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Bioinformatics workshop series

Session 4: RNAseq Data Processing: A Step-by-Step Guide from raw sequences to counts

Register: https://schedule.yale.edu/event/11967121


Presenter: Dr. Francesc Lopez-Giraldez, Yale Center for Genomic Analysis

Summary: Learn the detailed process of transforming raw RNAseq data (fastq format) into meaningful raw counts, without relying on automated pipelines. This hands-on workshop emphasizes the step-by-step methodology for data processing and analysis. For this hands-on workshop, participants will be using Open OnDemand and Jupyter Notebooks.

You will learn how to:

  • QC, filter, and trim NGS sequences
  • Align sequences to a reference genome
  • Produce a count matrix ready to be used with DESeq2

Requirements:

  • Have participated to session 3 or being familiar with bash/CLI or Jupyter Notebook
  • Bring a laptop
  • HPC account to access Yale Open OnDemand, if you don't have an account bring your laptop with R and RStudio installed.

This session is suitable for both beginner and intermediate users.

Speaker

Admission

Free

Tag

Training