Robert Tigelaar, MD
Research & Publications
Biography
Research Summary
Dr. Robert Tigelaar's major research focus continues to be the dendritic epidermal d T cells (DETC) populating the skin of all normal strains of mice. A number of longstanding collaborations between the Tigelaar lab and those of Michael Girardi (Dermatology) and Adrian Hayday (Guy’s Immunobiology [London]) include:
- The generation and characterization of a Vg5-/- “knockout” mouse selectively deficient in DETCs expressing the TCR prototypically seen on >95% of such cells; studies of such mice unexpectedly showed that in the absence of Vg5, the replacement population of d DETC includes a significant number of cells expressing a Vd1/d1 TCR with a similar 3-D conformation as the conventional Vg5/Vd1 TCR (as defined by an anti-clonotypic antibody to the Vg5/Vd1 TCR);
- Documentation that some (e.g., FVB and NOD), but not other (B6) strains of mice genetically deficient in d T cells (d-/- mice) develop a localized (ears) cutaneous inflammation/ spontaneous dermatitis (SpD) that is dependent (like atopic dermatitis) both upon conventional aß T cells and upon an appropriate external environment.
Adoptive transfer studies showed that Vg5+ DETC but not systemic Vg5- d cells were necessary and sufficient to down-regulate SpD. Finally, crosses of susceptible (NOD) and resistant (C57BL/6) d-/- mice which showed that susceptibility to SpD behaves as a recessive trait, have been recently analyzed by genome-wide, microsatellite mapping; these studies clearly indicate that several distinct genetic intervals contribute to the regulation of this cutaneous inflammatory response. Ongoing studies in this arena:
- To characterize in more detail the pathology in SpD normally down-regulated by skin-associated DETC;
- To characterize the genes expressed by “resting” DETC and in vitro “activated” DETC by serial analysis of gene expression (SAGE);
- To investigate the potentials of selected candidate DETC cytokines/effector molecules to down-regulate SpD.
Specialized Terms: Immunobiology of gdT cells; Immune system-skin interactions; Immunopathogenesis of contact dermatitis, atopic dermatitis, and cutaneous T-cell lymphoma
Extensive Research Description
Dr. Robert Tigelaar's major research focus continues to be the dendritic epidermal d T cells (DETC) populating the skin of all normal strains of mice. A number of longstanding collaborations between the Tigelaar lab and those of Michael Girardi (Dermatology) and Adrian Hayday (Guy’s Immunobiology [London]) include:
- The generation and characterization of a Vg5-/- “knockout” mouse selectively deficient in DETCs expressing the TCR prototypically seen on >95% of such cells; studies of such mice unexpectedly showed that in the absence of Vg5, the replacement population of d DETC includes a significant number of cells expressing a Vd1/d1 TCR with a similar 3-D conformation as the conventional Vg5/Vd1 TCR (as defined by an anti-clonotypic antibody to the Vg5/Vd1 TCR)
- Documentation that some (e.g., FVB and NOD), but not other (B6) strains of mice genetically deficient in d T cells (d-/- mice) develop a localized (ears) cutaneous inflammation/ spontaneous dermatitis (SpD) that is dependent (like atopic dermatitis) both upon conventional aß T cells and upon an appropriate external environment.
Adoptive transfer studies showed that Vg5+ DETC but not systemic Vg5- d cells were necessary and sufficient to down-regulate SpD. Finally, crosses of susceptible (NOD) and resistant (C57BL/6) d-/- mice which showed that susceptibility to SpD behaves as a recessive trait, have been recently analyzed by genome-wide, microsatellite mapping; these studies clearly indicate that several distinct genetic intervals contribute to the regulation of this cutaneous inflammatory response. Ongoing studies in this arena:
- To characterize in more detail the pathology in SpD normally down-regulated by skin-associated DETC
- To characterize the genes expressed by “resting” DETC and in vitro “activated” DETC by serial analysis of gene expression (SAGE)
- To investigate the potentials of selected candidate DETC cytokines/effector molecules to down-regulate SpD
Recent studies of a spontaneous mutation arising in a substrain of FVB mice that results in a striking deficiency in the skin of the prototypic Vg5/Vd1+ DETCs seen in other normal mice strains led to further studies that proved that this heritable defect in a dominant gene resided in fetal thymic epithelial cells resulting in a failure of positive selection in the thymus (and subsequent migration to the skin) of the Vg5/Vd1+ fetal thymic precursors. (These results, published in Nature Immunology, present the first definitive proof that d cells resident in epithelial interfaces with the external environment, like conventional recirculating aß T cells, undergo positive selection in the thymus.) In a productive collaborative followup study between Drs. Tigelaar, Girardi and Hayday with Richard Lifton (Genetics) recently published in Nature Genetics, it was shown that this defect is caused by mutation in Skint1, a newly identified gene expressed in thymus and skin that encodes a protein with immunoglobulin-like and transmembrane domains. Skint1 is the prototypic member of a rapidly evolving family of at least 11 genes in mouse, with greatest similarity to the butyrophilin genes. These findings define a new family of proteins mediating key epithelial-immune interactions.
- Characterizing the genes expressed by "resting" and "activated" DETC by serial analysis of gene expression (SAGE) and comparing such expression patterns with other IEL and recirculating T cell subsets.
- Investigating selected candidate DETC anti-inflammatory cytokines/effector molecules by reconstituting TCR delta-/- recipients with DETC precursors rendered deficient (via gene "knockout" or siRNA "knock-down") in candidate anti-inflammatory molecules.
