2011
AGS-3 Alters Caenorhabditis elegans Behavior after Food Deprivation via RIC-8 Activation of the Neural G Protein Gαo
Hofler C, Koelle MR. AGS-3 Alters Caenorhabditis elegans Behavior after Food Deprivation via RIC-8 Activation of the Neural G Protein Gαo. Journal Of Neuroscience 2011, 31: 11553-11562. PMID: 21832186, PMCID: PMC3161416, DOI: 10.1523/jneurosci.2072-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCaenorhabditis elegans ProteinsCells, CulturedDrosophilaFood DeprivationGTP-Binding Protein alpha Subunits, Gi-GoGuanine Nucleotide Dissociation InhibitorsGuanine Nucleotide Exchange FactorsHumansNeuronsNuclear ProteinsProtein BindingSignal TransductionConceptsAGS-3GPR proteinsG proteinsGenetic epistasis experimentsG protein GαoRegulator domainGPR domainEpistasis experimentsBiochemical fractionationChemosensory neuronsBiological functionsBiological roleFood deprivationProteinCaenorhabditisDependent fashionFood-deprived animalsActivationVivoGαoGTPDomainAnimalsNeuronsDeprivation
2010
A Conserved Protein Interaction Interface on the Type 5 G Protein β Subunit Controls Proteolytic Stability and Activity of R7 Family Regulator of G Protein Signaling Proteins*
Porter MY, Xie K, Pozharski E, Koelle MR, Martemyanov KA. A Conserved Protein Interaction Interface on the Type 5 G Protein β Subunit Controls Proteolytic Stability and Activity of R7 Family Regulator of G Protein Signaling Proteins*. Journal Of Biological Chemistry 2010, 285: 41100-41112. PMID: 20959458, PMCID: PMC3003408, DOI: 10.1074/jbc.m110.163600.Peer-Reviewed Original ResearchConceptsR7 RGS proteinsG protein signaling (RGS) proteinsRGS proteinsDEP domainSignaling proteinsProtein interaction interfacesGenetic screenCaenorhabditis elegansRGS complexesObligate complexesProtein complexesFamily regulatorGβ5 proteinEquivalent mutationN-terminusConformational rearrangementsGβ5ProteinInteraction interfaceProteolysisMutationsRegulatorProteolytic stabilityComplexesDynamic opening
2009
Chapter 2 Insights into RGS Protein Function from Studies in Caenorhabditis elegans
Porter MY, Koelle MR. Chapter 2 Insights into RGS Protein Function from Studies in Caenorhabditis elegans. Progress In Nucleic Acid Research And Molecular Biology 2009, 86: 15-47. PMID: 20374712, DOI: 10.1016/s1877-1173(09)86002-x.Peer-Reviewed Original ResearchConceptsRGS proteinsGα proteinsMultiple RGS proteinsRGS protein functionG protein αRGS domainCaenorhabditis elegansProtein subfamiliesC. elegansGα genesProtein functionChapter 2 InsightsVivo functionNematode wormsPhysiological functionsProtein αProteinElegansSubfamiliesSuch specificityDifferent cellsRGSOrthologsCaenorhabditisMammals
2007
Biogenic amine neurotransmitters in C. elegans.
