Joanna Pawlak, PhD, MSc
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About
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Associate Research Scientist
Education & Training
- PhD
- Leiden University (2017)
- MSc
- University of Amsterdam (2012)
Research
Research at a Glance
Yale Co-Authors
Frequent collaborators of Joanna Pawlak's published research.
Publications Timeline
A big-picture view of Joanna Pawlak's research output by year.
Maudry Laurent-Rolle, MD, PhD, BS
Craig B. Wilen, MD, PhD
Peter Cresswell, PhD, FRS
10Publications
293Citations
Publications
2024
Comprehensive Assessment of Inactivation Methods for Madariaga Virus
Boytz R, Keita K, Pawlak J, Laurent-Rolle M. Comprehensive Assessment of Inactivation Methods for Madariaga Virus. Viruses 2024, 16: 206. PMID: 38399982, PMCID: PMC10892135, DOI: 10.3390/v16020206.Peer-Reviewed Original ResearchMeSH Keywords and ConceptsConceptsEastern equine encephalitis virusMadariaga virusMethod of inactivationInactivation methodSelective agentNorth American strainsEEEV strainsEquine encephalitis virusTRIzol LSAmino acid levelsAmerican strainsEncephalitis virusAcid levelsTRIzolInfected cellsVirusFormalinInactivationPathogensPublic health threatInfected supernatantStrain
2023
CMPK2 restricts Zika virus replication by inhibiting viral translation
Pawlak J, Hsu J, Xia H, Han P, Suh H, Grove T, Morrison J, Shi P, Cresswell P, Laurent-Rolle M. CMPK2 restricts Zika virus replication by inhibiting viral translation. PLOS Pathogens 2023, 19: e1011286. PMID: 37075076, PMCID: PMC10150978, DOI: 10.1371/journal.ppat.1011286.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsCytidine/uridine monophosphate kinase 2I interferon-stimulated genesZika virus replicationYellow fever virusAntiviral activityAntiviral effectVirus replicationKunjin virusType I interferon-stimulated genesFirst lineOverall antiviral responseHost's first lineEffective therapeutic interventionsViral translationBroad antiviral activityInterferon-stimulated genesGlobal health threatAntiviral treatmentFlaviviral infectionsPathogenic flavivirusesAntiviral functionDrug AdministrationTherapeutic interventionsAntiviral responseDengue virus
2022
Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling
Hsu JC, Pawlak JB, Laurent-Rolle M, Cresswell P. Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling. STAR Protocols 2022, 3: 101654. PMID: 36072758, PMCID: PMC9442383, DOI: 10.1016/j.xpro.2022.101654.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsTranslational regulationMammalian cell linesDifferent cell typesDifferent growth conditionsMammalian cellsTranslation activityGene expressionBiological processesCell typesComplete detailsConfocal microscopyCell linesGrowth conditionsPuromycin analoguesCritical roleRegulationCellsProteinLabelingFundamental stepExpressionClick chemistry reactionActivityCytometryViperin triggers ribosome collision-dependent translation inhibition to restrict viral replication
Hsu JC, Laurent-Rolle M, Pawlak JB, Xia H, Kunte A, Hee JS, Lim J, Harris LD, Wood JM, Evans GB, Shi PY, Grove TL, Almo SC, Cresswell P. Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication. Molecular Cell 2022, 82: 1631-1642.e6. PMID: 35316659, PMCID: PMC9081181, DOI: 10.1016/j.molcel.2022.02.031.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsInterferon-stimulated genesS-adenosyl methionineTranslation inhibitionRadical S-adenosyl methionineInnate immune responseIntegrated stress response pathwayStress response pathwaysViral RNA translationImmune responseAntiviral responseInnate immunityAntiviral mechanismTranslation regulatorsTranslational repressionViral replicationEnzymatic productDidehydro-CTPResponse pathwaysRNA translationViperinSAM activityPathwayInhibitionBroad spectrumReplication