- Utilizing genome-wide microsatellite mapping, to identify the genetic interval(s), and ultimately the specific genes, controlling susceptibility/resistance to the spontaneous dermatitis that develops in some, but not other TCR delta -/- mice.
- Utilizing cellular, molecular and genetic studies to identify the physiologic ligands for TCR gamma/delta+ T cells that trigger both their positive selection in the thymus and their activation in peripheral epithelial tissues such as the skin and GI tract.
Coauthors
Research Interests
Dermatitis, Atopic; Dermatitis, Contact; Dermatology; Immune System; Skin; Lymphoma, T-Cell, Cutaneous
Selected Publications
- Response: validity of evidence demonstrating efficacy of extracorporeal photochemotherapyBerger C, Lewis J, Girardi M, Tigelaar R, Edelson R. Response: validity of evidence demonstrating efficacy of extracorporeal photochemotherapy. Blood 2011, 117: 367-367. PMCID: PMC3037285, DOI: 10.1182/blood-2010-11-317107.
- Response: validity of evidence demonstrating efficacy of extracorporeal photochemotherapyBerger C, Lewis J, Girardi M, Tigelaar R, Edelson R. Response: validity of evidence demonstrating efficacy of extracorporeal photochemotherapy. Blood 2011, 117: 367-367. PMID: 21212292, PMCID: PMC3037285, DOI: 10.1182/blood-2010-11-317107.
- Skin immune surveillance by T cells—A new order?Strid J, Tigelaar RE, Hayday AC. Skin immune surveillance by T cells—A new order? Seminars In Immunology 2009, 21: 110-120. PMID: 19369094, DOI: 10.1016/j.smim.2009.03.002.
- Skint1, the prototype of a newly identified immunoglobulin superfamily gene cluster, positively selects epidermal γδ T cellsBoyden LM, Lewis JM, Barbee SD, Bas A, Girardi M, Hayday AC, Tigelaar RE, Lifton RP. Skint1, the prototype of a newly identified immunoglobulin superfamily gene cluster, positively selects epidermal γδ T cells. Nature Genetics 2008, 40: 656-662. PMID: 18408721, PMCID: PMC4167720, DOI: 10.1038/ng.108.
- Development of Immunogenic Tumor-Loaded Dendritic Cells Through Physical Perturbation and Apoptotic Cell LoadingShen X, Berger CL, Tigelaar R, Edelson RL. Development of Immunogenic Tumor-Loaded Dendritic Cells Through Physical Perturbation and Apoptotic Cell Loading. Immunological Investigations 2008, 37: 798-821. PMID: 18991097, DOI: 10.1080/08820130802403358.
- Characterizing tumor-promoting T cells in chemically induced cutaneous carcinogenesisRoberts SJ, Ng BY, Filler RB, Lewis J, Glusac EJ, Hayday AC, Tigelaar RE, Girardi M. Characterizing tumor-promoting T cells in chemically induced cutaneous carcinogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 6770-6775. PMID: 17412837, PMCID: PMC1871860, DOI: 10.1073/pnas.0604982104.
- Sustained localized expression of ligand for the activating NKG2D receptor impairs natural cytotoxicity in vivo and reduces tumor immunosurveillanceOppenheim DE, Roberts SJ, Clarke SL, Filler R, Lewis JM, Tigelaar RE, Girardi M, Hayday AC. Sustained localized expression of ligand for the activating NKG2D receptor impairs natural cytotoxicity in vivo and reduces tumor immunosurveillance. Nature Immunology 2005, 6: 928-937. PMID: 16116470, DOI: 10.1038/ni1239.
- The Integration of Conventional and Unconventional T Cells that Characterizes Cell‐Mediated ResponsesPennington D, Vermijlen D, Wise E, Clarke S, Tigelaar R, Hayday A. The Integration of Conventional and Unconventional T Cells that Characterizes Cell‐Mediated Responses. 2005, 87: 27-59. PMID: 16102571, DOI: 10.1016/s0065-2776(05)87002-6.
- Subset‐specific, uniform activation among Vγ6/Vδ1+ γδ T cells elicited by inflammationRoark C, Aydintug M, Lewis J, Yin X, Lahn M, Hahn Y, Born W, Tigelaar R, O’Brien R. Subset‐specific, uniform activation among Vγ6/Vδ1+ γδ T cells elicited by inflammation. Journal Of Leukocyte Biology 2003, 75: 68-75. PMID: 14525969, DOI: 10.1189/jlb.0703326.
- The inter-relatedness and interdependence of mouse T cell receptor γδ+ and αβ+ cellsPennington D, Silva-Santos B, Shires J, Theodoridis E, Pollitt C, Wise E, Tigelaar R, Owen M, Hayday A. The inter-relatedness and interdependence of mouse T cell receptor γδ+ and αβ+ cells. Nature Immunology 2003, 4: 991-998. PMID: 14502287, DOI: 10.1038/ni979.
- Regulation of Cutaneous Malignancy by γδ T CellsGirardi M, Oppenheim D, Steele C, Lewis J, Glusac E, Filler R, Hobby P, Sutton B, Tigelaar R, Hayday A. Regulation of Cutaneous Malignancy by γδ T Cells. Science 2001, 294: 605-609. PMID: 11567106, DOI: 10.1126/science.1063916.
- Induction of human tumor‐loaded dendritic cellsBerger C, Xu A, Hanlon D, Lee C, Schechner J, Glusac E, Christensen I, Snyder E, Holloway V, Tigelaar R, Edelson R. Induction of human tumor‐loaded dendritic cells. International Journal Of Cancer 2001, 91: 438-447. PMID: 11251964, DOI: 10.1002/1097-0215(200002)9999:9999<::aid-ijc1073>3.0.co;2-r.
