Leonor Teles, PhD
Postdoctoral AssociateAbout
Research
Publications
2025
Decellularized lymph node sections with preserved extracellular matrix for stromal cell culture
Esparza E, Teles L, Fedotova A, Dehaseth N, Sayegh M, Hernandez A, Ho L, Ziebarth N, Tomei A. Decellularized lymph node sections with preserved extracellular matrix for stromal cell culture. Scientific Reports 2025, 16: 107. PMID: 41286062, PMCID: PMC12764942, DOI: 10.1038/s41598-025-28782-0.Peer-Reviewed Original ResearchConceptsExtracellular matrixExtracellular matrix proteinsFibroblastic reticular cellsFlow cytometric analysisCellular behaviorExtracellular matrix featuresCell-free scaffoldsCytometric analysisCell culturesHuman tissuesCell interactionsAdaptive immunityCellsCells co-culturedCo-CultureDecellularization methodsDense scaffoldsStromal cell culturesCell seedingImmune cell interactionsPDGFRA expressionImmunofluorescence stainingLymph node sectionsNative tissueEngineered macroporous gelatin scaffolds enhance lymph node fibroblastic reticular cell identity and enable diabetogenic T cell immunomodulation
Teles L, Beatty L, Hernandez A, Mendoza M, Wilkes Z, Dominick V, Telias M, Miller B, Huang C, Genzano C, Andreopoulos F, Dauer E, Creusot R, Tomei A. Engineered macroporous gelatin scaffolds enhance lymph node fibroblastic reticular cell identity and enable diabetogenic T cell immunomodulation. Biomaterials 2025, 324: 123460. PMID: 40472409, PMCID: PMC12823137, DOI: 10.1016/j.biomaterials.2025.123460.Peer-Reviewed Original ResearchConceptsGelatin scaffoldsAg-presenting cellsFibroblastic reticular cellsCo-culture of T cellsPre-diabetic NOD miceT-cell engagersNOD mouse modelT-cell immunomodulationPore sizeImmunomodulation in vitroBiological gelsPersistent presentationPhenotypic marker expressionNon-inflamed sitesType 1 diabetesIn vivo survivalNOD miceGraft rejectionExtracellular matrix secretionT cellsAutoimmune diseasesRegulatory phenotypeGFP-luciferaseMouse modelRelevant antigens
2024
2016-LB: Uptake Kinetics and Presentation of Donor Alloantigen by Lymph Node Stromal Cells for Attenuating the Alloimmune Response in Pancreatic Islet Transplantation
LI C, MULLIGAN C, TELES L, GONZALEZ G, UMLAND O, TOMEI A. 2016-LB: Uptake Kinetics and Presentation of Donor Alloantigen by Lymph Node Stromal Cells for Attenuating the Alloimmune Response in Pancreatic Islet Transplantation. Diabetes 2024, 73 DOI: 10.2337/db24-2016-lb.Peer-Reviewed Original ResearchFibroblastic reticular cellsRecipient alloimmune responsesAlloimmune responseT cellsPancreatic islet transplantationIn vitro co-culture studiesAlloreactive T cell activationLymph Node Stromal CellsRecipient T cellsAutoreactive T cellsAntigen-presenting cellsT cell activationIslet transplantationJuvenile Diabetes Research FoundationCo-culture studiesStromal cell subtypesDonor alloantigensDonor antigensLifelong immunosuppressionInterferon-gAntigen presentationExogenous antigensCell antigensDose-dependentlyCo-Transplantation
2023
Islet Immunoengineering
Teles L, Li C, Wilkes Z, Stock A, Tomei A. Islet Immunoengineering. 2023, 317-359. DOI: 10.1007/978-3-031-41943-0_15.Peer-Reviewed Original ResearchStem cell-derived isletsIslet transplantationSevere casesPatients' qualityPost-transplant immunosuppressionCases of T1DRecurrence of autoimmunityPatients' quality of lifeImmunomodulatory drug deliveryType 1 diabetesSystemic immunosuppressionApplication of islet transplantationIntrahepatic islet transplantationB cellsHepatic portal veinPortal veinIslet rejectionLong-term functionIslet allotransplantsMetabolic controlTransplantationInsulin independenceQuality of lifeClinical translationPatients