2024
A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva.
Peno C, Lin T, Hislop M, Yolda-Carr D, Farjado K, York A, Pitzer V, Weinberger D, Bei A, Allicock O, Wyllie A. A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva. Microbiology Spectrum 2024, e0059124. PMID: 39028185, DOI: 10.1128/spectrum.00591-24.Peer-Reviewed Original ResearchDetection of pneumococciDetection of pneumococcal carriagePneumococcal carriageCarriage surveillanceLow-resource settingsChildren attending childcare centersCarriage of pneumococciDNA extractionSaliva samplesMolecular methodsCultural enrichmentImprove surveillance effortsQPCR-based protocolPneumococcal vaccineExtraction-free methodMolecular detectionNucleic acid extractionVaccination strategiesPneumococciCulture-enrichment methodExtraction-free protocolPurified DNASalivaPaired samplesCarriage
2016
Molecular surveillance of nasopharyngeal carriage of Streptococcus pneumoniae in children vaccinated with conjugated polysaccharide pneumococcal vaccines
Wyllie AL, Wijmenga-Monsuur AJ, van Houten MA, Bosch AATM, Groot JA, van Engelsdorp Gastelaars J, Bruin JP, Bogaert D, Rots NY, Sanders EAM, Trzciński K. Molecular surveillance of nasopharyngeal carriage of Streptococcus pneumoniae in children vaccinated with conjugated polysaccharide pneumococcal vaccines. Scientific Reports 2016, 6: 23809. PMID: 27046258, PMCID: PMC4820691, DOI: 10.1038/srep23809.Peer-Reviewed Original ResearchConceptsPneumococcal conjugate vaccinePolysaccharide pneumococcal vaccineVaccine-targeted serotypesStreptococcus pneumoniae carriageCross-sectional surveillanceVaccine serotypes 4Number of serotypesPneumococcal surveillancePneumococcal vaccinePneumoniae carriageUnvaccinated individualsConjugate vaccineNasopharyngeal carriagePneumococcal diseaseVaccine effectsNasopharyngeal samplesConventional diagnostic methodsSurveillance studyS. pneumoniaeStreptococcus pneumoniaeSerotype detectionMolecular surveillanceHidden circulationSerotypesDiagnostic methods