- Development of Dendritic Epidermal T Cells with a Skewed Diversity of γδTCRs in Vδ1-Deficient MiceHara H, Kishihara K, Matsuzaki G, Takimoto H, Tsukiyama T, Tigelaar R, Nomoto K. Development of Dendritic Epidermal T Cells with a Skewed Diversity of γδTCRs in Vδ1-Deficient Mice. The Journal Of Immunology 2000, 165: 3695-3705. PMID: 11034374, DOI: 10.4049/jimmunol.165.7.3695.
- Granulocyte-Macrophage Colony-Stimulating Factor Priming plus Papillomavirus E6 DNA Vaccination: Effects on Papilloma Formation and Regression in the Cottontail Rabbit Papillomavirus-Rabbit ModelLeachman S, Tigelaar R, Shlyankevich M, Slade M, Irwin M, Chang E, Wu T, Xiao W, Pazhani S, Zelterman D, Brandsma J. Granulocyte-Macrophage Colony-Stimulating Factor Priming plus Papillomavirus E6 DNA Vaccination: Effects on Papilloma Formation and Regression in the Cottontail Rabbit Papillomavirus-Rabbit Model. Journal Of Virology 2000, 74: 8700-8708. PMID: 10954571, PMCID: PMC116381, DOI: 10.1128/jvi.74.18.8700-8708.2000.
- Enterocyte Expression of Interleukin 7 Induces Development of γδ T Cells and Peyer's PatchesLaky K, Lefrançois L, Lingenheld E, Ishikawa H, Lewis J, Olson S, Suzuki K, Tigelaar R, Puddington L. Enterocyte Expression of Interleukin 7 Induces Development of γδ T Cells and Peyer's Patches. Journal Of Experimental Medicine 2000, 191: 1569-1580. PMID: 10790431, PMCID: PMC2213426, DOI: 10.1084/jem.191.9.1569.
- Th2 responses induced by epicutaneous or inhalational protein exposure are differentially dependent on IL-4Herrick C, MacLeod H, Glusac E, Tigelaar R, Bottomly K. Th2 responses induced by epicutaneous or inhalational protein exposure are differentially dependent on IL-4. Journal Of Clinical Investigation 2000, 105: 765-775. PMID: 10727445, PMCID: PMC377464, DOI: 10.1172/jci8624.
- Stevens-Johnson syndrome limited to multiple sites of radiation therapy in a patient receiving phenobarbitalDuncan K, Tigelaar R, Bolognia J. Stevens-Johnson syndrome limited to multiple sites of radiation therapy in a patient receiving phenobarbital. Journal Of The American Academy Of Dermatology 1999, 40: 493-496. PMID: 10071329, DOI: 10.1016/s0190-9622(99)70508-6.
- Intracutaneous vaccination of rabbits with the E6 gene of cottontail rabbit papillomavirus provides partial protection against virus challengeSundaram P, Tigelaar R, Xiao W, Brandsma J. Intracutaneous vaccination of rabbits with the E6 gene of cottontail rabbit papillomavirus provides partial protection against virus challenge. Vaccine 1998, 16: 613-623. PMID: 9569473, DOI: 10.1016/s0264-410x(97)84510-0.
- Conservation of T Cell Receptor Conformation in Epidermal γδ Cells with Disrupted Primary Vγ Gene UsageMallick-Wood C, Lewis J, Richie L, Owen M, Tigelaar R, Hayday A. Conservation of T Cell Receptor Conformation in Epidermal γδ Cells with Disrupted Primary Vγ Gene Usage. Science 1998, 279: 1729-1733. PMID: 9497293, DOI: 10.1126/science.279.5357.1729.
- A polymerase chain reaction-based approach in search of potential T cell epitopes in pemphigus vulgarisSinha A, Shlyankevich M, Lin M, Diaz L, Tigelaar R, Ianeway C. A polymerase chain reaction-based approach in search of potential T cell epitopes in pemphigus vulgaris. Journal Of Dermatological Science 1998, 16: s42. DOI: 10.1016/s0923-1811(98)83250-7.
- Regulation of epicutaneously induced TH2 responses to soluble protein by interleukin-4Herrick C, Tigelaar R, Bottomly K. Regulation of epicutaneously induced TH2 responses to soluble protein by interleukin-4. Journal Of Dermatological Science 1998, 16: s4. DOI: 10.1016/s0923-1811(98)83022-3.
- Intracutaneous vaccination of rabbits with the cottontail rabbit papillomavirus (CRPV) L1 gene protects against virus challengeSundaram P, Tigelaar R, Brandsma J. Intracutaneous vaccination of rabbits with the cottontail rabbit papillomavirus (CRPV) L1 gene protects against virus challenge. Vaccine 1997, 15: 664-671. PMID: 9178468, DOI: 10.1016/s0264-410x(96)00237-x.
- Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase.Mallick-Wood C, Pao W, Cheng A, Lewis J, Kulkarni S, Bolen J, Rowley B, Tigelaar R, Pawson T, Hayday A. Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 9704-9709. PMID: 8790395, PMCID: PMC38493, DOI: 10.1073/pnas.93.18.9704.
- Thy‐1 + dendritic cells express truncated form of POMC mRNAFarooqui J, Medrano E, Boissy R, Tigelaar R, Nordlund J. Thy‐1 + dendritic cells express truncated form of POMC mRNA. Experimental Dermatology 1995, 4: 297-301. PMID: 8589920, DOI: 10.1111/j.1600-0625.1995.tb00208.x.