Chase DL, Koelle MR. Biogenic amine neurotransmitters in C. elegans. WormBook 2007, 1-15. PMID: 18050501, PMCID: PMC4781333, DOI: 10.1895/wormbook.1.132.1.Peer-Reviewed Original Research
2005
Caenorhabditus elegans Arrestin Regulates Neural G Protein Signaling and Olfactory Adaptation and Recovery*
Palmitessa A, Hess HA, Bany IA, Kim YM, Koelle MR, Benovic JL. Caenorhabditus elegans Arrestin Regulates Neural G Protein Signaling and Olfactory Adaptation and Recovery*. Journal Of Biological Chemistry 2005, 280: 24649-24662. PMID: 15878875, DOI: 10.1074/jbc.m502637200.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAmino Acid SequenceAnimalsAnimals, Genetically ModifiedArrestinBenzaldehydesBlotting, NorthernCaenorhabditis elegansCell LineCells, CulturedChemotaxisClathrinCOS CellsDiacetylEndocytosisExonsGreen Fluorescent ProteinsGTP-Binding ProteinsHumansImmunohistochemistryModels, GeneticMolecular Sequence DataMutationNeuronsOdorantsOlfactory PathwaysPentanolsPhenotypePhylogenyProtein BindingProtein Structure, TertiarySequence Analysis, DNASignal TransductionTime FactorsConceptsARR-1Receptor endocytosisG protein signalingG protein-coupled receptorsOlfactory adaptationVolatile odorantsProtein-coupled receptorsPotential mechanistic basisEndocytic machineryCaenorhabditis elegansNull mutantsHSN neuronsProtein signalingReceptor kinaseAdaptation defectRecovery defectArrestin functionChemosensory neuronsEnvironmental cuesBind proteinsMechanistic basisVivo linkTransgenic expressionArrestinNormal chemotaxis
2004
Domains, Amino Acid Residues, and New Isoforms of Caenorhabditis elegans Diacylglycerol Kinase 1 (DGK-1) Important for Terminating Diacylglycerol Signaling in Vivo *
Jose AM, Koelle MR. Domains, Amino Acid Residues, and New Isoforms of Caenorhabditis elegans Diacylglycerol Kinase 1 (DGK-1) Important for Terminating Diacylglycerol Signaling in Vivo *. Journal Of Biological Chemistry 2004, 280: 2730-2736. PMID: 15563467, PMCID: PMC2048986, DOI: 10.1074/jbc.m409460200.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAlternative SplicingAmino Acid SequenceAnimalsBase SequenceBinding SitesCaenorhabditis elegansCell LineCodonCodon, TerminatorDiacylglycerol KinaseDiglyceridesExonsHumansInsectaModels, GeneticMolecular Sequence DataMutationPhosphorylationPlasmidsProtein IsoformsProtein Structure, TertiaryRecombinant ProteinsSequence Homology, Amino AcidSignal TransductionConceptsCysteine-rich domainAmino acid residuesDGK-1Pleckstrin homology domainKinase domainDiacylglycerol kinaseAmino acid substitutionsAcid residuesHomology domainATP-binding site mutationsStop codonSecond cysteine-rich domainPhysiological functionsAcid substitutionsThird cysteine-rich domainHuman diacylglycerol kinaseNovel splice formsSubstituted amino acid residuesDiacylglycerol signalingPremature stop codonCaenorhabditis elegansSplice formsStop codon mutantKey residuesNew isoformGenetic Analysis of RGS Protein Function in Caenorhabditis elegans
Chase DL, Koelle MR. Genetic Analysis of RGS Protein Function in Caenorhabditis elegans. Methods In Enzymology 2004, 389: 305-320. PMID: 15313573, DOI: 10.1016/s0076-6879(04)89018-9.Peer-Reviewed Original ResearchConceptsRGS proteinsC. elegansG proteinsRGS protein functionStructure/function studiesG protein geneCaenorhabditis elegansGalpha mutantsClose homologProtein functionGalpha proteinsElegansGenetic analysisDetailed protocolTransgenic expressionProteinMost mammaliansMutantsFunction studiesOrthologsCaenorhabditisHomologMammalianGenesOrganisms
1997
A new family of G-protein regulators — the RGS proteins
Koelle M. A new family of G-protein regulators — the RGS proteins. Current Opinion In Cell Biology 1997, 9: 143-147. PMID: 9069252, DOI: 10.1016/s0955-0674(97)80055-5.Peer-Reviewed Original ResearchConceptsRGS proteinsInactive GDP-bound formG protein alpha subunitsG proteinsDifferent RGS proteinsHeterotrimeric G proteinsG-protein regulatorsGDP-bound formG protein signalingFirst biochemical studyGenetic experimentsGTPase activityAlpha subunitBiochemical studiesProteinPhysiological significanceRegulatorFamilySubunitsSignalingNew familyLarge number