Chin-Yi Chen
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Research
Publications
2020
Three-Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons Using Selective Plane Illumination
Wu F, Pratiwi F, Chen C, Lu C, Tang W, Liu Y, Chen B, Chen P. Three-Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons Using Selective Plane Illumination. Neuromethods 2020, 154: 261-293. DOI: 10.1007/978-1-0716-0532-5_13.Peer-Reviewed Original ResearchSuper-resolution imagingSuper-resolution optical microscopy techniquesThree-dimensional imagingThree-dimensional super-resolution imagingSelective plane illumination microscopySelective plane illuminationSuper-resolution microscopeThree-Dimensional SuperOptical microscopy techniquesLong data acquisitionBessel beamsSuper-resolution fluorescence microscopyPlane illuminationOptical sectioningFast data acquisitionResolution imagingPhotodamage effectsLarge fieldIllumination microscopyMicroscopy techniquesIllumination techniqueActivation sourceData acquisitionDetail structureLimited fieldAurora B functions at the apical surface after specialized cytokinesis during morphogenesis in C. elegans
Bai X, Melesse M, Turpin C, Sloan DE, Chen CY, Wang WC, Lee PY, Simmons JR, Nebenfuehr B, Mitchell D, Klebanow LR, Mattson N, Betzig E, Chen BC, Cheerambathur D, Bembenek JN. Aurora B functions at the apical surface after specialized cytokinesis during morphogenesis in C. elegans. Development 2020, 147: dev181099. PMID: 31806662, PMCID: PMC6983721, DOI: 10.1242/dev.181099.Peer-Reviewed Original ResearchConceptsAspects of cytokinesisAurora B functionAurora B kinaseApical surfaceC. elegansEpithelial polarizationEmbryonic divisionsAurora BB kinaseCytokinesisApical structuresSpecific regulationB functionsTissue typesMorphogenesisEpithelial cellsEarly divisionRing componentsUnexpected alterationsSensory neuronsSpecialized wayCaenorhabditisElegansCellsDivisionThree-Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons Using Selective Plane Illumination
Frances Camille M. Wu, Feby Wijaya Pratiwi, Chin-Yi Chen, Chieh-Han Lu, Wei-Chun Tang, Yen-Ting Liu, Bi-Chang Chen, Peilin Chen Single Molecule Microscopy in Neurobiology, Neuromethods, volume 154 Humana, New York, NYBooks
2019
The Applications of Lattice Light-sheet Microscopy for Functional Volumetric Imaging of Hippocampal Neurons in a Three-Dimensional Culture System
Chen CY, Liu YT, Lu CH, Lee PY, Tsai YC, Wu JS, Chen P, Chen BC. The Applications of Lattice Light-sheet Microscopy for Functional Volumetric Imaging of Hippocampal Neurons in a Three-Dimensional Culture System. Micromachines 2019, 10: 599. PMID: 31514427, PMCID: PMC6780203, DOI: 10.3390/mi10090599.Peer-Reviewed Original ResearchTissue engineering techniquesAxial directionMicrofluidic systemVoltage responseImage pixel sizePixel sizeVolumetric imagesThick samplesThree-dimensional cell cultureHigh spatiotemporal resolutionEngineering techniquesThree-dimensionsConventional microscopeMicroscopyLaser exposureSpatiotemporal resolutionLightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging
Lu CH, Tang WC, Liu YT, Chang SW, Wu FCM, Chen CY, Tsai YC, Yang SM, Kuo CW, Okada Y, Hwu YK, Chen P, Chen BC. Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging. Communications Biology 2019, 2: 177. PMID: 31098410, PMCID: PMC6509110, DOI: 10.1038/s42003-019-0403-9.Peer-Reviewed Original Research
2018
Programmed Variations of Cytokinesis Contribute to Morphogenesis in the C. elegans embryo
Xiaofei Bai, Po-Yi Lee, Chin-Yi Chen, James R. Simmons, Benjamin Nebenfuehr, Diana Mitchell, Lindsey R. Klebanow, Nicholas Mattson, Christopher Turpin, Bi-Chang Chen, Eric Betzig, Joshua N. Bembenek bioRxiv doi: https://doi.org/10.1101/319657Peer-Reviewed Case Reports and Technical Notes
2016
Postsynaptic Y654 dephosphorylation of β‐catenin modulates presynaptic vesicle turnover through increased n‐cadherin‐mediated transsynaptic signaling