- Malignant and Normal T Cells Show Random Use of T-Cell Receptor ∝ Chain Variable Regions in Patients with Cutaneous T-Cell LymphomaLongley J, Tyrrell L, Lu S, Farrell J, Ding T, Yan S, Sallee D, Heald P, Berger C, Tigelaar R, Edelson R. Malignant and Normal T Cells Show Random Use of T-Cell Receptor ∝ Chain Variable Regions in Patients with Cutaneous T-Cell Lymphoma. Journal Of Investigative Dermatology 1995, 105: 62-64. PMID: 7615977, DOI: 10.1111/1523-1747.ep12312571.
- Murine Epidermal Vγ5/Vδ1-T-Cell Receptor+ T Cells Respond to B-Cell Lines and LipopolysaccharidesReardon C, Heyborne K, Tsuji M, Zavala F, Tigelaar R, O'Brien R, Born W. Murine Epidermal Vγ5/Vδ1-T-Cell Receptor+ T Cells Respond to B-Cell Lines and Lipopolysaccharides. Journal Of Investigative Dermatology 1995, 105: s58-s61. DOI: 10.1038/jid.1995.12.
- Immunobiology of Mouse Dendritic Epidermal T Cells: A Decade Later, Some Answers, But Still More QuestionsTigelaar R, Lewis J. Immunobiology of Mouse Dendritic Epidermal T Cells: A Decade Later, Some Answers, But Still More Questions. Journal Of Investigative Dermatology 1995, 105: s43-s49. PMID: 7615996, DOI: 10.1038/jid.1995.9.
- Murine epidermal V gamma 5/V delta 1-T-cell receptor+ T cells respond to B-cell lines and lipopolysaccharides.Reardon C, Heyborne K, Tsuji M, Zavala F, Tigelaar R, O'Brien R, Born W. Murine epidermal V gamma 5/V delta 1-T-cell receptor+ T cells respond to B-cell lines and lipopolysaccharides. Journal Of Investigative Dermatology 1995, 105: 58s-61s. PMID: 7615998, DOI: 10.1111/1523-1747.ep12316074.
- Immunobiology of Mouse Dendritic Epidermal T Cells: A Decade Later, Some Answers, But Still More Questions.Tigelaar R, Lewis J. Immunobiology of Mouse Dendritic Epidermal T Cells: A Decade Later, Some Answers, But Still More Questions. Journal Of Investigative Dermatology 1995, 105: 43s-49s. DOI: 10.1111/1523-1747.ep12315280.
- Regulation of Transgenic Class II Major Histocompatibility Genes in Murine Langerhans CellsLongley J, Ding T, Levin D, Lewis J, Edelson R, Tigelaar R, Flavell R. Regulation of Transgenic Class II Major Histocompatibility Genes in Murine Langerhans Cells. Journal Of Investigative Dermatology 1995, 104: 329-334. PMID: 7860996, DOI: 10.1111/1523-1747.ep12665365.
- Specific Suppression of Lupus-Like Graft-Versus-Host Disease Using Extracorporeal Photochemical Attenuation of Effector LymphocytesGirardi M, Herreid P, Tigelaar R. Specific Suppression of Lupus-Like Graft-Versus-Host Disease Using Extracorporeal Photochemical Attenuation of Effector Lymphocytes. Journal Of Investigative Dermatology 1995, 104: 177-182. PMID: 7829872, DOI: 10.1111/1523-1747.ep12612741.
- Targetoid hemangioma associated with pregnancy and the menstrual cycleMorganroth G, Tigelaar R, Longley B, Luck L, Leffell D. Targetoid hemangioma associated with pregnancy and the menstrual cycle. Journal Of The American Academy Of Dermatology 1995, 32: 282-284. PMID: 7829718, DOI: 10.1016/0190-9622(95)90149-3.
- Steven R. Kohn (1943–1994)Edelson R, Tigelaar R. Steven R. Kohn (1943–1994). Journal Of The American Academy Of Dermatology 1994, 31: 687-688. DOI: 10.1016/s0190-9622(08)81745-8.
- Superantigenic Staphylococcal Exotoxins Induce T-Cell Proliferation in the Presence of Langerhans Cells or Class II–Bearing Keratinocytes and Stimulate Keratinocytes to Produce T-Cell–Activating CytokinesTokura Y, Yagi J, O'Malley J, Lewis J, Takigawa M, Edelson R, Tigelaar R. Superantigenic Staphylococcal Exotoxins Induce T-Cell Proliferation in the Presence of Langerhans Cells or Class II–Bearing Keratinocytes and Stimulate Keratinocytes to Produce T-Cell–Activating Cytokines. Journal Of Investigative Dermatology 1994, 102: 31-38. PMID: 8288908, DOI: 10.1111/1523-1747.ep12371727.
- Factors Involved in the Localization and Activation of Murine γδ Positive Dendritic Epidermal T CellsTigelaar R, Lewis J. Factors Involved in the Localization and Activation of Murine γδ Positive Dendritic Epidermal T Cells. 1994, 39-55. DOI: 10.1007/978-1-4615-1861-7_4.
- THE TREATMENT OF MASTOCYTOMA CELLS WITH 8‐METHOXYPSORALEN AND LONG‐WAVELENGTH ULTRAVIOLET RADIATION ENHANCES CELLULAR IMMUNOGENICITY: PRELIMINARY RESULTSGasparro F, Malane M, Maxwell V, Tigelaar R. THE TREATMENT OF MASTOCYTOMA CELLS WITH 8‐METHOXYPSORALEN AND LONG‐WAVELENGTH ULTRAVIOLET RADIATION ENHANCES CELLULAR IMMUNOGENICITY: PRELIMINARY RESULTS. Photochemistry And Photobiology 1993, 58: 682-688. PMID: 8284324, DOI: 10.1111/j.1751-1097.1993.tb04952.x.
