Chikondi Peno
Postdoctoral AssociateAbout
Research
Publications
2023
2604. Contact With School-Aged Children is a Major Risk Factor for Pneumococcal Colonization in Older Adults
Wyllie A, Yolda-Carr D, Hislop M, Mbodj S, Kennedy H, McLaughlin M, Wurst L, Waghela P, Peno C, Alexander-Parrish R, Arguedas A, Gessner B, Weinberger D. 2604. Contact With School-Aged Children is a Major Risk Factor for Pneumococcal Colonization in Older Adults. Open Forum Infectious Diseases 2023, 10: ofad500.2218. PMCID: PMC10679235, DOI: 10.1093/ofid/ofad500.2218.Peer-Reviewed Original ResearchPneumococcal conjugate vaccineOlder adultsTime pointsRisk factorsPneumococcal colonizationAdult transmissionTransmission of pneumococciMajor risk factorRecent contactYear oldsNon-institutionalized individualsYears of ageGrant/research supportSame time pointsPneumococcal acquisitionConjugate vaccineSchool-Aged ChildrenElevated prevalenceYoung school-age childrenHealth statusYoung contactsSaliva samplesMedical directorsSample adultsPneumococci881. Saliva-Based, Extraction-Free PCR Testing For The Detection Of Key Respiratory Pathogens
Allicock O, Lin T, Fajardo K, Yolda-Carr D, Hislop M, Wang J, Zuniga D, Platt W, Tuohy B, Peno C, Wyllie A. 881. Saliva-Based, Extraction-Free PCR Testing For The Detection Of Key Respiratory Pathogens. Open Forum Infectious Diseases 2023, 10: ofad500.926. PMCID: PMC10677551, DOI: 10.1093/ofid/ofad500.926.Peer-Reviewed Original ResearchSARS-CoV-2Saliva samplesRespiratory virusesRespiratory pathogensSARS-CoV-2 PCR testInfluenza A/BSARS-CoV-2 testingUpper respiratory tract pathogensAdditional respiratory virusesDetection of hMPVMass testing strategyPCR testCommon respiratory virusesCommon respiratory pathogensRespiratory tract pathogensKey respiratory pathogensYears of ageSARS-CoV-2 detectionGrant/research supportTransmissible infectious diseasesUse of salivaYale HealthRespiratory symptomsInfluenza B.Nasopharyngeal swabsThe potential of saliva as an accessible and sensitive sample type for the detection of respiratory pathogens and host immunity
Laxton C, Peno C, Hahn A, Allicock O, Perniciaro S, Wyllie A. The potential of saliva as an accessible and sensitive sample type for the detection of respiratory pathogens and host immunity. The Lancet Microbe 2023, 4: e837-e850. PMID: 37516121, DOI: 10.1016/s2666-5247(23)00135-0.Peer-Reviewed Original ResearchConceptsSARS-CoV-2Respiratory pathogensPotential of salivaUsefulness of salivaUse of salivaRespiratory specimenRespiratory secretionsDisease burdenImmunological componentsImmunity surveillanceClinical utilityHost immunityClinical useDiagnostic testsNon-invasive natureSalivaCOVID-19 pandemicSample typesMethodological factorsPathogensImmunitySecretionStreptococcus pyogenes Colonization in Children Aged 24–59 Months in the Gambia: Impact of Live Attenuated Influenza Vaccine and Associated Serological Responses
Keeley A, Groves D, Armitage E, Senghore E, Jagne Y, Sallah H, Drammeh S, Angyal A, Hornsby H, de Crombrugghe G, Smeesters P, Rossi O, Carducci M, Peno C, Bogaert D, Kampmann B, Marks M, Shaw H, Turner C, de Silva T. Streptococcus pyogenes Colonization in Children Aged 24–59 Months in the Gambia: Impact of Live Attenuated Influenza Vaccine and Associated Serological Responses. The Journal Of Infectious Diseases 2023, 228: 957-965. PMID: 37246259, PMCID: PMC10547459, DOI: 10.1093/infdis/jiad153.Peer-Reviewed Original ResearchConceptsQuantitative polymerase chain reactionLAIV groupInfluenza vaccineSerological responseOdds ratioDay 7Live attenuated influenza vaccineChildren Aged 24High-burden settingsAttenuated influenza vaccineControl group participantsBurden settingsGambian childrenNasopharyngeal swabsPoint prevalenceAged 24Polymerase chain reactionAsymptomatic colonizationNasopharyngeal colonizationIgG increaseControl groupDay 21Day 0LAIVS. pyogenesRoutine saliva testing for SARS-CoV-2 in children: Methods for partnering with community childcare centers
Rayack E, Askari H, Zirinsky E, Lapidus S, Sheikha H, Peno C, Kazemi Y, Yolda-Carr D, Liu C, Grubaugh N, Ko A, Wyllie A, Spatz E, Oliveira C, Bei A. Routine saliva testing for SARS-CoV-2 in children: Methods for partnering with community childcare centers. Frontiers In Public Health 2023, 11: 1003158. PMID: 36817891, PMCID: PMC9936085, DOI: 10.3389/fpubh.2023.1003158.Peer-Reviewed Original ResearchConceptsParents/guardiansOnline patient portalYounger age groupsSARS-CoV-2Age groupsSurveillance programSaliva collectionSARS-CoV-2 testingSARS-CoV-2 screeningWeekly saliva samplesRT-PCR testingChildcare centre staffCritical age groupRoutine surveillance toolRoutine testing programsChildcare centersCOVID-19 transmissionAsymptomatic screeningSaliva collection methodNasal swabsPatient portalsSymptomatic testingPublic health dataSaliva samplesChildcare facilities
2022