Chen C, Chen Y, Wang J, Huang Y, Tai C. Postsynaptic Y654 dephosphorylation of β‐catenin modulates presynaptic vesicle turnover through increased n‐cadherin‐mediated transsynaptic signaling. Developmental Neurobiology 2016, 77: 61-74. PMID: 27328456, DOI: 10.1002/dneu.22411.Peer-Reviewed Original ResearchConceptsN-cadherinPhosphorylation-defective formSynaptic vesicle proteinsSynaptic adhesion moleculesSynaptic vesicle turnoverRecycling vesicle poolRetrograde signalingScaffold proteinHeterophilic adhesionVesicle proteinsSynaptotagmin-1Vesicle dynamicsVesicle turnoverVesicle poolSynaptic vesiclesPresynaptic vesicle dynamicsMature neuronsTranssynaptic communicationPostsynaptic expressionSynaptic communicationAdhesion moleculesProteinSynaptic plasticityEnhanced bindingPresynaptic terminalsPRRT2 mutations lead to neuronal dysfunction and neurodevelopmental defects
Liu YT, Nian FS, Chou WJ, Tai CY, Kwan SY, Chen C, Kuo PW, Lin PH, Chen CY, Huang CW, Lee YC, Soong BW, Tsai JW. PRRT2 mutations lead to neuronal dysfunction and neurodevelopmental defects. Oncotarget 2016, 7: 39184-39196. PMID: 27172900, PMCID: PMC5129924, DOI: 10.18632/oncotarget.9258.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChlorocebus aethiopsCOS CellsCytoplasmDisease Models, AnimalDystoniaEpilepsyGenetic Predisposition to DiseaseHEK293 CellsHeterozygoteHippocampusHumansIntellectual DisabilityMembrane ProteinsMiceMice, Inbred ICRMutationMutation, MissenseNerve Tissue ProteinsNeurodegenerative DiseasesNeuronsRatsRats, Sprague-DawleyRNA, Small InterferingTaiwanConceptsDisease-causing mechanismsParoxysmal kinesigenic dyskinesiaProtein 2 geneMissense mutantsProline-rich transmembrane protein 2 (PRRT2) geneEmbryonic developmentMembrane fractionationPost-synaptic membraneSevere clinical symptomsNeurodevelopmental defectsPRRT2 mutationsPRRT2 expressionNovel disease-causing mechanismsCell membraneNeuronal migrationMutationsProtein expressionMutantsUtero electroporationClinical symptomsNeuronal dysfunctionSynaptic densityCortical neuronsFunction effectsPathologic effects
2012
The protective roles of phosphorylated heat shock protein 27 in human cells harboring myoclonus epilepsy with ragged‐red fibers A8344G mtDNA mutation
Chen H, Chen C, Lin T, Huang Z, Chi T, Ma Y, Wu S, Wei Y, Hsieh M. The protective roles of phosphorylated heat shock protein 27 in human cells harboring myoclonus epilepsy with ragged‐red fibers A8344G mtDNA mutation. The FEBS Journal 2012, 279: 2987-3001. PMID: 22742457, DOI: 10.1111/j.1742-4658.2012.08678.x.Peer-Reviewed Original ResearchConceptsMutant cybridsA8344G mutationHeat shock protein 27Shock protein 27Mitochondrial diseaseMERRF syndromeWild-type cybridsMitochondrial protein synthesisHeat shock stressProtein 27Phosphorylated heat shock protein 27P-HSP27Mitochondrial DNA mutationsG mutationRagged-red fibers (MERRF) syndromeHsp27 mutantsNormal kinaseSpecific serineCytoplasmic hybridsMaternal lineagesSteady-state levelsNormal phosphorylationApoptotic stressCybridsMtDNA mutations
2011
Decreased heat shock protein 27 expression and altered autophagy in human cells harboring A8344G mitochondrial DNA mutation
Chen CY, Chen HF, Gi SJ, Chi TH, Cheng CK, Hsu CF, Ma YS, Wei YH, Liu CS, Hsieh M. Decreased heat shock protein 27 expression and altered autophagy in human cells harboring A8344G mitochondrial DNA mutation. Mitochondrion 2011, 11: 739-749. PMID: 21679777, DOI: 10.1016/j.mito.2011.05.014.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisAutophagyAutophagy-Related Protein 12Autophagy-Related Protein 5Caspase 3Cells, CulturedDNA, MitochondrialDown-RegulationEnzyme ActivationHeat-Shock ProteinsHSP27 Heat-Shock ProteinsHSP70 Heat-Shock ProteinsHSP90 Heat-Shock ProteinsHumansMERRF SyndromeMicrotubule-Associated ProteinsMolecular ChaperonesPoint MutationProteolysisSmall Ubiquitin-Related Modifier ProteinsStress, PhysiologicalConceptsMERRF cybridsA8344G mutationAutophagic pathwayMitochondrial DNA mutationsMutant cybridsDNA mutationsMERRF syndromeNormal heat shock responseNormal cytoplasmic distributionWild-type HSP27G mutationHeat shock responseA8344G mitochondrial DNA mutationElectron transport chainMERRF cellsCybrid cellsHeat shock protein 27Accumulation of HSP27Impairs assemblyHeat shock protein 27 expressionShock protein 27CybridsStaurosporine treatmentWild typeCytoplasmic distribution