- Skin-Selective Lymphocyte Homing Mechanisms in the Pathogenesis of Leukemic Cutaneous T-Cell LymphomaHeald P, Yan S, Edelson R, Tigelaar R, Picker L. Skin-Selective Lymphocyte Homing Mechanisms in the Pathogenesis of Leukemic Cutaneous T-Cell Lymphoma. Journal Of Investigative Dermatology 1993, 101: 222-226. PMID: 7688404, DOI: 10.1111/1523-1747.ep12364814.
- Idiopathic CD4+ Lymphocyte Deficiency: Report of an Unusual Case Associated With Atopic Dermatitis and Allergic Contact Dermatitis and Review of the LiteratureGoodrich A, Tigelaar R, Watsky K, Heald P. Idiopathic CD4+ Lymphocyte Deficiency: Report of an Unusual Case Associated With Atopic Dermatitis and Allergic Contact Dermatitis and Review of the Literature. JAMA Dermatology 1993, 129: 876-878. DOI: 10.1001/archderm.1993.01680280064012.
- Idiopathic CD4+ lymphocyte deficiency. Report of an unusual case associated with atopic dermatitis and allergic contact dermatitis and review of the literature.Goodrich A, Tigelaar R, Watsky K, Heald P. Idiopathic CD4+ lymphocyte deficiency. Report of an unusual case associated with atopic dermatitis and allergic contact dermatitis and review of the literature. JAMA Dermatology 1993, 129: 876-8. PMID: 8100699, DOI: 10.1001/archderm.129.7.876.
- Expression of mouse Tla region class I genes in tissues enriched for γδ cellsEghtesady P, Brorson K, Cheroutre H, Tigelaar R, Hood L, Kronenberg M. Expression of mouse Tla region class I genes in tissues enriched for γδ cells. Immunogenetics 1993, 38: 80-80. DOI: 10.1007/bf00216400.
- Expression of mouse Tla region class I genes in tissue enriched for γδ cellsEghtesady P, Brorson K, Cheroutre H, Tigelaar R, Hood L, Kronenberg M. Expression of mouse Tla region class I genes in tissue enriched for γδ cells. Immunogenetics 1992, 36: 377-388. PMID: 1388139, DOI: 10.1007/bf00218045.
- SÉZARY CELL PREP TEST RE-EVALUATEDLawrence N, McBurney E, Tigelaar R, Cockerell C. SÉZARY CELL PREP TEST RE-EVALUATED. Southern Medical Journal 1992, 85: 3s-22. DOI: 10.1097/00007611-199209001-00058.
- Peptidergic Neurons and Vasoactive Intestinal Peptide Modulate Experimental Delayed‐Type Hypersensitivity ReactionsaGIROLOMONI G, TIGELAAR R. Peptidergic Neurons and Vasoactive Intestinal Peptide Modulate Experimental Delayed‐Type Hypersensitivity Reactionsa. Annals Of The New York Academy Of Sciences 1992, 650: 9-12. PMID: 1605501, DOI: 10.1111/j.1749-6632.1992.tb49087.x.
- T Lymphocytes Bearing Gamma-Delta Antigen Receptors in SkinModlin R, Lewis J, Uyemura K, Tigelaar R. T Lymphocytes Bearing Gamma-Delta Antigen Receptors in Skin. 1992, 53: 61-74. DOI: 10.1159/000421137.
- T Lymphocytes Bearing Gamma-Delta Antigen Receptors in SkinModlin R, Lewis J, Uyemura K, Tigelaar R. T Lymphocytes Bearing Gamma-Delta Antigen Receptors in Skin. 1992, 53: 61-74. PMID: 1534236, DOI: 10.1159/000319103.
- Murine epidermal γ/δ T cells express Fcγ receptor II encoded by the FcγRα α geneKuziel W, Lewis J, Nixon‐Fulton J, Tigelaar R, Tucker P. Murine epidermal γ/δ T cells express Fcγ receptor II encoded by the FcγRα α gene. European Journal Of Immunology 1991, 21: 1563-1566. PMID: 1828429, DOI: 10.1002/eji.1830210635.
- Adhesion Molecules CD11a, CD18, and ICAM-1 on Human Epidermal Langerhans Cells Serve a Functional Role in the Activation of Alloreactive T CellsSimon J, Cruz P, Tigelaar R, Sontheimer R, Bergstresser P. Adhesion Molecules CD11a, CD18, and ICAM-1 on Human Epidermal Langerhans Cells Serve a Functional Role in the Activation of Alloreactive T Cells. Journal Of Investigative Dermatology 1991, 96: 148-151. PMID: 1670950, DOI: 10.1111/1523-1747.ep12515946.
- Capsaicin-sensitive primary sensory neurons are potent modulators of murine delayed-type hypersensitivity reactions.Girolomoni G, Tigelaar R. Capsaicin-sensitive primary sensory neurons are potent modulators of murine delayed-type hypersensitivity reactions. The Journal Of Immunology 1990, 145: 1105-12. PMID: 2380551, DOI: 10.4049/jimmunol.145.4.1105.