Effect of intra-partum azithromycin on the development of the infant nasopharyngeal microbiota: A post hoc analysis of a double-blind randomized trial
Sanyang B, de Silva T, Kanteh A, Bojang A, Manneh J, de Steenhuijsen Piters W, Peno C, Bogaert D, Sesay A, Roca A. Effect of intra-partum azithromycin on the development of the infant nasopharyngeal microbiota: A post hoc analysis of a double-blind randomized trial. EBioMedicine 2022, 83: 104227. PMID: 35988464, PMCID: PMC9420482, DOI: 10.1016/j.ebiom.2022.104227.Peer-Reviewed Original ResearchConceptsNasopharyngeal microbiotaDay 6Neonatal periodDouble-blind randomized placebo-controlled clinical trialRandomized placebo-controlled clinical trialPlacebo-controlled clinical trialEarly antibiotic exposureMonths of ageMedical Research CouncilOverall microbiota compositionOral azithromycinPlacebo armNasopharyngeal carriageHealthy infantsModest overall effectNeonatal deathNasopharyngeal samplesMicrobiota perturbationsClinical trialsLeading causeAntibiotic exposureBacterial carriageShort-term alterationsMicrobiota compositionAzithromycin
2021
The effect of live attenuated influenza vaccine on pneumococcal colonisation densities among children aged 24–59 months in The Gambia: a phase 4, open label, randomised, controlled trial
Peno C, Armitage E, Clerc M, Balcazar Lopez C, Jagne Y, Drammeh S, Jarju S, Sallah H, Senghore E, Lindsey B, Camara J, Bah S, Mohammed N, Dockrell D, Kampmann B, Clarke E, Bogaert D, de Silva T. The effect of live attenuated influenza vaccine on pneumococcal colonisation densities among children aged 24–59 months in The Gambia: a phase 4, open label, randomised, controlled trial. The Lancet Microbe 2021, 2: e656-e665. PMID: 34881370, PMCID: PMC8632704, DOI: 10.1016/s2666-5247(21)00179-8.Peer-Reviewed Original ResearchConceptsPneumococcal densityRespiratory viral infectionsLAIV groupMiddle-income country populationsPneumococcal carriageRespiratory virusesControl groupDay 7Influenza vaccineViral infectionDay 21Asymptomatic respiratory viral infectionsHigher pneumococcal carriage ratesHigh pneumococcal carriageInfluenza vaccine programsNasopharyngeal pneumococcal carriagePneumococcal carriage rateUse of LAIVAttenuated influenza vaccineUpper respiratory tractInvestigator teamOpen labelRandomisation sequencePrimary outcomeCarriage prevalenceGenomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa
Cornick J, Musicha P, Peno C, Seager E, Tam P, Bilima S, Bennett A, Kennedy N, Feasey N, Heinz E, Cain A. Genomic investigation of a suspected Klebsiella pneumoniae outbreak in a neonatal care unit in sub-Saharan Africa. Microbial Genomics 2021, 7: 000703. PMID: 34793293, PMCID: PMC8743538, DOI: 10.1099/mgen.0.000703.Peer-Reviewed Original ResearchConceptsStrain sequence typesBloodstream infectionsSpecial Care Neonatal UnitNeonatal care unitImportant hospital pathogensKlebsiella pneumoniae outbreakLarge public hospitalCare unitNeonatal unitPediatric wardNeonatal wardHospital outbreaksTreat infectionsHospital pathogensPublic hospitalsPlasmid typesHospitalWhole-genome sequencingInfectionSequence typesWardsYear periodSaharan AfricaOutbreakLikely causeWhole genome sequence analysis of Shigella from Malawi identifies fluoroquinolone resistance
Stenhouse G, Jere K, Peno C, Bengtsson R, Chinyama E, Mandolo J, Cain A, Iturriza-Gómara M, Bar-Zeev N, Cunliffe N, Cornick J, Baker K. Whole genome sequence analysis of Shigella from Malawi identifies fluoroquinolone resistance. Microbial Genomics 2021, 7: 000532. PMID: 33945457, PMCID: PMC8209728, DOI: 10.1099/mgen.0.000532.Peer-Reviewed Original Research
2020
Bacterial genome-wide association study of hyper-virulent pneumococcal serotype 1 identifies genetic variation associated with neurotropism
Chaguza C, Yang M, Cornick JE, du Plessis M, Gladstone RA, Kwambana-Adams BA, Lo SW, Ebruke C, Tonkin-Hill G, Peno C, Senghore M, Obaro SK, Ousmane S, Pluschke G, Collard JM, Sigaùque B, French N, Klugman KP, Heyderman RS, McGee L, Antonio M, Breiman RF, von Gottberg A, Everett DB, Kadioglu A, Bentley SD. Bacterial genome-wide association study of hyper-virulent pneumococcal serotype 1 identifies genetic variation associated with neurotropism. Communications Biology 2020, 3: 559. PMID: 33033372, PMCID: PMC7545184, DOI: 10.1038/s42003-020-01290-9.Peer-Reviewed Original ResearchConceptsGenome-wide association studiesPneumococcal serotype 1Genetic variationBacterial genome-wide association studiesAssociation studiesSerotype 1Central nervous system infectionSignificant genotype-phenotype associationsNervous system infectionGenotype-phenotype associationsHelicase proteinPopulation structureCNS infectionsSystem infectionNegligible heritabilityMeningitis outbreakCNS tissueCerebrospinal fluidImmune evasionSerotype 1 strainPneumococcal survivalPolygenic etiologySmall effect sizesMeningitisTropism