2021
Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein
Hsu JC, Laurent-Rolle M, Pawlak JB, Wilen CB, Cresswell P. Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2101161118. PMID: 34045361, PMCID: PMC8214666, DOI: 10.1073/pnas.2101161118.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsSARS-CoV-2Interferon-stimulated genesImmune responseSevere acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2Host protein synthesisRespiratory syndrome coronavirus 2Syndrome coronavirus 2Innate immune responseUnprecedented global health crisisCoronavirus 2N7-methyltransferase activityOngoing COVID-19 pandemicHuman coronavirusesTranslational shutdownVirus replicationNsp14 proteinGlobal health crisisProtein synthesisInhibition activityCausative agentCOVID-19COVID-19 pandemicSARS-CoV-2 nsp14Dependent induction
2018
Chemical Control over T‑Cell Activation in Vivo Using Deprotection of trans-Cyclooctene-Modified Epitopes
van der Gracht A, de Geus M, Camps M, Ruckwardt T, Sarris A, Bremmers J, Maurits E, Pawlak J, Posthoorn M, Bonger K, Filippov D, Overkleeft H, Robillard M, Ossendorp F, van Kasteren S. Chemical Control over T‑Cell Activation in Vivo Using Deprotection of trans-Cyclooctene-Modified Epitopes. ACS Chemical Biology 2018, 13: 1569-1576. PMID: 29733186, PMCID: PMC6006443, DOI: 10.1021/acschembio.8b00155.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and Concepts
2017
Correlative light and electron microscopy reveals discrepancy between gold and fluorescence labelling
VAN ELSLAND D, BOS E, PAWLAK J, OVERKLEEFT H, KOSTER A, VAN KASTEREN S. Correlative light and electron microscopy reveals discrepancy between gold and fluorescence labelling. Journal Of Microscopy 2017, 267: 309-317. PMID: 28470743, DOI: 10.1111/jmi.12567.Peer-Reviewed Original ResearchCitationsMeSH Keywords and Concepts
2016
The Optimization of Bioorthogonal Epitope Ligation within MHC‑I Complexes
Pawlak J, Hos B, van de Graaff M, Megantari O, Meeuwenoord N, Overkleeft H, Filippov D, Ossendorp F, van Kasteren S. The Optimization of Bioorthogonal Epitope Ligation within MHC‑I Complexes. ACS Chemical Biology 2016, 11: 3172-3178. PMID: 27704768, DOI: 10.1021/acschembio.6b00498.Peer-Reviewed Original ResearchCitationsMeSH Keywords and Concepts
2015
Bioorthogonal Deprotection on the Dendritic Cell Surface for Chemical Control of Antigen Cross‐Presentation
Pawlak J, Gential G, Ruckwardt T, Bremmers J, Meeuwenoord N, Ossendorp F, Overkleeft H, Filippov D, van Kasteren S. Bioorthogonal Deprotection on the Dendritic Cell Surface for Chemical Control of Antigen Cross‐Presentation. Angewandte Chemie 2015, 127: 5720-5723. DOI: 10.1002/ange.201500301.Peer-Reviewed Original ResearchCitationsBioorthogonal Deprotection on the Dendritic Cell Surface for Chemical Control of Antigen Cross‐Presentation
Pawlak J, Gential G, Ruckwardt T, Bremmers J, Meeuwenoord N, Ossendorp F, Overkleeft H, Filippov D, van Kasteren S. Bioorthogonal Deprotection on the Dendritic Cell Surface for Chemical Control of Antigen Cross‐Presentation. Angewandte Chemie International Edition 2015, 54: 5628-5631. PMID: 25784151, DOI: 10.1002/anie.201500301.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and Concepts
Academic Achievements and Community Involvement
honor TL1
Yale School of Medicine AwardYale Center for Clinical InvestigationDetails07/01/2023United Stateshonor Ruth L. Kirschstein
National AwardNational Institutes of HealthDetails01/01/2021United Stateshonor Rubicon
International AwardNetherlands Organization for Scientific Research (NWO)Details10/01/2018Netherlands
Links & Media
News
- June 28, 2023
“Coolest Protein” Restricts Zika Virus Replication
- July 15, 2021
Discoveries & Impact (July 2021)