Siqi Huang
Postdoctoral AssociateAbout
Research
Publications
2026
Optimization of trap column properties and loading conditions for proteome profiling of single-cell-level sample inputs
Huang S, Truong T, Wang C, Xie X, Lin H, Kelly R. Optimization of trap column properties and loading conditions for proteome profiling of single-cell-level sample inputs. Analytical And Bioanalytical Chemistry 2026, 1-10. PMID: 41857229, PMCID: PMC13118651, DOI: 10.1007/s00216-026-06440-2.Peer-Reviewed Original ResearchNanoflow liquid chromatography-mass spectrometryLoading flow rateLiquid chromatography-mass spectrometryColumn inner diameterIdentification depthTrap columnAnalytical columnSample cleanupChromatography-mass spectrometryChromatographic qualityHigh-throughput conditionsProteome depthColumn longevityProteome coveragePeak areaPeak widthPacking materialBiological samplesColumn propertiesSample concentrationProteomic profilingParticle sizeColumn geometryHigh-throughput proteomicsColumnModification of a Low‐Cost Pipetting Robot for Nanoliter Liquid Handling and Autosampling for Liquid Chromatography‐Mass Spectrometry
Axtell N, Webber K, Truong T, Lin H, Sandberg A, Martin S, Xie X, Huang S, Wang C, Kelly R. Modification of a Low‐Cost Pipetting Robot for Nanoliter Liquid Handling and Autosampling for Liquid Chromatography‐Mass Spectrometry. Journal Of Separation Science 2026, 49: e70379-e70379. PMID: 41742430, PMCID: PMC13118588, DOI: 10.1002/jssc.70379.Peer-Reviewed Original ResearchSample preparationNano-LC-MSLiquid chromatography-mass spectrometryChromatography-mass spectrometryOpen-source platformSample processing throughputSensitive sample preparationLiquid handlingLC applicationsSeparation platformLow-volume dispensingAutomated liquid handlingLiquid handling platformCustom functionsNanoliter liquid handlingTwo-position valvesProcessing throughputNanoliter rangePipetting robotHuman error
2025
Trends in Mass Spectrometry-Based Single-Cell Proteomics
Sanchez-Avila X, de Oliveira R, Huang S, Wang C, Kelly R. Trends in Mass Spectrometry-Based Single-Cell Proteomics. Analytical Chemistry 2025, 97: 5893-5907. PMID: 40091206, PMCID: PMC12003028, DOI: 10.1021/acs.analchem.5c00661.Peer-Reviewed Original Research
2024
Gradient-Elution Nanoflow Liquid Chromatography Without a Binary Pump: Smoothed Step Gradients Enable Reproducible, Sensitive, and Low-Cost Separations for Single-Cell Proteomics
Webber K, Huang S, Lin H, Hunter T, Tsang J, Jayatunge D, Andersen J, Kelly R. Gradient-Elution Nanoflow Liquid Chromatography Without a Binary Pump: Smoothed Step Gradients Enable Reproducible, Sensitive, and Low-Cost Separations for Single-Cell Proteomics. Molecular & Cellular Proteomics 2024, 23: 100880. PMID: 39536954, PMCID: PMC11667035, DOI: 10.1016/j.mcpro.2024.100880.Peer-Reviewed Original ResearchConceptsBinary pumpTimsTOF mass spectrometerMass spectrometry-based proteomic profilingNanoflow liquid chromatographyLiquid chromatography separationTrace analytesHeLa digestGradient elutionLow-cost separationMass spectrometerSmooth gradient profileSolvent strengthMobile phaseSingle-cell proteomicsChromatography separationLiquid chromatographyGradient profileThousands of proteinsCell benefitsSeparationFlow rateLow costTimsTOFSolventStep gradientMulticolumn Nanoflow Liquid Chromatography with Accelerated Offline Gradient Generation for Robust and Sensitive Single-Cell Proteome Profiling
