Makoto Sahara, MD, PhD, FAHA
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About
Research
Publications
2024
Author Correction: Placental growth factor exerts a dual function for cardiomyogenesis and vasculogenesis during heart development
Witman N, Zhou C, Häneke T, Xiao Y, Huang X, Rohner E, Sohlmér J, Grote Beverborg N, Lehtinen M, Chien K, Sahara M. Author Correction: Placental growth factor exerts a dual function for cardiomyogenesis and vasculogenesis during heart development. Nature Communications 2024, 15: 283. PMID: 38177121, PMCID: PMC10766948, DOI: 10.1038/s41467-023-44507-1.Peer-Reviewed Original Research
2023
STRA6 is essential for induction of vascular smooth muscle lineages in human embryonic cardiac outflow tract development
Zhou C, Häneke T, Rohner E, Sohlmér J, Kameneva P, Artemov A, Adameyko I, Sahara M. STRA6 is essential for induction of vascular smooth muscle lineages in human embryonic cardiac outflow tract development. Cardiovascular Research 2023, 119: 1202-1217. PMID: 36635482, PMCID: PMC10202647, DOI: 10.1093/cvr/cvad010.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsHuman cardiogenesisWild-type human embryonic stem cellsSmooth muscle cellsCardiac outflow tract developmentSingle-cell RNA sequencing datasetsRARα/RXRαCardiogenic transcription factorsRNA sequencing datasetsSmooth muscle lineageRetinoic acid signalingEmbryonic stem cellsVascular smooth muscle lineageRNA-seq dataOutflow tract developmentCardiac outflow tractOFT formationHeart progenitorsMuscle lineageSTRA6 mutationsRas signalingMurine embryonic heartTranscription factorsAcid signalingHeart development
2022
Progress in Bioengineering Strategies for Heart Regenerative Medicine
Häneke T, Sahara M. Progress in Bioengineering Strategies for Heart Regenerative Medicine. International Journal Of Molecular Sciences 2022, 23: 3482. PMID: 35408844, PMCID: PMC8998628, DOI: 10.3390/ijms23073482.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHuman pluripotent stem cellsCardiac tissue engineering strategiesRegenerative medicineStem cell engineeringHeart regenerationNovel functional biomaterialsCardiac tissue engineeringStem cell biologyTissue engineering strategiesHeart diseasePluripotent stem cellsEngineering strategiesCell engineeringFunctional biomaterialsTissue engineeringCardiac biologyCell biologyBioengineering strategiesGreat promiseCardiomyocyte maturationDisease modelingHeart regenerative medicineInsufficient therapeutic optionsStem cellsTherapeutic applicationsUncovering the molecular identity of cardiosphere-derived cells (CDCs) by single-cell RNA sequencing
Kogan P, Wirth F, Tomar A, Darr J, Teperino R, Lahm H, Dreßen M, Puluca N, Zhang Z, Neb I, Beck N, Luzius T, de la Osa de la Rosa L, Gärtner K, Hüls C, Zeidler R, Ramanujam D, Engelhardt S, Wenk C, Holdt L, Mononen M, Sahara M, Cleuziou J, Hörer J, Lange R, Krane M, Doppler S. Uncovering the molecular identity of cardiosphere-derived cells (CDCs) by single-cell RNA sequencing. Basic Research In Cardiology 2022, 117: 11. PMID: 35258704, PMCID: PMC8902493, DOI: 10.1007/s00395-022-00913-y.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingExtracellular vesiclesRNA sequencingMolecular identityCell typesMitochondria-rich cell typesCardiosphere-derived cellsMitochondria-rich cellsHuman-induced pluripotent stem cellsPluripotent stem cellsCardiac progenitor cellsPro-apoptotic BaxGO termsNon-hematopoietic cellsCardiac developmentTranscriptomic similarityStem cellsProgenitor cellsCellular originNon-myocyte cellsNew specific markerCulture conditionsBiological similaritiesSpecial culture conditionsSequencing
2021
An mRNA assay system demonstrates proteasomal-specific degradation contributes to cardiomyopathic phospholamban null mutation