- TCR γ/δ+ Dendritic Epidermal T Cells as Constituents of Skin-Associated Lymphoid TissueTigelaar R, Lewis J, Bergstresser P. TCR γ/δ+ Dendritic Epidermal T Cells as Constituents of Skin-Associated Lymphoid Tissue. Journal Of Investigative Dermatology 1990, 94: s58-s63. PMID: 1972173, DOI: 10.1111/1523-1747.ep12875138.
- Roles of CD4+ and CD8+ T cells in murine contact sensitivity revealed by in vivo monoclonal antibody depletion.Gocinski B, Tigelaar R. Roles of CD4+ and CD8+ T cells in murine contact sensitivity revealed by in vivo monoclonal antibody depletion. The Journal Of Immunology 1990, 144: 4121-8. PMID: 1971294, DOI: 10.4049/jimmunol.144.11.4121.
- Langerhans cells that migrate to skin after intravenous infusion regulate the induction of contact hypersensitivity.Cruz P, Tigelaar R, Bergstresser P. Langerhans cells that migrate to skin after intravenous infusion regulate the induction of contact hypersensitivity. The Journal Of Immunology 1990, 144: 2486-92. PMID: 1969448, DOI: 10.4049/jimmunol.144.7.2486.
- UVB Radiation and DNFB Skin Painting Induce Suppressor Cells Universally in MiceGlass M, Bergstresser P, Tigelaar R, Streilein J. UVB Radiation and DNFB Skin Painting Induce Suppressor Cells Universally in Mice. Journal Of Investigative Dermatology 1990, 94: 273-278. PMID: 1689757, DOI: 10.1111/1523-1747.ep12874117.
- Limited diversity of T-cell receptor gamma-chain expression of murine Thy-1+ dendritic epidermal cells revealed by V gamma 3-specific monoclonal antibody.Havran W, Grell S, Duwe G, Kimura J, Wilson A, Kruisbeek A, O'Brien R, Born W, Tigelaar R, Allison J. Limited diversity of T-cell receptor gamma-chain expression of murine Thy-1+ dendritic epidermal cells revealed by V gamma 3-specific monoclonal antibody. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 4185-4189. PMID: 2726770, PMCID: PMC287415, DOI: 10.1073/pnas.86.11.4185.
- Phenotypic and functional analysis of gamma delta T cell receptor-positive murine dendritic epidermal clones.Havran W, Poenie M, Tigelaar R, Tsien R, Allison J. Phenotypic and functional analysis of gamma delta T cell receptor-positive murine dendritic epidermal clones. The Journal Of Immunology 1989, 142: 1422-8. PMID: 2783942, DOI: 10.4049/jimmunol.142.5.1422.
- Disparate Effects of In Vitro Low-Dose UVB Irradiation on Intravenous Immunization with Purified Epidermal Cell Subpopulations for the Induction of Contact HypersensitivityCruz P, Nixon-Fulton J, Tigelaar R, Bergstresser P. Disparate Effects of In Vitro Low-Dose UVB Irradiation on Intravenous Immunization with Purified Epidermal Cell Subpopulations for the Induction of Contact Hypersensitivity. Journal Of Investigative Dermatology 1989, 92: 160-165. PMID: 2918229, DOI: 10.1111/1523-1747.ep12276682.
- Limited diversity of γδ antigen receptor genes of thy-1+ dendritic epidermal cellsAsarnow D, Kuziel W, Bonyhad M, Tigelaar R, Tucker P, Allison J. Limited diversity of γδ antigen receptor genes of thy-1+ dendritic epidermal cells. Cell 1988, 55: 837-847. PMID: 2847872, DOI: 10.1016/0092-8674(88)90139-0.
- γδ Antigen receptors of Thy-1 + dendritic epidermal cells: Implications for thymic differentiationAllison J, Hayran W, Asarnow D, Tigelaar R, Tucker P, Bonyhadi M. γδ Antigen receptors of Thy-1 + dendritic epidermal cells: Implications for thymic differentiation. Immunologic Research 1988, 7: 292. PMID: 2906352, DOI: 10.1007/bf02935534.
- Effect of Keratinocyte Cytokines on Thy‐1+ Dendritic Epidermal CellsTIGELAAR R, NIXON‐FULTON J, TAKASHIMA A, KUZIEL W, TAKIJIRI C, LEWIS J, TUCKER P, BERGSTRESSER P. Effect of Keratinocyte Cytokines on Thy‐1+ Dendritic Epidermal Cells. Annals Of The New York Academy Of Sciences 1988, 548: 271-282. PMID: 2470301, DOI: 10.1111/j.1749-6632.1988.tb18815.x.
- Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice. A study of morphology, function, and T cell receptor expression.Nixon-Fulton J, Kuziel W, Santerse B, Bergstresser P, Tucker P, Tigelaar R. Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice. A study of morphology, function, and T cell receptor expression. The Journal Of Immunology 1988, 141: 1897-903. PMID: 2902136, DOI: 10.4049/jimmunol.141.6.1897.
- Phenotypic Heterogeneity and Cytotoxic Activity of Con A and IL-2-Stimulated Cultures of Mouse Thy-1+ Epidermal CellsNixon-Fulton J, Hackett J, Bergstresser P, Kumar V, Tigelaar R. Phenotypic Heterogeneity and Cytotoxic Activity of Con A and IL-2-Stimulated Cultures of Mouse Thy-1+ Epidermal Cells. Journal Of Investigative Dermatology 1988, 91: 62-68. PMID: 2898506, DOI: 10.1111/1523-1747.ep12463290.
