Jing Yan, PhD
Assistant Professor, Molecular, Cellular and Developmental BiologyCards
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Featured Publications
Bacteria surfing the elastic wave
Nijjer J, Cohen T, Yan J. Bacteria surfing the elastic wave. Nature Physics 2022, 19: 6-7. DOI: 10.1038/s41567-022-01862-y.Peer-Reviewed Original ResearchSocial evolution of shared biofilm matrix components
Tai J, Mukherjee S, Nero T, Olson R, Tithof J, Nadell C, Yan J. Social evolution of shared biofilm matrix components. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2123469119. PMID: 35771939, PMCID: PMC9271185, DOI: 10.1073/pnas.2123469119.Peer-Reviewed Original ResearchConceptsBiofilm matrix componentsCooperative public goodsMatrix componentsBiofilm formationBiofilm matrix proteinsMatrix productionSocial evolution theoryBiofilm spatial structureCell surface adhesionEvolutionary timescalesCell clustersRelatedness coefficientsBacterial communitiesBiofilm habitatAdhesion proteinsEvolutionary advantageEvolutionary stabilityMatrix proteinsAssociated analysis toolsDiffusible compoundsHost environmentSocial evolutionUbiquitous modeOutstanding questionsProteinMechanical Resilience of Biofilms toward Environmental Perturbations Mediated by Extracellular Matrix
Zhang Q, Nguyen D, Tai J, Xu X, Nijjer J, Huang X, Li Y, Yan J. Mechanical Resilience of Biofilms toward Environmental Perturbations Mediated by Extracellular Matrix. Advanced Functional Materials 2022, 32 DOI: 10.1002/adfm.202110699.Peer-Reviewed Original ResearchMechanical resilienceNonlinear viscoelastic behaviorMaterials science pointLarge shear forcesBiofilm removal strategiesMechanical behaviorViscoelastic materialsViscoelastic behaviorBiofilm mechanicsSoft materialsStructure-property relationshipsRheological regimesRheological measurementsShear forceFunctional soft materialsLarge mechanical perturbationsPhysical insightPolymeric networkRemoval strategiesDynamics simulationsMolecular dynamics simulationsMaterialsDynamic environmentMatrixMechanical perturbationsMechanical forces drive a reorientation cascade leading to biofilm self-patterning
Nijjer J, Li C, Zhang Q, Lu H, Zhang S, Yan J. Mechanical forces drive a reorientation cascade leading to biofilm self-patterning. Nature Communications 2021, 12: 6632. PMID: 34789754, PMCID: PMC8599862, DOI: 10.1038/s41467-021-26869-6.Peer-Reviewed Original ResearchConceptsVibrio cholerae biofilmsSurface-attached aggregatesBacterial communitiesCell-surface interactionsDevelopmental processesBiofilm developmentBacterial cellsCell reorientationExtracellular matrixNonadherent mutantsDifferential growthBacterial biofilmsMechanical forcesBiofilmsBacterial growthDifferential orderingCellsGrowthMutantsLarge collectionMorphogenesis and cell ordering in confined bacterial biofilms
Zhang Q, Li J, Nijjer J, Lu H, Kothari M, Alert R, Cohen T, Yan J. Morphogenesis and cell ordering in confined bacterial biofilms. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2107107118. PMID: 34330824, PMCID: PMC8346881, DOI: 10.1073/pnas.2107107118.Peer-Reviewed Original ResearchConceptsInherent developmental programsBiofilm-dwelling cellsBiofilm-forming speciesSingle-cell imagingDevelopmental programSpecific matrix componentsCell orderingBiofilm developmentSpherical biofilmsBacterial cellsCell communityExtracellular matrixBiofilm morphologyBacterial biofilmsBiophysical mechanismsMatrix componentsHost tissuesBiofilmsBiofilm growthStiff gelsCellsMorphogenesisMutagenesisSpeciesVibrio
2026
Crystal structure of Vibrio cholerae polysaccharide lyase RbmB bound to Vibrio polysaccharide (VPS) fragments provides insights into substrate recognition and cleavage.
Weerasekera R, Moreau A, Huang X, Potapova A, Schwechheimer C, Kandel R, Huynh Y, Cannizzo O, Gordon R, Gerace E, Hinbest A, Yang Y, Jiang X, Woods R, Yan J, Yildiz F, Olson R. Crystal structure of Vibrio cholerae polysaccharide lyase RbmB bound to Vibrio polysaccharide (VPS) fragments provides insights into substrate recognition and cleavage. Proceedings Of The National Academy Of Sciences Of The United States Of America 2026, 123: e2534280123. PMID: 41805565, PMCID: PMC12993940, DOI: 10.1073/pnas.2534280123.Peer-Reviewed Original ResearchConceptsVibrio polysaccharideVibrio choleraeComplementary functional analysesHuman pathogenMutant proteins in vitroProduction of biofilmEnzyme-product complexProteins in vitroSequence conservationSubstrate recognitionSalt bridge interactionsBiofilm dispersalCrystal structureCleavage siteAccessory proteinsBiofilm matrixScissile bondArginine residuesRbmBL-guloseMolecular dynamics simulationsBiofilmEnvironmental stressorsCleavage mechanismExopolysaccharideConformations and sequence determinants in the lipid binding of an adhesive peptide derived from Vibrio cholerae biofilms.