Rohner E, Witman N, Sohlmer J, De Genst E, Louch W, Sahara M, Chien K. An mRNA assay system demonstrates proteasomal-specific degradation contributes to cardiomyopathic phospholamban null mutation. Molecular Medicine 2021, 27: 102. PMID: 34496741, PMCID: PMC8425124, DOI: 10.1186/s10020-021-00362-8.Peer-Reviewed Original ResearchConceptsNull mutationMolecular pathwaysProtein analysis toolsBulk RNA sequencingRNA sequencing analysisWild-type reporterDetailed molecular pathwaysDistinct transcriptomic signaturesTranscriptional profilingRNA sequencingProteasomal pathwayHuman embryonic stem cell-derived cardiomyocytesEmbryonic stem cell-derived cardiomyocytesProtein expression levelsProteasomal inhibitionChemical inhibitorsDetectable proteinTranscriptomic signaturesSequencing analysisDegradation contributesMRNA constructsStem cell-derived cardiomyocytesPLN proteinExpression levelsDegradation pathwayIsolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis
Santoro F, Chien K, Sahara M. Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis. STAR Protocols 2021, 2: 100339. PMID: 33644774, PMCID: PMC7887647, DOI: 10.1016/j.xpro.2021.100339.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cell differentiationEmbryonic stem cell differentiationSingle-cell RNA-seq analysisSingle-cell RNA sequencing analysisComprehensive transcriptional analysisRNA-seq analysisStem cell differentiationRNA sequencing analysisCardiac derivativesFluorescence-activated cell sortingSmart-seq2Developmental tissuesTranscriptional analysisCombination of populationCDNA libraryMolecular atlasHuman embryogenesisHuman ESCsCell differentiationSequencing analysisComplete detailsCell sortingPowerful approachEmbryonic heart cellsDifferentiation
2020
Corrigendum to “Cardiac progenitors and paracrine mediators in cardiogenesis and heart regeneration” [Semin. Cell Dev. Biol. Vol.100 (2020) 29–51]
Witman N, Zhou C, Beverborg N, Sahara M, Chien K. Corrigendum to “Cardiac progenitors and paracrine mediators in cardiogenesis and heart regeneration” [Semin. Cell Dev. Biol. Vol.100 (2020) 29–51]. Seminars In Cell And Developmental Biology 2020, 109: 151. PMID: 33039299, DOI: 10.1016/j.semcdb.2020.09.008.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsGenome-wide CRISPR screen identifies ZIC2 as an essential gene that controls the cell fate of early mesodermal precursors to human heart progenitors
Xu J, Zhou C, Foo K, Yang R, Xiao Y, Bylund K, Sahara M, Chien K. Genome-wide CRISPR screen identifies ZIC2 as an essential gene that controls the cell fate of early mesodermal precursors to human heart progenitors. Stem Cells 2020, 38: 741-755. PMID: 32129551, PMCID: PMC7891398, DOI: 10.1002/stem.3168.Peer-Reviewed Original ResearchConceptsHuman pluripotent stem cellsCRISPR knockout screensCell fateProgenitor formationEssential genesHuman cardiogenesisGenome-wide CRISPR knockout screenProgenitor cell fate determinationEarly mesodermal precursorsSingle-cell RNA-seq analysisMesoderm precursor cellsCell fate determinationDevelopmental signaling cascadesProgenitor cell fateRNA-seq analysisRNA-seq profilingMultiple gene setsPluripotent stem cellsMesoderm formationMesodermal precursorsHeart progenitorsCommitted stepMesodermal formationGene setsMaster regulator
2019
Cardiac progenitors and paracrine mediators in cardiogenesis and heart regeneration
Witman N, Zhou C, Grote Beverborg N, Sahara M, Chien K. Cardiac progenitors and paracrine mediators in cardiogenesis and heart regeneration. Seminars In Cell And Developmental Biology 2019, 100: 29-51. PMID: 31862220, DOI: 10.1016/j.semcdb.2019.10.011.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsCardiac progenitorsHeart regenerationCardiac progenitor cell biologyMammalian cardiac developmentProgenitor cell biologyParacrine mediatorsMorphogenetic processesCell biologyCardiac developmentMolecular mechanismsCell-free therapyCardiogenesisModern biotechnologyDiseased settingsMammalian heartProgenitorsCongenital heart diseaseRegenerative capabilityTissue engineering platformsRecent knowledgeEngineering platformHeart diseaseNovel toolHeart repairRegenerationIn search of the next super models
Goedel A, Beverborg N, Sahara M, Chien K. In search of the next super models. EMBO Molecular Medicine 2019, 11: e11502. PMID: 31736275, PMCID: PMC6895600, DOI: 10.15252/emmm.201911502.Commentaries, Editorials and Letters