2000
Nicotine Binding to Native and Substituted Peptides Comprising Residues 188–207 of Nicotinic Acetylcholine Receptor α1, α2, α3, α4, α5, and α7 Subunits
Lentz T. Nicotine Binding to Native and Substituted Peptides Comprising Residues 188–207 of Nicotinic Acetylcholine Receptor α1, α2, α3, α4, α5, and α7 Subunits. Biochemical And Biophysical Research Communications 2000, 268: 480-484. PMID: 10679230, DOI: 10.1006/bbrc.2000.2155.Peer-Reviewed Original ResearchConceptsNicotinic acetylcholine receptor α1Acetylcholine receptor alpha subunitNicotinic acetylcholine receptor alpha-subunitHigh affinity binding componentDifferent alpha subunitsReceptor alpha subunitNeuronal peptidesReceptor α1Alpha subunitΑ7 subunitAlpha4 subunitSignificant decreaseNicotineBinding componentSynthetic peptidesLow affinityPeptidesHigh affinity
1996
Rabies virus binding to the nicotinic acetylcholine receptor α subunit demonstrated by virus overlay protein binding assay
Gastka M, Horvath J, Lentz T. Rabies virus binding to the nicotinic acetylcholine receptor α subunit demonstrated by virus overlay protein binding assay. Journal Of General Virology 1996, 77: 2437-2440. PMID: 8887475, DOI: 10.1099/0022-1317-77-10-2437.Peer-Reviewed Original ResearchConceptsAlpha subunitNicotinic acetylcholine receptor α subunitAcetylcholine receptor α subunitElectric organ membranesVirus overlay proteinVirus overlay protein binding assaysTransfer of proteinsReceptor α subunitProtein binding assaysAChR alpha subunitOverlay proteinRabies virusΑ-subunitSubunitsGel electrophoresisAcetylcholine receptorsBinding assaysCuraremimetic neurotoxinsProteinMembraneVirusBindingAChRBlot
1993
Effects of mutations of Torpedo acetylcholine receptor alpha 1 subunit residues 184-200 on alpha-bungarotoxin binding in a recombinant fusion protein.
Chaturvedi V, Donnelly-Roberts D, Lentz T. Effects of mutations of Torpedo acetylcholine receptor alpha 1 subunit residues 184-200 on alpha-bungarotoxin binding in a recombinant fusion protein. Biochemistry 1993, 32: 9570-6. PMID: 8373764, DOI: 10.1021/bi00088a008.Peer-Reviewed Original ResearchConceptsAlpha 1 subunitFusion proteinAlpha-bungarotoxin bindingFusion protein containingOligonucleotide-directed mutagenesisEffects of mutationsPossible structure-function relationshipsStructure-function relationshipsTyr-189Dissimilar residuesPro-194Recombinant fusion proteinHigher apparent affinityAlpha subunitTyr-198Asp-195Cys-192Cys-193Protein containingFunctional interactionAmino acidsSubunitsResiduesMutationsPosition 184
1992
Substitution of Torpedo acetylcholine receptor alpha 1-subunit residues with snake alpha 1- and rat nerve alpha 3-subunit residues in recombinant fusion proteins: effect on alpha-bungarotoxin binding.
Chaturvedi V, Donnelly-Roberts D, Lentz T. Substitution of Torpedo acetylcholine receptor alpha 1-subunit residues with snake alpha 1- and rat nerve alpha 3-subunit residues in recombinant fusion proteins: effect on alpha-bungarotoxin binding. Biochemistry 1992, 31: 1370-5. PMID: 1736994, DOI: 10.1021/bi00120a012.Peer-Reviewed Original Research
1987
Synthetic peptides corresponding to sequences of snake venom neurotoxins and rabies virus glycoprotein bind to the nicotinic acetylcholine receptor
Lentz T, Hawrot E, Wilson P. Synthetic peptides corresponding to sequences of snake venom neurotoxins and rabies virus glycoprotein bind to the nicotinic acetylcholine receptor. Proteins Structure Function And Bioinformatics 1987, 2: 298-307. PMID: 3448605, DOI: 10.1002/prot.340020406.Peer-Reviewed Original ResearchConceptsLoop 2Alpha-bungarotoxin bindingAcetylcholine receptorsProtein-protein interactionsElectric organ acetylcholine receptorAlpha-subunit peptidesApparent affinityVirus glycoproteinCompetitive antagonist d-tubocurarineRabies virus glycoproteinSynthetic peptidesSnake venom neurotoxinsViral homologsAlpha subunitNative proteinNicotinic acetylcholine receptorsGlycoprotein actsGlu residuesIntact subunitVenom neurotoxinsHydrophobic subsitePosition 173Recognition sitesAntagonist d-tubocurarineArg37
1985
Determination of the primary amino acid sequence specifying the alpha-bungarotoxin binding site on the alpha subunit of the acetylcholine receptor from Torpedo californica.
Wilson P, Lentz T, Hawrot E. Determination of the primary amino acid sequence specifying the alpha-bungarotoxin binding site on the alpha subunit of the acetylcholine receptor from Torpedo californica. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 8790-8794. PMID: 3866252, PMCID: PMC391523, DOI: 10.1073/pnas.82.24.8790.Peer-Reviewed Original ResearchConceptsBungarotoxin-binding sitesPrimary amino acid sequenceAmino acid sequenceAlpha subunitAmino acidsAcid sequenceAcetylcholine receptorsNicotinic acetylcholine receptorsAlpha-bungarotoxin binding sitesEndoglycosidase H.Residues 173Torpedo californicaV8 proteaseSubunitsOligosaccharide chainsProteolytic fragmentsBinding sitesPresumed siteSize of fragmentsFragmentsSynthetic peptidesSequenceBind 125IDigestionMajor determinant
1983
Binding of alpha-bungarotoxin to isolated alpha subunit of the acetylcholine receptor of Torpedo californica: quantitative analysis with protein blots.
Gershoni J, Hawrot E, Lentz T. Binding of alpha-bungarotoxin to isolated alpha subunit of the acetylcholine receptor of Torpedo californica: quantitative analysis with protein blots. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 4973-4977. PMID: 6576369, PMCID: PMC384170, DOI: 10.1073/pnas.80.16.4973.Peer-Reviewed Original ResearchConceptsAlpha subunitProtein blotsEndoglycosidase HHigh-mannose oligosaccharide chainsAcetylcholine receptorsAssociation of toxinAcetylcholine receptor subunitsReceptor-containing membranesOligosaccharide sideTorpedo electric organSingle labeled bandDirect bindingTorpedo acetylcholine receptorTorpedo californicaSubunits