Huang X, Prasad R, Saluja S, Yang Y, Yan Q, Shuster S, Karatekin E, Olson R, Lin C, Davis C, Jiang X, Zhou H, Yan J. Conformations and sequence determinants in the lipid binding of an adhesive peptide derived from Vibrio cholerae biofilms. PLOS Pathogens 2026, 22: e1013990. PMID: 41712669, PMCID: PMC12965690, DOI: 10.1371/journal.ppat.1013990.Peer-Reviewed Original ResearchConceptsCausal agentSensitive to membrane curvatureAromatic-rich motifsSurvival of pathogenic bacteriaSurvival of V. choleraeVibrio cholerae biofilmsAdhesion peptidesBiofilm contextVibrio speciesPseudo-repeatsHost colonizationBiofilm lifestyleAbiotic surfacesV. choleraeAcidic loopB-hairpinSequence determinationCore motifPandemic choleraVibrio choleraeGenetic approachesLipid bindingMembrane curvatureMembrane insertionSecondary structure
2025
Vibrio cholerae adhesin-derived peptides mediate strong pull-off forces in aqueous media with high ionic strength
Ahmed S, Zhai S, Huang X, Sulaja S, Adewole A, Ioffe A, Merg A, Yan J, Andresen Eguiluz R. Vibrio cholerae adhesin-derived peptides mediate strong pull-off forces in aqueous media with high ionic strength. Colloids And Surfaces B Biointerfaces 2025, 260: 115390. PMID: 41478156, DOI: 10.1016/j.colsurfb.2025.115390.Peer-Reviewed Original ResearchStructures of the sheathed flagellum reveal mechanisms of assembly and rotation in Vibrio cholerae
Guo W, Zhang S, Park J, Stanton V, Asp M, Herrera H, Tai J, Yue J, Wang J, Guo J, Kumar R, Botting J, Wu S, Yan J, Klose K, Yildiz F, Liu J. Structures of the sheathed flagellum reveal mechanisms of assembly and rotation in Vibrio cholerae. Nature Microbiology 2025, 10: 3305-3314. PMID: 41174224, DOI: 10.1038/s41564-025-02161-x.Peer-Reviewed Original ResearchConceptsFlagellar filamentsV. choleraeVibrio choleraeMembranous sheathMotility of V. choleraeCryo-electron microscopy single-particle analysisMechanism of assemblyComplex life cyclePolar flagellumSheathed flagellaCell polesFlagellin proteinFilament assemblySingle-particle analysisOuter membraneSheathed filamentsFlagellin levelsIntact bacteriaSupercoiled filamentsMolecular geneticsFlagellinConformational changesFlagellaInfectious capabilityFluorescence microscopyHuman antibody targeting Vibrio cholerae O1 O-specific polysaccharide induces an amotile hypovirulent bacterial phenotype: mechanism of protection against cholera
Verma S, Cetinbas M, Kelly M, Senger S, Faherty C, Janardhanan J, Wagh C, Bhuiyan T, Chowdhury F, Khan A, Akter A, Charles R, Harris J, Calderwood S, Wrammert J, Waldor M, Asp M, Tai J, Yan J, Xu P, Kováč P, Sadreyev R, Qadri F, Ryan E. Human antibody targeting Vibrio cholerae O1 O-specific polysaccharide induces an amotile hypovirulent bacterial phenotype: mechanism of protection against cholera. MBio 2025, 16: e02235-25. PMID: 40937849, PMCID: PMC12505965, DOI: 10.1128/mbio.02235-25.Peer-Reviewed Original ResearchAnti-OSP antibodiesO-specific polysaccharideVibrio cholerae,Bacterial motilityLevels of cyclic di-GMPInhibition of bacterial motilityEnteroid modelCyclic di-GMPCholera toxin detectionIntestinal surfaceCulture-based assaysInhibition of motilityVirulence programInfect humansRegulon expressionDi-GMPVirulence genesVirulence mechanismsBacterial phenotypesPathogen physiologyPresence of mucinExtracellular matrix componentsBiofilm formationBacterial metabolismIntestinal mucus
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- Reconstructed 3D image of a Vibrio cholerae biofilm cluster. Color corresponds to height.
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