- Local effects of UV radiation on immunization with contact sensitizers. I. Down-regulation of contact hypersensitivity by application of TNCB to UV-irradiated skin.Cruz P, Nixon-Fulton J, Tigelaar R, Bergstresser P. Local effects of UV radiation on immunization with contact sensitizers. I. Down-regulation of contact hypersensitivity by application of TNCB to UV-irradiated skin. Photodermatology Photoimmunology & Photomedicine 1988, 5: 126-32. PMID: 3174491.
- Thy-1+ Dendritic Epidermal Cells in Mice: Precursor Frequency Analysis and Cloning of Concanavalin A-Reactive CellsTakashima A, Nixon-Fulton J, Bergstresser P, Tigelaar R. Thy-1+ Dendritic Epidermal Cells in Mice: Precursor Frequency Analysis and Cloning of Concanavalin A-Reactive Cells. Journal Of Investigative Dermatology 1988, 90: 671-678. PMID: 2896217, DOI: 10.1111/1523-1747.ep12560835.
- Delta is the Cx-gene product in the γ/δ antigen receptor of dendritic epidermal cellsBonyhadi M, Weiss A, Tucker P, Tigelaar R, Allison J. Delta is the Cx-gene product in the γ/δ antigen receptor of dendritic epidermal cells. Nature 1987, 330: 574-576. PMID: 3500417, DOI: 10.1038/330574a0.
- Hyperkeratotic Papules on the Hands and FeetBlum S, Cruz P, Siegel D, Tigelaar R. Hyperkeratotic Papules on the Hands and Feet. JAMA Dermatology 1987, 123: 1225-1226. PMID: 3632007, DOI: 10.1001/archderm.1987.01660330136027.
- Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cellsKuziel W, Takashima A, Bonyhadi M, Bergstresser P, Allison J, Tigelaar R, Tucker P. Regulation of T-cell receptor γ-chain RNA expression in murine Thy-1+ dendritic epidermal cells. Nature 1987, 328: 263-266. PMID: 2885757, DOI: 10.1038/328263a0.
- Lack of dendritic Thy-1+ epidermal cells in mice with severe combined immunodeficiency disease.Nixon-Fulton J, Witte P, Tigelaar R, Bergstresser P, Kumar V. Lack of dendritic Thy-1+ epidermal cells in mice with severe combined immunodeficiency disease. The Journal Of Immunology 1987, 138: 2902-5. PMID: 2883236, DOI: 10.4049/jimmunol.138.9.2902.
- Induction and regulation of contact hypersensitivity by resident, bone marrow-derived, dendritic epidermal cells: Langerhans cells and Thy-1+ epidermal cells.Sullivan S, Bergstresser P, Tigelaar R, Streilein J. Induction and regulation of contact hypersensitivity by resident, bone marrow-derived, dendritic epidermal cells: Langerhans cells and Thy-1+ epidermal cells. The Journal Of Immunology 1986, 137: 2460-7. PMID: 2876041, DOI: 10.4049/jimmunol.137.8.2460.
- Ataxia‐telangiectasia Associated with SarcoidosisFleck R, Myers L, Wasserman R, Tigelaar R, Freeman R. Ataxia‐telangiectasia Associated with Sarcoidosis. Pediatric Dermatology 1986, 3: 339-343. PMID: 3774655, DOI: 10.1111/j.1525-1470.1986.tb00537.x.
- Thy-1+ epidermal cells proliferate in response to concanavalin A and interleukin 2.Nixon-Fulton J, Bergstresser P, Tigelaar R. Thy-1+ epidermal cells proliferate in response to concanavalin A and interleukin 2. The Journal Of Immunology 1986, 136: 2776-86. PMID: 2870120, DOI: 10.4049/jimmunol.136.8.2776.
- Lymphocyte Traffic and the SkinTigelaar R. Lymphocyte Traffic and the Skin. Dermatologic Clinics 1985, 3: 569-585. PMID: 3916171, DOI: 10.1016/s0733-8635(18)30857-x.
- Origin and Function of Thy-1+ Dendritic Epidermal Cells in MiceBergstresser P, Sullivan S, Streilein J, Tigelaar R. Origin and Function of Thy-1+ Dendritic Epidermal Cells in Mice. Journal Of Investigative Dermatology 1985, 85: s85-s90. PMID: 2409184, DOI: 10.1111/1523-1747.ep12275516.
- FACS Purification of Bone Marrow-Derived Epidermal Populations in Mice: Langerhans Cells and Thy-1+ Dendritic CellsSullivan S, Bergstresser P, Tigelaar R, Streilein J. FACS Purification of Bone Marrow-Derived Epidermal Populations in Mice: Langerhans Cells and Thy-1+ Dendritic Cells. Journal Of Investigative Dermatology 1985, 84: 491-495. PMID: 3998496, DOI: 10.1111/1523-1747.ep12273454.
- Conjugated avidin binds to mast cell granules.Tharp M, Seelig L, Tigelaar R, Bergstresser P. Conjugated avidin binds to mast cell granules. Journal Of Histochemistry & Cytochemistry 1985, 33: 27-32. PMID: 2578142, DOI: 10.1177/33.1.2578142.
- The Intracutaneous Growth of Murine Lymphomas: Epidermal Invasion Is Characteristic of Multiple Tumor PhenotypesPiepkorn M, Tigelaar R. The Intracutaneous Growth of Murine Lymphomas: Epidermal Invasion Is Characteristic of Multiple Tumor Phenotypes. Journal Of Investigative Dermatology 1984, 83: 281-285. PMID: 6237158, DOI: 10.1111/1523-1747.ep12340368.
- Conjugated Avidin Identifies Cutaneous Rodent and Human Mast CellsBergstresser P, Tigelaar R, Tharp M. Conjugated Avidin Identifies Cutaneous Rodent and Human Mast Cells. Journal Of Investigative Dermatology 1984, 83: 214-218. PMID: 6470526, DOI: 10.1111/1523-1747.ep12263584.
- Thy-1 Antigen-Bearing Dendritic Cells in Murine Epidermis Are Derived from Bone Marrow PrecursorsBergstresser P, Tigelaar R, Streilein J. Thy-1 Antigen-Bearing Dendritic Cells in Murine Epidermis Are Derived from Bone Marrow Precursors. Journal Of Investigative Dermatology 1984, 83: 83-87. PMID: 6147381, DOI: 10.1111/1523-1747.ep12262587.
- Analysis of the mechanism of unresponsiveness produced by haptens painted on skin exposed to low dose ultraviolet radiation.Elmets C, Bergstresser P, Tigelaar R, Wood P, Streilein J. Analysis of the mechanism of unresponsiveness produced by haptens painted on skin exposed to low dose ultraviolet radiation. Journal Of Experimental Medicine 1983, 158: 781-794. PMID: 6193234, PMCID: PMC2187099, DOI: 10.1084/jem.158.3.781.
- Thy-1 Antigen-Bearing Dendritic Cells Populate Murine EpidermisBergstresser P, Tigelaar R, Dees J, Streilein J. Thy-1 Antigen-Bearing Dendritic Cells Populate Murine Epidermis. Journal Of Investigative Dermatology 1983, 81: 286-288. PMID: 6136547, DOI: 10.1111/1523-1747.ep12518332.
- Contact hypersensitivity reactions to dinitrofluorobenzene mediated by monoclonal IgE anti-DNP antibodies.Ray M, Tharp M, Sullivan T, Tigelaar R. Contact hypersensitivity reactions to dinitrofluorobenzene mediated by monoclonal IgE anti-DNP antibodies. The Journal Of Immunology 1983, 131: 1096-102. PMID: 6193174, DOI: 10.4049/jimmunol.131.3.1096.
- SALT: Skin-Associated Lymphoid TissuesStreilein J, Tigelaar R. SALT: Skin-Associated Lymphoid Tissues. 1983, 95-130. DOI: 10.1007/978-1-4613-3670-9_6.
- Oral ingestion of epicutaneously applied hapten in mice may unwittingly induce down-regulation of contact hypersensitivity.Tigelaar R, Bergstresser P, Lonsberry L, Elmets C, Wood P, Streilen J. Oral ingestion of epicutaneously applied hapten in mice may unwittingly induce down-regulation of contact hypersensitivity. The Journal Of Immunology 1982, 129: 1898-904. PMID: 6749982, DOI: 10.4049/jimmunol.129.5.1898.
- Specific loss of stimulator activity following mixed lymphocyte reactions is due to cytotoxic cellsJaneway C, Mosier D, Tigelaar R. Specific loss of stimulator activity following mixed lymphocyte reactions is due to cytotoxic cells. European Journal Of Immunology 1976, 6: 747-750. PMID: 138373, DOI: 10.1002/eji.1830061017.
- Graft-versus-host reactivity of mouse thymocytes: Effect of in vitro treatment with anti-TL serumTigelaar R, Gershon R, Asofsky R. Graft-versus-host reactivity of mouse thymocytes: Effect of in vitro treatment with anti-TL serum. Cellular Immunology 1975, 19: 58-64. PMID: 241492, DOI: 10.1016/0008-8749(75)90291-9.
- SYNERGY AMONG LYMPHOID CELLS MEDIATING THE GRAFT-VERSUS-HOST RESPONSETigelaar R, Asofsky R. SYNERGY AMONG LYMPHOID CELLS MEDIATING THE GRAFT-VERSUS-HOST RESPONSE. Journal Of Experimental Medicine 1973, 137: 239-253. PMID: 4405532, PMCID: PMC2139477, DOI: 10.1084/jem.137.2.239.
- Graft-vs-host reactivity of mouse thymocytes: effect of cortisone pretreatment of donors.Tigelaar R, Asofsky R. Graft-vs-host reactivity of mouse thymocytes: effect of cortisone pretreatment of donors. The Journal Of Immunology 1973, 110: 567-74. PMID: 4405321, DOI: 10.4049/jimmunol.110.2.567.
- KINETICS OF GRAFT-VERSUS-HOST SPLENOMEGALY IN NEONATAL F1 RECIPIENTS OF PARENTAL STRAIN MOUSE LYMPH NODE CELLS AND/OR THYMOCYTESTigelaar R, Asofsky R. KINETICS OF GRAFT-VERSUS-HOST SPLENOMEGALY IN NEONATAL F1 RECIPIENTS OF PARENTAL STRAIN MOUSE LYMPH NODE CELLS AND/OR THYMOCYTES. Transplantation 1972, 14: 805-807. PMID: 4404987, DOI: 10.1097/00007890-197212000-00025.
- SYNERGY AMONG LYMPHOID CELLS MEDIATING THE GRAFT-VERSUS-HOST RESPONSETigelaar R, Asofsky R. SYNERGY AMONG LYMPHOID CELLS MEDIATING THE GRAFT-VERSUS-HOST RESPONSE. Journal Of Experimental Medicine 1972, 135: 1059-1070. PMID: 4401814, PMCID: PMC2138974, DOI: 10.1084/jem.135.5.1059.
- Cell Interactions in the Graft-versus-Host ResponseASOFSKY R, CANTOR H, TIGELAAR R. Cell Interactions in the Graft-versus-Host Response. 1971, 369-381. DOI: 10.1016/b978-0-12-057550-3.50034-x.