2021
Validation of a Gas Chromatography-Mass Spectrometry Method for the Measurement of the Redox State Metabolic Ratios Lactate/Pyruvate and β-Hydroxybutyrate/Acetoacetate in Biological Samples
Wijngaard R, Perramón M, Parra-Robert M, Hidalgo S, Butrico G, Morales-Ruiz M, Zeng M, Casals E, Jiménez W, Fernández-Varo G, Shulman GI, Cline GW, Casals G. Validation of a Gas Chromatography-Mass Spectrometry Method for the Measurement of the Redox State Metabolic Ratios Lactate/Pyruvate and β-Hydroxybutyrate/Acetoacetate in Biological Samples. International Journal Of Molecular Sciences 2021, 22: 4752. PMID: 33946157, PMCID: PMC8125771, DOI: 10.3390/ijms22094752.Peer-Reviewed Original Research3-Hydroxybutyric AcidAcetoacetatesAnimalsFemaleGas Chromatography-Mass SpectrometryHep G2 CellsHumansLactatesLimit of DetectionLiverOxidation-ReductionPyruvatesRats, Wistar
2000
Contrasting Effects of IRS-1 Versus IRS-2 Gene Disruption on Carbohydrate and Lipid Metabolism in Vivo *
Previs S, Withers D, Ren J, White M, Shulman G. Contrasting Effects of IRS-1 Versus IRS-2 Gene Disruption on Carbohydrate and Lipid Metabolism in Vivo *. Journal Of Biological Chemistry 2000, 275: 38990-38994. PMID: 10995761, DOI: 10.1074/jbc.m006490200.Peer-Reviewed Original ResearchMeSH KeywordsAdipose TissueAnimalsCarbohydrate MetabolismFatty Acids, NonesterifiedFood DeprivationGas Chromatography-Mass SpectrometryGlucoseGlycerolInsulinInsulin Receptor Substrate ProteinsIntracellular Signaling Peptides and ProteinsLipid MetabolismLiverMaleMiceMusclesMutationPhenotypePhosphoproteinsRadioimmunoassayTime FactorsConceptsLipid metabolismInsulin resistanceIRS-2Glucose utilizationPlasma free fatty acid concentrationsWhole-body glucose utilizationGlycerol turnoverFree fatty acid concentrationsMarked insulin resistancePeripheral glucose metabolismPeripheral glucose utilizationHyperinsulinemic-euglycemic clampEndogenous glucose productionIRS-1Effect of insulinHepatic glycogen synthesisWT miceFatty acid concentrationsInsulin receptor substrateGlucose metabolismFasted miceAdipose tissueReduced suppressionGlucose productionMiceIntense exercise stimulates albumin synthesis in the upright posture
Nagashima K, Cline G, Mack G, Shulman G, Nadel E. Intense exercise stimulates albumin synthesis in the upright posture. Journal Of Applied Physiology 2000, 88: 41-46. PMID: 10642360, DOI: 10.1152/jappl.2000.88.1.41.Peer-Reviewed Original ResearchAdultDeuteriumExerciseFemaleGas Chromatography-Mass SpectrometryHumansKineticsMaleOxygen ConsumptionPhenylalaninePlasma VolumePostureSerum AlbuminSupine Position