Xie X, Truong T, Huang S, Johnston S, Hovanski S, Robinson A, Webber K, Lin H, Mun D, Pandey A, Kelly R. Multicolumn Nanoflow Liquid Chromatography with Accelerated Offline Gradient Generation for Robust and Sensitive Single-Cell Proteome Profiling. Analytical Chemistry 2024, 96: 10534-10542. PMID: 38915247, PMCID: PMC11482043, DOI: 10.1021/acs.analchem.4c00878.Peer-Reviewed Original ResearchConceptsAnalytical columnSample loopMobile phase gradientNanoflow liquid chromatographyProteomes of single cellsBottom-up proteomicsTrap columnNanoLC systemColumn lifetimePeptide separationMultiple myeloma cell linesPeak capacitySingle cellsHydrophobic speciesBinary pumpMyeloma cell linesSingle-cell proteomic profilingProteomic profilingLiquid chromatographyGradient generatorCell linesProteomicsColumn replacementColumnStorage loopOpen-tubular trap columns: towards simple and robust liquid chromatography separations for single-cell proteomics
Webber K, Huang S, Truong T, Heninger J, Gregus M, Ivanov A, Kelly R. Open-tubular trap columns: towards simple and robust liquid chromatography separations for single-cell proteomics. Molecular Omics 2024, 20: 184-191. PMID: 38353725, PMCID: PMC10963139, DOI: 10.1039/d3mo00249g.Peer-Reviewed Original ResearchConceptsTrap columnChromatographic performanceAnalytical columnSingle-cell proteomicsNanoflow liquid chromatography-mass spectrometrySample loading capacityLiquid chromatography-mass spectrometryLiquid chromatography separationNarrow-bore columnsBackflush modeChromatography-mass spectrometrySample cleanupLow back pressureCleanup stepChromatography separationColumn cloggingSample loopStationary phaseAnalysis of trace samplesSample mixturePeak widthTrace samplesPacked columnColumnSeparation
2022
Fluorescence recovery based on synergetic effect for ALP detection
Huang S, Yang W, Ye S, Cao S, Li Y, Wei Z, Yan Ngai K, Dai J, Mao G, Ma Y. Fluorescence recovery based on synergetic effect for ALP detection. Spectrochimica Acta Part A Molecular And Biomolecular Spectroscopy 2022, 280: 121550. PMID: 35777229, DOI: 10.1016/j.saa.2022.121550.Peer-Reviewed Original ResearchConceptsALP-catalyzed hydrolysisDetection of alkaline phosphataseOne-pot water bath methodSensitive detectionSensitive detection of alkaline phosphataseQuantum dotsWater bath methodAlkaline phosphatase detectionAscorbic acid phosphateALP detectionDetection limitFluorescence recoverySynergetic effectBiomarkers associated with diabetesGood stabilityBath methodDetection sensitivityHuman serumCdZnSe quantum dotsAscorbic acidFluorescence
2020
Novel Method of Clickable Quantum Dot Construction for Bioorthogonal Labeling
Mao G, Ma Y, Wu G, Du M, Tian S, Huang S, Ji X, He Z. Novel Method of Clickable Quantum Dot Construction for Bioorthogonal Labeling. Analytical Chemistry 2020, 93: 777-783. PMID: 33300344, DOI: 10.1021/acs.analchem.0c03078.Peer-Reviewed Original ResearchConceptsMetabolic oligosaccharide engineeringQuantum dotsGlucose oxidaseBioorthogonal functional groupsStability of quantum dotsActivity of glucose oxidaseModerate reaction conditionsLabeling of biomoleculesHeLa cellsQD quantum yieldDNA functionInteraction of SHBioorthogonal labelingPaper-based analytical devicesReaction conditionsBiomolecule labelingQuantum yieldMetal ionsEffective labelingBioorthogonal chemistryFunctional groupsSite-specificCovalent couplingAnalytical devicesHydrodynamic diameter
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