Junichi Saito, PhD, MD
About
Research
Publications
2024
Endothelial HIFα-PDGF-B to smooth muscle Beclin1 signaling sustains pathological muscularization in pulmonary hypertension
Saddouk F, Kuzemczak A, Saito J, Greif D. Endothelial HIFα-PDGF-B to smooth muscle Beclin1 signaling sustains pathological muscularization in pulmonary hypertension. JCI Insight 2024, 9: e162449. PMID: 38652543, PMCID: PMC11141934, DOI: 10.1172/jci.insight.162449.Peer-Reviewed Original ResearchSmooth muscle cellsArteriole smooth muscle cellsPulmonary hypertensionPlatelet-derived growth factor-BDistal muscularizationSugen 5416Endothelial cellsHuman idiopathic pulmonary arterial hypertensionHypoxia-induced pulmonary vascular remodelingPulmonary artery smooth muscle cellsIdiopathic pulmonary arterial hypertensionHypoxia-inducible factor (HIF)-1aArtery smooth muscle cellsDistal pulmonary arteriolesPulmonary arterial hypertensionPulmonary vascular remodelingDeletion of Hif1aLung endothelial cellsGrowth factor BEC-specific deletionPulmonary arteriolesArterial hypertensionLung lysatesMuscle cellsVascular remodeling
2023
Presenilin-1 in smooth muscle cells facilitates hypermuscularization in elastin aortopathy
Saito J, Dave J, Lau F, Greif D. Presenilin-1 in smooth muscle cells facilitates hypermuscularization in elastin aortopathy. IScience 2023, 27: 108636. PMID: 38226162, PMCID: PMC10788461, DOI: 10.1016/j.isci.2023.108636.Peer-Reviewed Original ResearchHydrostatic pressure under hypoxia facilitates fabrication of tissue-engineered vascular grafts derived from human vascular smooth muscle cells in vitro.
Kojima T, Nakamura T, Saito J, Hidaka Y, Akimoto T, Inoue H, Chick CN, Usuki T, Kaneko M, Miyagi E, Ishikawa Y, Yokoyama U. Hydrostatic pressure under hypoxia facilitates fabrication of tissue-engineered vascular grafts derived from human vascular smooth muscle cells in vitro. Acta Biomater 2023, 171: 209-222. PMID: 37793599, DOI: 10.1016/j.actbio.2023.09.041.Peer-Reviewed Original ResearchMolecular mechanisms regulating extracellular matrix-mediated remodeling in the ductus arteriosus.
Yokoyama U, Oka S, Saito J. Molecular mechanisms regulating extracellular matrix-mediated remodeling in the ductus arteriosus. Semin Perinatol 2023, 47: 151716. PMID: 36906477, DOI: 10.1016/j.semperi.2023.151716.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2022
JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency
Dave JM, Chakraborty R, Ntokou A, Saito J, Saddouk FZ, Feng Z, Misra A, Tellides G, Riemer RK, Urban Z, Kinnear C, Ellis J, Mital S, Mecham R, Martin KA, Greif DM. JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency. Journal Of Clinical Investigation 2022, 132: e142338. PMID: 34990407, PMCID: PMC8884911, DOI: 10.1172/jci142338.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsSupravalvular aortic stenosisEndothelial cellsElastin insufficiencyObstructive arterial diseaseAortic smooth muscle cellsΓ-secretaseAortic vascular cellsPotential therapeutic targetNotch3 intracellular domainNotch ligand Jagged1Aortic stenosisArterial diseasePathological featuresPharmacological treatmentJag1 deletionLuminal obstructionMouse modelNotch3 activationTherapeutic targetSMC accumulationPathway upregulationAortic samplesMice displayNotch3 deletion
2021
Scaffold-free tissue-engineered arterial grafts derived from human skeletal myoblasts.
Saito J, Yokoyama U, Nakamura T, Kanaya T, Ueno T, Naito Y, Takayama T, Kaneko M, Miyagawa S, Sawa Y, Ishikawa Y. Scaffold-free tissue-engineered arterial grafts derived from human skeletal myoblasts. Artif Organs 2021, 45: 919-932. PMID: 33539557, DOI: 10.1111/aor.13930.Peer-Reviewed Original ResearchTranscriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans.
Saito J, Kojima T, Tanifuji S, Kato Y, Oka S, Ichikawa Y, Miyagi E, Tachibana T, Asou T, Yokoyama U. Transcriptome Analysis Reveals Differential Gene Expression between the Closing Ductus Arteriosus and the Patent Ductus Arteriosus in Humans. J Cardiovasc Dev Dis 2021, 8 PMID: 33923468, DOI: 10.3390/jcdd8040045.Peer-Reviewed Original ResearchChallenges and Possibilities of Cell-Based Tissue-Engineered Vascular Grafts.
Saito J, Kaneko M, Ishikawa Y, Yokoyama U. Challenges and Possibilities of Cell-Based Tissue-Engineered Vascular Grafts. Cyborg Bionic Syst 2021, 2021: 1532103. PMID: 36285145, DOI: 10.34133/2021/1532103.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2020
Fibulin-1 Integrates Subendothelial Extracellular Matrices and Contributes to Anatomical Closure of the Ductus Arteriosus.
Ito S, Yokoyama U, Nakakoji T, Cooley MA, Sasaki T, Hatano S, Kato Y, Saito J, Nicho N, Iwasaki S, Umemura M, Fujita T, Masuda M, Asou T, Ishikawa Y. Fibulin-1 Integrates Subendothelial Extracellular Matrices and Contributes to Anatomical Closure of the Ductus Arteriosus. Arterioscler Thromb Vasc Biol 2020, 40: 2212-2226. PMID: 32640908, DOI: 10.1161/ATVBAHA.120.314729.Peer-Reviewed Original ResearchRole of Tissue-Type Plasminogen Activator in Remodeling of the Ductus Arteriosus.
Saito J, Ishikawa Y, Yokoyama U. Role of Tissue-Type Plasminogen Activator in Remodeling of the Ductus Arteriosus. Circ Rep 2020, 2: 211-217. PMID: 33693232, DOI: 10.1253/circrep.CR-20-0015.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements