Stacy Malaker
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About
Titles
Assistant Professor
Biography
Dr. Malaker is an Assistant Professor in the Department of Chemistry at Yale University. Her laboratory is focused on establishing methods and technology to study mucins, a class of densely O-glycosylated extracellular proteins, by MS. Additionally, the laboratory studies mucins in a biological context, since these proteins play integral, yet poorly understood, roles in numerous diseases. Prior to her appointment at Yale, she received her B.S. from the University of Michigan in Biochemistry and Anthropology-Zoology. Dr. Malaker then went on to receive her PhD in Chemistry from the University of Virginia in the laboratory of Professor Donald Hunt. She continued to investigate the role of aberrant glycosylation in cancer as an NIH postdoctoral fellow in Professor Carolyn Bertozzi’s laboratory at Stanford University before starting at Yale in 2021.
Appointments
Chemistry
Assistant ProfessorPrimary
Other Departments & Organizations
Research
Publications
2024
Analysis of Mucin‐Domain Glycoproteins Using Mass Spectrometry
Mahoney K, Malaker S. Analysis of Mucin‐Domain Glycoproteins Using Mass Spectrometry. Current Protocols 2024, 4: e1100. PMID: 38984456, PMCID: PMC11239139, DOI: 10.1002/cpz1.1100.Peer-Reviewed Original ResearchGlycoproteomics: Charting new territory in mass spectrometry and glycobiology
Malaker S. Glycoproteomics: Charting new territory in mass spectrometry and glycobiology. Journal Of Mass Spectrometry 2024, 59: e5034. PMID: 38726698, DOI: 10.1002/jms.5034.Peer-Reviewed Original ResearchStress-induced mucin 13 reductions drive intestinal microbiome shifts and despair behaviors
Rivet-Noor C, Merchak A, Render C, Gay N, Beiter R, Brown R, Keeler A, Moreau G, Li S, Olgun D, Steigmeyer A, Ofer R, Phan T, Vemuri K, Chen L, Mahoney K, Shin J, Malaker S, Deppmann C, Verzi M, Gaultier A. Stress-induced mucin 13 reductions drive intestinal microbiome shifts and despair behaviors. Brain Behavior And Immunity 2024, 119: 665-680. PMID: 38579936, PMCID: PMC11187485, DOI: 10.1016/j.bbi.2024.03.028.Peer-Reviewed Original ResearchMicrobiome shiftsDespair behaviorTranscription factor familyChronic stressRegulation of mucinsState of dysbiosisMicrobiome composition changesAssociated with disease pathologyDepressive-like symptomsModel of chronic stressLimited treatment optionsContext of stressHuman microbiomeMicrobiome compositionPsychological stress exposureMicrobial compositionFactor familyMicrobial dysbiosisMicrobial changesMicrobiome dysbiosisMicrobiomeTreatment optionsUpstream mediatorDepressive symptomsStress exposureMass Spectrometry-Compatible Elution Technique Enables an Improved Mucin-Selective Enrichment Strategy to Probe the Mucinome
Mahoney K, Chang V, Lucas T, Maruszko K, Malaker S. Mass Spectrometry-Compatible Elution Technique Enables an Improved Mucin-Selective Enrichment Strategy to Probe the Mucinome. Analytical Chemistry 2024, 96: 5242-5250. PMID: 38512228, DOI: 10.1021/acs.analchem.3c05762.Peer-Reviewed Original ResearchMucin-domain glycoproteinsO-glycopeptidesGlycopeptide signalsIn-gel digestionMass spectrometryElution conditionsSolid supportBinding moietyElution stepDownstream analysisO-glycosylationIn-gelBiological functionsElutionHuman serumEnrichment strategyCell linesMoietyEffective isolationSpectrometryCatalyticallyGlycoproteinSampling requirementsGlycopeptidesStcEGlycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SME and molecular dynamics simulations
Rosenfeld M, Kearns F, Chongsaritsinsuk J, Steigmeyer A, Mahoney K, Lucas T, Ince D, Battison A, Hollenhorst M, Shon D, Bertozzi C, Farracane M, Lemmon M, Malaker S, Amaro R. Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SME and molecular dynamics simulations. Biophysical Journal 2024, 123: 14a. DOI: 10.1016/j.bpj.2023.11.205.Peer-Reviewed Original Research
2023
Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE
Chongsaritsinsuk J, Steigmeyer A, Mahoney K, Rosenfeld M, Lucas T, Smith C, Li A, Ince D, Kearns F, Battison A, Hollenhorst M, Judy Shon D, Tiemeyer K, Attah V, Kwon C, Bertozzi C, Ferracane M, Lemmon M, Amaro R, Malaker S. Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE. Nature Communications 2023, 14: 6169. PMID: 37794035, PMCID: PMC10550946, DOI: 10.1038/s41467-023-41756-y.Peer-Reviewed Original ResearchConceptsFamily of proteinsMucin domainO-glycosylationBiological functionsKey regulatorComplex glycansMass spectrometric analysisFunctional relevanceTIM familyDetailed molecular structureCritical roleGlycosylationProteinSpectrometric analysisStructural featuresUnique abilityStructural dynamicsMolecular dynamics simulationsTim-3 functionFamilyPowerful workflowRegulatorImmune cellsCheckpointGlycansFalse-Positive Glycopeptide Identification via In-FAIMS Fragmentation
Rangel-Angarita V, Mahoney K, Kwon C, Sarker R, Lucas T, Malaker S. False-Positive Glycopeptide Identification via In-FAIMS Fragmentation. JACS Au 2023, 3: 2498-2509. PMID: 37772174, PMCID: PMC10523363, DOI: 10.1021/jacsau.3c00264.Peer-Reviewed Original ResearchDesign of a mucin-selective protease for targeted degradation of cancer-associated mucins
Pedram K, Shon D, Tender G, Mantuano N, Northey J, Metcalf K, Wisnovsky S, Riley N, Forcina G, Malaker S, Kuo A, George B, Miller C, Casey K, Vilches-Moure J, Ferracane M, Weaver V, Läubli H, Bertozzi C. Design of a mucin-selective protease for targeted degradation of cancer-associated mucins. Nature Biotechnology 2023, 42: 597-607. PMID: 37537499, PMCID: PMC11018308, DOI: 10.1038/s41587-023-01840-6.Peer-Reviewed Original ResearchCancer progressionSpecific protein glycoformsUndruggable proteinsTargeted degradationProtein degradationBreast cancer progressionCell surface bindingSubstrate selectivityCell-type selectivityCell deathGlycan motifsProtein glycoformsDiscrete peptidesCancer cellsProteinCulture modelProteaseTarget cellsTumor growthCancer-associated mucinsCellsMouse modelDegradersGrowthMotifMutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activity
Hollander M, Malaker S, Riley N, Perez I, Abney N, Gray M, Maxson J, Cochran J, Bertozzi C. Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activity. Journal Of Biological Chemistry 2023, 299: 104755. PMID: 37116708, PMCID: PMC10245049, DOI: 10.1016/j.jbc.2023.104755.Peer-Reviewed Original ResearchConceptsProtein domain mappingSingle amino acid mutationLigand-independent activityChronic neutrophilic leukemiaCell surface receptorsAmino acid mutationsColony-stimulating factor 3 receptorSerine residuesPresence of GalNAcHotspot residuesNeutrophilic leukemiaHuman diseasesAcid mutationsReceptor signalingAmino acidsCell growthSurface receptorsReceptor alpha chainMutationsAbundant typeDomain mappingDisease mechanismsUncontrolled activityAlpha chainWhite blood cellsVibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization
Huang X, Nero T, Weerasekera R, Matej K, Hinbest A, Jiang Z, Lee R, Wu L, Chak C, Nijjer J, Gibaldi I, Yang H, Gamble N, Ng W, Malaker S, Sumigray K, Olson R, Yan J. Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization. Nature Communications 2023, 14: 2104. PMID: 37055389, PMCID: PMC10102183, DOI: 10.1038/s41467-023-37660-0.Peer-Reviewed Original ResearchConceptsBiofilm matrix exopolysaccharideFacilitate host colonizationVibrio cholerae biofilmsΒ-propeller domainMatrix exopolysaccharideModular domainsHost colonizationRedundant rolesDistinct functionsAbiotic surfacesAdhesive proteinsHost surfaceHuman pathogensVibrio choleraeAdhesinsBacterial biofilmsHost tissuesColonization modelColonizationBAP1BiofilmsPathogensAntibiotic resistanceRBMCDomain
Academic Achievements and Community Involvement
Links & Media
News
- September 08, 2023
WHRY Launches New Pilot Project Studies
- May 25, 2023Source: NBC CT
Yale Researcher Studying How to Detect Ovarian Cancer Earlier
- May 16, 2023
Women’s Health Research at Yale Funds Studies to Improve Early Detection of Ovarian Cancer, Treatment of Prenatal Infections, and Aortic Aneurysm Outcomes in Women