Schwille P, Jungmann R. An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions. Nature Methods 2019, 16: 1101-1104. PMID: 31591576, DOI: 10.1038/s41592-019-0584-7.
- Bayesian Multiple Emitter Fitting using Reversible Jump Markov Chain Monte Carlo.Fazel M, Wester MJ, Mazloom-Farsibaf H, Meddens MBM, Eklund AS, Schlichthaerle T, Schueder F, Jungmann R, Lidke KA. Bayesian Multiple Emitter Fitting using Reversible Jump Markov Chain Monte Carlo. Scientific Reports 2019, 9: 13791. PMID: 31551452, PMCID: PMC6760159, DOI: 10.1038/s41598-019-50232-x.
- Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically-Encoded Probes for DNA-PAINT.Schlichthaerle T, Strauss MT, Schueder F, Auer A, Nijmeijer B, Kueblbeck M, Jimenez Sabinina V, Thevathasan JV, Ries J, Ellenberg J, Jungmann R. Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically-Encoded Probes for DNA-PAINT. Angewandte Chemie (International Ed. In English) 2019, 58: 13004-13008. PMID: 31314157, PMCID: PMC6771475, DOI: 10.1002/anie.201905685.
- 124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics.Wade OK, Woehrstein JB, Nickels PC, Strauss S, Stehr F, Stein J, Schueder F, Strauss MT, Ganji M, Schnitzbauer J, Grabmayr H, Yin P, Schwille P, Jungmann R. 124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics. Nano Letters 2019, 19: 2641-2646. PMID: 30864449, PMCID: PMC6463241, DOI: 10.1021/acs.nanolett.9b00508.
- Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification.Stehr F, Stein J, Schueder F, Schwille P, Jungmann R. Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification. Nature Communications 2019, 10: 1268. PMID: 30894522, PMCID: PMC6426843, DOI: 10.1038/s41467-019-09064-6.
- Nanometer-scale Multiplexed Super-Resolution Imaging with an Economic 3D-DNA-PAINT Microscope.Auer A, Schlichthaerle T, Woehrstein JB, Schueder F, Strauss MT, Grabmayr H, Jungmann R. Nanometer-scale Multiplexed Super-Resolution Imaging with an Economic 3D-DNA-PAINT Microscope. Chemphyschem : A European Journal Of Chemical Physics And Physical Chemistry 2018, 19: 3024-3034. PMID: 30207624, DOI: 10.1002/cphc.201800630.
- Organellar Proteomics and Phospho-Proteomics Reveal Subcellular Reorganization in Diet-Induced Hepatic Steatosis.Krahmer N, Najafi B, Schueder F, Quagliarini F, Steger M, Seitz S, Kasper R, Salinas F, Cox J, Uhlenhaut NH, Walther TC, Jungmann R, Zeigerer A, Borner GHH, Mann M. Organellar Proteomics and Phospho-Proteomics Reveal Subcellular Reorganization in Diet-Induced Hepatic Steatosis. Developmental Cell 2018, 47: 205-221.e7. PMID: 30352176, DOI: 10.1016/j.devcel.2018.09.017.
- Template-free 2D particle fusion in localization microscopy.Heydarian H, Schueder F, Strauss MT, van Werkhoven B, Fazel M, Lidke KA, Jungmann R, Stallinga S, Rieger B. Template-free 2D particle fusion in localization microscopy. Nature Methods 2018, 15: 781-784. PMID: 30224671, DOI: 10.1038/s41592-018-0136-6.
- Site-Specific Labeling of Affimers for DNA-PAINT Microscopy.Schlichthaerle T, Eklund AS, Schueder F, Strauss MT, Tiede C, Curd A, Ries J, Peckham M, Tomlinson DC, Jungmann R. Site-Specific Labeling of Affimers for DNA-PAINT Microscopy. Angewandte Chemie (International Ed. In English) 2018, 57: 11060-11063. PMID: 29873161, DOI: 10.1002/anie.201804020.
- Quantifying absolute addressability in DNA origami with molecular resolution.Strauss MT, Schueder F, Haas D, Nickels PC, Jungmann R. Quantifying absolute addressability in DNA origami with molecular resolution. Nature Communications 2018, 9: 1600. PMID: 29686288, PMCID: PMC5913233, DOI: 10.1038/s41467-018-04031-z.
- Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT.Schueder F, Lara-Gutiérrez J, Beliveau BJ, Saka SK, Sasaki HM, Woehrstein JB, Strauss MT, Grabmayr H, Yin P, Jungmann R. Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT. Nature Communications 2017, 8: 2090. PMID: 29233999, PMCID: PMC5727263, DOI: 10.1038/s41467-017-02028-8.
- Programmable self-assembly of three-dimensional nanostructures from 10,000 unique components.Ong LL, Hanikel N, Yaghi OK, Grun C, Strauss MT, Bron P, Lai-Kee-Him J, Schueder F, Wang B, Wang P, Kishi JY, Myhrvold C, Zhu A, Jungmann R, Bellot G, Ke Y, Yin P. Programmable self-assembly of three-dimensional nanostructures from 10,000 unique components. Nature 2017, 552: 72-77. PMID: 29219968, PMCID: PMC5786436, DOI: 10.1038/nature24648.
- Super-resolution microscopy with DNA-PAINT.Schnitzbauer J, Strauss MT, Schlichthaerle T, Schueder F, Jungmann R. Super-resolution microscopy with DNA-PAINT. Nature Protocols 2017, 12: 1198-1228. PMID: 28518172, DOI: 10.1038/nprot.2017.024.
- DNA-barcoded labeling probes for highly multiplexed Exchange-PAINT imaging.Agasti SS, Wang Y, Schueder F, Sukumar A, Jungmann R, Yin P. DNA-barcoded labeling probes for highly multiplexed Exchange-PAINT imaging. Chemical Science (Royal Society Of Chemistry : 2010) 2017, 8: 3080-3091. PMID: 28451377, PMCID: PMC5380918, DOI: 10.1039/c6sc05420j.
- Universal Super-Resolution Multiplexing by DNA Exchange.Schueder F, Strauss MT, Hoerl D, Schnitzbauer J, Schlichthaerle T, Strauss S, Yin P, Harz H, Leonhardt H, Jungmann R. Universal Super-Resolution Multiplexing by DNA Exchange. Angewandte Chemie (International Ed. In English) 2017, 56: 4052-4055. PMID: 28256790, PMCID: PMC5540260, DOI: 10.1002/anie.201611729.
- DNA nanotechnology and fluorescence applications.Schlichthaerle T, Strauss MT, Schueder F, Woehrstein JB, Jungmann R. DNA nanotechnology and fluorescence applications. Current Opinion In Biotechnology 2016, 39: 41-47. PMID: 26773303, DOI: 10.1016/j.copbio.2015.12.014.
- Routing of individual polymers in designed patterns.Knudsen JB, Liu L, Bank Kodal AL, Madsen M, Li Q, Song J, Woehrstein JB, Wickham SF, Strauss MT, Schueder F, Vinther J, Krissanaprasit A, Gudnason D, Smith AA, Ogaki R, Zelikin AN, Besenbacher F, Birkedal V, Yin P, Shih WM, Jungmann R, Dong M, Gothelf KV. Routing of individual polymers in designed patterns. Nature Nanotechnology 2015, 10: 892-8. PMID: 26322946, DOI: 10.1038/nnano.2015.190.
- Anle138b and related compounds are aggregation specific fluorescence markers and reveal high affinity binding to α-synuclein aggregates.Deeg AA, Reiner AM, Schmidt F, Schueder F, Ryazanov S, Ruf VC, Giller K, Becker S, Leonov A, Griesinger C, Giese A, Zinth W. Anle138b and related compounds are aggregation specific fluorescence markers and reveal high affinity binding to α-synuclein aggregates. Biochimica Et Biophysica Acta 2015, 1850: 1884-90. PMID: 26028294, DOI: 10.1016/j.bbagen.2015.05.021.
- DNA-Based Self-Assembly of Fluorescent Nanodiamonds.Zhang T, Neumann A, Lindlau J, Wu Y, Pramanik G, Naydenov B, Jelezko F, Schüder F, Huber S, Huber M, Stehr F, Högele A, Weil T, Liedl T. DNA-Based Self-Assembly of Fluorescent Nanodiamonds. Journal Of The American Chemical Society 2015, 137: 9776-9. PMID: 26196373, DOI: 10.1021/jacs.5b04857.